DNA polymerase alpha (POLA1) (NM_016937) Human Tagged ORF Clone

CAT#: RG214011

POLA1 (tGFP-tagged) - Human polymerase (DNA directed), alpha 1, catalytic subunit (POLA1)

Product Images

Circular map for RG214011

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: DNA polymerase alpha
• Synonyms: NSX; p180; POLA; VEODS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214011 representing NM_016937
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACCTGTGCACGGCGACGACTCTCTGTCAGATTCAGGGAGTTTTGTATCTTCTCGAGCCCGGCGAG
  AAAAAAAATCAAAGAAGGGGCGCCAAGAAGCCCTAGAAAGACTGAAAAAGGCTAAAGCTGGTGAGAAGTA
  TAAATATGAAGTCGAGGACTTCACAGGTGTTTATGAAGAAGTTGATGAAGAACAGTATTCGAAGCTGGTT
  CAGGCACGCCAGGATGATGACTGGATTGTGGATGATGATGGTATTGGCTATGTGGAAGATGGCCGAGAGA
  TTTTTGATGATGACCTTGAAGATGATGCCCTTGATGCTGATGAGAAAGGAAAAGATGGTAAAGCACGCAA
  TAAAGACAAGAGGAATGTAAAGAAGCTCGCAGTGACAAAACCGAACAACATTAAGTCAATGTTCATTGCT
  TGTGCTGGAAAGAAAACTGCAGATAAAGCTGTAGACTTGTCCAAGGATGGTCTGCTAGGTGACATTCTAC
  AGGATCTTAACACTGAGACACCTCAAATAACTCCACCACCTGTAATGATACTGAAGAAGAAAAGATCCAT
  TGGAGCTTCACCGAATCCTTTCTCTGTGCACACCGCCACGGCAGTTCCTTCAGGAAAAATTGCTTCCCCT
  GTCTCCAGAAAGGAGCCTCCATTAACTCCTGTTCCTCTTAAACGTGCTGAATTTGCTGGCGATGATGTAC
  AGGTCGAGAGTACAGAAGAAGAGCAGGAGTCAGGGGCAATGGAGTTTGAAGATGGTGACTTTGATGAGCC
  CATGGAAGTTGAAGAGGTGGACCTGGAGCCTATGGCTGCCAAGGCTTGGGACAAAGAGAGTGAGCCAGCA
  GAGGAAGTGAAACAAGAGGCGGATTCTGGGAAAGGGACCGTGTCCTACTTAGGAAGTTTTCTCCCGGATG
  TCTCTTGTTGGGACATTGATCAAGAAGGTGATAGCAGTTTCTCAGTGCAAGAAGTTCAAGTGGATTCCAG
  TCACCTCCCATTGGTAAAAGGGGCAGATGAGGAACAAGTATTCCACTTTTATTGGTTGGATGCTTATGAG
  GATCAGTACAACCAACCAGGTGTGGTATTTCTGTTTGGGAAAGTTTGGATTGAATCAGCCGAGACCCATG
  TGAGCTGTTGTGTCATGGTGAAAAATATCGAGCGAACGCTTTACTTCCTTCCCCGTGAAATGAAAATTGA
  TCTAAATACGGGGAAAGAAACAGGAACTCCAATTTCAATGAAGGATGTTTATGAGGAATTTGATGAGAAA
  ATAGCAACAAAATATAAAATTATGAAGTTCAAGTCTAAGCCAGTGGAAAAGAACTATGCTTTTGAGATAC
  CTGATGTTCCAGAAAAATCTGAGTACTTGGAAGTTAAATACTCGGCTGAAATGCCACAGCTTCCTCAAGA
  TTTGAAAGGAGAAACTTTTTCTCATGTATTTGGGACCAACACATCTAGCCTGGAACTGTTCTTGATGAAC
  AGAAAGATCAAAGGACCTTGTTGGCTTGAAGTAAAAAAGTCCACAGCTCTTAATCAGCCAGTCAGTTGGT
  GTAAAGTTGAGGCAATGGCTTTGAAACCAGACCTGGTGAATGTAATTAAGGATGTCAGTCCACCACCGCT
  TGTCGTGATGGCTTTCAGCATGAAGACAATGCAGAATGCAAAGAACCATCAAAATGAGATTATTGCTATG
  GCAGCTTTGGTCCATCACAGTTTTGCATTGGATAAAGCAGCCCCAAAGCCTCCCTTTCAGTCACACTTCT
  GTGTTGTGTCTAAACCAAAGGACTGTATTTTTCCATATGCTTTCAAAGAAGTCATTGAGAAAAAGAATGT
  GAAGGTTGAGGTTGCTGCAACAGAAAGAACACTGCTAGGTTTTTTCCTTGCAAAAGTTCACAAAATTGAT
  CCTGATATCATTGTGGGTCATAATATTTATGGGTTTGAACTGGAAGTACTACTGCAGAGAATTAATGTGT
  GCAAAGCTCCTCACTGGTCCAAGATAGGTCGACTGAAGCGATCCAACATGCCAAAGCTTGGGGGCCGGAG
