IKB zeta (NFKBIZ) (NM_001005474) Human Untagged Clone

CAT#: SC300963

NFKBIZ (untagged)-Human nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, zeta (NFKBIZ), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IKB zeta
• Synonyms: IKBZ; INAP; MAIL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001005474 edited
  AGAGGCGGCGCCCGCGCCGAGCGCCAGCCAGTTGAGCCCCATATGGGGGTTGGCAGGCAG
  CAGAGAGGCCCCTTTCAAGGTGTTCGGGTAAAGAACTCAGTGAAGGAACTCCTGTTGCAC
  ATCCGAAGTCATAAACAGAAGGCTTCTGGCCAAGCTGTGGATGATTTTAAGACACAAGGT
  GTGAACATAGAACAGTTCAGAGAATTGAAGAACACAGTATCATACAGTGGGAAAAGGAAA
  GGGCCCGATTCGTTGTCTGATGGACCTGCTTGCAAAAGGCCAGCTCTGTTGCATTCCCAA
  TTTTTGACACCACCTCAAACACCAACGCCCGGGGAGAGCATGGAAGATGTTCATCTCAAT
  GAACCCAAACAGGAGAGCAGTGCTGATCTGCTTCAGAACATTATCAACATTAAGAATGAA
  TGCAGCCCCGTTTCCCTGAACACAGTTCAAGTTAGCTGGCTGAACCCCGTGGTGGTCCCT
  CAGAGCTCCCCCGCAGAGCAGTGTCAGGACTTCCATGGAGGGCAGGTCTTTTCTCCACCT
  CAGAAATGCCAACCATTCCAAGTCAGGGGCTCCCAACAAATGATAGACCAGGCTTCCCTG
  TACCAGTATTCTCCACAGAACCAGCATGTAGAGCAGCAGCCACACTACACCCACAAACCA
  ACTCTGGAATACAGTCCTTTTCCCATACCTCCCCAGTCCCCCGCTTATGAACCAAACCTC
  TTTGATGGTCCAGAATCACAGTTTTGCCCAAACCAAAGCTTAGTTTCCCTTCTTGGTGAT
  CAAAGGGAATCTGAGAATATTGCTAATCCCATGCAGACTTCCTCCAGTGTTCAGCAGCAA
  AATGATGCTCACTTGCACAGCTTCAGCATGATGCCCAGCAGCGCCTGTGAGGCCATGGTG
  GGGCACGAGATGGCCTCTGACTCTTCAAACACTTCACTGCCATTCTCAAACATGGGAAAT
  CCAATGAACACCACACAGTTAGGGAAATCACTTTTTCAGTGGCAGGTGGAGCAGGAAGAA
  AGCAAATTGGCAAATATTTCCCAAGACCAGTTTCTTTCAAAGGATGCAGATGGTGACACG
  TTCCTTCATATTGCTGTTGCCCAAGGGAGAAGGGCACTTTCCTATGTTCTTGCAAGAAAG
  ATGAATGCACTTCACATGCTGGATATTAAAGAGCACAATGGACAGAGTGCCTTTCAGGTG
  GCAGTGGCTGCCAATCAGCATCTCATTGTGCAGGATCTGGTGAACATCGGGGCACAGGTG
  AACACCACAGACTGCTGGGGAAGAACACCTCTGCATGTGTGTGCTGAGAAGGGCCACTCC
  CAGGTGCTTCAGGCGATTCAGAAGGGAGCAGTGGGAAGTAATCAGTTTGTGGATCTTGAG
  GCAACTAACTATGATGGCCTGACTCCCCTTCACTGTGCAGTCATAGCCCACAATGCTGTG
  GTCCATGAACTCCAGAGAAATCAACAGCCTCATTCACCTGAAGTTCAGGAGCTTTTACTG
  AAGAATAAGAGTCTGGTTGATACCATTAAGTGCCTAATTCAAATGGGAGCAGCGGTGGAA
