NG2(CSPG4) (NM_001897) Human Tagged ORF Clone

CAT#: RC218462

CSPG4 (Myc-DDK-tagged)-Human chondroitin sulfate proteoglycan 4 (CSPG4)

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Size
    • 10 ug

Cited in 1 publication.

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Specifications

Product Data
Product Name NG2(CSPG4) (NM_001897) Human Tagged ORF Clone
Symbol CSPG4
Synonyms HMW-MAA; MCSP; MCSPG; MEL-CSPG; MSK16; NG2
Vector pCMV6-Entry
Sequence Data
>RC218462 representing NM_001897
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGTCCGGGCCGCGGCCCCCACTTCCAGCCCCCGGCCTGGCCTTGGCTTTGACCCTGACTATGTTGG
CCAGACTTGCATCCGCGGCTTCCTTCTTCGGTGAGAACCACCTGGAGGTGCCTGTGGCCACGGCTCTGAC
CGACATAGACCTGCAGCTGCAGTTCTCCACGTCCCAGCCCGAAGCCCTCCTTCTCCTGGCAGCAGGCCCA
GCTGACCACCTCCTGCTGCAGCTCTACTCTGGACGCCTGCAGGTCAGACTTGTTCTGGGCCAGGAGGAGC
TGAGGCTGCAGACTCCAGCAGAGACGCTGCTGAGTGACTCCATCCCCCACACTGTGGTGCTGACTGTCGT
AGAGGGCTGGGCCACGTTGTCAGTCGATGGGTTTCTGAACGCCTCCTCAGCAGTCCCAGGAGCCCCCCTA
GAGGTCCCCTATGGGCTCTTTGTTGGGGGCACTGGGACCCTTGGCCTGCCCTACCTGAGGGGAACCAGCC
GACCCCTGAGGGGTTGCCTCCATGCAGCCACCCTCAATGGCCGCAGCCTCCTCCGGCCTCTGACCCCCGA
TGTGCATGAGGGCTGTGCTGAAGAGTTTTCTGCCAGTGATGATGTGGCCCTGGGCTTCTCTGGGCCCCAC
TCTCTGGCTGCCTTCCCTGCCTGGGGCACTCAGGACGAAGGAACCCTAGAGTTTACACTCACCACACAGA
GCCGGCAGGCACCCTTGGCCTTCCAGGCAGGGGGCCGGCGTGGGGACTTCATCTATGTGGACATATTTGA
GGGCCACCTGCGGGCCGTGGTGGAGAAGGGCCAGGGTACCGTATTGCTCCACAACAGTGTGCCTGTGGCC
GATGGGCAGCCCCATGAGGTCAGTGTCCACATCAATGCTCACCGGCTGGAAATCTCCGTGGACCAGTACC
CTACGCATACTTCGAACCGAGGAGTCCTCAGCTACCTGGAGCCACGGGGCAGTCTCCTTCTCGGGGGGCT
GGATGCAGAGGCCTCTCGTCACCTCCAGGAACACCGCCTGGGCCTGACACCAGAGGCCACCAATGCCTCC
CTGCTGGGCTGCATGGAAGACCTCAGTGTCAATGGCCAGAGGCGGGGGCTGCGGGAAGCTTTGCTGACGC
GCAACATGGCAGCCGGCTGCAGGCTGGAGGAGGAGGAGTATGAGGACGATGCCTATGGACATTATGAAGC
TTTCTCCACCCTGGCCCCTGAGGCTTGGCCAGCCATGGAGCTGCCTGAGCCATGCGTGCCTGAGCCAGGG
CTGCCTCCTGTCTTTGCCAATTTCACCCAGCTGCTGACTATCAGCCCACTGGTGGTGGCCGAGGGGGGCA
CAGCCTGGCTTGAGTGGAGGCATGTGCAGCCCACGCTGGACCTGATGGAGGCTGAGCTGCGCAAATCCCA
GGTGCTGTTCAGCGTGACCCGAGGGGCACGCCATGGCGAGCTCGAGCTGGACATCCCGGGAGCCCAGGCA
CGAAAAATGTTCACCCTCCTGGACGTGGTGAACCGCAAGGCCCGCTTCATCCACGATGGCTCTGAGGACA