  TGGATTTGGTGAAAGAAATGCTACCTGTGGTCGAATGATCTGTGATGTGGAAATTTCAGCAAAGGAATTG
  ATTCGTTGTAAAAGCTACCATCTGTCTGAACTTGTTCAGCAGATTCTAAAAACTGAAAGGGTTGTAATCC
  CAATGGAAAATATACAAAATATGTACAGTGAATCTTCTCAACTGTTATACCTGTTGGAACACACCTGGAA
  AGATGCCAAGTTCATTTTGCAGATCATGTGTGAGCTAAATGTTCTTCCATTAGCATTGCAGATCACTAAC
  ATCGCTGGGAACATTATGTCCAGGACGCTGATGGGTGGACGATCCGAGCGTAACGAGTTCTTGTTGCTTC
  ATGCATTTTACGAAAACAACTATATTGTGCCTGACAAGCAGATTTTCAGAAAGCCTCAGCAAAAACTGGG
  AGATGAAGATGAAGAAATTGATGGAGATACCAATAAATACAAGAAAGGACGTAAGAAAGGAGCTTATGCT
  GGAGGCTTGGTTTTGGACCCCAAAGTTGGTTTTTATGATAAGTTCATTTTGCTTCTGGACTTCAACAGTC
  TATATCCTTCCATCATTCAGGAATTTAACATTTGTTTTACAACAGTACAAAGAGTTGCTTCAGAGGCACA
  GAAAGTTACAGAGGATGGAGAACAAGAACAGATCCCTGAGTTGCCAGATCCAAGCTTAGAAATGGGCATT
  TTGCCCAGAGAGATCCGGAAACTGGTAGAACGGAGAAAACAAGTCAAACAGCTAATGAAACAGCAAGACT
  TAAATCCAGACCTTATTCTTCAGTATGACATTCGACAGAAGGCTTTGAAGCTCACAGCGAACAGTATGTA
  TGGTTGCCTGGGATTTTCCTATAGCAGATTTTACGCCAAACCACTGGCTGCCTTGGTGACATACAAAGGA
  AGGGAGATTTTGATGCATACGAAAGAGATGGTACAAAAGATGAATCTTGAAGTTATTTATGGAGATACAG
  ATTCAATTATGATAAACACCAATAGCACCAATCTGGAAGAAGTATTTAAGTTGGGAAACAAGGTAAAAAG
  TGAAGTGAATAAGTTGTACAAACTGCTTGAAATAGACATTGATGGGGTTTTCAAGTCTCTGCTACTGCTG
  AAAAAAAAGAAGTACGCTGCTCTGGTTGTTGAGCCAACGTCGGATGGGAATTATGTCACCAAACAGGAGC
  TCAAAGGATTAGATATAGTTAGAAGAGATTGGTGTGATCTTGCTAAAGACACTGGAAACTTTGTGATTGG
  CCAGATTCTTTCTGATCAAAGCCGGGACACTATAGTGGAAAACATTCAGAAGAGGCTGATAGAAATTGGA
  GAAAATGTGCTAAATGGCAGTGTCCCAGTGAGCCAGTTTGAAATTAACAAGGCATTGACAAAGGATCCCC
  AGGATTACCCTGATAAAAAAAGCCTACCTCATGTACATGTTGCCCTCTGGATAAATTCTCAAGGAGGCAG
  AAAGGTGAAAGCTGGAGATACTGTGTCATATGTCATCTGTCAGGATGGATCAAACCTCACTGCAAGTCAG
  AGGGCCTATGCGCCTGAGCAGCTGCAGAAACAGGATAATCTAACCATTGACACCCAGTACTACCTGGCCC
  AGCAGATCCACCCAGTCGTGGCTCGGATCTGTGAACCAATAGACGGAATTGATGCTGTCCTCATTGCAAC
  GTGGTTGGGACTTGACCCCACCCAATTTAGAGTTCATCATTATCATAAAGATGAAGAGAATGATGCTCTA
  CTTGGTGGCCCAGCACAGCTCACTGATGAAGAGAAATACAGGGACTGTGAAAGATTCAAATGTCCATGCC
  CTACATGTGGAACTGAGAATATTTATGATAATGTCTTTGATGGTTCGGGAACAGATATGGAGCCCAGCTT
  GTATCGTTGCAGTAACATCGATTGTAAGGCTTCACCTCTGACCTTTACAGTACAACTGAGCAACAAATTG
  ATCATGGACATTAGACGTTTCATTAAAAAGTACTATGATGGCTGGTTGATATGTGAAGAGCCAACCTGTC
  GCAATCGAACTCGTCACCTTCCCCTTCAATTCTCCCGAACTGGGCCTCTTTGCCCAGCCTGCATGAAAGC
  TACACTTCAACCAGAGTATTCTGACAAGTCCCTGTACACCCAGCTGTGCTTTTACCGGTACATTTTTGAT
  GCGGAGTGTGCACTGGAGAAACTTACTACCGATCATGAGAAAGATAAATTGAAGAAGCAATTTTTTACCC
  CCAAAGTTCTGCAGGACTACAGAAAACTCAAGAACACAGCAGAGCAATTCTTGTCCCGAAGTGGCTACTC
  CGAAGTGAATCTGAGCAAACTCTTCGCTGGTTGTGCCGTGAAATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214011 representing NM_016937
  Red=Cloning site Green=Tags(s)
  
