DNA polymerase alpha (POLA1) (NM_016937) Human Untagged Clone

CAT#: SC304453

POLA1 (untagged)-Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLA1
• Synonyms: NSX; p180; POLA; VEODS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016937 edited
  GACCATGGCACCTGTGCACGGCGACGACTCTCTGTCAGATTCAGGGAGTTTTGTATCTTC
  TCGAGCCCGGCGAGAAAAAAAATCAAAGAAGGGGCGCCAAGAAGCCCTAGAAAGACTGAA
  AAAGGCTAAAGCTGGTGAGAAGTATAAATATGAAGTCGAGGACTTCACAGGTGTTTATGA
  AGAAGTTGATGAAGAACAGTATTCGAAGCTGGTTCAGGCACGCCAGGATGATGACTGGAT
  TGTGGATGATGATGGTATTGGCTATGTGGAAGATGGCCGAGAGATTTTTGATGATGACCT
  TGAAGATGATGCCCTTGATGCTGATGAGAAAGGAAAAGATGGTAAAGCACGCAATAAAGA
  CAAGAGGAATGTAAAGAAGCTCGCAGTGACAAAACCGAACAACATTAAGTCAATGTTCAT
  TGCTTGTGCTGGAAAGAAAACTGCAGATAAAGCTGTAGACTTGTCCAAGGATGGTCTGCT
  AGGTGACATTCTACAGGATCTTAACACTGAGACACCTCAAATAACTCCACCACCTGTAAT
  GATACTGAAGAAGAAAAGATCCATTGGAGCTTCACCGAATCCTTTCTCTGTGCACACCGC
  CACGGCAGTTCCTTCAGGAAAAATTGCTTCCCCTGTCTCCAGAAAGGAGCCTCCATTAAC
  TCCTGTTCCTCTTAAACGTGCTGAATTTGCTGGCGATGATGTACAGGTCGAGAGTACAGA
  AGAAGAGCAGGAGTCAGGGGCAATGGAGTTTGAAGATGGTGACTTTGATGAGCCCATGGA
  AGTTGAAGAGGTGGACCTGGAGCCTATGGCTGCCAAGGCTTGGGACAAAGAGAGTGAGCC
  AGCAGAGGAAGTGAAACAAGAGGCGGATTCTGGGAAAGGGACCGTGTCCTACTTAGGAAG
  TTTTCTCCCGGATGTCTCTTGTTGGGACATTGATCAAGAAGGTGATAGCAGTTTCTCAGT
  GCAAGAAGTTCAAGTGGATTCCAGTCACCTCCCATTGGTAAAAGGGGCAGATGAGGAACA
  AGTATTCCACTTTTATTGGTTGGATGCTTATGAGGATCAGTACAACCAACCAGGTGTGGT
  ATTTCTGTTTGGGAAAGTTTGGATTGAATCAGCCGAGACCCATGTGAGCTGTTGTGTCAT
  GGTGAAAAATATCGAGCGAACGCTTTACTTCCTTCCCCGTGAAATGAAAATTGATCTAAA
  TACGGGGAAAGAAACAGGAACTCCAATTTCAATGAAGGATGTTTATGAGGAATTTGATGA
  GAAAATAGCAACAAAATATAAAATTATGAAGTTCAAGTCTAAGCCAGTGGAAAAGAACTA
  TGCTTTTGAGATACCTGATGTTCCAGAAAAATCTGAGTACTTGGAAGTTAAATACTCGGC
  TGAAATGCCACAGCTTCCTCAAGATTTGAAAGGAGAAACTTTTTCTCATGTATTTGGGAC
  CAACACATCTAGCCTGGAACTGTTCTTGATGAACAGAAAGATCAAAGGACCTTGTTGGCT
  TGAAGTAAAAAGTCCACAGCTCTTGAATCAGCCAGTCAGTTGGTGTAAAGTTGAGGCAAT
  GGCTTTGAAACCAGACCTGGTGAATGTAATTAAGGATGTCAGTCCACCACCGCTTGTCGT
  GATGGCTTTCAGCATGAAGACAATGCAGAATGCAAAGAACCATCAAAATGAGATTATTGC
  TATGGCAGCTTTGGTCCATCACAGTTTTGCATTGGATAAAGCAGCCCCAAAGCCTCCCTT
  TCAGTCACACTTCTGTGTTGTGTCTAAACCAAAGGACTGTATTTTTCCATATGCTTTCAA
