KCNQ4 (NM_172163) Human Untagged Clone

CAT#: SC306729

KCNQ4 (untagged)-Human potassium voltage-gated channel, KQT-like subfamily, member 4 (KCNQ4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNQ4
• Synonyms: DFNA2; DFNA2A; KV7.4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_172163 edited
  ATGGCCGAGGCCCCCCCGCGCCGCCTCGGCCTGGGTCCCCCGCCCGGGGACGCCCCCCGC
  GCGGAGCTAGTGGCGCTCACGGCCGTGCAGAGCGAACAGGGCGAGGCGGGCGGGGGCGGC
  TCCCCGCGCCGCCTCGGCCTCCTGGGCAGCCCCCTGCCGCCGGGCGCGCCCCTCCCTGGG
  CCGGGCTCCGGCTCGGGCTCCGCCTGCGGCCAGCGCTCCTCGGCCGCGCACAAGCGCTAC
  CGCCGCCTGCAGAACTGGGTCTACAACGTGCTGGAGCGGCCCCGCGGCTGGGCCTTCGTC
  TACCACGTCTTCATATTTTTGCTGGTCTTCAGCTGCCTGGTGCTGTCTGTGCTGTCCACT
  ATCCAGGAGCACCAGGAACTTGCCAACGAGTGTCTCCTCATCTTGGAATTCGTGATGATC
  GTGGTTTTCGGCTTGGAGTACATCGTCCGGGTCTGGTCCGCCGGATGCTGCTGCCGCTAC
  CGAGGATGGCAGGGTCGCTTCCGCTTTGCCAGAAAGCCCTTCTGTGTCATCGACTTCATC
  GTGTTCGTGGCCTCGGTGGCCGTCATCGCCGCGGGTACCCAGGGCAACATCTTCGCCACG
  TCCGCGCTGCGCAGCATGCGCTTCCTGCAGATCCTGCGCATGGTGCGCATGGACCGCCGC
  GGCGGCACCTGGAAGCTGCTGGGCTCAGTGGTCTACGCGCATAGCAAGGAGCTGATCACC
  GCCTGGTACATCGGGTTCCTGGTGCTCATCTTCGCCTCCTTCCTGGTCTACCTGGCTGAG
  AAGGACGCCAACTCCGACTTCTCCTCCTACGCCGACTCGCTCTGGTGGGGGACGATTACA
  TTGACAACCATCGGCTATGGTGACAAGACACCGCACACATGGCTGGGCAGGGTCCTGGCT
  GCTGGCTTCGCCTTACTGGGCATCTCTTTCTTTGCCCTGCCTGCCGGCATCCTAGGCTCC
  GGCTTTGCCCTGAAGGTCCAGGAGCAGCACCGGCAGAAGCACTTCGAGAAGCGGAGGATG
  CCGGCAGCCAACCTCATCCAGGCTGCCTGGCGCCTGTACTCCACCGATATGAGCCGGGCC
  TACCTGACAGCCACCTGGTACTACTATGACAGTATCCTCCCATCCTTCAGCAGCCGGATG
  GGCATCAAAGACCGCATCCGCATGGGCAGCTCCCAGCGGCGGACGGGTCCTTCCAAGCAG
  CATCTGGCACCTCCAACAATGCCCACCTCCCCAAGCAGCGAGCAGGTGGGTGAGGCCACC
  AGCCCCACCAAGGTGCAAAAGAGCTGGAGCTTCAATGACCGCACCCGCTTCCGGGCATCT
  CTGAGACTCAAACCCCGCACCTCTGCTGAGGATGCCCCCTCAGAGGAAGTAGCAGAGGAG
  AAGAGCTACCAGTGTGAGCTCACGGTGGACGACATCATGCCTGCTGTGAAGACAGTCATC
  CGCTCCATCAGGATTCTCAAGTTCCTGGTGGCCAAAAGGAAATTCAAGGAGACACTGCGA
  CCGTACGACGTGAAGGACGTCATTGAGCAGTACTCAGCAGGCCACCTGGACATGCTGGGC
  CGGATCAAGAGCCTGCAAACTCGGGTGGACCAAATTGTGGGTCGGGGGCCCGGGGACAGG
  AAGGCCCGGGAGAAGGGCGACAAGGGGCCCTCCGACGCGGAGGTGGTGGATGAAATCAGC
  ATGATGGGACGCGTGGTCAAGGTGGAGAAGCAGGTGCAGTCCATCGAGCACAAGCTGGAC
  CTGCTGTTGGGCTTCTATTCGCGCTGCCTGCGCTCTGGCACCTCGGCCAGCCTGGGCGCC
  GTGCAAGTGCCGCTGTTCGACCCCGACATCACCTCCGACTACCACAGCCCTGTGGACCAC
  GAGGACATCTCCGTCTCCGCACAGACGCTCAGCATCTCCCGCTCGGTCAGCACCAACATG
  GACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_172163 unedited
  GTTCAGCATTTGTATACGACTCACTATAGGCGGCCGCGNATTCAGATCTGGTACCGATAT
  CAAGCTTATGGCCGAGGCCCCCCCGCGCCGCCTCGGCCTGGGTCCCCCGCCCGGGGACGC
  CCCCCGCGCGGAGCTAGTGGCGCTCACGGCCGTGCAGAGCGAACAGGGCGAGGCGGGCGG
  GGGCGGCTCCCCGCGCCGCCTCGGCCTCCTGGGCAGCCCCCTGCCGCCGGGCGCGCCCCT
