OPLAH (NM_017570) Human Untagged Clone

CAT#: SC304485

OPLAH (untagged)-Human 5-oxoprolinase (ATP-hydrolysing) (OPLAH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: OPLAH
• Synonyms: 5-Opase; OPLA; OPLAHD
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017570 edited
  GTGAAGAGCTCCTGTGGGACTCCAGCCCCACCACCATGGGCAGCCCCGAGGGCCGCTTCC
  ACTTTGCCATCGACCGTGGGGGTACCTTCACAGACGTCTTTGCCCAGTGCCCAGGGGGGC
  ACGTGCGGGTCTTAAAACTGCTCTCAGAGGACCCTGCCAACTATGCGGACGCGCCAACCG
  AAGGCATCCGCCGCATCCTGGAGCAGGAGGCCGGCATGCTCCTGCCCCGGGACCAGCCGC
  TGGACTCCAGTCATATCGCCAGCATCCGCATGGGCACCACAGTGGCCACCAACGCACTGC
  TGGAGCGGAAGGGGGAGCGGGTGGCGCTGCTGGTGACACGTGGCTTCCGAGACCTGCTGC
  ACATTGGCACCCAAGCCCGTGGGGACCTCTTTGACCTGGCCGTGCCCATGCCTGAGGTGC
  TGTATGAAGAGGTGCTGGAGGTGGACGAACGCGTGGTGCTGCACCGTGGAGAGGCGGGCA
  CCGGGACGCCTGTGAAAGGCCGCACGGGGGACCTGCTGGAAGTGCAGCAGCCTGTGGACC
  TGGGGGCCCTGCGTGGGAAGCTGGAGGGGCTGCTATCTCGAGGCATCCGCAGCCTGGCTG
  TGGTGCTCATGCACTCGTACACGTGGGCCCAGCATGAGCAGCAGGTGGGTGTGCTGGCCC
  GGGAGCTGGGCTTCACGCACGTGTCACTGTCCTCGGAGGCCATGCCCATGGTGCGCATCG
  TCCCTCGGGGGCACACGGCCTGTGCCGACGCCTACCTCACGCCCGCCATCCAGCGCTACG
  TGCAGGGCTTCTGCCGTGGCTTCCAGGGCCAACTCAAGGATGTGCAGGTGTTGTTCATGC
  GCTCCGATGGCGGCCTGGCGCCCATGGACACCTTCAGCGGCTCCAGTGCTGTGCTCTCGG
  GCCCGGCCGGCGGCGTGGTGGGCTACTCAGCCACCACCTACCAGCAGGAGGGTGGCCAGC
  CTGTCATCGGCTTTGACATGGGAGGCACGTCCACGGATGTGAGCCGCTATGCTGGGGAAT
  TCGAGCACGTCTTCGAGGCCAGCACAGCTGGCGTCACCCTCCAGGCCCCGCAGCTGGACA
  TCAACACCGTGGCAGCGGGAGGGGGTTCCCGCCTCTTCTTCAGGTCTGGCCTCTTTGTGG
  TTGGGCCCGAGTCAGCAGGAGCCCACCCAGGACCCGCCTGCTACCGCAAAGGGGGCCCTG
  TGACAGTGACGGATGCTAATCTGGTCCTGGGTCGCCTGCTGCCTGCCTCCTTCCCCTGCA
  TTTTTGGGCCGGGAGAGAACCAACCACTTTCCCCTGAGGCCTCCCGCAAAGCCCTGGAGG
  CTGTGGCCACTGAGGTCAACAGCTTCCTGACCAACGGGCCCTGCCCGGCCTCCCCGCTGA
  GCCTGGAGGAGGTGGCCATGGGGTTCGTGCGCGTGGCCAACGAGGCCATGTGCCGGCCCA
  TCCGTGCACTCACGCAGGCAAGAGGCCATGACCCCTCAGCCCATGTGCTGGCCTGCTTTG
  GGGGAGCTGGTGGGCAGCATGCATGTGCCATCGCCCGGGCCCTGGGCATGGACACGGTGC
  ACATCCACAGGCACAGTGGGCTGCTGTCGGCCCTGGGGCTGGCCCTGGCTGACGTGGTGC
  ATGAGGCACAGGAACCCTGCTCCCTGCTCTACGCGCCTGAGACCTTCGTGCAGCTGGACC
  AGAGGCTGAGCCGCCTGGAGGAGCAGTGTGTGGATGCTCTGCAGGCCCAGGGCTTCCCCA
  GGTCCCAGATCAGCACTGAGAGCTTCCTGCACCTGCGCTACCAGGGCACGGACTGTGCTC
  TGATGGTGTCTGCCCACCAGCACCCAGCCACAGCCCGCTCGCCCCGTGCGGGGGACTTCG
  GGGCAGCCTTTGTGGAGCGGTACATGAGGGAGTTTGGCTTTGTCATACCTGAGCGGCCGG
  TGGTCGTGGACGATGTGCGAGTGCGGGGCACCGGCCGCAGTGGTCTTCGCCTCGAGGATG
  CCCCCAAAGCCCAGACCGGGCCTCCCCGGGTGGACAAGATGACCCAGTGCTACTTTGAGG
