CEP250 (NM_007186) Human Untagged Clone

CAT#: SC303890

CEP250 (untagged)-Human centrosomal protein 250kDa (CEP250)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CEP250
• Synonyms: C-NAP1; CEP2; CNAP1; CRDHL2
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007186, the custom clone sequence may differ by one or more nucleotides
  ATGGAGACAAGAAGCCCTGGGTTGAACAACATGAAGCCCCAGTCACTGCAGCTGGTACTG
  GAAGAGCAGGTGCTGGCACTACAGCAGCAGATGGCAGAGAATCAGGCAGCCTCCTGGCGG
  AAGCTGAAGAACTCCCAGGAGGCCCAGCAGAGACAAGCAACCCTTGTGAGGAAGCTGCAG
  GCCAAGGTGCTGCAGTACCGAAGCTGGTGCCAAGAGCTGGAGAAGCGGCTAGAAGCCACT
  GGAGGACCAATCCCCCAGAGGTGGGAAAATGTGGAGGAGCCAAACCTGGATGAGCTGCTG
  GTCCGATTGGAGGAGGAGCAACAGAGGTGTGAGAGTCTAGCAGAGGTGAACACCCAGCTT
  CGACTGCACATGGAAAAAGCTGACGTGGTGAATAAAGCCCTTAGGGAAGATGTGGAAAAA
  CTGACAGTGGACTGGAGCCGGGCCCGGGATGAGCTAATGAGGAAGGAGAGCCAGTGGCAG
  ATGGAGCAGGAGTTCTTCAAGGGCTACCTGAAAGGGGAGCACGGTCGCCTTCTCAGTCTA
  TGGCGGGAGGTTGTGACATTCCGACGCCACTTCCTGGAAATGAAGTCAGCTACTGACAGA
  GATCTGATGGAGCTAAAAGCTGAGCATGTGAGGCTTTCAGGGTCTCTGTTGACCTGTTGT
  CTGCGCTTGACTGTGGGAGCACAGTCTCGGGAACCCAACGGATCTGGAAGAATGGATGGG
  CGGGAGCCGGCCCAGCTGCTGCTGCTACTAGCCAAGACCCAGGAGCTGGAGAAGGAAGCC
  CATGAAAGGAGCCAGGAGTTAATACAGCTGAAGAGTCAAGGGGATCTGGAGAAGGCTGAA
  CTTCAGGACCGGGTGACCGAGCTCTCTGCTCTGTTGACCCAGTCTCAGAAGCAAAATGAA
  GATTATGAAAAGATGATAAAGGCTCTGAGAGAGACAGTGGAGATCCTGGAGACAAATCAC
  ACAGAATTAATGGAACATGAAGCATCTCTTAGTAGGAATGCGCAAGAGGAGAAGTTGTCT
  TTACAGCAGGTGATCAAGGATATAACCCAGGTCATGGTGGAAGAAGGGGACAATATAGCC
  CAAGGCTCTGGTCATGAGAACTCTTTGGAATTGGACTCTAGTATCTTCTCCCAGTTTGAT
  TACCAGGATGCAGACAAGGCTCTTACTCTGGTGCGTTCAGTGCTGACTCGGAGACGCCAG
  GCTGTGCAGGACCTAAGGCAGCAGCTTGCAGGCTGTCAAGAGGCTGTGAACTTGTTGCAA
  CAGCAGCATGATCAGTGGGAGGAAGAGGGCAAAGCCTTGAGACAGCGGCTGCAGAAGCTC
  ACTGGGGAGCGGGACACTCTGGCAGGGCAGACTGTGGACCTCCAGGGAGAGGTGGACTCT
  CTCAGCAAGGAGCGAGAGCTGCTGCAGAAGGCCAGGGAAGAGCTGCGGCAGCAGCTGGAG
  GTGCTAGAGCAGGAGGCATGGCGCCTGCGAAGGGTAAATGTGGAGCTTCAGCTGCAGGGG
  GACTCTGCCCAGGGCCAGAAGGAGGAACAGCAGGAGGAGCTGCACCTGGCTGTCCGGGAG
  AGGGAGCGTCTTCAGGAGATGCTGATGGGCCTGGAAGCCAAACAGTCAGAATCACTCAGT
  GAACTGATCACTCTTCGGGAAGCCCTGGAGTCAAGTCACCTGGAAGGGGAGTTACTGAGG
  CAAGAGCAAACGGAAGTGACCGCAGCGCTGGCTAGGGCAGAGCAGTCAATTGCAGAGCTG
