NDRG2 (NM_201536) Human Untagged Clone

CAT#: SC308044

NDRG2 (untagged)-Human NDRG family member 2 (NDRG2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDRG2
• Synonyms: SYLD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308044 representing NM_201536.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGAGCTGCAGGAGGTGCAGATCACAGAGGAGAAGCCACTGTTGCCAGGACAGACGCCTGAGGCG
  GCCAAGACTCACTCTGTGGAGACACCATACGGCTCTGTCACTTTCACTGTCTATGGCACCCCCAAACCC
  AAACGCCCAGCGATCCTTACCTACCACGATGTGGGACTCAACTATAAATCTTGCTTCCAGCCACTGTTT
  CAGTTCGAGGACATGCAGGAAATCATTCAGAACTTTGTGCGGGTTCATGTGGATGCCCCTGGAATGGAA
  GAGGGAGCCCCTGTGTTCCCTTTGGGATATCAGTACCCATCTCTGGACCAGCTTGCAGACATGATCCCT
  TGCGTCCTGCAGTACCTAAATTTCTCTACAATAATTGGAGTTGGTGTTGGAGCTGGAGCCTACATCCTG
  GCGAGATATGCTCTTAACCACCCGGACACTGTTGAAGGTCTTGTCCTCATCAACATTGATCCCAATGCC
  AAGGGTTGGATGGATTGGGCAGCCCACAAGCTAACAGGCCTCACCTCTTCCATTCCGGAGATGATCCTT
  GGACATCTTTTCAGCCAGGAAGAGCTCTCTGGAAATTCTGAGTTGATACAAAAGTACAGAAATATCATT
  ACACATGCACCCAACCTGGATAACATTGAATTGTACTGGAACAGCTACAACAACCGCCGAGACCTGAAC
  TTTGAGCGTGGAGGTGATATCACCCTCAGGTGTCCTGTGATGCTGGTGGTAGGAGACCAAGCACCTCAT
  GAAGATGCAGTGGTGGAATGTAACTCAAAACTGGACCCCACCCAGACCTCGTTCCTCAAGATGGCTGAC
  TCCGGAGGTCAGCCCCAGCTGACTCAGCCAGGCAAGCTGACCGAGGCCTTCAAGTACTTCCTGCAAGGC
  ATGGGCTACATGGCCTCATCCTGCATGACTCGCCTGTCCCGGTCTCGTACAGCCTCTCTGACCAGTGCA
  GCATCCGTTGATGGCAACCGGTCCCGCTCTCGCACCCTGTCCCAGAGCAGCGAGTCTGGAACTCTTTCT
  TCGGGGCCCCCGGGGCACACCATGGAGGTCTCCTGTTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_201536
• Insert Size: 1074 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_201536.1
• RefSeq Size: 2033 bp
• RefSeq ORF: 1074 bp
• Locus ID: 57447
• UniProt ID: Q9UN36
• Cytogenetics: 14q11.2
• MW: 39.3 kDa
• Gene Summary: This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks an alternate in-frame exon in the coding region, compared to variant 1, resulting in a shorter protein (isoform b) that has a shorter N-terminus, compared to isoform a. Variants 2, 3, 5, 8, 11, and 12 encode the same isoform (b).