Meprin beta (MEP1B) (NM_005925) Human Tagged ORF Clone

CAT#: RC216063

MEP1B (Myc-DDK-tagged)-Human meprin A, beta (MEP1B)

Product Images

Circular map for RC216063

• 

  Western blot validation of overexpression lysate (Cat# LY416979) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC216063 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Meprin beta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216063 representing NM_005925
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTTATGGAATCTGTCTTGGTTTCTGTTCTTGGATGCTCTTCTCGTGATTTCTGGCTTGGCAACTC
  CAGAAAACTTTGATGTAGATGGCGGAATGGACCAGGACATATTTGATATCAATGAAGGTTTGGGACTGGA
  TCTTTTTGAGGGTGACATCAGACTTGATAGGGCACAAATTAGAAATTCCATCATTGGAGAAAAGTATAGA
  TGGCCTCATACCATTCCATATGTTCTAGAAGATAGCTTGGAAATGAATGCTAAGGGAGTTATCCTCAATG
  CATTTGAACGTTATCGCCTTAAAACATGTATTGACTTTAAGCCTTGGGCTGGAGAAACAAACTATATATC
  AGTGTTCAAGGGCAGTGGCTGCTGGTCTTCAGTAGGAAATAGGCGGGTTGGGAAGCAAGAACTTTCCATC
  GGGGCAAACTGTGACCGAATAGCAACAGTTCAACACGAGTTCCTCCACGCTCTGGGATTCTGGCATGAGC
  AGTCGCGTTCTGACCGGGATGACTATGTCAGGATAATGTGGGACAGAATTCTGTCAGGCAGAGAGCACAA
  TTTTAACACCTATAGTGACGATATATCAGATTCCCTGAATGTTCCCTATGATTACACTTCAGTAATGCAC
  TACAGTAAAACTGCATTCCAAAATGGAACAGAGCCGACAATTGTCACAAGAATCTCAGACTTTGAGGATG
  TGATCGGCCAACGAATGGATTTCAGTGACTCTGATCTCCTAAAGTTGAATCAACTGTATAACTGCTCCTC
  TTCCTTGAGTTTTATGGACTCGTGCAGTTTTGAACTGGAAAATGTGTGTGGCATGATCCAAAGTTCAGGA
  GATAATGCTGACTGGCAACGGGTTTCACAGGTTCCCAGGGGGCCAGAGAGTGATCACTCCAACATGGGCC
  AGTGCCAAGGTTCTGGTTTCTTCATGCATTTCGATAGCAGCTCTGTAAATGTGGGGGCCACAGCAGTGCT
  GGAAAGTAGAACGCTGTACCCTAAAAGAGGATTTCAGTGCCTGCAATTTTACTTATATAACAGTGGCAGT
  GAAAGTGATCAACTGAACATCTATATCAGGGAGTATTCTGCAGACAATGTGGATGGCAATTTAACCCTTG
  TGGAAGAAATAAAAGAAATACCCACTGGGAGCTGGCAACTTTATCATGTAACATTGAAAGTGACCAAGAA
  GTTTAGAGTGGTGTTTGAAGGACGCAAAGGCTCTGGTGCATCACTGGGTGGTCTGTCTATTGATGACATC
  AATCTTTCGGAAACACGGTGCCCTCATCATATCTGGCATATAAGGAATTTCACACAGTTCATTGGCAGCC
  CAAATGGAACTCTGTATAGCCCTCCATTTTACTCTTCTAAAGGTTATGCCTTTCAGATTTACTTAAATCT
  AGCCCATGTGACTAATGCAGGGATATATTTCCACTTGATCTCTGGAGCCAATGATGATCAATTACAGTGG
  CCATGTCCTTGGCAACAAGCCACAATGACACTTTTGGATCAAAATCCTGACATTCGACAGCGTATGTCCA
  ATCAGCGGAGTATAACTACAGACCCATTTATGACCACCGATAATGGAAACTATTTCTGGGACAGGCCTTC
  TAAAGTGGGAACAGTGGCTTTGTTCTCTAATGGAACTCAGTTTAGAAGAGGTGGGGGCTATGGAACCAGT
  GCCTTTATAACCCACGAAAGGCTGAAAAGCAGAGATTTTATAAAAGGAGATGATGTTTATATCCTACTGA
  CAGTGGAAGACATATCTCACCTCAACTCTACACAAATCCAGCTAACACCAGCCCCTAGTGTTCAAGACCT
  CTGCTCAAAAACCACCTGTAAAAATGACGGTGTCTGCACTGTTCGAGATGGCAAAGCTGAGTGCAGGTGC
  CAGTCAGGGGAAGACTGGTGGTACATGGGAGAAAGGTGTGAAAAGAGAGGCTCCACCCGAGACACCATAG
  TCATTGCTGTTTCATCTACTGTTGCTGTGTTTGCCTTGATGCTGATCATCACCCTTGTCAGTGTCTATTG
  CACCAGGAAGAAATATCGTGAAAGGATGAGCTCAAATCGACCAAATTTGACTCCGCAAAATCAGCATGCT
  TTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216063 representing NM_005925
  Red=Cloning site Green=Tags(s)
  
