NG2 (CSPG4) (NM_001897) Human Untagged Clone

CAT#: SC303093

CSPG4 (untagged)-Human chondroitin sulfate proteoglycan 4 (CSPG4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NG2
• Synonyms: CSPG4A; HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001897 edited
  GAATTCATGCAGTCCGGGCCGCGGCCCCCACTTCCAGCCCCCGGCCTGGCCTTGGCTTTG
  ACCCTGACTATGTTGGCCAGACTTGCATCCGCGGCTTCCTTCTTCGGTGAGAACCACCTG
  GAGGTGCCTGTGGCCACGGCTCTGACCGACATAGACCTGCAGCTGCAGTTCTCCACGTCC
  CAGCCCGAAGCCCTCCTTCTCCTGGCAGCAGGCCCAGCTGACCACCTCCTGCTGCAGCTC
  TACTCTGGACGCCTGCAGGTCAGACTTGTTCTGGGCCAGGAGGAGCTGAGGCTGCAGACT
  CCAGCAGAGACGCTGCTGAGTGACTCCATCCCCCACACTGTGGTGCTGACTGTCGTAGAG
  GGCTGGGCCACGTTGTCAGTCGATGGGTTTCTGAACGCCTCCTCAGCAGTCCCAGGAGCC
  CCCCTAGAGGTCCCCTATGGGCTCTTTGTTGGGGGCACTGGGACCCTTGGCCTGCCCTAC
  CTGAGGGGAACCAGCCGACCCCTGAGGGGTTGCCTCCATGCAGCCACCCTCAATGGCCGC
  AGCCTCCTCCGGCCTCTGACCCCCGATGTGCATGAGGGCTGTGCTGAAGAGTTTTCTGCC
  AGTGATGATGTGGCCCTGGGCTTCTCTGGGCCCCACTCTCTGGCTGCCTTCCCTGCCTGG
  GGCACTCAGGACGAAGGAACCCTAGAGTTTACACTCACCACACAGAGCCGGCAGGCACCC
  TTGGCCTTCCAGGCAGGGGGCCGGCGTGGGGACTTCATCTATGTGGACATATTTGAGGGC
  CACCTGCGGGCCGTGGTGGAGAAGGGCCAGGGTACCGTATTGCTCCACAACAGTGTGCCT
  GTGGCCGATGGGCAGCCCCATGAGGTCAGTGTCCACATCAATGCTCACCGGCTGGAAATC
  TCCGTGGACCAGTACCCTACGCATACTTCGAACCGAGGAGTCCTCAGCTACCTGGAGCCA
  CGGGGCAGTCTCCTTCTCGGGGGGCTGGATGCAGAGGCCTCTCGTCACCTCCAGGAACAC
  CGCCTGGGCCTGACACCAGAGGCCACCAATGCCTCCCTGCTGGGCTGCATGGAAGACCTC
  AGTGTCAATGGCCAGAGGCGGGGGCTGCGGGAAGCTTTGCTGACGCGCAACATGGCAGCC
  GGCTGCAGGCTGGAGGAGGAGGAGTATGAGGACGATGCCTATGGACATTATGAAGCTTTC
  TCCACCCTGGCCCCTGAGGCTTGGCCAGCCATGGAGCTGCCTGAGCCATGCGTGCCTGAG
  CCAGGGCTGCCTCCTGTCTTTGCCAATTTCACCCAGCTGCTGACTATCAGCCCACTGGTG
  GTGGCCGAGGGGGGCACAGCCTGGCTTGAGTGGAGGCATGTGCAGCCCACGCTGGACCTG
  ATGGAGGCTGAGCTGCGCAAATCCCAGGTGCTGTTCAGCGTGACCCGAGGGGCACGCCAT
  GGCGAGCTCGAGCTGGACATCCCGGGAGCCCAGGCACGAAAAATGTTCACCCTCCTGGAC
