LAMA3 (NM_198129) Human Untagged Clone

CAT#: SC309364

LAMA3 (untagged)-Human laminin, alpha 3 (LAMA3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LAMA3
• Synonyms: BM600; E170; LAMNA; LOCS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309364 representing NM_198129.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGCGGCCGCGCGGCCTCGGGGTCGGGCACTGGGGCCAGTACTGCCGCCGACGCCGCTGCTCCTG
  CTGGTACTGCGGGTGCTGCCAGCCTGCGGGGCGACCGCTCGGGATCCCGGGGCCGCGGCCGGGCTCAGC
  CTTCACCCGACTTACTTCAACCTGGCCGAGGCGGCGAGGATTTGGGCCACCGCCACCTGCGGGGAGAGG
  GGACCCGGCGAGGGGAGGCCCCAGCCCGAGCTCTACTGCAAGTTGGTCGGGGGCCCCACCGCCCCAGGC
  AGCGGCCACACCATCCAGGGCCAGTTCTGTGACTATTGCAATTCTGAAGACCCCAGGAAAGCACATCCT
  GTCACCAATGCCATCGATGGATCTGAACGTTGGTGGCAAAGCCCTCCCCTGTCCTCAGGCACACAGTAC
  AACAGAGTCAACCTCACCTTGGATCTGGGGCAGCTCTTCCATGTGGCCTATATTTTAATCAAATTTGCA
  AATTCTCCTCGCCCTGATCTTTGGGTCTTGGAAAGATCTGTAGACTTTGGAAGCACCTACTCACCATGG
  CAATATTTTGCTCATTCTAAAGTAGACTGTTTAAAAGAATTTGGGCGGGAGGCAAATATGGCTGTCACC
  CGGGATGATGATGTACTTTGTGTTACTGAATATTCCCGTATTGTACCTTTGGAAAATGGTGAGGTTGTG
  GTGTCCTTGATAAACGGTCGTCCAGGTGCAAAAAATTTTACTTTCTCTCACACCCTGAGGGAGTTTACC
  AAGGCAACAAACATCCGCTTGCGTTTTCTTAGAACCAATACGCTTCTTGGACACCTCATCTCCAAAGCC
  CAGCGAGATCCAACTGTCACTCGGCGGTATTATTACAGCATAAAGGACATCAGCATTGGTGGGCAGTGT
  GTTTGCAATGGCCATGCTGAAGTGTGCAATATAAACAATCCTGAAAAACTGTTTCGGTGTGAATGCCAG
  CACCACACCTGTGGGGAGACGTGTGATCGCTGCTGCACAGGGTACAATCAGAGGCGCTGGCGGCCCGCC
  GCTTGGGAGCAGAGCCACGAGTGTGAAGCATGCAACTGCCACGGCCATGCCAGCAACTGTTACTATGAT
  CCAGATGTTGAGCGGCAGCAGGCAAGCTTGAATACCCAGGGCATCTATGCTGGTGGAGGGGTCTGCATT
  AACTGTCAGCACAACACAGCTGGAGTAAACTGTGAACAGTGTGCTAAGGGCTATTACCGCCCTTATGGG
  GTTCCAGTGGATGCCCCTGATGGCTGCATCCCCTGCAGCTGTGACCCTGAGCATGCGGATGGCTGTGAA
  CAGGGTTCAGGCCGCTGTCACTGCAAGCCAAATTTCCACGGAGACAACTGTGAGAAGTGTGCAATTGGA
  TACTACAATTTCCCATTTTGCTTGAGAATTCCCATTTTTCCTGTTTCTACACCAAGTTCAGAAGATCCA
  GTAGCTGGAGATATAAAAGGGTGTGACTGTAATCTGGAAGGTGTTCTCCCTGAAATATGTGATGCCCAC
