BRIP1 (NM_032043) Human Untagged Clone

CAT#: SC305391

BRIP1 (untagged)-Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRIP1
• Synonyms: BACH1; FANCJ; OF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC305391 representing NM_032043.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTTCAATGTGGTCTGAATATACAATTGGTGGGGTGAAGATTTACTTTCCTTATAAAGCTTACCCG
  TCACAGCTTGCTATGATGAATTCTATTCTCAGAGGATTAAACAGCAAGCAACATTGTTTGTTGGAGAGT
  CCCACAGGAAGTGGAAAAAGCTTAGCCTTACTTTGTTCTGCTTTAGCATGGCAACAATCTCTTAGTGGG
  AAACCAGCAGATGAGGGCGTAAGTGAAAAAGCTGAAGTACAATTGTCATGTTGTTGTGCATGCCATTCA
  AAGGATTTTACAAACAATGACATGAACCAAGGAACTTCACGTCATTTCAACTATCCAAGCACACCACCT
  TCTGAAAGAAATGGCACTTCATCAACTTGTCAAGACTCCCCTGAAAAAACCACTCTGGCTGCAAAGTTA
  TCTGCTAAGAAACAGGCATCCATATACAGAGATGAAAATGATGATTTTCAAGTAGAGAAGAAAAGAATT
  CGACCCTTAGAAACTACACAGCAGATTAGAAAACGTCATTGCTTTGGAACAGAAGTACACAATTTGGAT
  GCAAAAGTTGATTCAGGAAAGACTGTAAAACTCAACTCTCCACTGGAAAAGATAAACTCCTTTTCGCCA
  CAGAAACCCCCTGGCCACTGTTCTAGGTGCTGTTGTTCTACTAAACAAGGAAACAGTCAAGAGTCATCG
  AATACCATTAAGAAGGATCATACAGGGAAATCCAAGATACCCAAAATATATTTTGGGACACGCACACAC
  AAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACGGCATATTCAGGGGTTCCAATGACTATTCTT
  TCCAGCAGGGATCATACTTGTGTCCATCCTGAGGTAGTCGGTAACTTCAACAGAAATGAGAAGTGCATG
  GAATTGCTAGATGGGAAAAACGGAAAATCCTGCTATTTTTATCATGGAGTTCATAAAATTAGTGATCAG
  CACACATTACAGACTTTCCAAGGGATGTGCAAAGCCTGGGATATAGAAGAACTTGTCAGCCTGGGGAAG
  AAACTAAAGGCCTGTCCATATTACACAGCCCGAGAACTAATACAAGATGCTGACATCATATTTTGTCCC
  TACAACTATCTTCTAGATGCACAAATAAGGGAAAGTATGGATTTAAATCTGAAAGAACAGGTTGTCATT
  TTAGATGAAGCTCATAACATCGAGGACTGTGCTCGGGAATCAGCAAGTTACAGTGTAACAGAAGTTCAG
  CTTCGGTTTGCTCGGGATGAACTAGATAGTATGGTCAACAATAATATAAGGAAGAAAGATCATGAACCC
  CTACGAGCTGTGTGCTGTAGCCTCATTAATTGGTTAGAAGCAAACGCTGAATATCTTGTAGAAAGAGAT
  TATGAATCAGCTTGTAAAATATGGAGTGGAAATGAAATGCTCTTAACTTTACACAAAATGGGTATCACC
  ACTGCTACTTTTCCCATTTTGCAGGGACATTTTTCTGCTGTTCTTCAAAAAGAGGAAAAAATCTCACCA
  ATTTATGGTAAAGAGGAGGCAAGAGAAGTACCTGTTATTAGTGCATCAACTCAAATAATGCTTAAAGGA
  CTTTTTATGGTACTTGACTATCTTTTTAGGCAAAATAGCAGATTTGCAGATGATTATAAAATTGCGATT
  CAACAGACTTACTCCTGGACAAATCAGATTGATATTTCAGACAAAAATGGGTTGTTGGTTCTACCAAAA
  AATAAGAAACGTTCACGACAGAAAACTGCAGTTCATGTGCTAAACTTTTGGTGCTTAAATCCAGCTGTG
  GCCTTTTCAGATATTAATGGCAAAGTTCAGACCATTGTTTTGACATCTGGTACATTATCACCAATGAAA
  TCCTTTTCGTCAGAACTTGGTGTTACATTTACTATCCAGCTGGAGGCTAATCATATCATTAAAAATTCA
