SLC9A4 (NM_001011552) Human Tagged ORF Clone

CAT#: RG212529

SLC9A4 (tGFP-tagged) - Human solute carrier family 9 (sodium/hydrogen exchanger), member 4 (SLC9A4)

Product Images

Circular map for RG212529

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLC9A4
• Synonyms: NHE4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG212529 representing NM_001011552
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCTGCAGATGTTCGTGACTTACAGTCCTTGGAATTGTTTGCTACTGCTAGTGGCTCTTGAGTGTT
  CTGAAGCATCTTCTGATTTGAATGAATCTGCAAATTCCACTGCTCAGTATGCATCTAACGCTTGGTTTGC
  TGCTGCCAGCTCAGAGCCAGAGGAAGGGATATCTGTTTTTGAACTGGATTATGACTATGTGCAAATTCCT
  TATGAGGTCACTCTCTGGATACTTCTAGCATCCCTTGCAAAAATAGGCTTCCACCTCTACCACAGGCTGC
  CAGGCCTCATGCCAGAAAGCTGCCTCCTCATCCTGGTGGGGGCGCTGGTGGGCGGCATCATCTTCGGCAC
  CGACCACAAATCGCCTCCGGTCATGGACTCCAGCATCTACTTCCTGTATCTCCTGCCACCCATCGTTCTG
  GAGGGCGGCTACTTCATGCCCACCCGGCCCTTCTTTGAGAACATCGGCTCCATCCTGTGGTGGGCAGTAT
  TGGGGGCCCTGATCAACGCCTTGGGCATTGGCCTCTCCCTCTACCTCATCTGCCAGGTGAAGGCCTTTGG
  CCTGGGCGACGTCAACCTGCTGCAGAACCTGCTGTTCGGCAGCCTGATCTCCGCCGTGGACCCAGTGGCC
  GTGCTAGCCGTGTTTGAGGAAGCGCGCGTGAACGAGCAGCTCTACATGATGATCTTTGGGGAGGCCCTGC
  TCAATGATGGCATTACTGTGGTCTTATACAATATGTTAATTGCCTTTACAAAGATGCATAAATTTGAAGA
  CATAGAAACTGTCGACATTTTGGCTGGATGTGCCCGATTCATCGTTGTGGGGCTTGGAGGGGTATTGTTT
  GGCATCGTTTTTGGATTTATTTCTGCATTTATCACACGTTTCACTCAGAATATCTCTGCAATTGAGCCAC
  TCATCGTCTTCATGTTCAGCTATTTGTCTTACTTAGCTGCTGAAACCCTCTATCTCTCCGGCATCCTGGC
  AATCACAGCCTGCGCAGTAACAATGAAAAAGTACGTGGAAGAAAACGTGTCCCAGACATCATACACGACC
  ATCAAGTACTTCATGAAGATGCTGAGCAGCGTCAGCGAGACCTTGATCTTCATCTTCATGGGTGTGTCCA
  CTGTGGGCAAGAATCACGAGTGGAACTGGGCCTTCATCTGCTTCACCCTGGCCTTCTGCCAAATCTGGAG
  AGCCATCAGCGTATTTGCTCTCTTCTATATCAGTAACCAGTTTCGGACTTTCCCCTTCTCCATCAAGGAC
  CAGTGCATCATTTTCTACAGTGGTGCTCGAGGAGCTGGAAGTTTTTCACTTGCATTTTTGCTTCCTCTGT
  CTCTTTTTCCTAGGAAGAAAATGTTTGTCACTGCCACTCTAGTAGTTATATACTTTACTGTATTTATTCA
  GGGAATCACAGTTGGCCCTCTGGTCAGGTACCTGGATGTTAAAAAAACCAATAAAAAAGAATCCATCAAT
  GAAGAGCTTCATATTCGTCTGATGGATCACTTAAAGGCTGGAATCGAAGATGTGTGTGGGCACTGGAGTC
  ACTACCAAGTGAGAGACAAGTTTAAGAAGTTTGATCATAGATACTTACGGAAAATCCTCATCAGAAAGAA
  CCTACCCAAATCAAGCATTGTTTCTTTGTACAAGAAGCTGGAAATGAAGCAAGCCATCGAGATGGTGGAG
  ACTGGGATACTGAGCTCTACAGCTTTCTCCATACCCCATCAGGCCCAGAGGATACAAGGAATCAAAAGAC
  TTTCCCCTGAAGATGTGGAGTCCATAAGGGACATTCTGACATCCAACATGTACCAAGTTCGGCAAAGGAC
  CCTGTCCTACAACAAATACAACCTCAAACCCCAAACAAGTGAGAAGCAGGCTAAAGAGATTCTGATCCGC
  CGCCAGAACACCTTAAGGGAGAGCATGAGGAAAGGTCACAGCCTGCCCTGGGGAAAGCCGGCTGGCACCA
  AGAATATCCGCTACCTCTCCTACCCCTACGGGAATCCTCAGTCTGCAGGAAGAGACACAAGGGCTGCTGG
  GTTCTCAGATGATGACAGCAGTGATCCAGGATCCCCATCCATCACGTTCAGCGCATGCTCTCGGATAGGG
  TCACTTCAGAAGCAAGAGGCACAAGAAATAATACCAATGAAGAGCCTACACAGAGGAAGGAAGGCATTCA
  GCTTTGGTTATCAAAGAAACACAAGCCAAGAAGAGTACTTGGGTGGAGTAAGGAGGGTGGCCTTAAGACC
  CAAACCTCTGTTTCATGCAGTGGATGAGGAGGGTGAGTCTGGAGGGGAGAGTGAGGGCAAGGCCTCTTTG
  GTTGAGGTTCGGTCGAGGTGGACAGCTGACCATGGACACAGCAGGGACCATCACAGGTCCCATAGTCCTT
  TGCTCCAAAAAAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG212529 representing NM_001011552
  Red=Cloning site Green=Tags(s)
  