  GGACGGTGCCTGTGCCGCCCTGGGGTTGAGGGCCCTCGATGTGATACCTGCCGCTCTGGTTTCTACTCA
  TTCCCTATTTGCCAAGCCTGCTGGTGTTCAGCCCTTGGATCCTACCAGATGCCCTGCAGCTCAGTGACT
  GGACAGTGTGAATGTCGGCCAGGAGTTACAGGACAGCGGTGTGACAGGTGTCTCTCAGGAGCTTATGAT
  TTCCCCCACTGCCAAGGTTCCAGCAGTGCTTGTGACCCAGCTGGTACCATCAACTCCAATTTGGGGTAT
  TGCCAATGCAAGCTTCATGTTGAAGGTCCTACTTGTAGCCGCTGCAAACTGTTATATTGGAATCTGGAC
  AAAGAAAACCCCAGTGGATGTTCAGAATGCAAGTGCCATAAGGCGGGAACAGTGAGTGGAACTGGAGAG
  TGTAGGCAGGGAGATGGTGACTGTCACTGCAAGTCCCATGTGGGTGGCGATTCCTGCGACACCTGTGAA
  GATGGATATTTTGCTTTGGAAAAGAGCAATTACTTTGGGTGTCAAGGGTGTCAGTGTGACATTGGTGGG
  GCATTGTCCTCCATGTGCAGTGGGCCCTCGGGAGTGTGCCAGTGCCGAGAGCATGTCGTGGGAAAGGTG
  TGCCAGCGGCCTGAAAACAACTACTATTTCCCAGATTTGCATCATATGAAGTATGAGATTGAAGACGGC
  AGCACACCTAATGGGAGAGACCTTCGATTTGGATTTGATCCGCTGGCATTTCCTGAGTTTAGCTGGAGA
  GGATATGCCCAAATGACCTCAGTACAGAATGATGTAAGAATAACATTGAATGTAGGGAAGTCAAGTGGC
  TCCTTGTTTCGTGTTATTCTGAGATACGTTAACCCTGGAACTGAAGCAGTATCTGGCCATATAACTATT
  TATCCATCCTGGGGTGCTGCTCAAAGCAAAGAGATCATCTTCCTGCCGAGTAAGGAGCCAGCCTTTGTC
  ACTGTCCCTGGAAATGGTTTTGCAGACCCATTTTCAATCACACCAGGAATATGGGTTGCTTGTATTAAG
  GCAGAAGGAGTCCTTCTGGATTACCTGGTGCTGCTCCCCAGGGACTACTATGAAGCCTCTGTACTGCAG
  CTGCCAGTCACAGAACCATGTGCCTACGCAGGACCTCCCCAAGAAAATTGCTTACTCTACCAGCATTTG
  CCAGTGACCAGATTCCCCTGTACCCTGGCTTGTGAGGCCAGACACTTCCTGCTTGATGGGGAGCCAAGA
  CCCGTGGCAGTGAGGCAGCCCACACCTGCACACCCTGTCATGGTGGACCTCAGCGGGAGAGAGGTGGAA
  TTGCATCTGCGGCTGCGCATCCCACAGGTTGGCCACTACGTGGTTGTGGTCGAGTATTCCACGGAGGCA
  GCTCAGCTGTTTGTGGTTGATGTGAATGTGAAGAGCTCCGGGTCTGTTCTGGCAGGCCAGGTGAACATT
  TACAGCTGCAACTACAGTGTTCTCTGCCGGAGTGCTGTGATTGATCACATGAGCCGCATCGCCATGTAT
  GAGCTATTGGCAGATGCAGACATTCAGCTCAAGGGACACATGGCCCGATTCCTTCTGCATCAAGTTTGT
  ATCATACCTATTGAAGAATTCTCAGCTGAGTATGTGAGACCACAAGTCCACTGCATTGCCAGTTATGGG
  CGATTTGTCAATCAAAGTGCCACCTGTGTCTCCTTGGCCCATGAAACTCCTCCAACAGCATTAATTTTG
  GATGTTCTAAGTGGCAGGCCTTTCCCTCACCTGCCCCAGCAGTCGTCACCTTCTGTTGATGTTCTTCCT
