Meprin beta (MEP1B) (NM_005925) Human Untagged Clone

CAT#: SC303704

MEP1B (untagged)-Human meprin A, beta (MEP1B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Meprin beta
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005925 edited
  GGTACCCAACTGGAAGCTACAACATGGATTTATGGAATCTGTCTTGGTTTCTGTTCTTGG
  ATGCTCTTCTCGTGATTTCTGGCTTGGCAACTCCAGAAAACTTTGATGTAGATGGCGGAA
  TGGACCAGGACATATTTGATATCAATGAAGGTTTGGGACTGGATCTTTTTGAAGGTGACA
  TCAGACTTGATAGGGCACAAATTAGAAATTCCATCATTGGAGAAAAGTATAGATGGCCTC
  ATACCATTCCATATGTTCTAGAAGATAGCTTGGAAATGAATGCTAAGGGAGTTATCCTCA
  ATGCATTTGAACGTTATCGCCTTAAAACATGTATTGACTTTAAGCCTTGGGCTGGAGAAA
  CAAACTATATATCAGTGTTCAAGGGCAGTGGCTGCTGGTCTTCAGTAGGAAATAGGCGGG
  TTGGGAAGCAAGAACTTTCCATCGGGGCAAACTGTGACCGAATAGCAACAGTTCAACACG
  AGTTCCTCCACGCTCTGGGATTCTGGCATGAGCAGTCGCGTTCTGACCGGGATGACTATG
  TCAGGATAATGTGGGACAGAATTCTGTCAGGCAGAGAGCACAATTTTAACACCTATAGTG
  ACGATATATCAGATTCCCTGAATGTTCCCTATGATTACACTTCAGTAATGCACTACAGTA
  AAACTGCATTCCAAAATGGAACAGAGCCGACAATTGTCACAAGAATCTCAGACTTTGAGG
  ATGTGATCGGCCAACGAATGGATTTCAGTGACTCTGATCTCCTAAAGTTGAATCAACTGT
  ATAACTGCTCCTCTTCCTTGAGTTTTATGGACTCGTGCAGTTTTGAACTGGAAAATGTGT
  GTGGCATGATCCAAAGTTCAGGAGATAATGCTGACTGGCAACGGGTTTCACAGGTTCCCA
  GGGGGCCAGAGAGTGATCACTCCAACATGGGCCAGTGCCAAGGTTCTGGTTTCTTCATGC
  ATTTCGATAGCAGCTCTGTAAATGTGGGGGCCACAGCAGTGCTGGAAAGTAGAACGCTGT
  ACCCTAAAAGAGGATTTCAGTGCCTGCAATTTTACTTATATAACAGTGGCAGTGAAAGTG
  ATCAACTGAACATCTATATCAGGGAGTATTCTGCAGACAATGTGGATGGCAATTTAACCC
  TTGTGGAAGAAATAAAAGAAATACCCACTGGGAGCTGGCAACTTTATCATGTAACATTGA
  AAGTGACCAAGAAGTTTAGAGTGGTGTTTGAAGGACGCAAAGGCTCTGGTGCATCACTGG
  GTGGTCTGTCTATTGATGACATCAATCTTTCGGAAACACGGTGCCCTCATCATATCTGGC
  ATATAAGGAATTTCACACAGTTCATTGGCAGCCCAAATGGAACTCTGTATAGCCCTCCAT
  TTTACTCTTCTAAAGGTTATGCCTTTCAGATTTACTTAAATCTAGCCCATGTGACTAATG
  CAGGGATATATTTCCACTTGATCTCTGGAGCCAATGATGATCAATTACAGTGGCCATGTC
  CTTGGCAACAAGCCACAATGACACTTTTGGATCAAAATCCTGACATTCGACAGCGTATGT
  CCAATCAGCGGAGTATAACTACAGACCCATTTATGACCACCGATAATGGAAACTATTTCT
  GGGACAGGCCTTCCAAAGTGGGAACAGTGGCTTTGTTCCCTAATGGAACTCAGTTTAGAA
  GAGGTGGGGGCTATGGAACCAGTGCCTTTATAACCCACGAAAGGCTGAAAAGCAGAGATT
  TTATAAAAGGAGATGATGTTTATATCCTACTGACAGTGGAAGACATATCTCACCTCAACT
  CTACACAAATCCAGCTAACACCAGCCCCTAGTGTTCAAGACCTCTGCTCAAAAACCACCT
  GTAAAAATGACGGTGTCTGCACTGTTCGAGATGGCAAAGCTGAGTGCAGGTGCCAGTCAG
  GGGAAGACTGGTGGTACATGGGAGAAAGGTGTGAAAAGAGAGGCTCCACCCGAGACACCA
  TAGTCATTGCTGTTTCATCTACTGTTGCTGTGTTTGCCTTGATGCTGATCATCACCCTTG
  TCAGTGTCTATTGCACCAGGAAGAAATATCGTGAAAGGATGAGCTCAAATCGACCAAATT
  TGACTCCGCAAAATCAGCATGCTTTTTGAAGATTAACTCGACAATATGGCCAGCTAATGA
  AATTAAAAAGGATTCTTCATCATGGATTTCGCCTAAGTGAAAGCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_005925
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005925.1, NP_005916.1
• RefSeq Size: 2326 bp
• RefSeq ORF: 2103 bp
• Locus ID: 4225
• UniProt ID: Q16820
• Cytogenetics: 18q12.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).