  MALQMFVTYSPWNCLLLLVALECSEASSDLNESANSTAQYASNAWFAAASSEPEEGISVFELDYDYVQIP
  YEVTLWILLASLAKIGFHLYHRLPGLMPESCLLILVGALVGGIIFGTDHKSPPVMDSSIYFLYLLPPIVL
  EGGYFMPTRPFFENIGSILWWAVLGALINALGIGLSLYLICQVKAFGLGDVNLLQNLLFGSLISAVDPVA
  VLAVFEEARVNEQLYMMIFGEALLNDGITVVLYNMLIAFTKMHKFEDIETVDILAGCARFIVVGLGGVLF
  GIVFGFISAFITRFTQNISAIEPLIVFMFSYLSYLAAETLYLSGILAITACAVTMKKYVEENVSQTSYTT
  IKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFICFTLAFCQIWRAISVFALFYISNQFRTFPFSIKD
  QCIIFYSGARGAGSFSLAFLLPLSLFPRKKMFVTATLVVIYFTVFIQGITVGPLVRYLDVKKTNKKESIN
  EELHIRLMDHLKAGIEDVCGHWSHYQVRDKFKKFDHRYLRKILIRKNLPKSSIVSLYKKLEMKQAIEMVE
  TGILSSTAFSIPHQAQRIQGIKRLSPEDVESIRDILTSNMYQVRQRTLSYNKYNLKPQTSEKQAKEILIR
  RQNTLRESMRKGHSLPWGKPAGTKNIRYLSYPYGNPQSAGRDTRAAGFSDDDSSDPGSPSITFSACSRIG
  SLQKQEAQEIIPMKSLHRGRKAFSFGYQRNTSQEEYLGGVRRVALRPKPLFHAVDEEGESGGESEGKASL
  VEVRSRWTADHGHSRDHHRSHSPLLQKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001011552
• ORF Size: 2394 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001011552.2
• RefSeq Size: 4165 bp
• RefSeq ORF: 2397 bp
• Locus ID: 389015
• UniProt ID: Q6AI14
• Cytogenetics: 2q12.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. May play a specialized role in the kidney in rectifying cell volume in response to extreme fluctuations of hyperosmolar-stimulated cell shrinkage. Is relatively amiloride and ethylisopropylamiloride (EIPA) insensitive. Can be activated under conditions of hyperosmolar-induced cell shrinkage in a sustained intracellular acidification-dependence manner. Activated by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS) in a sustained intracellular acidification-dependence manner. Affects potassium/proton exchange as well as sodium/proton and lithium/proton exchange. In basolateral cell membrane, participates in homeostatic control of intracellular pH, and may play a role in proton extrusion in order to achieve transepithelial HCO3(-) secretion. In apical cell membrane may be involved in mediating sodium absorption. Requires for normal levels of gastric acid secretion, secretory membrane development, parietal cell maturation and/or differentiation and at least secondarily for chief cell differentiation (By similarity).[UniProtKB/Swiss-Prot Function]