  GGGGTCACCTTGAAGGCACCGCAGAATCAAGTGACCCTGAGAGGACGTGTACCACACCTGGGCCGATAC
  GTCTTTGTCATCCATTTTTACCAAGCAGCGCACCCGACGTTTCCCGCGCAGGTGTCGGTGGATGGCGGG
  TGGCCACGGGCAGGCTCCTTCCATGCCTCTTTTTGCCCCCATGTGCTTGGCTGCCGGGATCAAGTGATT
  GCCGAAGGCCAGATTGAGTTTGACATCTCAGAGCCTGAAGTGGCCGCAACTGTGAAGGTTCCAGAAGGA
  AAGTCCTTGGTTTTGGTCCGTGTTCTAGTGGTGCCTGCAGAAAACTATGACTACCAAATACTTCACAAA
  AAATCCATGGACAAGTCACTCGAGTTTATCACCAATTGTGGAAAAAACAGCTTTTACCTTGACCCCCAG
  ACAGCCTCCAGATTCTGTAAGAATTCCGCCAGGTCCCTGGTGGCCTTTTACCACAAGGGCGCCCTGCCT
  TGTGAGTGCCACCCCACTGGGGCCACCGGCCCTCACTGCAGCCCTGAGGGTGGGCAGTGCCCATGCCAG
  CCCAACGTCATCGGGCGGCAGTGCACCCGCTGTGCAACAGGCCACTACGGATTCCCACGCTGCAAGCCG
  TGCAGCTGTGGTCGGCGCCTTTGTGAAGAGATGACGGGGCAGTGCCGCTGCCCTCCCCGCACGGTCAGG
  CCCCAGTGTGAGGTGTGTGAGACACACTCATTCAGCTTCCACCCCATGGCCGGCTGCGAAGGCTGCAAC
  TGTTCCAGGAGGGGCACCATCGAGGCTGCCATGCCGGAGTGTGACCGGGACAGCGGGCAGTGCAGATGC
  AAGCCCAGAATCACAGGGCGGCAGTGTGACCGATGTGCTTCCGGGTTTTACCGCTTTCCTGAGTGTGTT
  CCCTGCAATTGCAACAGAGATGGGACTGAGCCAGGAGTGTGTGACCCAGGGACCGGGGCTTGCCTCTGC
  AAGGAAAATGTAGAAGGCACAGAGTGTAATGTGTGTCGAGAAGGCTCATTCCATTTGGACCCAGCCAAT
  CTCAAGGGTTGTACCAGCTGTTTCTGTTTTGGAGTAAATAATCAATGTCACAGCTCACATAAGCGAAGG
  ACTAAGTTTGTGGATATGCTGGGCTGGCACCTGGAGACAGCAGACAGAGTGGACATCCCTGTCTCTTTC
  AACCCAGGCAGCAACAGTATGGTGGCGGATCTCCAGGAGCTGCCCGCAACCATCCACAGCGCGTCCTGG
  GTCGCACCCACCTCCTACCTGGGGGACAAGGTTTCTTCATATGGTGGTTACCTCACTTACCAAGCCAAG
  TCCTTTGGCTTGCCTGGCGACATGGTTCTTCTGGAAAAGAAGCCGGATGTACAGCTCACTGGTCAGCAC
  ATGTCCATCATCTATGAGGAGACAAACACCCCACGGCCAGACCGGCTGCATCATGGACGAGTGCACGTG
  GTCGAGGGAAACTTCAGACATGCCAGCAGCCGTGCCCCAGTGTCTAGGGAGGAGCTGATGACAGTGCTG
  TCTAGACTGGCAGATGTGCGCATCCAAGGCCTCTACTTCACAGAGACTCAAAGGCTCACCCTGAGCGAG
  GTGGGGCTAGAGGAAGCCTCTGACACAGGAAGTGGGCGCATAGCACTTGCTGTGGAAATCTGTGCCTGC
  CCCCCTGCCTACGCTGGTGACTCTTGTCAGGGTTGTAGCCCTGGATACTATCGGGATCATAAAGGCTTG
  TATACCGGACGGTGTGTTCCCTGCAATTGCAACGGACATTCAAATCAATGCCAGGATGGCTCAGGCATA