  GGGGCTACCAGGAGACCCCTGTGTACCTGCTGGCAGAGCTGGGCTATGGGCACAAGCTCC
  ATGGGCCCTGCCTCATCATCGACAGTAACAGCACCATCCTGGTGGAGCCAGGTTGCCAGG
  CAGAGGTGACCAAGACAGGGGACATCTGCATCTCCGTGGGGGCCGAAGTCCCCGGCACAG
  TGGGCCCCCAGCTGGACCCTATCCAGCTGTCCATCTTCTCACACCGCTTCATGAGCATTG
  CTGAGCAGATGGGCCGCATCCTGCAGCGCACAGCCATCTCCACCAACATCAAGGAGCGTC
  TGGACTTCTCCTGTGCCCTCTTTGGGCCCGATGGGGGGCTGGTGTCCAATGCCCCCCACA
  TCCCTGTGCACCTGGGTGCCATGCAGGAGACGGTGCAGTTCCAGATTCAGCACCTGGGGG
  CCGATCTCCACCCTGGCGACGTGCTACTGAGCAACCATCCCAGTGCCGGGGGCAGCCACC
  TGCCAGACCTGACTGTTATCACACCGGTGTTTTGGCCGGGTCAGACGCGGCCTGTGTTCT
  ATGTGGCCAGCCGAGGGCACCACGCAGACATCGGGGGCATCACACCAGGCTCCATGCCCC
  CCCACTCCACCATGCTGCAACAGGAGGGTGCCGTCTTTCTGTCCTTCAAACTTGTCCAGG
  GGGGCGTCTTCCAGGAGGAGGCGGTGACGGAGGCCCTGCGGGCGCCAGGCAAGGTCCCCA
  ACTGCAGCGGAACCAGAAACCTGCACGACAACCTGTCGGACCTCCGTGCCCAGGTGGCAG
  CCAACCAGAAGGGCATCCAGCTGGTGGGGGAGCTCATTGGGCAGTACGGCCTGGACGTGG
  TGCAGGCCTACATGGGCCATATTCAGGCAAACGCTGAGCTGGCCGTGCGAGACATGTTGC
  GTGCCTTTGGAACCTCCCGGCAGGCCCGGGGCCTGCCCCTGGAGGTGTCCTCGGAAGACC
  ACATGGACGACGGTTCCCCCATCCGCCTCCGTGTGCAGATCAGCCTGAGTCAGGGCAGCG
  CTGTGTTTGACTTCAGCGGCACTGGGCCGGAGGTGTTTGGTAATCTCAACGCACCGCGGG
  CCGTAACCCTGTCCGCCCTCATCTACTGCCTGCGCTGTCTGGTGGGCCGCGACATCCCAC
  TCAACCAGGGCTGCCTGGCGCCAGTGCGCGTGGTCATTCCCCGAGGCTCCATCCTGGACC
  CGTCGCCCGAGGCGGCGGTGGTGGGCGGCAACGTGCTCACGTCGCAGCGCGTGGTGGATG
  TCATCCTGGGGGCCTTTGGGGCCTGCGCCGCCTCCCAGGGCTGCATGAACAACGTGACCC
  TGGGCAACGCCCACATGGGCTACTACGAGACGGTGGCGGGCGGCGCGGGCGCGGGTCCCA
  GCTGGCACGGGCGCAGCGGTGTGCACAGCCACATGACCAACACACGCATCACCGACCCTG
  AGATCCTGGAGAGCCGGTACCCGGTCATCCTGCGCCGCTTCGAGCTGCGGCGGGGCTCGG
  GGGGCAGAGGCCGCTTCCGAGGCGGCGACGGCGTCACCCGCGAGCTGCTCTTTCGTGAGG
  AGGCGCTGCTGTCAGTGCTGACCGAGCGCCGCGCCTTCCGGCCATACGGGCTCCACGGGG
  GCGAGCCTGGCGCCCGCGGCCTAAACCTGCTGATCCGCAAAAACGGCCGGACGGTGAATC
  TGGGCGGCAAGACGTCGGTGACCGTGTACCCCGGGGATGTGTTCTGTCTCCACACGCCCG
  GCGGCGGTGGCTATGGGGACCCGGAGGACCCCGCCCCACCGCCGGGGTCGCCCCCGCAAG
  CACTGGCCTTTCCCGAGCACGGCAGCGTCTATGAGTATCGCCGGGCCCAGGAGGCCGTGT
  GAGGATCCCGCAATAAAGATGCCTTAAGTCTCCCGGTTCTGGG
  
• Restriction Sites: Please inquire
• ACCN: NM_017570
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_017570.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017570.1, NP_060040.1
• RefSeq Size: 3944 bp
• RefSeq ORF: 3867 bp
• Locus ID: 26873
• UniProt ID: O14841
• Cytogenetics: 8q24.3
• Protein Pathways: Glutathione metabolism
• Gene Summary: The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD). [provided by RefSeq, Jun 2012]