  MAPVHGDDSLSDSGSFVSSRARREKKSKKGRQEALERLKKAKAGEKYKYEVEDFTGVYEEVDEEQYSKLV
  QARQDDDWIVDDDGIGYVEDGREIFDDDLEDDALDADEKGKDGKARNKDKRNVKKLAVTKPNNIKSMFIA
  CAGKKTADKAVDLSKDGLLGDILQDLNTETPQITPPPVMILKKKRSIGASPNPFSVHTATAVPSGKIASP
  VSRKEPPLTPVPLKRAEFAGDDVQVESTEEEQESGAMEFEDGDFDEPMEVEEVDLEPMAAKAWDKESEPA
  EEVKQEADSGKGTVSYLGSFLPDVSCWDIDQEGDSSFSVQEVQVDSSHLPLVKGADEEQVFHFYWLDAYE
  DQYNQPGVVFLFGKVWIESAETHVSCCVMVKNIERTLYFLPREMKIDLNTGKETGTPISMKDVYEEFDEK
  IATKYKIMKFKSKPVEKNYAFEIPDVPEKSEYLEVKYSAEMPQLPQDLKGETFSHVFGTNTSSLELFLMN
  RKIKGPCWLEVKKSTALNQPVSWCKVEAMALKPDLVNVIKDVSPPPLVVMAFSMKTMQNAKNHQNEIIAM
  AALVHHSFALDKAAPKPPFQSHFCVVSKPKDCIFPYAFKEVIEKKNVKVEVAATERTLLGFFLAKVHKID
  PDIIVGHNIYGFELEVLLQRINVCKAPHWSKIGRLKRSNMPKLGGRSGFGERNATCGRMICDVEISAKEL
  IRCKSYHLSELVQQILKTERVVIPMENIQNMYSESSQLLYLLEHTWKDAKFILQIMCELNVLPLALQITN
  IAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRKPQQKLGDEDEEIDGDTNKYKKGRKKGAYA
  GGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICFTTVQRVASEAQKVTEDGEQEQIPELPDPSLEMGI
  LPREIRKLVERRKQVKQLMKQQDLNPDLILQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKG
  REILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEVNKLYKLLEIDIDGVFKSLLLL
  KKKKYAALVVEPTSDGNYVTKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIG
  ENVLNGSVPVSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVICQDGSNLTASQ
  RAYAPEQLQKQDNLTIDTQYYLAQQIHPVVARICEPIDGIDAVLIATWLGLDPTQFRVHHYHKDEENDAL
  LGGPAQLTDEEKYRDCERFKCPCPTCGTENIYDNVFDGSGTDMEPSLYRCSNIDCKASPLTFTVQLSNKL
  IMDIRRFIKKYYDGWLICEEPTCRNRTRHLPLQFSRTGPLCPACMKATLQPEYSDKSLYTQLCFYRYIFD
  AECALEKLTTDHEKDKLKKQFFTPKVLQDYRKLKNTAEQFLSRSGYSEVNLSKLFAGCAVKS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016937
• ORF Size: 4386 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016937.1, NP_058633.1
• RefSeq Size: 5433 bp
• RefSeq ORF: 4389 bp
• Locus ID: 5422
• UniProt ID: P09884
• Cytogenetics: Xp22.11-p21.3
• Protein Families: Druggable Genome
• Protein Pathways: DNA replication, Metabolic pathways, Purine metabolism, Pyrimidine metabolism
• Gene Summary: This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]