  CCCTGGGCCGGGCTCCGGCTCGGGCTCCGCCTGCGGCCAGCGCTCCTCGGCCGCGCACAA
  GCGCTACCGCCGCCTGCANAACAGNNNNNACAACGNGCNGNNNCNNNNNNNNNNNNNAGA
  CCNNNNNCCACCACGNCCGACCAGAAAAGCNGGACNNCAACNGCCCGGAGCAGACAGAGC
  AGGACACNANCCNNNANCACCANNAACCCCCCCCCCNNNGNCCCCCCCCCCCGGAACCCN
  NGAAGATCGNGGNTTTCGGCTTGGAGTACATCGTCCGGGTCTGGTCCGCCGGATGCTGCT
  GCCGCTACCGAGGATGGCAGGGTCGCTTCCGCTTTGCCNNNNNNNCCNNNNNNNNNNNNN
  NACNNCNNCNNGNNCNTGGCCTCGGTGGCCGTCATCGCCGCGGGTACCCAAGGCAACATC
  TTCGCCACGTCCGCGCTGCGCAGCATGCGCTTCCTGCAGATCCTGCGCATGGTGCGCATG
  GACCGCCGCGGCGGCACCTGGAAGCTGCTGGGCTCAAAGGTCTACCCGCATTGCAAGGAG
  CTGATCACCCGCCTGTACATCGGGTTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_172163 unedited
  CATTGGGGATGGCAACTTCCAGGGCAGGNAAAGCACTGGGGAGGGGTCACAGGNATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTCAGTCC
  ATGTTGGTGCTGACCGAGCGGGAGATGCTGAGCGTCTGTGCGGAGACGGAGATGTCCTCG
  TGGTCCACAGGGCTGTGGTAGTCGGAGGTGATGTCGGGGTCGAACAGCGGCACTTGCACG
  GCGCCCAGGCTGGCCGAGGTGCCAGAGCGCAGGCAGCGCGAATAGAAGCCCAACAGCAGG
  TCCAGCTTGTGCTCGATGGACTGCACCTGCTTCTCCACCTTGACCACNCNTCCCATCATG
  CTNATTTCATCCACCACCTCCNCGTCGGAGGGCCCCTTGTCGCCCTTCTCCCGGGCCTTC
  CTGTCCCCGGGCCCCCGACCCACAATTTGNTCCCCCCNAGTTTGCAGGCTCTTGATCCGG
  CCCAGCATGTCCAGGTGGCCTGCTGAGTACTGCTCAATGACGTCCTTCACGTCGTACGGT
  CGCAGTGTCTCCTTGAATTTCCTTTTGGCCACCAGGAACTTGANAATCCTGNNGGANCNN
  NNNACTGGCTTCACAGCAGGCATGATGTCGTCCACCGTGAGCTCACACTGGTAGCTCTTC
  TCCTCTGCTACTTCCTCTGAGGGGGCATCCTCAGCAGAGGTGCGGGGTTTGAGTCTCAGA
  GATGCCCGGAAGCGGGTGCGGTCATTGAAGCTCCAGCTCTTTTGCACCTTGGTGGGGCTG
  GTGGCCTCACCCACCTGCTCGCTGCTTGGGGAGGT
  
• Restriction Sites: Please inquire
• ACCN: NM_172163
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172163.1, NP_751895.1
• RefSeq Size: 2173 bp
• RefSeq ORF: 1926 bp
• Locus ID: 9132
• UniProt ID: P56696
• Cytogenetics: 1p34.2
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: The protein encoded by this gene forms a potassium channel that is thought to play a critical role in the regulation of neuronal excitability, particularly in sensory cells of the cochlea. The current generated by this channel is inhibited by M1 muscarinic acetylcholine receptors and activated by retigabine, a novel anti-convulsant drug. The encoded protein can form a homomultimeric potassium channel or possibly a heteromultimeric channel in association with the protein encoded by the KCNQ3 gene. Defects in this gene are a cause of nonsyndromic sensorineural deafness type 2 (DFNA2), an autosomal dominant form of progressive hearing loss. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. There are no publicly available transcripts supporting this variant; it is represented based on data in PMID:10025409.