CHREBP (MLXIPL) (NM_032954) Human Untagged Clone

CAT#: SC305526

MLXIPL (untagged)-Human MLX interacting protein-like (MLXIPL), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHREBP
• Synonyms: bHLHd14; CHREBP; MIO; MLX; MONDOB; WBSCR14; WS-bHLH
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC305526 sequence for NM_032954 edited (data generated by NextGen Sequencing)
  
  
  ATGGCCGGCGCGCTGGCAGGTCTGGCCGCGGGCTTGCAGGTCCCGCGGGTCGCGCCCAGCCCAGACTCGG
  ACTCGGACACAGACTCGGAGGACCCGAGTCTCCGGCGCAGCGCGGGCGGCTTGCTCCGCTCGCAGGTCAT
  CCACAGCGGTCACTTCATGGTGTCGTCGCCGCACAGCGACTCGCTGCCCCGGCGGCGCGACCAGGAGGGG
  TCCGTGGGGCCCTCCGACTTCGGGCCGCGCAGTATCGACCCCACACTCACACGCCTCTTCGAGTGCTTGA
  GCCTGGCCTACAGTGGCAAGCTGGTGTCTCCCAAGTGGAAGAATTTCAAAGGCCTCAAGCTGCTCTGCAG
  AGACAAGATCCGCCTGAACAACGCCATCTGGAGGGCCTGGTATATCCAGTATGTGAAGCGGAGGAAGAGC
  CCCGTGTGTGGCTTCGTGACCCCCCTGCAGGGGCCTGAGGCTGATGCGCACCGGAAGCCGGAGGCCGTGG
  TCCTGGAGGGGAACTACTGGAAGCGGCGCATCGAGGTGGTGATGCGGGAATACCACAAGTGGCGCATCTA
  CTACAAGAAGCGGCTCCGTAAGCCCAGCAGGGAAGATGACCTCCTGGCCCCTAAGCAGGCGGAAGGCAGG
  TGGCCGCCGCCGGAGCAATGGTGCAAACAGCTCTTCTCCAGTGTGGTCCCCGTGCTGCTGGGGGACCCAG
  AGGAGGAGCCGGGTGGGCGGCAGCTCCTGGACCTCAATTGCTTTTTGTCCGACATCTCGGACACTCTCTT
  CACCATGACTCAGTCCGGCCCTTCGCCCCTGCAGCTGCCGCCTGAGGATGCCTACGTCGGCAATGCTGAC
  ATGATCCAGCCGGACCTGACGCCACTGCAGCCAAGCCTGGATGACTTCATGGACATCTCAGATTTCTTTA
  CCAACTCCCGCCTCCCACAGCCGCCCATGCCTTCAAACTTCCCAGAGCCCCCCAGCTTCAGCCCCGTGGT
  TGACTCCCTCTTCAGCAGTGGGACCCTGGGCCCAGAGGTGCCCCCGGCTTCCTCGGCCATGACCCACCTC
  TCTGGACACAGCCGTCTGCAGGCTCGGAACAGCTGCCCTGGCCCCTTGGACTCCAGCGCCTTCCTGAGTT
  CTGATTTCCTCCTTCCTGAAGACCCCAAGCCCCGGCTCCCACCCCCTCCTGTACCCCCACCTCTGCTGCA
  TTACCCTCCCCCTGCCAAGGTGCCAGGCCTGGAGCCCTGCCCCCCACCTCCCTTCCCTCCCATGGCACCA
  CCCACTGCTTTGCTGCAGGAAGAGCCTCTCTTCTCTCCCAGGTTTCCCTTCCCCACCGTCCCTCCTGCCC
  CAGGAGTGTCTCCGCTGCCTGCTCCTGCAGCCTTCCCACCCACCCCACAGTCTGTCCCCAGCCCAGCCCC
  CACCCCCTTCCCCATAGAGCTTCTACCCTTGGGGTATTCGGAGCCTGCCTTTGGGCCTTGCTTCTCCATG
  CCCAGAGGCAAGCCCCCCGCCCCATCCCCTAGGGGACAGAAAGCCAGCCCCCCTACCTTAGCCCCTGCCA
  CTGCCAGTCCCCCCACCACTGCGGGGAGCAACAACCCCTGCCTCACACAGCTGCTCACAGCAGCTAAGCC
  GGAGCAAGCCCTGGAGCCACCACTTGTATCCAGCACCCTCCTCCGGTCCCCAGGGTCCCCGCAGGAGACA
  GTCCCTGAATTCCCCTGCACATTCCTTCCCCCGACCCCGGCCCCTACACCGCCCCGGCCACCTCCAGGCC
  CGGCCACATTGGCCCCTTCCAGGCCCCTGCTTGTCCCCAAAGCGGAGCGGCTCTCACCCCCAGCGCCCAG
  CGGCAGTGAACGGCGGCTGTCAGGGGACCTCAGCTCCATGCCAGGCCCTGGGACTCTGAGCGTCCGTGTC
  TCTCCCCCGCAACCCATCCTCAGCCGGGGCCGTCCAGACAGCAACAAGAACCGGCGTATCACACACATCT
  CCGCGGAGCAGAAGCGGCGCTTCAACATCAAGCTGGGGTTTGACACCCTTCATGGGCTCGTGAGCACACT
  CAGTGCCCAGCCCAGCCTCAAGGAGCGTGCGGGCTTGCAGGAGGAGGCCCAGCAGCTGCGGGATGAGATT
  GAGGAGCTCAATGCCGCCATTAACCTGTGCCAGCAGCAGCTGCCCGCCACAGGGGTACCCATCACACACC
  AGCGTTTTGACCAGATGCGAGACATGTTTGATGACTACGTCCGAACCCGTACGCTGCACAACTGGAAGTT
  CTGGGTGTTCAGCATCCTCATCCGGCCTCTGTTTGAGTCCTTCAACGGGATGGTGTCCACGGCAAGTGTG
  CACACCCTCCGCCAGACCTCACTGGCCTGGCTGGACCAGTACTGCTCTCTGCCCGCTCTCCGGCCAACTG
  TCCTGAACTCCCTACGCCAGCTGGGCACATCTACCAGTATCCTGACCGACCCGGGCCGCATCCCTGAGCA
  AGCCACACGGGCAGTCACAGAGGGCACCCTTGGCAAACCTTTATAG
  
  Clone variation with respect to NM_032954.2
  759:g=>a
• Restriction Sites: Please inquire
• ACCN: NM_032954
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032954.2, NP_116572.1
• RefSeq Size: 3225 bp
• RefSeq ORF: 2496 bp
• Locus ID: 51085
• UniProt ID: Q9NP71
• Cytogenetics: 7q11.23
• Gene Summary: This gene encodes a basic helix-loop-helix leucine zipper transcription factor of the Myc/Max/Mad superfamily. This protein forms a heterodimeric complex and binds and activates, in a glucose-dependent manner, carbohydrate response element (ChoRE) motifs in the promoters of triglyceride synthesis genes. The gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (4) uses an alternate in-frame splice site in the coding region, compared to variant 1, resulting in a shorter protein (isoform delta).