  TCGAGTTCTGAAAACACCCTGAAGACAGAAGTAGCTGATCTTCGGGCTGCAGCTGTCAAG
  CTCAGTGCCTTAAATGAGGCTTTGGCGTTAGATAAAGTTGGGCTGAACCAGCAGCTTCTC
  CAGTTAGAGGAGGAGAACCAGTCTGTGTGCAGCAGAATGGAGGCCGCAGAGCAGGCGAGA
  AATGCTTTGCAGGTCGACCTGGCGGAGGCAGAGAAGAGGAGGGAAGCCCTGTGGGAAAAG
  AACACTCACCTGGAGGCTCAGCTGCAGAAAGCTGAGGAGGCTGGGGCTGAGCTGCAGGCA
  GATCTCAGGGACATCCAAGAAGAGAAGGAAGAAATTCAAAAGAAACTAAGTGAGTCACGT
  CACCAGCAGGAGGCAGCCACGACTCAGCTGGAGCAGCTACATCAGGAGGCAAAGCGACAG
  GAAGAAGTGCTTGCCAGGGCAGTCCAGGAGAAGGAGGCCCTAGTACGAGAGAAAGCGGCT
  CTAGAGGTGCGGCTGCAGGCCGTGGAGCGTGACCGGCAGGACCTCGCTGAACAACTACAG
  GGGCTCAGCTCAGCCAAGGAGCTACTGGAGAGCAGTCTGTTTGAAGCCCAACAACAAAAT
  TCTGTGATAGAGGTCACCAAGGGGCAGCTGGAGGTCCAGATTCAAACTGTCACTCAAGCC
  AAGGAAGTAATCCAAGGGGAAGTGAGGTGCCTGAAGCTGGAACTGGACACTGAACGGAGT
  CAGGCAGAGCAGGAGCGGGATGCTGCAGCCAGACAGCTGGCCCAGGCTGAGCAAGAAGGG
  AAGACTGCCTTGGAGCAGCAGAAGGCAGCCCATGAGAAAGAGGTGAACCAGCTCCGGGAG
  AAATGGGAGAAGGAGCGCTCCTGGCACCAGCAGGAGCTGGCAAAGGCTCTGGAGAGCTTA
  GAAAGGGAAAAAATGGAGCTGGAAATGAGGCTAAAGGAGCAGCAGACAGAAATGGAGGCC
  ATCCAGGCCCAGAGGGAAGAAGAACGGACCCAGGCAGAGAGTGCCCTATGCCAGATGCAG
  CTGGAAACAGAGAAGGAGAGAGTATCCCTCCTGGAGACACTGCTGCAGACGCAGAAGGAG
  CTAGCAGATGCCAGCCAACAACTGGAACGACTGAGGCAGGACATGAAAGTCCAGAAATTA
  AAGGAGCAGGAGACCACTGGGATACTACAGACCCAGCTCCAGGAGGCTCAACGGGAGCTG
  AAGGAGGCAGCCCGGCAGCACAGAGATGACCTTGCTGCCCTCCAAGAAGAGAGCAGCTCC
  CTGCTGCAGGATAAGATGGACCTGCAGAAGCAGGTGGAGGACTTGAAGTCTCAGCTGGTG
  GCCCAGGATGACTCCCAGAGGCTGGTGGAGCAGGAGGTTCAGGAGAAGCTGAGAGAGACC
  CAGGAGTATAACCGAATTCAGAAGGAGCTGGAGAGAGAGAAAGCCAGCCTGACTCTGTCA
  CTGATGGAAAAGGAACAGAGACTCCTTGTTTTACAAGAAGCTGACTCTATTCGACAACAA
  GAGCTGAGTGCCCTGCGCCAGGACATGCAGGAGGCCCAGGGAGAACAGAAAGAGCTCAGT
  GCTCAGATGGAATTACTAAGGCAAGAGGTGAAGGAAAAGGAGGCTGACTTTCTGGCCCAG
  GAAGCACAGCTGCTGGAGGAGCTGGAGGCGTCTCATATCACGGAGCAGCAGCTGCGAGCC
  TCCTTGTGGGCCCAGGAAGCCAAGGCAGCCCAACTACAGCTGCGACTGCGCAGCACAGAG
  AGCCAGCTAGAAGCGCTGGCCGCAGAGCAGCAGCCCGGGAACCAGGCCCAGGCCCAGGCC
  CAGCTGGCCAGCCTCTACTCTGCCCTGCAGCAGGCCCTGGGGTCTGTTTGTGAGAGCAGG
  CCTGAGCTGAGTGGTGGGGGAGACTCTGCTCCTTCCGTCTGGGGCCTTGAGCCAGACCAG
  AATGGAGCTAGGAGCCTCTTTAAGAGAGGGCCCCTGCTGACTGCTCTCTCCGCTGAGGCA
  GTAGCATCTGCCCTCCACAAGCTTCATCAAGACCTGTGGAAGACTCAACAGACCCGGGAT