  GTGGTGAACCGCAAGGCCCGCTTCATCCACGATGGCTCTGAGGACACCTCCGACCAGCTG
  GTGCTGGAGGTGTCGGTGACGGCTCGGGTGCCCATGCCCTCATGCCTTCGGAGGGGCCAA
  ACATACCTCCTGCCCATCCAGGTCAACCCTGTCAATGACCCACCCCACATCATCTTCCCA
  CATGGCAGCCTCATGGTGATCCTGGAACACACGCAGAAGCCGCTGGGGCCTGAGGTTTTC
  CAGGCCTATGACCCGGACTCTGCCTGTGAGGGCCTCACCTTCCAGGTCCTTGGCACCTCC
  TCTGGCCTCCCCGTGGAGCGCCGAGACCAGCCTGGGGAGCCGGCGACCGAGTTCTCCTGC
  CGGGAGTTGGAGGCCGGCAGCCTAGTCTATGTCCACCGCGGTGGTCCTGCACAGGACTTG
  ACGTTCCGGGTCAGCGATGGACTGCAGGCCAGCCCCCCGGCCACGCTGAAGGTGGTGGCC
  ATCCGGCCGGCCATACAGATCCACCGCAGCACAGGGTTGCGACTGGCCCAAGGCTCTGCC
  ATGCCCATCTTGCCCGCCAACCTGTCGGTGGAGACCAATGCCGTGGGGCAGGATGTGAGC
  GTGCTGTTCCGCGTCACTGGGGCCCTGCAGTTTGGGGAGCTGCAGAAGCAGGGGGCAGGT
  GGGGTGGAGGGTGCTGAGTGGTGGGCCACACAGGCGTTCCACCAGCGGGATGTGGAGCAG
  GGCCGCGTGAGGTACCTGAGCACTGACCCACAGCACCACGCTTACGACACCGTGGAGAAC
  CTGGCCCTGGAGGTGCAGGTGGGCCAGGAGATCCTGAGCAATCTGTCCTTCCCAGTGACC
  ATCCAGAGAGCCACTGTGTGGATGCTGCGGCTGGAGCCACTGCACACTCAGAACACCCAG
  CAGGAGACCCTCACCACAGCCCACCTGGAGGCCACCCTGGAGGAGGCAGGCCCAAGCCCC
  CCAACCTTCCATTATGAGGTGGTTCAGGCTCCCAGGAAAGGCAACCTTCAACTACAGGGC
  ACAAGGCTGTCAGATGGCCAGGGCTTCACCCAGGATGACATACAGGCTGGCCGGGTGACC
  TATGGGGCCACAGCACGTGCCTCAGAGGCAGTCGAGGACACCTTCCGTTTCCGTGTCACA
  GCTCCACCATATTTCTCCCCACTCTATACCTTCCCCATCCACATTGGTGGTGACCCAGAT
  GCGCCTGTCCTCACCAATGTCCTCCTCGTGGTGCCTGAGGGTGGTGAGGGTGTCCTCTCT
  GCTGACCACCTCTTTGTCAAGAGTCTCAACAGTGCCAGCTACCTCTATGAGGTCATGGAG
  CGGCCCCGCCATGGGAGGTTGGCTTGGCGTGGGACACAGGACAAGACCACTATGGTGACA
  TCCTTCACCAATGAAGACCTGTTGCGTGGCCGGCTGGTCTACCAGCATGATGACTCCGAG
  ACCACAGAAGATGATATCCCATTTGTTGCTACCCGCCAGGGCGAGAGCAGTGGTGACATG
  GCCTGGGAGGAGGTACGGGGTGTCTTCCGAGTGGCCATCCAGCCCGTGAATGACCACGCC
  CCTGTGCAGACCATCAGCCGGATCTTCCATGTGGCCCGGGGTGGGCGGCGGCTGCTGACT
  ACAGACGACGTGGCCTTCAGCGATGCTGACTCGGGCTTTGCTGACGCCCAGCTGGTGCTT
  ACCCGCAAGGACCTCCTCTTTGGCAGTATCGTGGCCGTAGATGAGCCCACGCGGCCCATC
  TACCGCTTCACCCAGGAGGACCTCAGGAAGAGGCGAGTACTGTTCGTGCACTCAGGGGCT