CCTCCGACCAGCTGGTGCTGGAGGTGTCGGTGACGGCTCGGGTGCCCATGCCCTCATGCCTTCGGAGGGG
CCAAACATACCTCCTGCCCATCCAGGTCAACCCTGTCAATGACCCACCCCACATCATCTTCCCACATGGC
AGCCTCATGGTGATCCTGGAACACACGCAGAAGCCGCTGGGGCCTGAGGTTTTCCAGGCCTATGACCCGG
ACTCTGCCTGTGAGGGCCTCACCTTCCAGGTCCTTGGCACCTCCTCTGGCCTCCCCGTGGAGCGCCGAGA
CCAGCCTGGGGAGCCGGCGACCGAGTTCTCCTGCCGGGAGTTGGAGGCCGGCAGCCTAGTCTATGTCCAC
CGCGGTGGTCCTGCACAGGACTTGACGTTCCGGGTCAGCGATGGACTGCAGGCCAGCCCCCCGGCCACGC
TGAAGGTGGTGGCCATCCGGCCGGCCATACAGATCCACCGCAGCACAGGGTTGCGACTGGCCCAAGGCTC
TGCCATGCCCATCTTGCCCGCCAACCTGTCGGTGGAGACCAATGCCGTGGGGCAGGATGTGAGCGTGCTG
TTCCGCGTCACTGGGGCCCTGCAGTTTGGGGAGCTGCAGAAGCAGGGGGCAGGTGGGGTGGAGGGTGCTG
AGTGGTGGGCCACACAGGCGTTCCACCAGCGGGATGTGGAGCAGGGCCGCGTGAGGTACCTGAGCACTGA
CCCACAGCACCACGCTTACGACACCGTGGAGAACCTGGCCCTGGAGGTGCAGGTGGGCCAGGAGATCCTG
AGCAATCTGTCCTTCCCAGTGACCATCCAGAGAGCCACTGTGTGGATGCTGCGGCTGGAGCCACTGCACA
CTCAGAACACCCAGCAGGAGACCCTCACCACAGCCCACCTGGAGGCCACCCTGGAGGAGGCAGGCCCAAG
CCCCCCAACCTTCCATTATGAGGTGGTTCAGGCTCCCAGGAAAGGCAACCTTCAACTACAGGGCACAAGG
CTGTCAGATGGCCAGGGCTTCACCCAGGATGACATACAGGCTGGCCGGGTGACCTATGGGGCCACAGCAC
GTGCCTCAGAGGCAGTCGAGGACACCTTCCGTTTCCGTGTCACAGCTCCACCATATTTCTCCCCACTCTA
TACCTTCCCCATCCACATTGGTGGTGACCCAGATGCGCCTGTCCTCACCAATGTCCTCCTCGTGGTGCCT
GAGGGTGGTGAGGGTGTCCTCTCTGCTGACCACCTCTTTGTCAAGAGTCTCAACAGTGCCAGCTACCTCT
ATGAGGTCATGGAGCGGCCCCGCCATGGGAGGTTGGCTTGGCGTGGGACACAGGACAAGACCACTATGGT
GACATCCTTCACCAATGAAGACCTGTTGCGTGGCCGGCTGGTCTACCAGCATGATGACTCCGAGACCACA
GAAGATGATATCCCATTTGTTGCTACCCGCCAGGGCGAGAGCAGTGGTGACATGGCCTGGGAGGAGGTAC
GGGGTGTCTTCCGAGTGGCCATCCAGCCCGTGAATGACCACGCCCCTGTGCAGACCATCAGCCGGATCTT
CCATGTGGCCCGGGGTGGGCGGCGGCTGCTGACTACAGACGACGTGGCCTTCAGCGATGCTGACTCGGGC
TTTGCTGACGCCCAGCTGGTGCTTACCCGCAAGGACCTCCTCTTTGGCAGTATCGTGGCCGTAGATGAGC
CCACGCGGCCCATCTACCGCTTCACCCAGGAGGACCTCAGGAAGAGGCGAGTACTGTTCGTGCACTCAGG
GGCTGACCGTGGCTGGATCCAGCTGCAGGTGTCCGACGGGCAACACCAGGCCACTGCGCTGCTGGAGGTG
CAGGCCTCGGAACCCTACCTCCGTGTGGCCAACGGCTCCAGCCTTGTGGTCCCTCAAGGAGGCCAGGGCA
CCATCGACACGGCCGTGCTCCACCTGGACACCAACCTCGACATCCGCAGTGGGGATGAGGTCCACTACCA
CGTCACAGCTGGCCCTCGCTGGGGACAGCTAGTCCGGGCTGGTCAGCCAGCCACAGCCTTCTCCCAGCAG
GACCTGCTGGATGGGGCCGTTCTCTATAGCCACAATGGCAGCCTCAGCCCCCGAGACACCATGGCCTTCT