  GTTCTGAGGGATCAGGTCCAGAAACTGGAAGAGCGTCTAACTGATACTGAGGCTGAGAAG
  AGCCAGGTCCACACAGAGTTGCAGGATCTGCAGAGACAGCTCTCCCAGAATCAGGAAGAG
  AAATCCAAGTGGGAAGGAAAGCAGAACTCCCTAGAATCTGAGCTGATGGAACTACATGAA
  ACTATGGCATCCTTACAGAGTCGCCTGCGGAGAGCAGAGCTACAGCGAATGGAAGCCCAG
  GGTGAGCGAGAGTTACTTCAGGCAGCCAAGGAGAACCTGACAGCCCAGGTGGAACACCTG
  CAAGCAGCTGTCGTAGAAGCCAGGGCTCAGGCAAGTGCTGCTGGCATCCTGGAAGAAGAC
  CTGAGAACGGCTCGCTCAGCACTGAAGCTGAAAAATGAGGAAGTAGAGAGTGAGCGTGAG
  AGAGCCCAGGCTCTGCAAGAGCAGGGCGAACTGAAGGTGGCCCAAGGGAAGGCTCTGCAA
  GAGAATTTGGCCCTCCTGACCCAGACCCTAGCTGAAAGAGAAGAGGAGGTGGAGACTCTG
  CGGGGACAAATCCAGGAACTGGAGAAGCAACGGGAAATGCAGAAGGCTGCTTTGGAATTG
  CTGTCTCTGGACCTGAAGAAGAGGAACCAAGAGGTAGATCTGCAGCAAGAACAGATTCAG
  GAGCTAGAGAAGTGTAGGTCTGTTTTAGAGCATCTGCCCATGGCCGTCCAGGAGCGAGAG
  CAGAAGCTGACTGTGCAGAGGGAGCAGATCAGAGAGCTCGAGAAGGATCGGGAGACTCAG
  AGGAACGTCTTGGAGCATCAGCTTCTAGAACTTGAGAAGAAAGACCAAATGATTGAGTCC
  CAGAGAGGACAGGTTCAGGACCTGAAAAAGCAGTTGGTTACTCTGGAATGCCTGGCCCTG
  GAACTGGAGGAAAACCATCACAAGATGGAGTGCCAGCAAAAACTGATCAAGGAGCTGGAG
  GGCCAGAGGGAAACCCAGAGAGTGGCTTTGACCCACCTTACGCTGGACCTAGAAGAAAGG
  AGCCAGGAGCTGCAGGCACAAAGCAGCCAGATCCATGACCTGGAGAGCCACAGCACCGTT
  CTGGCAAGAGAGCTGCAGGAGAGGGACCAGGAGGTGAAGTCTCAGCGAGAACAGATCGAG
  GAGCTGCAGAGGCAGAAAGAGCATCTGACTCAGGATCTCGAGAGGAGAGACCAGGAGCTG
  ATGCTGCAGAAGGAGAGGATTCAGGTTCTCGAGGATCAGAGGACCCGGCAGACCAAGATC
  CTGGAGGAGGACCTGGAACAGATCAAGCTGTCCTTGAGAGAGCGAGGCCGGGAGCTGACC
  ACTCAGAGGCAGCTGATGCAGGAACGGGCAGAGGAAGGGAAGGGCCCAAGTAAAGCACAG
  CGCGGGAGCCTAGAGCACATGAAGCTGATCCTGCGTGATAAGGAGAAGGAGGTGGAATGT
  CAGCAGGAGCATATCCATGAACTCCAGGAGCTCAAAGACCAGCTGGAGCAGCAGCTCCAG
  GGCCTGCACAGGAAGGTAGGTGAGACCAGCCTCCTCCTGTCCCAGCGAGAGCAGGAAATA
  GTGGTCCTGCAGCAGCAACTGCAGGAAGCCAGGGAACAAGGGGAGCTGAAGGAGCAGTCA
  CTTCAGAGTCAACTGGATGAGGCCCAGAGAGCCCTAGCCCAGAGGGACCAGGAACTGGAG
  GCTCTGCAGCAAGAACAGCAGCAGGCCCAGGGACAGGAGGAGAGGGTGAAGGAAAAGGCA
  GACGCCCTCCAGGGAGCTCTGGAGCAAGCCCATATGACACTGAAGGAGCGTCATGGAGAG
  CTTCAGGACCACAAGGAACAGGCACGAAGGCTGGAGGAAGAGCTGGCAGTGGAGGGACGG
  CGGGTCCAGGCCCTGGAGGAGGTGCTGGGAGACCTAAGGGCTGAGTCTCGGGAACAGGAG
  AAAGCTCTGTTGGCCCTCCAGCAGCAGTGTGCTGAGCAGGCACAGGAGCATGAGGTGGAG
  ACCAGGGCCCTGCAGGACAGCTGGCTGCAGGCCCAGGCAGTGCTCAAGGAACGGGACCAG