  GACCGTGGCTGGATCCAGCTGCAGGTGTCCGACGGGCAACACCAGGCCACTGCGCTGCTG
  GAGGTGCAGGCCTCGGAACCCTACCTCCGTGTGGCCAACGGCTCCAGCCTTGTGGTCCCT
  CAAGGAGGCCAGGGCACCATCGACACGGCCGTGCTCCACCTGGACACCAACCTCGACATC
  CGCAGTGGGGATGAGGTCCACTACCACGTCACAGCTGGCCCTCGCTGGGGACAGCTAGTC
  CGGGCTGGTCAGCCAGCCACAGCCTTCTCCCAGCAGGACCTGCTGGATGGGGCCGTTCTC
  TATAGCCACAATGGCAGCCTCAGCCCCCGAGACACCATGGCCTTCTCCGTGGAAGCAGGG
  CCAGTGCACACGGATGCCACCCTACAAGTGACCATTGCCCTAGAGGGCCCACTGGCCCCA
  CTGAAGCTGGTCCGGCACAAGAAGATCTACGTCTTCCAGGGAGAGGCAGCTGAGATCAGA
  AGGGACCAGCTGGAGGCAGCCCAGGAGGCAGTGCCACCTGCAGACATCGTATTCTCAGTG
  AAGAGCCCACCGAGTGCCGGCTACCTGGTGATGGTGTCGCGTGGCGCCTTGGCAGATGAG
  CCACCCAGCCTGGACCCCGTGCAGAGCTTCTCCCAGGAGGCAGTGGACACAGGCAGGGTC
  CTGTACCTGCACTCCCGCCCTGAGGCCTGGAGCGATGCCTTCTCGCTGGATGTGGCCTCA
  GGCCTGGGTGCTCCCCTCGAGGGCGTCCTTGTGGAGCTGGAGGTGCTGCCCGCTGCCATC
  CCACTAGAGGCGCAAAACTTCAGCGTCCCTGAGGGTGGCAGCCTCACCCTGGCCCCTCCA
  CTGCTCCGTGTCTCCGGGCCCTACTTCCCCACTCTCCTGGGCCTCAGCCTGCAGGTGCTG
  GAGCCACCCCAGCATGGAGCCCTGCAGAAGGAGGACGGACCTCAAGCCAGGACCCTCAGC
  GCCTTCTCCTGGAGAATGGTGGAAGAGCAGCTGATCCGCTACGTGCATGACGGGAGCGAG
  ACACTGACAGACAGTTTTGTCCTGATGGCTAATGCCTCCGAGATGGATCGCCAGAGCCAT
  CCTGTGGCCTTCACTGTCACTGTCCTGCCTGTCAATGACCAACCCCCCATCCTCACTACA
  AACACAGGCCTGCAGATGTGGGAGGGGGCCACTGCGCCCATCCCTGCGGAGGCTCTGAGG
  AGCACGGACGGCGACTCTGGGTCTGAGGATCTGGTCTACACCATCGAGCAGCCCAGCAAC
  GGGCGGGTAGTGCTGCGGGGGGCGCCGGGCACTGAGGTGCGCAGCTTCACGCAGGCCCAG
  CTGGACGGCGGGCTCGTGCTGTTCTCACACAGAGGAACCCTGGATGGAGGCTTCCGCTTC
  CGCCTCTCTGACGGCGAGCACACTTCCCCCGGACACTTCTTCCGAGTGACGGCCCAGAAG
  CAAGTGCTCCTCTCGCTGAAGGGCAGCCAGACACTGACTGTCTGCCCAGGGTCCGTCCAG
  CCACTCAGCAGTCAGACCCTCAGGGCCAGCTCCAGCGCAGGCACTGACCCCCAGCTCCTG
  CTCTACCGTGTGGTGCGGGGCCCCCAGCTAGGCCGGCTGTTCCACGCCCAGCAGGACAGC
  ACAGGGGAGGCCCTGGTGAACTTCACTCAGGCAGAGGTCTACGCTGGGAATATTCTGTAT
  GAGCATGAGATGCCCCCCGAGCCCTTTTGGGAGGCCCATGATACCCTAGAGCTCCAGCTG
