OPLAH (NM_017570) Human Tagged ORF Clone

CAT#: RG219080

OPLAH (tGFP-tagged) - Human 5-oxoprolinase (ATP-hydrolysing) (OPLAH)

Product Images

Circular map for RG219080

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OPLAH
• Synonyms: 5-Opase; OPLA; OPLAHD
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG219080 representing NM_017570
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAGCCCCGAGGGCCGCTTCCACTTTGCCATCGACCGTGGGGGTACCTTCACAGACGTCTTTGCCC
  AGTGCCCAGGGGGGCACGTGCGGGTCTTAAAACTGCTCTCAGAGGACCCTGCCAACTATGCGGACGCGCC
  AACCGAAGGCATCCGCCGCATCCTGGAGCAGGAGGCCGGCATGCTCCTGCCCCGGGACCAGCCGCTGGAC
  TCCAGTCATATCGCCAGCATCCGCATGGGCACCACAGTGGCCACCAACGCACTGCTGGAGCGGAAGGGGG
  AGCGGGTGGCGCTGCTGGTGACACGTGGCTTCCGAGACCTGCTGCACATTGGCACCCAAGCCCGTGGGGA
  CCTCTTTGACCTGGCCGTGCCCATGCCTGAGGTGCTGTATGAAGAGGTGCTGGAGGTGGACGAACGCGTG
  GTGCTGCACCGTGGAGAGGCGGGCACCGGGACGCCTGTGAAAGGCCGCACGGGGGACCTGCTGGAAGTGC
  AGCAGCCTGTGGACCTGGGGGCCCTGCGTGGGAAGCTGGAGGGGCTGCTATCTCGAGGCATCCGCAGCCT
  GGCTGTGGTGCTCATGCACTCGTACACGTGGGCCCAGCATGAGCAGCAGGTGGGTGTGCTGGCCCGGGAG
  CTGGGCTTCACGCACGTGTCACTGTCCTCGGAGGCCATGCCCATGGTGCGCATCGTCCCTCGGGGGCACA
  CGGCCTGTGCCGACGCCTACCTCACGCCCGCCATCCAGCGCTACGTGCAGGGCTTCTGCCGTGGCTTCCA
  GGGCCAACTCAAGGATGTGCAGGTGTTGTTCATGCGCTCCGATGGCGGCCTGGCGCCCATGGACACCTTC
  AGCGGCTCCAGTGCTGTGCTCTCGGGCCCGGCCGGCGGCGTGGTGGGCTACTCAGCCACCACCTACCAGC
  AGGAGGGTGGCCAGCCTGTCATCGGCTTTGACATGGGAGGCACGTCCACGGATGTGAGCCGCTATGCTGG
  GGAATTCGAGCACGTCTTCGAGGCCAGCACAGCTGGCGTCACCCTCCAGGCCCCGCAGCTGGACATCAAC
  ACCGTGGCAGCGGGAGGGGGTTCCCGCCTCTTCTTCAGGTCTGGCCTCTTTGTGGTTGGGCCCGAGTCAG
  CAGGAGCCCACCCAGGACCCGCCTGCTACCGCAAAGGGGGCCCTGTGACAGTGACGGATGCTAATCTGGT
  CCTGGGTCGCCTGCTGCCTGCCTCCTTCCCCTGCATTTTTGGGCCGGGAGAGAACCAACCACTTTCCCCT
  GAGGCCTCCCGCAAAGCCCTGGAGGCTGTGGCCACTGAGGTCAACAGCTTCCTGACCAACGGGCCCTGCC
  CGGCCTCCCCGCTGAGCCTGGAGGAGGTGGCCATGGGGTTCGTGCGCGTGGCCAACGAGGCCATGTGCCG
  GCCCATCCGTGCACTCACGCAGGCAAGAGGCCATGACCCCTCAGCCCATGTGCTGGCCTGCTTTGGGGGA
  GCTGGTGGGCAGCATGCATGTGCCATCGCCCGGGCCCTGGGCATGGACACGGTGCACATCCACAGGCACA
  GTGGGCTGCTGTCGGCCCTGGGGCTGGCCCTGGCTGACGTGGTGCATGAGGCACAGGAACCCTGCTCCCT
  GCTCTACGCGCCTGAGACCTTCGTGCAGCTGGACCAGAGGCTGAGCCGCCTGGAGGAGCAGTGTGTGGAT
  GCTCTGCAGGCCCAGGGCTTCCCCAGGTCCCAGATCAGCACTGAGAGCTTCCTGCACCTGCGCTACCAGG
  GCACGGACTGTGCTCTGATGGTGTCTGCCCACCAGCACCCAGCCACAGCCCGCTCGCCCCGTGCGGGGGA