  CAGGTTTGGGTTGGTACCATTGGGTCAGGCCCCAAGGGTCGGAATCTCTGTGCTACCTTCCAGAATACT
  GAAACATTTGAGTTCCAAGATGAAGTGGGAGCACTTTTGTTATCTGTGTGCCAGACTGTGAGCCAAGGA
  ATTTTGTGTTTCTTGCCATCTTACAAGTTATTAGAAAAATTAAAAGAACGTTGGCTCTCTACTGGTTTA
  TGGCATAATCTGGAGTTGGTGAAGACAGTCATTGTAGAACCACAGGGAGGAGAAAAAACAAATTTTGAT
  GAATTACTGCAGGTGTACTATGACGCAATCAAATACAAAGGAGAGAAAGATGGAGCTCTCCTGGTAGCA
  GTTTGTCGTGGTAAAGTGAGTGAGGGTCTGGATTTCTCAGATGACAATGCCCGTGCTGTCATAACAATA
  GGAATTCCTTTTCCAAATGTGAAAGATCTACAGGTTGAACTAAAACGACAATACAATGACCACCATTCA
  AAATTGAGAGGTCTTCTACCTGGCCGTCAGTGGTATGAAATTCAAGCATACAGGGCCTTAAACCAGGCC
  CTTGGTAGATGTATTAGACACAGAAATGATTGGGGAGCTCTTATTCTAGTGGATGATCGCTTTAGGAAT
  AACCCAAGTCGCTATATATCTGGACTTTCTAAATGGGTACGGCAGCAGATTCAGCACCATTCAACCTTT
  GAAAGTGCACTGGAATCCTTGGCTGAATTTTCCAAAAAGCATCAAAAAGTTCTTAATGTATCCATAAAG
  GACAGAACCAATATACAGGACAATGAGTCTACACTTGAAGTGACCTCTTTAAAGTACAGTACCTCACCT
  TATTTACTGGAAGCAGCAAGTCATCTATCACCAGAAAATTTTGTGGAAGATGAAGCAAAGATATGTGTC
  CAGGAACTACAGTGTCCTAAAATTATTACCAAAAATTCACCTCTACCAAGTAGCATTATCTCCAGAAAG
  GAGAAAAATGATCCAGTATTCCTGGAAGAAGCAGGGAAAGCAGAAAAAATTGTGATTTCCAGATCCACA
  AGCCCAACTTTCAACAAACAAACAAAGAGAGTTAGCTGGTCAAGCTTTAATTCTTTGGGACAGTATTTT
  ACTGGTAAAATACCGAAGGCAACACCTGAGCTCGGGTCATCAGAGAATAGTGCCTCTAGTCCTCCCCGT
  TTCAAAACAGAGAAGATGGAAAGTAAAACTGTTTTGCCCTTCACTGATAAATGTGAATCCTCAAATCTG
  ACAGTAAACACATCGTTTGGATCATGCCCTCAATCAGAAACCATTATTTCATCATTAAAGATTGATGCC
  ACCCTTACTAGAAAAAATCATTCTGAACATCCGCTCTGTTCTGAAGAAGCCCTGGATCCAGACATTGAA
  TTGTCTCTAGTAAGTGAAGAAGATAAACAGTCCACTTCAAATAGAGATTTTGAAACAGAAGCAGAAGAT
  GAATCTATCTATTTTACACCTGAACTTTATGATCCTGAAGATACAGATGAAGAAAAAAATGACCTAGCT
  GAAACTGATAGAGGAAATAGATTGGCTAACAATTCAGATTGCATTTTAGCTAAAGACCTTTTTGAAATT
  AGAACTATAAAAGAAGTAGATTCAGCCAGAGAAGTGAAAGCTGAGGATTGCATAGATACAAAGTTGAAT
  GGAATTCTGCATATTGAAGAAAGTAAAATTGATGACATTGATGGTAATGTAAAAACAACTTGGATAAAT
  GAACTGGAACTGGGAAAAACTCATGAAATAGAAATAAAGAACTTTAAACCATCTCCTTCCAAAAATAAA
  GGCATGTTTCCTGGTTTTAAGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_032043
• Insert Size: 3750 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032043.2
• RefSeq Size: 8166 bp
• RefSeq ORF: 3750 bp
• Locus ID: 83990
• UniProt ID: Q9BX63
• Cytogenetics: 17q23.2
• Protein Families: Druggable Genome
• MW: 140.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq, Jul 2008]