  TGTGTTAACTGTCAGCACAACACCGCGGGAGAGCACTGTGAACGCTGCCAGGAGGGCTACTATGGCAAC
  GCCGTCCACGGATCCTGCAGGGCCTGCCCATGTCCTCACACTAACAGCTTTGCCACTGGCTGTGTGGTG
  AATGGGGGAGACGTGCGGTGCTCCTGCAAAGCTGGGTACACAGGAACACAGTGTGAAAGGTGTGCACCG
  GGATATTTCGGGAATCCCCAGAAATTCGGAGGTAGCTGCCAACCATGCAGTTGTAACAGCAATGGCCAG
  CTGGGCAGCTGTCATCCCCTGACTGGAGACTGCATAAACCAAGAACCCAAAGATAGCAGCCCTGCAGAA
  GAATGTGATGATTGCGACAGCTGTGTGATGACCCTCCTGAACGACCTGGCCACCATGGGCGAGCAGCTC
  CGCCTGGTCAAGTCTCAGCTGCAGGGCCTGAGTGCCAGCGCAGGGCTTCTGGAGCAGATGAGGCACATG
  GAGACCCAGGCCAAGGACCTGAGGAATCAGTTGCTCAACTACCGTTCTGCCATTTCAAATCATGGATCA
  AAAATAGAAGGCCTGGAAAGAGAACTGACTGATTTGAATCAAGAATTTGAGACTTTGCAAGAAAAGGCT
  CAAGTAAATTCCAGAAAAGCACAAACATTAAACAACAATGTTAATCGGGCAACACAAAGCGCAAAAGAA
  CTGGATGTGAAGATTAAAAATGTCATCCGGAATGTGCACATTCTTTTAAAGCAGATCTCTGGGACAGAT
  GGAGAGGGAAACAACGTGCCTTCAGGTGACTTTTCCAGAGAGTGGGCTGAAGCCCAGCGCATGATGAGG
  GAACTGCGGAACAGGAACTTTGGAAAGCACCTCAGAGAAGCAGAAGCTGATAAAAGGGAGTCGCAGCTC
  TTGCTGAACCGGATAAGGACCTGGCAGAAAACCCACCAGGGGGAGAACAATGGGCTTGCTAACAGTATC
  CGGGATTCTTTAAATGAATACGAAGCCAAACTCAGTGACCTTCGTGCTCGGCTGCAGGAGGCAGCTGCC
  CAAGCCAAGCAGGCAAATGGCTTGAACCAAGAAAACGAGAGAGCTTTGGGAGCCATTCAGAGACAAGTG
  AAAGAAATAAATTCCCTGCAGAGTGATTTCACCAAGTATCTAACCACTGCAGACTCATCTTTGTTGCAA
  ACCAACATTGCGCTGCAGCTGATGGAGAAAAGCCAGAAGGAATATGAAAAATTAGCTGCCAGTTTAAAT
  GAAGCAAGACAAGAACTAAGTGACAAAGTAAGAGAACTTTCCAGATCTGCTGGCAAAACATCCCTTGTG
  GAGGAGGCAGAAAAGCACGCGCGGTCCTTACAAGAGCTGGCAAAGCAGCTGGAAGAGATCAAGAGAAAC
  GCCAGCGGGGATGAGCTGGTGCGCTGTGCTGTGGATGCCGCCACCGCCTACGAGAACATCCTCAATGCC
  ATCAAAGCGGCCGAGGACGCAGCCAACAGGGCTGCCAGTGCATCTGAATCTGCCCTCCAGACAGTGATA
  AAGGAAGATCTGCCAAGAAAAGCTAAAACCCTGAGTTCCAACAGTGATAAACTGTTAAATGAAGCCAAG
  ATGACACAAAAGAAGCTAAAGCAAGAAGTCAGTCCAGCTCTCAACAACCTACAGCAAACCCTGAATATT
  GTGACAGTTCAGAAAGAAGTGATAGACACCAATCTCACAACTCTCCGAGATGGTCTTCATGGGATACAG
  AGAGGTGATATTGATGCTATGATCAGTAGTGCAAAGAGCATGGTCAGAAAGGCCAACGACATCACAGAT
  GAGGTTCTGGATGGGCTCAACCCCATCCAGACAGATGTGGAAAGAATTAAGGACACCTATGGGAGGACA
