BSND (NM_057176) Human Untagged Clone

CAT#: SC305749

BSND (untagged)-Human Bartter syndrome, infantile, with sensorineural deafness (Barttin) (BSND)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BSND
• Synonyms: BART; DFNB73
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_057176 edited
  GGCTGGGTGTGGGTCCGTTGAAGCGAGCCGCCTCCAGCCCTGTTGAACTGGTGGGCCCAG
  GGACTGGAGCGGGATTGAAAGGGATCTTGCTCTCCCTTGAAGCCTCGAGTTGCAGCGATT
  TCAGTGTCTTCTCTCCCTGTGTAAGCCTGTCTGGGTGTTTAGGCTGAACTACAGCCACCC
  CCTCTCCCGGGGGTGTGCAGGCCAGGGACTGGCCAGGCAGCCATGGCTGACGAGAAGACC
  TTCCGGATCGGCTTCATTGTGCTGGGGCTTTTCCTGCTGGCCCTCGGTACGTTCCTCATG
  AGCCATGATCGGCCCCAGGTCTACGGCACCTTCTATGCCATGGGCAGCGTCATGGTGATC
  GGGGGCATCATCTGGAGCATGTGCCAGTGCTACCCCAAGATCACCTTCGTCCCTGCTGAC
  TCTGACTTTCAAGGCATCCTCTCCCCAAAGGCCATGGGCCTGCTGGAGAATGGGCTTGCT
  GCCGAGATGAAGAGCCCCAGTCCCCAGCCGCCCTATGTAAGGCTGTGGGAGGAAGCCGCC
  TATGACCAGAGCCTGCCTGACTTCAGCCACATCCAGATGAAAGTCATGAGCTACAGTGAG
  GACCACCGCTCCTTGCTGGCCCCTGAGATGGGGCAGCCGAAGCTGGGAACCAGTGATGGA
  GGAGAAGGTGGCCCTGGCGACGTTCAGGCCTGGATGGAGGCTGCCGTGGTCATCCACAAG
  GGCTCAGACGAGAGTGAAGGGGAAAGACGCCTAACTCAGAGCTGGCCCGGCCCCCTGGCC
  TGTCCCCAGGGCCCTGCCCCCTTGGCTTCCTTCCAAGATGACCTGGACATGGACTCCAGT
  GAAGGCAGCAGCCCCAATGCATCTCCACATGACAGGGAGGAAGCTTGTTCCCCACAACAG
  GAACCTCAGGGCTGCAGGTGCCCGCTGGACCGCTTCCAAGACTTTGCCCTGATTGATGCC
  CCAACGTTGGAGGATGAGCCCCAAGAGGGGCAGCAGTGGGAAATAGCCCTGCCCAACAAC
  TGGCAGCGGTACCCAAGGACAAAGGTGGAGGAGAAGGAGGCTTCGGACACAGGTGGGGAG
  GAACCTGAGAAGGAAGAGGAAGACCTGTACTATGGGCTGCCAGATGGAGCCGGGGACCTC
  CTCCCGGACAAGGAGCTGGGTTTTGAGCCTGACACCCAAGGCTGAGATGTTTGTGCTCCG
  TAGCTTCTAGTCTGGAACTGCTGCTGACCCCTGTGTGACATCACAGGGCCTCAGTTTCCC
  TATCTGCAAAATGGGATGATATGGAGGTTCAATGAGATGGTGGCATTTTGAGAATGGTAA
  AGAAATACACAGGGAGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_057176
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_056176.2.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_057176.2, NP_476517.1
• RefSeq Size: 1396 bp
• RefSeq ORF: 963 bp
• Locus ID: 7809
• UniProt ID: Q8WZ55
• Cytogenetics: 1p32.3
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear. Mutations in this gene have been associated with Bartter syndrome with sensorineural deafness. [provided by RefSeq, Jul 2008]