CCGTGGAAGCAGGGCCAGTGCACACGGATGCCACCCTACAAGTGACCATTGCCCTAGAGGGCCCACTGGC
CCCACTGAAGCTGGTCCGGCACAAGAAGATCTACGTCTTCCAGGGAGAGGCAGCTGAGATCAGAAGGGAC
CAGCTGGAGGCAGCCCAGGAGGCAGTGCCACCTGCAGACATCGTATTCTCAGTGAAGAGCCCACCGAGTG
CCGGCTACCTGGTGATGGTGTCGCGTGGCGCCTTGGCAGATGAGCCACCCAGCCTGGACCCCGTGCAGAG
CTTCTCCCAGGAGGCAGTGGACACAGGCAGGGTCCTGTACCTGCACTCCCGCCCTGAGGCCTGGAGCGAT
GCCTTCTCGCTGGATGTGGCCTCAGGCCTGGGTGCTCCCCTCGAGGGCGTCCTTGTGGAGCTGGAGGTGC
TGCCCGCTGCCATCCCACTAGAGGCGCAAAACTTCAGCGTCCCTGAGGGTGGCAGCCTCACCCTGGCCCC
TCCACTGCTCCGTGTCTCCGGGCCCTACTTCCCCACTCTCCTGGGCCTCAGCCTGCAGGTGCTGGAGCCA
CCCCAGCATGGAGCCCTGCAGAAGGAGGACGGACCTCAAGCCAGGACCCTCAGCGCCTTCTCCTGGAGAA
TGGTGGAAGAGCAGCTGATCCGCTACGTGCATGACGGGAGCGAGACACTGACAGACAGTTTTGTCCTGAT
GGCTAATGCCTCCGAGATGGATCGCCAGAGCCATCCTGTGGCCTTCACTGTCACTGTCCTGCCTGTCAAT
GACCAACCCCCCATCCTCACTACAAACACAGGCCTGCAGATGTGGGAGGGGGCCACTGCGCCCATCCCTG
CGGAGGCTCTGAGGAGCACGGACGGCGACTCTGGGTCTGAGGATCTGGTCTACACCATCGAGCAGCCCAG
CAACGGGCGGGTAGTGCTGCGGGGGGCGCCGGGCACTGAGGTGCGCAGCTTCACGCAGGCCCAGCTGGAC
GGCGGGCTCGTGCTGTTCTCACACAGAGGAACCCTGGATGGAGGCTTCCGCTTCCGCCTCTCTGACGGCG
AGCACACTTCCCCCGGACACTTCTTCCGAGTGACGGCCCAGAAGCAAGTGCTCCTCTCGCTGAAGGGCAG
CCAGACACTGACTGTCTGCCCAGGGTCCGTCCAGCCACTCAGCAGTCAGACCCTCAGGGCCAGCTCCAGC
GCAGGCACTGACCCCCAGCTCCTGCTCTACCGTGTGGTGCGGGGCCCCCAGCTAGGCCGGCTGTTCCACG
CCCAGCAGGACAGCACAGGGGAGGCCCTGGTGAACTTCACTCAGGCAGAGGTCTACGCTGGGAATATTCT
GTATGAGCATGAGATGCCCCCCGAGCCCTTTTGGGAGGCCCATGATACCCTAGAGCTCCAGCTGTCCTCG
CCGCCTGCCCGGGACGTGGCCGCCACCCTTGCTGTGGCTGTGTCTTTTGAGGCTGCCTGTCCCCAGCACC
CCAGCCACCTCTGGAAGAACAAAGGTCTCTGGGTCCCCGAGGGCCAGCGGGCCAGGATCACCGTGGCTGC
TCTGGATGCCTCCAATCTCTTGGCCAGCGTTCCATCACCCCAGCGCTCAGAGCATGATGTGCTCTTCCAG
GTCACACAGTTCCCCAGCCGGGGCCAGCTGTTGGTGTCCGAGGAGCCCCTCCATGCTGGGCAGCCCCACT
TCCTGCAGTCCCAGCTGGCTGCAGGGCAGCTAGTGTATGCCCACGGCGGTGGGGGCACCCAGCAGGATGG
CTTCCACTTTCGTGCCCACCTCCAGGGGCCAGCAGGGGCCTCCGTGGCTGGACCCCAAACCTCAGAGGCC
TTTGCCATCACGGTGAGGGATGTAAATGAGCGGCCCCCTCAGCCACAGGCCTCTGTCCCACTCCGGCTCA
CCCGAGGCTCTCGTGCCCCCATCTCCCGGGCCCAGCTGAGTGTGGTGGACCCAGACTCAGCTCCTGGGGA
GATTGAGTACGAGGTCCAGCGGGCACCCCACAACGGCTTCCTCAGCCTGGTGGGTGGTGGCCTGGGGCCC
GTGACCCGCTTCACGCAAGCCGATGTGGATTCAGGGCGGCTGGCCTTCGTGGCCAACGGGAGCAGCGTGG
