EPB41 (NM_203342) Human Untagged Clone

CAT#: SC310203

EPB41 (untagged)-Human erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked) (EPB41), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPB41
• Synonyms: 4.1R; EL1; HE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310203 representing NM_203342.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCACTGCAAGGTTTCTTTGTTGGATGACACAGTTTATGAATGTGTTGTGGAGAAACATGCTAAGGGA
  CAAGATTTGCTTAAACGAGTATGTGAGCATCTCAATCTTTTGGAAGAAGACTATTTTGGTCTAGCCATT
  TGGGATAACGCAACCTCTAAGACATGGCTGGATTCCGCCAAAGAAATAAAAAAGCAGGTTCGTGGTGTC
  CCTTGGAATTTTACATTTAATGTAAAGTTTTATCCACCTGACCCAGCACAGTTAACAGAAGACATAACA
  AGATATTATTTATGTCTTCAGCTTCGGCAGGACATAGTTGCAGGACGTCTGCCCTGTTCCTTTGCAACC
  TTAGCATTATTAGGTTCTTACACCATCCAGTCTGAACTGGGAGACTACGACCCAGAACTCCATGGCGTG
  GATTATGTTAGTGATTTTAAACTGGCCCCGAATCAGACCAAGGAACTTGAAGAGAAGGTCATGGAACTG
  CATAAGTCATACAGGTCCATGACTCCAGCTCAGGCTGACTTGGAGTTTCTTGAGAATGCCAAAAAGTTG
  TCTATGTATGGAGTTGATCTTCATAAAGCAAAGGACTTGGAAGGAGTAGATATCATCCTAGGTGTCTGC
  TCTAGTGGCCTTCTGGTTTACAAAGATAAGCTGAGAATTAACCGCTTCCCTTGGCCCAAAGTGCTGAAG
  ATTTCTTATAAACGTAGTAGCTTTTTCATCAAGATTCGGCCTGGAGAGCAAGAGCAGTATGAAAGTACC
  ATCGGATTCAAACTTCCCAGTTACCGAGCAGCTAAGAAATTATGGAAAGTCTGTGTAGAACATCACACG
  TTTTTCAGATTGACATCTACAGACACCATTCCCAAAAGCAAATTTCTTGCGCTAGGATCCAAATTTCGA
  TACAGTGGCCGGACTCAAGCTCAGACCAGGCAAGCTAGTGCTCTAATTGACAGGCCTGCCCCACACTTC
  GAGCGTACAGCAAGTAAACGGGCGTCCCGGAGCCTCGATGGAGCAGCAGCTGTCGATTCGGCAGACCGA
  AGTCCTCGGCCCACTTCTGCACCTGCCATTACTCAGGGTCAGGTTGCAGAAGGTGGCGTCCTAGATGCC
  TCTGCTAAAAAAACAGTGGTCCCTAAAGCACAGAAGGAAACAGTGAAGGCTGAAGTGAAAAAGGAAGAC
  GAGCCACCTGAGCAAGCTGAGCCAGAGCCCACAGAAGCATGGAAGGTGGAAAAAACCCACATCGAGGTG
  ACAGTACCCACCTCAAATGGTGACCAAACACAGAAAAAGAGAGAAAGACTAGATGGTGAAAACATTTAT
  ATCAGACATAGCAATTTAATGTTGGAGGATTTAGACAAGAGTCAAGAGGAGATCAAAAAACATCATGCC
  AGCATCAGTGAGCTGAAAAAGAACTTCATGGAGTCTGTACCAGAACCACGGCCTAGTGAATGGGATAAA
  CGCTTATCCACTCACTCACCCTTCCGAACTCTTAACATCAATGGGCAAATCCCCACAGGAGAAGGACCT
  CCCCTGGTGAAGACACAAACTGTCACCATCTCAGATAATGCCAATGCTGTGAAAAGTGAAATCCCAACC
  AAAGACGTCCCTATTGTCCACACTGAGACCAAGACCATCACTTATGAGGCTGCCCAGACTGACGACAAC
  AGTGGAGACTTGGACCCAGGAGTCTTGCTGACAGCTCAAACTATCACATCTGAGACCCCAAGCAGCACC
  ACCACAACTCAAATTACCAAGACTGTAAAAGGTGGGATTTCAGAGACACGTATTGAAAAGAGAATTGTG
  ATCACAGGAGATGCTGATATTGACCATGATCAGGTCCTTGTACAAGCCATCAAGGAGGCAAAGGAGCAG
  CACCCAGACATGTCAGTGACCAAGGTGGTCGTCCACCAGGAGACCGAGATTGCTGATGAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_203342
• Insert Size: 1926 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203342.2
• RefSeq Size: 5999 bp
• RefSeq ORF: 1926 bp
• Locus ID: 2035
• UniProt ID: P11171
• Cytogenetics: 1p35.3
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• MW: 72 kDa
• Gene Summary: The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]
  Transcript Variant: This variant (4) contains an additional exon at the 5' end, and is missing an internal, in-frame coding exon compared to variant 1. This results in translation start from a downstream AUG and a shorter isoform (4) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.