  CAGAACGAAGACTTCAAAAAGGCTCTGACTGATGCAGATAACTCGGTGAATAAGTTAACCAACAAACTA
  CCTGATCTTTGGCGCAAGATTGAAAGTATCAACCAACAGCTGTTGCCCTTGGGAAACATCTCTGACAAC
  ATGGACAGAATACGAGAACTAATTCAGCAGGCCAGAGATGCTGCCAGTAAGGTTGCTGTCCCCATGAGG
  TTCAATGGTAAATCTGGAGTCGAAGTCCGACTGCCAAATGACCTGGAAGATTTGAAAGGATATACATCT
  CTGTCCTTGTTTCTCCAAAGGCCCAACTCAAGAGAAAATGGGGGTACTGAGAATATGTTTGTGATGTAC
  CTTGGAAATAAAGATGCCTCCCGGGACTACATCGGCATGGCAGTTGTGGATGGCCAGCTCACCTGTGTC
  TACAACCTGGGGGACCGTGAGGCTGAACTCCAAGTGGACCAGATCTTGACCAAGAGTGAGACTAAGGAG
  GCAGTTATGGATCGGGTGAAATTTCAGAGAATTTATCAGTTTGCAAGGCTTAATTACACCAAAGGAGCC
  ACATCCAGTAAACCAGAAACACCCGGAGTCTATGACATGGATGGTAGAAATAGCAATACACTCCTTAAT
  TTGGATCCTGAAAATGTTGTATTTTATGTTGGAGGTTACCCACCTGATTTTAAACTTCCCAGTCGACTA
  AGTTTCCCTCCATACAAAGGTTGTATTGAATTAGATGACCTCAATGAAAATGTTCTGAGCTTGTACAAC
  TTCAAAAAAACATTCAATCTCAACACAACTGAAGTGGAGCCTTGTAGAAGGAGGAAGGAAGAGTCAGAC
  AAAAATTATTTTGAAGGTACGGGCTATGCTCGAGTTCCAACTCAACCACATGCTCCCATCCCAACCTTT
  GGACAGACAATTCAGACCACCGTGGATAGAGGCTTGCTGTTCTTTGCAGAAAACGGGGATCGCTTCATA
  TCTCTAAATATAGAAGATGGCAAGCTCATGGTGAGATACAAACTGAATTCAGAGCTACCAAAAGAGAGA
  GGAGTTGGAGACGCCATAAACAACGGCAGAGACCATTCGATTCAGATCAAAATTGGAAAACTCCAAAAG
  CGTATGTGGATAAATGTGGACGTTCAAAACACTATAATTGATGGTGAAGTATTTGATTTCAGCACATAT
  TATCTGGGAGGAATTCCAATTGCAATCAGGGAAAGATTTAACATTTCTACGCCTGCTTTCCGAGGCTGC
  ATGAAAAATTTGAAGAAAACCAGTGGTGTCGTTAGATTGAATGATACTGTGGGAGTAACCAAAAAGTGC
  TCGGAAGACTGGAAGCTTGTGCGATCTGCCTCATTCTCCAGAGGAGGACAATTGAGTTTCACTGATTTG
  GGCTTACCACCTACTGACCACCTCCAGGCCTCATTTGGATTTCAGACCTTTCAACCCAGTGGCATATTA
  TTAGATCATCAGACATGGACAAGGAACCTGCAGGTCACTCTGGAAGATGGTTACATTGAATTGAGCACC
  AGCGATAGCGGCGGCCCAATTTTTAAATCTCCACAGACGTATATGGATGGTTTACTGCATTATGTATCT
  GTAATAAGCGACAACTCTGGACTACGGCTTCTCATCGATGACCAGCTTCTGAGAAATAGCAAAAGGCTA
  AAACACATTTCAAGTTCCCGGCAGTCTCTGCGTCTGGGCGGGAGCAATTTTGAGGGTTGTATTAGCAAT
  GTTTTTGTCCAGAGGTTATCACTGAGTCCTGAAGTCCTAGATTTGACCAGTAACTCTCTCAAGAGAGAT