  TCCTCGCCGCCTGCCCGGGACGTGGCCGCCACCCTTGCTGTGGCTGTGTCTTTTGAGGCT
  GCCTGTCCCCAGCACCCCAGCCACCTCTGGAAGAACAAAGGTCTCTGGGTCCCCGAGGGC
  CAGCGGGCCAGGATCACCGTGGCTGCTCTGGATGCCTCCAATCTCTTGGCCAGCGTTCCA
  TCACCCCAGCGCTCAGAGCATGATGTGCTCTTCCAGGTCACACAGTTCCCCAGCCGGGGC
  CAGCTGTTGGTGTCCGAGGAGCCCCTCCATGCTGGGCAGCCCCACTTCCTGCAGTCCCAG
  CTGGCTGCAGGGCAGCTAGTGTATGCCCACGGCGGTGGGGGCACCCAGCAGGATGGCTTC
  CACTTTCGTGCCCACCTCCAGGGGCCAGCAGGGGCCTCCGTGGCTGGACCCCAAACCTCA
  GAGGCCTTTGCCATCACGGTGAGGGATGTAAATGAGCGGCCCCCTCAGCCACAGGCCTCT
  GTCCCACTCCGGCTCACCCGAGGCTCTCGTGCCCCCATCTCCCGGGCCCAGCTGAGTGTG
  GTGGACCCAGACTCAGCTCCTGGGGAGATTGAGTACGAGGTCCAGCGGGCACCCCACAAC
  GGCTTCCTCAGCCTGGTGGGTGGTGGCCTGGGGCCCGTGACCCGCTTCACGCAAGCCGAT
  GTGGATTCAGGGCGGCTGGCCTTCGTGGCCAACGGGAGCAGCGTGGCAGGCATCTTCCAG
  CTGAGCATGTCTGATGGGGCCAGCCCACCCCTGCCCATGTCCCTGGCTGTGGACATCCTA
  CCATCCGCCATCGAGGTGCAGCTGCGGGCACCCCTGGAGGTGCCCCAAGCTTTGGGGCGC
  TCCTCACTGAGCCAGCAGCAGCTCCGGGTGGTTTCAGATCGGGAGGAGCCAGAGGCAGCA
  TACCGCCTCATCCAGGGACCCCAGTATGGGCATCTCCTGGTGGGCGGGCGGCCCACCTCG
  GCCTTCAGCCAATTCCAGATAGACCAGGGCGAGGTGGTCTTTGCCTTCACCAACTTCTCC
  TCCTCTCATGACCACTTCAGAGTCCTGGCACTGGCTAGGGGTGTCAATGCATCAGCCGTA
  GTGAACGTCACTGTGAGGGCTCTGCTGCATGTGTGGGCAGGTGGGCCATGGCCCCAGGGT
  GCCACCCTGCGCCTGGACCCCACCGTCCTAGATGCTGGCGAGCTGGCCAACCGCACAGGC
  AGTGTGCCGCGCTTCCGCCTCCTGGAGGGACCCCGGCATGGCCGCGTGGTCCGCGTGCCC
  CGAGCCAGGACGGAGCCCGGGGGCAGCCAGCTGGTGGAGCAGTTCACTCAGCAGGACCTT
  GAGGACGGGAGGCTGGGGCTGGAGGTGGGCAGGCCAGAGGGGAGGGCCCCCGGCCCCGCA
  GGTGACAGTCTCACTCTGGAGCTGTGGGCACAGGGCGTCCCGCCTGCTGTGGCCTCCCTG
  GACTTTGCCACTGAGCCTTACAATGCTGCCCGGCCCTACAGCGTGGCCCTGCTCAGTGTC
  CCCGAGGCCGCCCGGACGGAAGCAGGGAAGCCAGAGAGCAGCACCCCCACAGGCGAGCCA
  GGCCCCATGGCATCCAGCCCTGAGCCCGCTGTGGCCAAGGGAGGCTTCCTGAGCTTCCTT
  GAGGCCAACATGTTCAGCGTCATCATCCCCATGTGCCTGGTACTTCTGCTCCTGGCGCTC
  ATCCTGCCCCTGCTCTTCTACCTCCGAAAACGCAACAAGACGGGCAAGCATGACGTCCAG
  GTCCTGACTGCCAAGCCCCGCAACGGCCTGGCTGGTGACACCGAGACCTTTCGCAAGGTG