  MDLWNLSWFLFLDALLVISGLATPENFDVDGGMDQDIFDINEGLGLDLFEGDIRLDRAQIRNSIIGEKYR
  WPHTIPYVLEDSLEMNAKGVILNAFERYRLKTCIDFKPWAGETNYISVFKGSGCWSSVGNRRVGKQELSI
  GANCDRIATVQHEFLHALGFWHEQSRSDRDDYVRIMWDRILSGREHNFNTYSDDISDSLNVPYDYTSVMH
  YSKTAFQNGTEPTIVTRISDFEDVIGQRMDFSDSDLLKLNQLYNCSSSLSFMDSCSFELENVCGMIQSSG
  DNADWQRVSQVPRGPESDHSNMGQCQGSGFFMHFDSSSVNVGATAVLESRTLYPKRGFQCLQFYLYNSGS
  ESDQLNIYIREYSADNVDGNLTLVEEIKEIPTGSWQLYHVTLKVTKKFRVVFEGRKGSGASLGGLSIDDI
  NLSETRCPHHIWHIRNFTQFIGSPNGTLYSPPFYSSKGYAFQIYLNLAHVTNAGIYFHLISGANDDQLQW
  PCPWQQATMTLLDQNPDIRQRMSNQRSITTDPFMTTDNGNYFWDRPSKVGTVALFSNGTQFRRGGGYGTS
  AFITHERLKSRDFIKGDDVYILLTVEDISHLNSTQIQLTPAPSVQDLCSKTTCKNDGVCTVRDGKAECRC
  QSGEDWWYMGERCEKRGSTRDTIVIAVSSTVAVFALMLIITLVSVYCTRKKYRERMSSNRPNLTPQNQHA
  F
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005925
• ORF Size: 2103 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005925.3
• RefSeq Size: 2327 bp
• RefSeq ORF: 2106 bp
• Locus ID: 4225
• UniProt ID: Q16820
• Cytogenetics: 18q12.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 79.4 kDa
• Gene Summary: Meprins are multidomain zinc metalloproteases that are highly expressed in mammalian kidney and intestinal brush border membranes, and in leukocytes and certain cancer cells. They are involved in the hydrolysis of a variety of peptide and protein substrates, and have been implicated in cancer and intestinal inflammation. Mature meprins are oligomers of evolutionarily related, but separately encoded alpha and/or beta subunits. Homooligomers of alpha subunit are secreted, whereas, oligomers containing the beta subunit are plasma membrane-bound. This gene encodes the beta subunit. Targeted disruption of this gene in mice affects embryonic viability, renal gene expression profiles, and distribution of the membrane-associated alpha subunit in kidney and intestine. [provided by RefSeq, Oct 2011]