  GTGTCCCTGGGAGGCTGCAGTTTAAACAAACCACCTTTTCTAATGTTGCTTAAAGGTTCTACCAGGTTT
  AACAAGACCAAGACTTTTCGTATCAACCAGCTGTTGCAGGACACACCAGTGGCCTCCCCAAGGAGCGTG
  AAGGTGTGGCAAGATGCTTGCTCACCACTTCCCAAGACCCAGGCCAATCATGGAGCCCTCCAGTTTGGG
  GACATTCCCACCAGCCACTTGCTATTCAAGCTTCCTCAGGAGCTGCTGAAACCCAGGTCACAGTTTGCT
  GTGGACATGCAGACAACATCCTCCAGAGGACTGGTGTTTCACACGGGCACTAAGAACTCCTTTATGGCT
  CTTTATCTTTCAAAAGGACGTCTGGTCTTTGCACTGGGGACAGATGGGAAAAAATTGAGGATCAAAAGC
  AAGGAGAAATGCAATGATGGGAAATGGCACACGGTGGTGTTTGGCCATGATGGGGAAAAGGGGCGCTTG
  GTTGTGGATGGACTGAGGGCCCGGGAGGGAAGTTTGCCTGGAAACTCCACCATCAGCATCAGAGCGCCA
  GTTTACCTGGGATCACCTCCATCAGGGAAACCAAAGAGCCTCCCCACAAACAGCTTTGTGGGATGCCTG
  AAGAACTTTCAGCTGGATTCAAAACCCTTGTATACCCCTTCTTCAAGCTTCGGGGTGTCTTCCTGCTTG
  GGTGGTCCTTTGGAGAAAGGCATTTATTTCTCTGAAGAAGGAGGTCATGTCGTCTTGGCTCACTCTGTA
  TTGTTGGGGCCAGAATTTAAGCTTGTTTTCAGCATCCGCCCAAGAAGTCTCACTGGGATCCTAATACAC
  ATCGGAAGTCAGCCCGGGAAGCACTTATGTGTTTACCTGGAGGCAGGAAAGGTCACGGCCTCTATGGAC
  AGTGGGGCAGGTGGGACCTCAACGTCGGTCACACCAAAGCAGTCTCTGTGTGATGGACAGTGGCACTCG
  GTGGCAGTCACCATAAAACAACACATCCTGCACCTGGAACTGGACACAGACAGTAGCTACACAGCTGGA
  CAGATCCCCTTCCCACCTGCCAGCACTCAAGAGCCACTACACCTTGGAGGTGCTCCAGCCAATTTGACG
  ACACTGAGGATCCCTGTGTGGAAATCATTCTTTGGCTGTCTGAGGAATATTCATGTCAATCACATCCCT
  GTCCCTGTCACTGAAGCCTTGGAAGTCCAGGGGCCTGTCAGTCTGAATGGTTGTCCTGACCAGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_198129
• Insert Size: 10002 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198129.2
• RefSeq Size: 10666 bp
• RefSeq ORF: 10002 bp
• Locus ID: 3909
• UniProt ID: Q16787
• Cytogenetics: 18q11.2
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• MW: 366.6 kDa
• Gene Summary: The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
  Transcript Variant: This variant (1, also known as b1) represents the longest transcript and encodes the longest isoform (1, also known as alpha3B1). Variants 1 and 3 encode 'b' isoforms.