SLC9A4 (NM_001011552) Human Untagged Clone

CAT#: SC301580

SLC9A4 (untagged)-Human solute carrier family 9 (sodium/hydrogen exchanger), member 4 (SLC9A4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC9A4
• Synonyms: NHE4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC301580 representing NM_001011552.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTCTGCAGATGTTCGTGACTTACAGTCCTTGGAATTGTTTGCTACTGCTAGTGGCTCTTGAGTGT
  TCTGAAGCATCTTCTGATTTGAATGAATCTGCAAATTCCACTGCTCAGTATGCATCTAACGCTTGGTTT
  GCTGCTGCCAGCTCAGAGCCAGAGGAAGGGATATCTGTTTTTGAACTGGATTATGACTATGTGCAAATT
  CCTTATGAGGTCACTCTCTGGATACTTCTAGCATCCCTTGCAAAAATAGGCTTCCACCTCTACCACAGG
  CTGCCAGGCCTCATGCCAGAAAGCTGCCTCCTCATCCTGGTGGGGGCGCTGGTGGGCGGCATCATCTTC
  GGCACCGACCACAAATCGCCTCCGGTCATGGACTCCAGCATCTACTTCCTGTATCTCCTGCCACCCATC
  GTTCTGGAGGGCGGCTACTTCATGCCCACCCGGCCCTTCTTTGAGAACATCGGCTCCATCCTGTGGTGG
  GCAGTATTGGGGGCCCTGATCAACGCCTTGGGCATTGGCCTCTCCCTCTACCTCATCTGCCAGGTGAAG
  GCCTTTGGCCTGGGCGACGTCAACCTGCTGCAGAACCTGCTGTTCGGCAGCCTGATCTCCGCCGTGGAC
  CCAGTGGCCGTGCTAGCCGTGTTTGAGGAAGCGCGCGTGAACGAGCAGCTCTACATGATGATCTTTGGG
  GAGGCCCTGCTCAATGATGGCATTACTGTGGTCTTATACAATATGTTAATTGCCTTTACAAAGATGCAT
  AAATTTGAAGACATAGAAACTGTCGACATTTTGGCTGGATGTGCCCGATTCATCGTTGTGGGGCTTGGA
  GGGGTATTGTTTGGCATCGTTTTTGGATTTATTTCTGCATTTATCACACGTTTCACTCAGAATATCTCT
  GCAATTGAGCCACTCATCGTCTTCATGTTCAGCTATTTGTCTTACTTAGCTGCTGAAACCCTCTATCTC
  TCCGGCATCCTGGCAATCACAGCCTGCGCAGTAACAATGAAAAAGTACGTGGAAGAAAACGTGTCCCAG
  ACATCATACACGACCATCAAGTACTTCATGAAGATGCTGAGCAGCGTCAGCGAGACCTTGATCTTCATC
  TTCATGGGTGTGTCCACTGTGGGCAAGAATCACGAGTGGAACTGGGCCTTCATCTGCTTCACCCTGGCC
  TTCTGCCAAATCTGGAGAGCCATCAGCGTATTTGCTCTCTTCTATATCAGTAACCAGTTTCGGACTTTC
  CCCTTCTCCATCAAGGACCAGTGCATCATTTTCTACAGTGGTGTTCGAGGAGCTGGAAGTTTTTCACTT
  GCATTTTTGCTTCCTCTGTCTCTTTTTCCTAGGAAGAAAATGTTTGTCACTGCTACTCTAGTAGTTATA
  TACTTTACTGTATTTATTCAGGGAATCACAGTTGGCCCTCTGGTCAGGTACCTGGATGTTAAAAAAACC
  AATAAAAAAGAATCCATCAATGAAGAGCTTCATATTCGTCTGATGGATCACTTAAAGGCTGGAATCGAA
  GATGTGTGTGGGCACTGGAGTCACTACCAAGTGAGAGACAAGTTTAAGAAGTTTGATCATAGATACTTA
  CGGAAAATCCTCATCAGAAAGAACCTACCCAAATCAAGCATTGTTTCTTTGTACAAGAAGCTGGAAATG
  AAGCAAGCCATCGAGATGGTGGAGACTGGGATACTGAGCTCTACAGCTTTCTCCATACCCCATCAGGCC
  CAGAGGATACAAGGAATCAAAAGACTTTCCCCTGAAGATGTGGAGTCCATAAGGGACATTCTGACATCC
  AACATGTACCAAGTTCGGCAAAGGACCCTGTCCTACAACAAATACAACCTCAAACCCCAAACAAGTGAG
  AAGCAGGCTAAAGAGATTCTGATCCGCCGCCAGAACACCTTAAGGGAGAGCATGAGGAAAGGTCACAGC
  CTGCCCTGGGGAAAGCCGGCTGGCACCAAGAATATCCGCTACCTCTCCTACCCCTACGGGAATCCTCAG
  TCTGCAGGAAGAGACACAAGGGCTGCTGGGTTCTCAGATGATGACAGCAGTGATCCAGGATCCCCATCC
  ATCACGTTCAGCGCATGCTCTCGGATAGGGTCACTTCAGAAGCAAGAGGCACAAGAAATAATACCAATG
  AAGAGCCTACACAGAGGAAGGAAGGCATTCAGCTTTGGTTATCAAAGAAACACAAGCCAAGAAGAGTAC
  TTGGGTGGAGTAAGGAGGGTGGCCTTAAGACCCAAACCTCTGTTTCATGCAGTGGATGAGGAGGGTGAG
  TCTGGAGGGGAGAGTGAGGGCAAGGCCTCTTTGGTTGAGGTTCGGTCGAGGTGGACAGCTGACCATGGA
  CACGGCAGGGACCATCACAGGTCCCATAGTCCTTTGCTCCAAAAAAAATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001011552
• Insert Size: 2397 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011552.3
• RefSeq Size: 4174 bp
• RefSeq ORF: 2397 bp
• Locus ID: 389015
• UniProt ID: Q6AI14
• Cytogenetics: 2q12.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 89.8 kDa
• Gene Summary: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. May play a specialized role in the kidney in rectifying cell volume in response to extreme fluctuations of hyperosmolar-stimulated cell shrinkage. Is relatively amiloride and ethylisopropylamiloride (EIPA) insensitive. Can be activated under conditions of hyperosmolar-induced cell shrinkage in a sustained intracellular acidification-dependence manner. Activated by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) in a sustained intracellular acidification-dependence manner. Affects potassium/proton exchange as well as sodium/proton and lithium/proton exchange. In basolateral cell membrane, participates in homeostatic control of intracellular pH, and may play a role in proton extrusion in order to achieve transepithelial HCO3(-) secretion. In apical cell membrane may be involved in mediating sodium absorption. Requires for normal levels of gastric acid secretion, secretory membrane development, parietal cell maturation and/or differentiation and at least secondarily for chief cell differentiation (By similarity).[UniProtKB/Swiss-Prot Function]