  GAGCCAGGCCAGGCCATCCCGCTCACAGCTGTGCCTGGCCAGGGGCCCCCTCCAGGAGGC
  CAGCCTGACCCAGAGCTGCTGCAGTTCTGCCGGACACCCAACCCTGCCCTTAAGAATGGC
  CAGTACTGGGTGTGAGGCCTGGCCTGGGCCCAGATGCTGATCGGGCCAGGGACAGGCTTG
  CCCATGTCCCGGGCCCCATTGCTTCCATGCCTGGTGCTGTCTGAGTATCCCCAGAGCAAG
  AGAGACCTGGAGACACCAGGGGTGGAGGGTCCTGGGAGATAGTCCCAGGGGTCCGGGACA
  GAGTGGAGTCAAGAGCTGGAACCTCCCTCAGCTCACTCCGAGCCTGGAGAACTGCAGGGG
  CCAAGGTGGAGGCAGGCTTAAGTTCAGTCCTCCTGCCCTGGAGCTGGTTTGGGCTGTCAA
  AACCAGGGTAACCTCCTACATGGGTCATGACTCTGGGTCCTGGGTCTGTGACCTTGGGTA
  AGTCGCGCCTGACCCAGGCTGCTAAGAGGGCAAGGAGAAGGAAGTACCCTGGGGAGGGAA
  GGGACAGAGGAAGCTATTCCTGGCTTTTCCACTCCAACCCAGGCCACCCTTTGTCTCTGC
  CCCAGAGTTGAGAAAAAAAACTTCCTCCCCTGGTTTTTTAGGGAGATGGTATCCCCTGGA
  GTAGAGGGCAAGAGGAGAGAGCGCCTCCAGTCTAGAAGGCATAAGCCAATAGGATAATAT
  ATTCAGGGTGCAGGGTGGGTAGGTTGCTCTGGGGATGGGTTTATTTAAGGGAGATTGCAA
  GGAAGCTATTTAACATGGTGCTGAGCTAGCCAGGACTGATGGAGCCCCTGGGGGTGTGGG
  ATGGAGGAGGGTCTGCAGCCAGTTCATTCCCAGGGCCCCATCTTGATGGGCCAAGGGCTA
  AACATGCATGTGTCAGTGGCTTTGGAGCAGGTTAGGCTGGGGCTCATCGAGGGTCTCAGG
  CCGAGGCCACTGCGGTGCCAGTGCCCCCCTGAGGACTAGGGCAGGCAGCTGGGGGCACTT
  GGTTCCATGGAGCCTGGATAAACAGTGCTTTGGAGGCTCTGGAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001897 unedited
  GGTAACTGATTTGTATACGACTCCTATAGGGCGGCCGCGATATTCATGCAGTCCGGGCCG
  CGGCCCCCACTTCCAGCCCCCGGCCTGGCCTTGGCTTTGACCCTGACTATGTTGGCCAGA
  CTTGCATCCGCGGCTTCCTTCTTCGGTGAGAACCACCTGGAGGTGCCTGTGGCCACGGCT
  CTGACCGACATAGACCTGCAGCTGCAGTTCTCCACGTCCCAGCCCGAAGCCCTCCTTCTC
  CTGGCAGCAGGCCCAGCTGACCACCTCCTGCTGCAGCTCTACTCTGGACGCCTGCAGGTC
  AGACTTGTTCTGGGCCAGGAGGAGCTGAGGCTGCAGACTCCAGCAGAGACGCTGCTGAGT
  GACTCCATCCCCCACACTGTGGTGCTGACTGTCGTAGAGGGCTGGGCCACGTTGTCAGTC
  GATGGGTTTCTGAACGCCTCCTCAGCAGTCCCAGGAGCCCCCCTAGAGGTCCCCTATGGG
  CTCTTTGTTGGGGGCACTGGGACCCTTGGCCTGCCCTACCTGAGGGGAACCAGCCGACCC
  CTGAGGGGTTGCCTCCATGCAGCCACCCTCAATGGCCGCAGCCTCCTCCGGCCTCTGACC
  CCCGATGTGCATGAGGGCTGTGCTGAAGAGTTTTCTGCCAGTGATGATGTGGCCCTGGGC
