NPTX1 (NM_002522) Human Untagged Clone

CAT#: SC303211

NPTX1 (untagged)-Human neuronal pentraxin I (NPTX1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NPTX1
• Synonyms: NP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303211 representing NM_002522.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCCGGCCGGCCGCGCCGCGCGCACCTGTGCGCTGCTCGCCCTCTGCCTCCTGGGCGCCGGGGCCCAG
  GATTTCGGGCCGACGCGCTTCATCTGCACCTCGGTGCCCGTGGACGCCGACATGTGCGCCGCGTCCGTG
  GCCGCCGGCGGCGCCGAGGAGCTCCGGAGCAGCGTGCTGCAGCTCCGCGAGACGGTGCTGCAGCAGAAG
  GAGACCATCCTGAGCCAGAAGGAGACCATCCGCGAGCTGACCGCCAAGCTGGGCCGCTGCGAGAGCCAG
  AGCACGCTGGACCCCGGAGCCGGCGAGGCCCGGGCGGGCGGCGGCCGCAAGCAGCCCGGCTCGGGCAAG
  AACACCATGGGCGACCTGTCCCGGACACCGGCCGCCGAGACGCTCAGCCAACTCGGGCAAACTTTGCAA
  TCGCTCAAAACCCGCCTGGAGAACCTCGAGCAGTACAGCCGCCTCAATTCCTCCAGCCAGACCAACAGC
  CTCAAGGATCTGCTGCAGAGCAAGATCGATGAGCTGGAGAGGCAGGTGCTGTCCCGGGTGAACACCCTG
  GAGGAGGGCAAGGGGGGCCCCAGGAACGACACCGAGGAGAGGGTCAAGATCGAGACCGCCCTGACCTCC
  CTGCACCAGCGGATCAGCGAGCTCGAGAAAGGTCAGAAAGACAACCGCCCTGGAGACAAGTTCCAGCTC
  ACATTCCCACTGCGGACCAACTATATGTATGCCAAGGTGAAGAAGAGCCTGCCAGAGATGTACGCCTTC
  ACTGTCTGCATGTGGCTCAAGTCCAGCGCCACGCCAGGTGTGGGCACGCCCTTCTCCTACGCTGTGCCC
  GGCCAGGCCAACGAGCTGGTCCTCATTGAGTGGGGCAACAACCCCATGGAGATCCTCATCAATGACAAG
  GTGGCCAAGTTGCCTTTTGTCATCAATGATGGCAAGTGGCACCACATCTGTGTCACCTGGACCACCCGG
  GACGGGGTCTGGGAGGCCTACCAGGATGGCACGCAGGGTGGCAGTGGCGAGAACTTGGCGCCCTATCAC
  CCCATCAAGCCCCAGGGCGTGCTGGTGCTGGGCCAGGAGCAGGACACTCTGGGTGGTGGGTTTGATGCC
  ACCCAGGCATTTGTGGGTGAGCTGGCCCACTTCAACATCTGGGACCGCAAGCTGACCCCCGGGGAGGTG
  TACAACCTGGCCACCTGCAGCACCAAGGCTCTGTCCGGCAATGTCATCGCCTGGGCTGAATCCCACATC
  GAGATCTACGGAGGGGCCACCAAGTGGACCTTCGAGGCCTGTCGCCAGATCAACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002522
• Insert Size: 1299 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002522.3
• RefSeq Size: 5437 bp
• RefSeq ORF: 1299 bp
• Locus ID: 4884
• UniProt ID: Q15818
• Cytogenetics: 17q25.3
• Protein Families: Secreted Protein
• MW: 47.1 kDa
• Gene Summary: NPTX1 is a member of the neuronal pentraxin gene family. Neuronal pentraxin 1 is similar to the rat NP1 gene which encodes a binding protein for the snake venom toxin taipoxin. Human NPTX1 mRNA is exclusively localized to the nervous system. [provided by RefSeq, Jul 2008]