  GAGCTGGAAGCTCTGCGGGCAGAAAGTCAGTCCTCCCGGCATCAGGAGGAGGCTGCCCGG
  GCCCGGGCTGAGGCTCTGCAGGAGGCCCTTGGCAAGGCTCATGCTGCCCTGCAGGGGAAA
  GAGCAGCATCTCCTCGAGCAGGCAGAATTGAGCCGCAGTCTGGAGGCCAGCACTGCAACC
  CTGCAAGCCTCCCTGGATGCCTGCCAGGCACACAGTCGGCAGCTGGAGGAGGCTCTGAGG
  ATACAAGAAGGTGAGATCCAGGACCAGGATCTCCGATACCAGGAGGATGTGCAGCAGCTG
  CAGCAGGCACTTGCCCAGAGGGATGAAGAGCTGAGACATCAGCAGGAACGGGAGCAGCTG
  CTGGAGAAGTCTCTGGCCCAGAGGGTCCAAGAGAATATGATCCAAGAGAAGCAGAATCTG
  GGGCAAGAGAGAGAAGAGGAGGAGATAAGGGGCCTTCATCAGAGTGTAAGGGAGCTACAG
  CTGACTCTAGCCCAAAAGGAACAGGAGATTCTGGAGCTGAGGGAGACCCAGCAAAGGAAC
  AACCTGGAAGCCTTACCCCACAGCCACAAAACCTCCCCAATGGAGGAACAATCTCTAAAA
  CTTGATTCTTTAGAGCCCAGGCTGCAGCGGGAGCTGGAGCGGCTACAGGCAGCCCTGAGA
  CAGACAGAAGCCAGGGAGATTGAGTGGAGGGAGAAGGCCCAGGACTTGGCACTCTCCCTA
  GCGCAGACCAAGGCCAGTGTCAGCAGTCTGCAGGAGGTAGCCATGTTCCTACAAGCCTCT
  GTCCTGGAGCGGGACTCAGAACAGCAAAGGCTGCAGGATGAACTGGAGCTCACCAGACGG
  GCTCTGGAGAAGGAGCGGCTACACAGCCCAGGTGCAACCAGCACAGCAGAACTGGGGTCC
  AGAGGGGAGCAGGGTGTGCAGCTGGGAGAGGTCTCAGGAGTGGAGGCTGAGCCTAGTCCT
  GATGGAATGGAGAAGCAGTCATGGAGACAAAGGCTTGAACACCTGCAGCAAGCAGTGGCC
  CGGCTGGAGATTGACAGGAGCAGGCTGCAGCGCCACAATGTCCAGCTGCGGAGTACCTTG
  GAGCAGGTGGAGCGAGAACGGAGGAAGCTGAAGAGGGAGGCCATGCGTGCGGCCCAGGCA
  GGGTCCCTAGAGATCAGCAAGGCCACGGCTTCTTCACCCACACAGCAGGATGGGAGAGGA
  CAGAAGAACTCAGATGCCAAGTGTGTGGCTGAACTGCAGAAAGAGGTGGTCCTGCTGCAA
  GCTCAGCTGACTTTGGAGCGGAAGCAGAAGCAGGACTACATCACCCGCTCAGCACAGACC
  AGCCGTGAGCTAGCAGGCCTGCACCACAGCCTCTCACACTCACTTCTTGCCGTGGCCCAG
  GCCCCTGAGGCCACTGTCCTGGAGGCAGAGACCCGCAGGCTGGATGAGTCCCTGACTCAA
  AGTCTGACATCCCCAGGGCCAGTCCTGCTACACCCCAGCCCCAGCACTACCCAAGCCGCC
  TCCAGGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_007186
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007186.2, NP_009117.2
• RefSeq Size: 8365 bp
• RefSeq ORF: 7329 bp
• Locus ID: 11190
• UniProt ID: Q9BV73
• Cytogenetics: 20q11.22
• Gene Summary: This gene encodes a core centrosomal protein required for centriole-centriole cohesion during interphase of the cell cycle. The encoded protein dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
  Transcript Variant: This variant (1) encodes the longer isoform (1).