  CTTCGGGGCAGCCTTTGTGGAGCGGTACATGAGGGAGTTTGGCTTTGTCATACCTGAGCGGCCGGTGGTC
  GTGGACGATGTGCGAGTGCGGGGCACCGGCCGCAGTGGTCTTCGCCTCGAGGATGCCCCCAAAGCCCAGA
  CCGGGCCTCCCCGGGTGGACAAGATGACCCAGTGCTACTTTGAGGGGGGCTACCAGGAGACCCCTGTGTA
  CCTGCTGGCAGAGCTGGGCTATGGGCACAAGCTCCATGGGCCCTGCCTCATCATCGACAGTAACAGCACC
  ATCCTGGTGGAGCCAGGTTGCCAGGCAGAGGTGACCAAGACAGGGGACATCTGCATCTCCGTGGGGGCCG
  AAGTCCCCGGCACAGTGGGCCCCCAGCTGGACCCTATCCAGCTGTCCATCTTCTCACACCGCTTCATGAG
  CATTGCTGAGCAGATGGGCCGCATCCTGCAGCGCACAGCCATCTCCACCAACATCAAGGAGCGTCTGGAC
  TTCTCCTGTGCCCTCTTTGGGCCCGATGGGGGGCTGGTGTCCAATGCCCCCCACATCCCTGTGCACCTGG
  GTGCCATGCAGGAGACGGTGCAGTTCCAGATTCAGCACCTGGGGGCCGATCTCCACCCTGGCGACGTGCT
  ACTGAGCAACCATCCCAGTGCCGGGGGCAGCCACCTGCCAGACCTGACTGTTATCACACCGGTGTTTTGG
  CCGGGTCAGACGCGGCCTGTGTTCTATGTGGCCAGCCGAGGGCACCACGCAGACATCGGGGGCATCACAC
  CAGGCTCCATGCCCCCCCACTCCACCATGCTGCAACAGGAGGGTGCCGTCTTTCTGTCCTTCAAACTTGT
  CCAGGGGGGCGTCTTCCAGGAGGAGGCGGTGACGGAGGCCCTGCGGGCGCCAGGCAAGGTCCCCAACTGC
  AGCGGAACCAGAAACCTGCACGACAACCTGTCGGACCTCCGTGCCCAGGTGGCAGCCAACCAGAAGGGCA
  TCCAGCTGGTGGGGGAGCTCATTGGGCAGTACGGCCTGGACGTGGTGCAGGCCTACATGGGCCATATTCA
  GGCAAACGCTGAGCTGGCCGTGCGAGACATGTTGCGTGCCTTTGGAACCTCCCGGCAGGCCCGGGGCCTG
  CCCCTGGAGGTGTCCTCGGAAGACCACATGGACGACGGTTCCCCCATCCGCCTCCGTGTGCAGATCAGCC
  TGAGTCAGGGCAGCGCTGTGTTTGACTTCAGCGGCACTGGGCCGGAGGTGTTTGGTAATCTCAACGCACC
  GCGGGCCGTAACCCTGTCCGCCCTCATCTACTGCCTGCGCTGTCTGGTGGGCCGCGACATCCCACTCAAC
  CAGGGCTGCCTGGCGCCAGTGCGCGTGGTCATTCCCCGAGGCTCCATCCTGGACCCGTCGCCCGAGGCGG
  CGGTGGTGGGCGGCAACGTGCTCACGTCGCAGCGCGTGGTGGATGTCATCCTGGGGGCCTTTGGGGCCTG
  CGCCGCCTCCCAGGGCTGCATGAACAACGTGACCCTGGGCAACGCCCACATGGGCTACTACGAGACGGTG
  GCGGGCGGCGCGGGCGCGGGTCCCAGCTGGCACGGGCGCAGCGGTGTGCACAGCCACATGACCAACACAC
  GCATCACCGACCCTGAGATCCTGGAGAGCCGGTACCCGGTCATCCTGCGCCGCTTCGAGCTGCGGCGGGG
  CTCGGGGGGCAGAGGCCGCTTCCGAGGCGGCGACGGCGTCACCCGCGAGCTGCTCTTTCGTGAGGAGGCG
  CTGCTGTCAGTGCTGACCGAGCGCCGCGCCTTCCGGCCATACGGGCTCCACGGGGGCGAGCCTGGCGCCC
  GCGGCCTAAACCTGCTGATCCGCAAAAACGGCCGGACGGTGAATCTGGGCGGCAAGACGTCGGTGACCGT
  GTACCCCGGGGATGTGTTCTGTCTCCACACGCCCGGCGGCGGTGGCTATGGGGACCCGGAGGACCCCGCC
  CCACCGCCGGGGTCGCCCCCGCAAGCACTGGCCTTTCCCGAGCACGGCAGCGTCTATGAGTATCGCCGGG
  CCCAGGAGGCCGTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG219080 representing NM_017570
  Red=Cloning site Green=Tags(s)
  
