BRIP1 (NM_032043) Human Tagged ORF Clone

CAT#: RC224085

BRIP1 (Myc-DDK-tagged)-Human BRCA1 interacting protein C-terminal helicase 1 (BRIP1)

Product images

Western blot validation of overexpression lysate (Cat# LY410352) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224085 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Product Name: BRIP1 (NM_032043) Human Tagged ORF Clone
• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BRIP1
• Synonyms: BACH1; FANCJ; OF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224085 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTTCAATGTGGTCTGAATATACAATTGGTGGGGTGAAGATTTACTTTCCTTATAAAGCTTACCCGT
  CACAGCTTGCTATGATGAATTCTATTCTCAGAGGATTAAACAGCAAGCAACATTGTTTGTTGGAGAGTCC
  CACAGGAAGTGGAAAAAGCTTAGCCTTACTTTGTTCTGCTTTAGCATGGCAACAATCTCTTAGTGGGAAA
  CCAGCAGATGAGGGCGTAAGTGAAAAAGCTGAAGTACAATTGTCATGTTGTTGTGCATGCCATTCAAAGG
  ATTTTACAAACAATGACATGAACCAAGGAACTTCACGTCATTTCAACTATCCAAGCACACCACCTTCTGA
  AAGAAATGGCACTTCATCAACTTGTCAAGACTCCCCTGAAAAAACCACTCTGGCTGCAAAGTTATCTGCT
  AAGAAACAGGCATCCATATACAGAGATGAAAATGATGATTTTCAAGTAGAGAAGAAAAGAATTCGACCCT
  TAGAAACTACACAGCAGATTAGAAAACGTCATTGCTTTGGAACAGAAGTACACAATTTGGATGCAAAAGT
  TGATTCAGGAAAGACTGTAAAACTCAACTCTCCACTGGAAAAGATAAACTCCTTTTCGCCACAGAAACCC
  CCTGGCCACTGTTCTAGGTGCTGTTGTTCTACTAAACAAGGAAACAGTCAAGAGTCATCGAATACCATTA
  AGAAGGATCATACAGGGAAATCCAAGATACCCAAAATATATTTTGGGACACGCACACACAAGCAGATTGC
  TCAGATTACTAGAGAGCTCCGGAGGACGGCATATTCAGGGGTTCCAATGACTATTCTTTCCAGCAGGGAT
  CATACTTGTGTCCATCCTGAGGTAGTCGGTAACTTCAACAGAAATGAGAAGTGCATGGAATTGCTAGATG
  GGAAAAACGGAAAATCCTGCTATTTTTATCATGGAGTTCATAAAATTAGTGATCAGCACACATTACAGAC
  TTTCCAAGGGATGTGCAAAGCCTGGGATATAGAAGAACTTGTCAGCCTGGGGAAGAAACTAAAGGCCTGT
  CCATATTACACAGCCCGAGAACTAATACAAGATGCTGACATCATATTTTGTCCCTACAACTATCTTCTAG
  ATGCACAAATAAGGGAAAGTATGGATTTAAATCTGAAAGAACAGGTTGTCATTTTAGATGAAGCTCATAA
  CATCGAGGACTGTGCTCGGGAATCAGCAAGTTACAGTGTAACAGAAGTTCAGCTTCGGTTTGCTCGGGAT
  GAACTAGATAGTATGGTCAACAATAATATAAGGAAGAAAGATCATGAACCCCTACGAGCTGTGTGCTGTA
  GCCTCATTAATTGGTTAGAAGCAAACGCTGAATATCTTGTAGAAAGAGATTATGAATCAGCTTGTAAAAT
  ATGGAGTGGAAATGAAATGCTCTTAACTTTACACAAAATGGGTATCACCACTGCTACTTTTCCCATTTTG
  CAGGGACATTTTTCTGCTGTTCTTCAAAAAGAGGAAAAAATCTCACCAATTTATGGTAAAGAGGAGGCAA
  GAGAAGTACCTGTTATTAGTGCATCAACTCAAATAATGCTTAAAGGACTTTTTATGGTACTTGACTATCT
  TTTTAGGCAAAATAGCAGATTTGCAGATGATTATAAAATTGCGATTCAACAGACTTACTCCTGGACAAAT
  CAGATTGATATTTCAGACAAAAATGGGTTGTTGGTTCTACCAAAAAATAAGAAACGTTCACGACAGAAAA
  CTGCAGTTCATGTGCTAAACTTTTGGTGCTTAAATCCAGCTGTGGCCTTTTCAGATATTAATGGCAAAGT
  TCAGACCATTGTTTTGACATCTGGTACATTATCACCAATGAAATCCTTTTCGTCAGAACTTGGTGTTACA
  TTTACTATCCAGCTGGAGGCTAATCATATCATTAAAAATTCACAGGTTTGGGTTGGTACCATTGGGTCAG
  GCCCCAAGGGTCGGAATCTCTGTGCTACCTTCCAGAATACTGAAACATTTGAGTTCCAAGATGAAGTGGG
  AGCACTTTTGTTATCTGTGTGCCAGACTGTGAGCCAAGGAATTTTGTGTTTCTTGCCATCTTACAAGTTA
  TTAGAAAAATTAAAAGAACGTTGGCTCTCTACTGGTTTATGGCATAATCTGGAGTTGGTGAAGACAGTCA
  TTGTAGAACCACAGGGAGGAGAAAAAACAAATTTTGATGAATTACTGCAGGTGTACTATGACGCAATCAA
  ATACAAAGGAGAGAAAGATGGAGCTCTCCTGGTAGCAGTTTGTCGTGGTAAAGTGAGTGAGGGTCTGGAT
  TTCTCAGATGACAATGCCCGTGCTGTCATAACAATAGGAATTCCTTTTCCAAATGTGAAAGATCTACAGG
  TTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTCTTCTACCTGGCCGTCAGTGGTA
  TGAAATTCAAGCATACAGGGCCTTAAACCAGGCCCTTGGTAGATGTATTAGACACAGAAATGATTGGGGA
  GCTCTTATTCTAGTGGATGATCGCTTTAGGAATAACCCAAGTCGCTATATATCTGGACTTTCTAAATGGG
  TACGGCAGCAGATTCAGCACCATTCAACCTTTGAAAGTGCACTGGAGTCCTTGGCTGAATTTTCCAAAAA
  GCATCAAAAAGTTCTTAATGTATCCATAAAGGACAGAACCAATATACAGGACAATGAGTCTACACTTGAA
  GTGACCTCTTTAAAGTACAGTACCCCACCTTATTTACTGGAAGCAGCAAGTCATCTATCACCAGAAAATT
  TTGTGGAAGATGAAGCAAAGATATGTGTCCAGGAACTACAGTGTCCTAAAATTATTACCAAAAATTCACC
  TCTACCAAGTAGCATTATCTCCAGAAAGGAGAAAAATGATCCAGTATTCCTGGAAGAAGCAGGGAAAGCA
  GAAAAAATTGTGATTTCCAGATCCACAAGCCCAACTTTCAACAAACAAACAAAGAGAGTTAGCTGGTCAA
  GCTTTAATTCTTTGGGACAGTATTTTACTGGTAAAATACCGAAGGCAACACCTGAGCTCGGGTCATCAGA
  GAATAGTGCCTCTAGTCCTCCCCGTTTCAAAACAGAGAAGATGGAAAGTAAAACTGTTTTGCCCTTCACT
  GATAAATGTGAATCCTCAAATCTGACAGTAAACACATCGTTTGGATCATGCCCTCAATCAGAAACCATTA
  TTTCATCATTAAAGATTGATGCCACCCTTACTAGAAAAAATCATTCTGAACATCCGCTCTGTTCTGAAGA
  AGCCCTGGATCCAGACATTGAATTGTCTCTAGTAAGTGAAGAAGATAAACAGTCCACTTCAAATAGAGAT
  TTTGAAACAGAAGCAGAAGATGAATCTATCTATTTTACACCTGAACTTTACGATCCTGAAGATACAGATG
  AAGAAAAAAATGACCTAGCTGAAACTGATAGAGGAAATAGATTGGCTAACAATTCAGATTGCATTTTAGC
  TAAAGACCTTTTTGAAATTAGAACTATAAAAGAAGTAGATTCAGCCAGAGAAGTGAAAGCTGAGGATTGC
  ATAGATACAAAGTTGAATGGAATTCTGCATATTGAAGAAAGTAAAATTGATGACATTGATGGTAATGTAA
  AAACAACTTGGATAAATGAACTGGAACTGGGAAAAACTCATGAAATAGAAATAAAGAACTTTAAACCATC
  TCCTTCCAAAAATAAAGGCATGTTTCCTGGTTTTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224085 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSGK
  PADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSA
  KKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKP
  PGHCSRCCCSTKQGNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRD
  HTCVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVSLGKKLKAC
  PYYTARELIQDADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIEDCARESASYSVTEVQLRFARD
  ELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVERDYESACKIWSGNEMLLTLHKMGITTATFPIL
  QGHFSAVLQKEEKISPIYGKEEAREVPVISASTQIMLKGLFMVLDYLFRQNSRFADDYKIAIQQTYSWTN
  QIDISDKNGLLVLPKNKKRSRQKTAVHVLNFWCLNPAVAFSDINGKVQTIVLTSGTLSPMKSFSSELGVT
  FTIQLEANHIIKNSQVWVGTIGSGPKGRNLCATFQNTETFEFQDEVGALLLSVCQTVSQGILCFLPSYKL
  LEKLKERWLSTGLWHNLELVKTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDGALLVAVCRGKVSEGLD
  FSDDNARAVITIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHRNDWG
  ALILVDDRFRNNPSRYISGLSKWVRQQIQHHSTFESALESLAEFSKKHQKVLNVSIKDRTNIQDNESTLE
  VTSLKYSTPPYLLEAASHLSPENFVEDEAKICVQELQCPKIITKNSPLPSSIISRKEKNDPVFLEEAGKA
  EKIVISRSTSPTFNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFT
  DKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRD
  FETEAEDESIYFTPELYDPEDTDEEKNDLAETDRGNRLANNSDCILAKDLFEIRTIKEVDSAREVKAEDC
  IDTKLNGILHIEESKIDDIDGNVKTTWINELELGKTHEIEIKNFKPSPSKNKGMFPGFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032043
• ORF Size: 3747 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032043.1, NP_114432.1
• RefSeq Size: 8166 bp
• RefSeq ORF: 3750 bp
• Locus ID: 83990
• Cytogenetics: 17q23.2
• Protein Families: Druggable Genome
• MW: 140.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq, Jul 2008]