CAGGCATCTTCCAGCTGAGCATGTCTGATGGGGCCAGCCCACCCCTGCCCATGTCCCTGGCTGTGGACAT
CCTACCATCCGCCATCGAGGTGCAGCTGCGGGCACCCCTGGAGGTGCCCCAAGCTTTGGGGCGCTCCTCA
CTGAGCCAGCAGCAGCTCCGGGTGGTTTCAGATCGGGAGGAGCCAGAGGCAGCATACCGCCTCATCCAGG
GACCCCAGTATGGGCATCTCCTGGTGGGCGGGCGGCCCACCTCGGCCTTCAGCCAATTCCAGATAGACCA
GGGCGAGGTGGTCTTTGCCTTCACCAACTTCTCCTCCTCTCATGACCACTTCAGAGTCCTGGCACTGGCT
AGGGGTGTCAATGCATCAGCCGTAGTGAACGTCACTGTGAGGGCTCTGCTGCATGTGTGGGCAGGTGGGC
CATGGCCCCAGGGTGCCACCCTGCGCCTGGACCCCACCGTCCTAGATGCTGGCGAGCTGGCCAACCGCAC
AGGCAGTGTGCCGCGCTTCCGCCTCCTGGAGGGACCCCGGCATGGCCGCGTGGTCCGCGTGCCCCGAGCC
AGGACGGAGCCCGGGGGCAGCCAGCTGGTGGAGCAGTTCACTCAGCAGGACCTTGAGGACGGGAGGCTGG
GGCTGGAGGTGGGCAGGCCAGAGGGGAGGGCCCCCGGCCCCGCAGGTGACAGTCTCACTCTGGAGCTGTG
GGCACAGGGCGTCCCGCCTGCTGTGGCCTCCCTGGACTTTGCCACTGAGCCTTACAATGCTGCCCGGCCC
TACAGCGTGGCCCTGCTCAGTGTCCCCGAGGCCGCCCGGACGGAAGCAGGGAAGCCAGAGAGCAGCACCC
CCACAGGCGAGCCAGGCCCCATGGCATCCAGCCCTGAGCCCGCTGTGGCCAAGGGAGGCTTCCTGAGCTT
CCTTGAGGCCAACATGTTCAGCGTCATCATCCCCATGTGCCTGGTACTTCTGCTCCTGGCGCTCATCCTG
CCCCTGCTCTTCTACCTCCGAAAACGCAACAAGACGGGCAAGCATGACGTCCAGGTCCTGACTGCCAAGC
CCCGCAACGGCCTGGCTGGTGACACCGAGACCTTTCGCAAGGTGGAGCCAGGCCAGGCCATCCCGCTCAC
AGCTGTGCCTGGCCAGGGGCCCCCTCCAGGAGGCCAGCCTGACCCAGAGCTGCTGCAGTTCTGCCGGACA
CCCAACCCTGCCCTTAAGAATGGCCAGTACTGGGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218462 representing NM_001897
Red=Cloning site Green=Tags(s)

MQSGPRPPLPAPGLALALTLTMLARLASAASFFGENHLEVPVATALTDIDLQLQFSTSQPEALLLLAAGP
ADHLLLQLYSGRLQVRLVLGQEELRLQTPAETLLSDSIPHTVVLTVVEGWATLSVDGFLNASSAVPGAPL
EVPYGLFVGGTGTLGLPYLRGTSRPLRGCLHAATLNGRSLLRPLTPDVHEGCAEEFSASDDVALGFSGPH
SLAAFPAWGTQDEGTLEFTLTTQSRQAPLAFQAGGRRGDFIYVDIFEGHLRAVVEKGQGTVLLHNSVPVA
DGQPHEVSVHINAHRLEISVDQYPTHTSNRGVLSYLEPRGSLLLGGLDAEASRHLQEHRLGLTPEATNAS
LLGCMEDLSVNGQRRGLREALLTRNMAAGCRLEEEEYEDDAYGHYEAFSTLAPEAWPAMELPEPCVPEPG
LPPVFANFTQLLTISPLVVAEGGTAWLEWRHVQPTLDLMEAELRKSQVLFSVTRGARHGELELDIPGAQA
RKMFTLLDVVNRKARFIHDGSEDTSDQLVLEVSVTARVPMPSCLRRGQTYLLPIQVNPVNDPPHIIFPHG
SLMVILEHTQKPLGPEVFQAYDPDSACEGLTFQVLGTSSGLPVERRDQPGEPATEFSCRELEAGSLVYVH
RGGPAQDLTFRVSDGLQASPPATLKVVAIRPAIQIHRSTGLRLAQGSAMPILPANLSVETNAVGQDVSVL