  AGAAGTCATTGAGAAAAAGAATGTGAAGGTTGAGGTTGCTGCAACAGAAAGAACACTGCT
  AGGTTTTTTCCTTGCAAAAGTTCACAAAATTGATCCTGATATCATTGTGGGTCATAATAT
  TTATGGGTTTGAACTGGAAGTACTACTGCAGAGAATTAATGTGTGCAAAGCTCCTCACTG
  GTCCAAGATAGGTCGACTGAAGCGATCCAACATGCCAAAGCTTGGGGGCCGGAGTGGATT
  TGGTGAAAGAAATGCTACCTGTGGTCGAATGATCTGTGATGTGGAAATTTCAGCAAAGGA
  ATTGATTCGTTGTAAAAGCTACCATCTGTCTGAACTTGTTCAGCAGATTCTAAAAACTGA
  AAGGGTTGTAATCCCAATGGAAAATATACAAAATATGTACAGTGAATCTTCTCAACTGTT
  ATACCTGTTGGAACACACCTGGAAAGATGCCAAGTTCATTTTGCAGATCATGTGTGAGCT
  AAATGTTCTTCCATTAGCATTGCAGATCACTAACATCGCTGGGAACATTATGTCCAGGAC
  GCTGATGGGTGGACGATCCGAGCGTAACGAGTTCTTGTTGCTTCATGCATTTTACGAAAA
  CAACTATATTGTGCCTGACAAGCAGATTTTCAGAAAGCCTCAGCAAAAACTGGGAGATGA
  AGATGAAGAAATTGATGGAGATACCAATAAATACAAGAAAGGACGTAAGAAAGCAGCTTA
  TGCTGGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGATAAGTTCATTTTGCTTCT
  GGACTTCAACAGTCTATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGT
  ACAAAGAGTTGCTTCAGAGGCACAGAAAGTTACAGAGGATGGAGAACAAGAACAGATCCC
  TGAGTTGCCAGATCCAAGCTTAGAAATGGGCATTTTGCCCAGAGAGATCCGGAAACTGGT
  AGAACGGAGAAAACAAGTCAAACAGCTAATGAAACAGCAAGACTTAAATCCAGACCTTAT
  TCTTCAGTATGACATTCGACAGAAGGCTTTGAAGCTCACAGCGAACAGTATGTATGGTTG
  CCTGGGATTTTCCTATAGCAGATTTTACGCCAAACCACTGGCTGCCTTGGTGACATACAA
  AGGAAGGGAGATTTTGATGCATACGAAAGAGATGGTACAAAAGATGAATCTTGAAGTTAT
  TTATGGAGATACAGATTCAATTATGATAAACACCAATAGCACCAATCTGGAAGAAGTATT
  TAAGTTGGGAAACAAGGTAAAAAGTGAAGTGAATAAGTTGTACAAACTGCTTGAAATAGA
  CATTGATGGGGTTTTCAAGTCTCTGCTACTGCTGAAAAAAAAGAAGTACGCTGCTCTGGT
  TGTTGAGCCAACGTCGGATGGGAATTATGTCACCAAACAGGAGCTCAAAGGATTAGATAT
  AGTTAGAAGAGATTGGTGTGATCTTGCTAAAGACACTGGAAACTTTGTGATTGGCCAGAT
  TCTTTCTGATCAAAGCCGGGACACTATAGTGGAAAACATTCAGAAGAGGCTGATAGAAAT
  TGGAGAAAATGTGCTAAATGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACAAGGCATT
  GACAAAGGATCCCCAGGATTACCCTGATAAAAAAAGCCTACCTCATGTACATGTTGCCCT
  CTGGATAAATTCTCAAGGAGGCAGAAAGGTGAAAGCTGGAGATACTGTGTCATATGTCAT
  CTGTCAGGATGGATCAAACCTCACTGCAAGTCAGAGGGCCTATGCGCCTGAGCAGCTGCA
  GAAACAGGATAATCTAACCATTGACACCCAGTACTACCTGGCCCAGCAGATCCACCCAGT
  CGTGGCTCGGATCTGTGAACCAATAGACGGAATTGATGCTGTCCTCATTGCAACGTGGTT
  GGGACTTGACCCCACCCAATTTAGAGTTCATCATTATCATAAAGATGAAGAGAATGATGC