  TTCTCTGGGCCCCACTCTCTGGCTGCCTTCCCTGCCTGGGGCACTCAGGACGAAGGAACC
  CTAGAGTTTACACTCACCACACAGAGCCCGGCAGGCACCCTTGGCCTTCCAGGCAGGGGC
  CGGCGTGGGGACTTCATCTATGTGGACATATTTGAGGGCCACCTGCGGGCCGTGGTGGAG
  AAGGCCAGGGTACCGTATTGCTCACAACAGTGTGCCTGTGGCCGATGGGCAGCCCATGAG
  TCAGTGTCACATCAATGCTCACCGGCTGGAAATCTTCCGTGGAACCAGTACCTACG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001897 unedited
  CGCAGATGCACTTCAGGGCCGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG
  ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTCCAGAGCCTCCAAAGCACTGTTTATCCAGGCTCCATGGAACC
  AAGTGCCCCCAGCTGCCTGCCCTAGTCCTCAGGGGGGCACTGGCACCGCAGTGGCCTCGG
  CCTGAGACCCTCGATGAGCCCCAGCCTAACCTGCTCCAAAGCCACTGACACATGCATGTT
  TAGCCCTTGGCCCATCAAGATGGGGCCCTGGGAATGAACTGGCTGCAAACCCTCCTCCAT
  CCCACACCCCCAGGGGCTCCATCAGTCCTGGCTAGCTCAGCACCATGTTAAATAGCTTCC
  TTGCAATCTCCCTTAAATAAACCCATCCCCAGAGCAACCTACCCACCCTGCACCCTGAAT
  ATATTATCCTATTGGCTTATGCCTTCTAGACTGGAGGCGCTCTCTCCTCTTGCCCTCTAC
  TCCAGGGGATACCATCTCCCTAAAAAACCAGGGGAGGAAGTTTTTTTTCTCAACTCTGGG
  GCAGAGACAAAGGGTGGCCTGGGTTGGAGTGGAAAAGCCAGGAATAGCTTCCTCTGTCCC
  TTCCCTCCCCAGGGTACTTCCTTCTCCTTGCCCTCTTAGCAGCCTGGGTCAGGCGCGACT
  TACCCAAGGTCACAGACCCAGGACCCAGAGTCATGACCCATGTAGAGGTTACCCTGGTTT
  TGACAGCCCAAACCAGCTCAGGGCAGGAGGACTGAACTTAGCCTGCCTCACCTTGCCTGC
  AGTTCTCAGCTCGGAGTGAGCTGAGGGAGGTTCAGCTCTTGACTCCACTCTGTCCCGGAC
  CCTGGGACTAT
  
• Restriction Sites: Please inquire
• ACCN: NM_001897
• Insert Size: 8000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There is 1 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 1 aa and insertion of 1 aa.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001897.3, NP_001888.2
• RefSeq Size: 7896 bp
• RefSeq ORF: 6969 bp
• Locus ID: 1464
• UniProt ID: Q6UVK1
• Cytogenetics: 15q24.2
• Protein Families: Transmembrane
• Gene Summary: A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008]