FRVTGALQFGELQKQGAGGVEGAEWWATQAFHQRDVEQGRVRYLSTDPQHHAYDTVENLALEVQVGQEIL
SNLSFPVTIQRATVWMLRLEPLHTQNTQQETLTTAHLEATLEEAGPSPPTFHYEVVQAPRKGNLQLQGTR
LSDGQGFTQDDIQAGRVTYGATARASEAVEDTFRFRVTAPPYFSPLYTFPIHIGGDPDAPVLTNVLLVVP
EGGEGVLSADHLFVKSLNSASYLYEVMERPRHGRLAWRGTQDKTTMVTSFTNEDLLRGRLVYQHDDSETT
EDDIPFVATRQGESSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRIFHVARGGRRLLTTDDVAFSDADSG
FADAQLVLTRKDLLFGSIVAVDEPTRPIYRFTQEDLRKRRVLFVHSGADRGWIQLQVSDGQHQATALLEV
QASEPYLRVANGSSLVVPQGGQGTIDTAVLHLDTNLDIRSGDEVHYHVTAGPRWGQLVRAGQPATAFSQQ
DLLDGAVLYSHNGSLSPRDTMAFSVEAGPVHTDATLQVTIALEGPLAPLKLVRHKKIYVFQGEAAEIRRD
QLEAAQEAVPPADIVFSVKSPPSAGYLVMVSRGALADEPPSLDPVQSFSQEAVDTGRVLYLHSRPEAWSD
AFSLDVASGLGAPLEGVLVELEVLPAAIPLEAQNFSVPEGGSLTLAPPLLRVSGPYFPTLLGLSLQVLEP
PQHGALQKEDGPQARTLSAFSWRMVEEQLIRYVHDGSETLTDSFVLMANASEMDRQSHPVAFTVTVLPVN
DQPPILTTNTGLQMWEGATAPIPAEALRSTDGDSGSEDLVYTIEQPSNGRVVLRGAPGTEVRSFTQAQLD
GGLVLFSHRGTLDGGFRFRLSDGEHTSPGHFFRVTAQKQVLLSLKGSQTLTVCPGSVQPLSSQTLRASSS
AGTDPQLLLYRVVRGPQLGRLFHAQQDSTGEALVNFTQAEVYAGNILYEHEMPPEPFWEAHDTLELQLSS
PPARDVAATLAVAVSFEAACPQHPSHLWKNKGLWVPEGQRARITVAALDASNLLASVPSPQRSEHDVLFQ
VTQFPSRGQLLVSEEPLHAGQPHFLQSQLAAGQLVYAHGGGGTQQDGFHFRAHLQGPAGASVAGPQTSEA
FAITVRDVNERPPQPQASVPLRLTRGSRAPISRAQLSVVDPDSAPGEIEYEVQRAPHNGFLSLVGGGLGP
VTRFTQADVDSGRLAFVANGSSVAGIFQLSMSDGASPPLPMSLAVDILPSAIEVQLRAPLEVPQALGRSS
LSQQQLRVVSDREEPEAAYRLIQGPQYGHLLVGGRPTSAFSQFQIDQGEVVFAFTNFSSSHDHFRVLALA
RGVNASAVVNVTVRALLHVWAGGPWPQGATLRLDPTVLDAGELANRTGSVPRFRLLEGPRHGRVVRVPRA
RTEPGGSQLVEQFTQQDLEDGRLGLEVGRPEGRAPGPAGDSLTLELWAQGVPPAVASLDFATEPYNAARP
YSVALLSVPEAARTEAGKPESSTPTGEPGPMASSPEPAVAKGGFLSFLEANMFSVIIPMCLVLLLLALIL
PLLFYLRKRNKTGKHDVQVLTAKPRNGLAGDTETFRKVEPGQAIPLTAVPGQGPPPGGQPDPELLQFCRT
PNPALKNGQYWV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
Tag Myc-DDK
ACCN NM_001897
ORF Size 6966 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reference Data
RefSeq NM_001897.3, NM_001897.4, NP_001888
RefSeq Size 7896
RefSeq ORF 6969
Locus ID 1464
Cytogenetics 15q24.2
Protein Families Transmembrane
MW 250.4 kDa
Gene Summary A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq, Jul 2008].
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Citations (1)