  MGSPEGRFHFAIDRGGTFTDVFAQCPGGHVRVLKLLSEDPANYADAPTEGIRRILEQEAGMLLPRDQPLD
  SSHIASIRMGTTVATNALLERKGERVALLVTRGFRDLLHIGTQARGDLFDLAVPMPEVLYEEVLEVDERV
  VLHRGEAGTGTPVKGRTGDLLEVQQPVDLGALRGKLEGLLSRGIRSLAVVLMHSYTWAQHEQQVGVLARE
  LGFTHVSLSSEAMPMVRIVPRGHTACADAYLTPAIQRYVQGFCRGFQGQLKDVQVLFMRSDGGLAPMDTF
  SGSSAVLSGPAGGVVGYSATTYQQEGGQPVIGFDMGGTSTDVSRYAGEFEHVFEASTAGVTLQAPQLDIN
  TVAAGGGSRLFFRSGLFVVGPESAGAHPGPACYRKGGPVTVTDANLVLGRLLPASFPCIFGPGENQPLSP
  EASRKALEAVATEVNSFLTNGPCPASPLSLEEVAMGFVRVANEAMCRPIRALTQARGHDPSAHVLACFGG
  AGGQHACAIARALGMDTVHIHRHSGLLSALGLALADVVHEAQEPCSLLYAPETFVQLDQRLSRLEEQCVD
  ALQAQGFPRSQISTESFLHLRYQGTDCALMVSAHQHPATARSPRAGDFGAAFVERYMREFGFVIPERPVV
  VDDVRVRGTGRSGLRLEDAPKAQTGPPRVDKMTQCYFEGGYQETPVYLLAELGYGHKLHGPCLIIDSNST
  ILVEPGCQAEVTKTGDICISVGAEVPGTVGPQLDPIQLSIFSHRFMSIAEQMGRILQRTAISTNIKERLD
  FSCALFGPDGGLVSNAPHIPVHLGAMQETVQFQIQHLGADLHPGDVLLSNHPSAGGSHLPDLTVITPVFW
  PGQTRPVFYVASRGHHADIGGITPGSMPPHSTMLQQEGAVFLSFKLVQGGVFQEEAVTEALRAPGKVPNC
  SGTRNLHDNLSDLRAQVAANQKGIQLVGELIGQYGLDVVQAYMGHIQANAELAVRDMLRAFGTSRQARGL
  PLEVSSEDHMDDGSPIRLRVQISLSQGSAVFDFSGTGPEVFGNLNAPRAVTLSALIYCLRCLVGRDIPLN
  QGCLAPVRVVIPRGSILDPSPEAAVVGGNVLTSQRVVDVILGAFGACAASQGCMNNVTLGNAHMGYYETV
  AGGAGAGPSWHGRSGVHSHMTNTRITDPEILESRYPVILRRFELRRGSGGRGRFRGGDGVTRELLFREEA
  LLSVLTERRAFRPYGLHGGEPGARGLNLLIRKNGRTVNLGGKTSVTVYPGDVFCLHTPGGGGYGDPEDPA
  PPPGSPPQALAFPEHGSVYEYRRAQEAV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017570
• ORF Size: 3864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017570.1, NP_060040.1
• RefSeq Size: 3944 bp
• RefSeq ORF: 3867 bp
• Locus ID: 26873
• UniProt ID: O14841
• Cytogenetics: 8q24.3
• Protein Pathways: Glutathione metabolism
• Gene Summary: The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD). [provided by RefSeq, Jun 2012]