  TCTACTTGGTGGCCCAGCACAGCTCACTGATGAAGAGAAATACAGGGACTGTGAAAGATT
  CAAATGTCCATGCCCTACATGTGGAACTGAGAATATTTATGATAATGTCTTTGATGGTTC
  GGGAACAGATATGGAGCCCAGCTTGTATCGTTGCAGTAACATCGATTGTAAGGCTTCACC
  TCTGACCTTTACAGTACAACTGAGCAACAAATTGATCATGGACATTAGACGTTTCATTAA
  AAAGTACTATGATGGCTGGTTGATATGTGAAGAGCCAACCTGTCGCAATCGAACTCGTCA
  CCTTCCCCTTCAATTCTCCCGAACTGGGCCTCTTTGCCCAGCCTGCATGAAAGCTACACT
  TCAACCAGAGTATTCTGACAAGTCCCTGTACACCCAGCTGTGCTTTTACCGGTACATTTT
  TGATGCGGAGTGTGCACTGGAGAAACTTACTACCGATCATGAGAAAGATAAATTGAAGAA
  GCAATTTTTTACCCCCAAAGTTCTGCAGGACTACAGAAAACTCAAGAACACAGCAGAGCA
  ATTCTTGTCCCGAAGTGGCTACTCCGAAGTGAATCTGAGCAAACTCTTCGCTGGTTGTGC
  CGTGAAATCCTAAGGGAATCCCAGGAGTAACCAAGGAGGGGGTAGTTGAAAAATCCCAGC
  TTCCTCTGTGCCTCCACTCTGGCCCTAAATGCTCCTCCAGCATCTGTTTCTCCCTTGGGA
  CTGTGTCTCATGTTTGTGTGAATGTAGACCAGGAAAGGGGGCTGCAAAAATGTTGAGTCT
  AATGTTCGTAAGCATCATAGAAATTCCTGTCTTCATATTAAGATGTACTGCTTTAAAACA
  CAACTCCAGAGCCCCTCCCCAAGCTCCCCTCCCCAAGCTCCTGAAGACCCGGTTTCTGAG
  GGAGGGAAATTGCTACTTGGATTGAGAGTAGCTGGAATGTAAGTGACCCCAGGCTTTGCC
  TCAGGGCCTTTAGCCTATGTCCCCCCCACATAAAGAGAGCTTCTCAGAGCCTGACTGAAG
  AGCTGACGTTTTGCTTTTTCATATGCCAATTAAACCCGGTCTAAATCCAAATGCTTCTCC
  AGCCATCCAGGAGTGGCTGTCCTTTTCAGTCTTGTCTTTTATATAGGTAGCTGAGGGGGA
  AGATTTAGAAGCCTTGCACTCACTAAATAGATTAAACAGAGCAGGCTTGTTTGTTGAATT
  GCTCCAAAGTCCAACAGACACACACTGAGCAGGTGTTTTACACTCACATTCCCTTTTTGC
  CCCTTAAATAGAAAGTGCAGGTAAAGGTTTATACAACAAGAAAGCACATTGAAAATAATT
  TGATACTCTAACAATCCATTAACATGTGTAGGGGTTACGGTGAGGATCACTGTGTTGTAT
  TCAGAAAAACGGGGAGAGGGATGCTTAATTGGCCCTGGCGCTTGCTATTTTTTTCTCATT
  TCTTCACAATAGGACCGTCTTTGGCAGCAGCAAAATGTATTTCAGTATGGCAGTCTTTCC
  TCTCTTACATTATTGGTAAGATTATACTAACAAAATGTTTCCCCTTGTACAATTATGCTG
  TGTTTTTAAAAAACATTGACCTGTGTGTTTTTATAAAAGAAAAAGTATGTTGTGCCTTCT
  TCTTAAGAATAAAGTTTTCTAAAGGGAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_016937
• Insert Size: 5500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: It is not a varient. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016937.1, NP_058633.1
• RefSeq Size: 5433 bp
• RefSeq ORF: 4389 bp
• Locus ID: 5422
• UniProt ID: P09884
• Cytogenetics: Xp22.11-p21.3
• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.