  GCGAAGGATCGCAAAAGTGGCCGCACAGCCCTGCATTTGGCAGCTGAAGAAGCAAATCTG
  GAACTCATTCGCCTCTTTTTGGAGCTGCCCAGTTGCCTGTCTTTTGTGAATGCAAAGGCT
  TACAGTGGCAACACTGCCCTCCATGTTGCTGCCAGCTTGCAGTATCGGTTGACACAATTA
  GATGCTGTCCGCCTGTTGATGAGGAAGGGAGCAGACCCAAGTACTCGGAACTTGGAGAAC
  GAACAGCCAGTGCATTTGGTTCCCGATGGCCCTGTGGGAGAACAGATCCGACGTATCCTG
  AAGGGAAAGTCCATTCAGCAGAGAGCTCCACCGTATTAGCTCCATTAGCTTGGAGCCTGG
  CTAGCAACACTCACTGTCAGTTAGGCAGTCCTGATGTATCTGTACATAGACCATTTGCCT
  TATATTGGCAAATGTAAGTTGTTTCTATGAAACAAACATATTTAGTTCACTATTATATAG
  TGGGTTATATTAAAAGAAAAGAAGAAAAATATCTAATTTCTCTTGGCAGATTTGCATATT
  TCATACCCAGGTATCTGGGATCTAGACATCTGAATTTGATCTCAATGGTAACATTGCCTT
  CAATTAACAGTAGCTTTTGAGTAGGAAAGGACTTTGATTTGTGGCACAAAACATTATTAA
  TATAGCTATTGACAGTTTCAAAGCAGGTAAATTGTAAATGTTTCTTTAAGAAAAAGCATG
  TGAAAGGAAAAAGGTAAATACAGCATTGAGGCTTCATTTGGCCTTAGTCCCTGGGAGTTA
  CTGGCGTTGGACAGGCTTCAGTCATTGGACTAGATGAAAGGTGTCCATGGTTAGAATTTG
  ATCTTTGCAAACTGTATATAATTGTTATTTTTGTCCTTAAAAATATTGTACATACTTGGT
  TGTTAACATGGTCATATTTGAAATGTATAAGTCCATAAAATAGAAAAGAACAAGTGAATT
  GTTGCTATTTAAAAAAATTTTACAATTCTTACTAAGGAGTTTTTATTGTGTAATCACTAA
  GTCTTTGTAGATAAAGCAGATGGGGAGTTACGGAGTTGTTCCTTTACTGGCTGAAAGATA
  TATTCGAATTGTAAAGATGCTTTTTCTCATGCATTGAAATTATACATTATTTGTAGGGAA
  TTGCATGCTTTTTTTTTTTTTTCTCCCGAGACAGGGTCTTGCTCTGGCGCCCAGGCTGGA
  GTACAGTGGCATGATCTTGGCTCACTTCAGCCTTGACTTGGGCTCAAGTGATCCTCCTAC
  CTGAGCCTTCTGAGTAACTGGAACTACAGGTGTGCACTCCTCGCCTGGCTAATTTTTTAT
  TTTTTGTACAGGCAGGATCTTGCCACCTTGCCCAGGCTGGTCTTGAACTCCTGAGCTCAT
  GCCATCTGCCTGCCTTAGTCTCCCAAAATGCTGGGATTACAGGAGTGAGCCACCATGCCC
  GGCTGGCAGTTGCATGGAAGAGAACACCTCTTTATGGCTTACCCTCTAGAATTTCTAATT
  TATGTGTTCTGTTGAAATTTTTGTTTTTTTACCTTTATTGAAACAACAAAAAGTCAGTAT
  TGAAACATATCTTCCTGTTTTCTGTTGTCAAATGATGATAATGTGCCATGATGTTTTATA
  TATATCATTCAGAAAAAGTTTTATTTTTTAATAACATTCTATTAACATTATTTTGCTTGC
  CGCTGGCATGCCTGAGGAATGTATTTGGCTTTGATTACACACTAAGTTTTTGTAATAAAT
  TTGACTCATTAAAAACCTTTTTTTTTAAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001005474
• Insert Size: 3300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_001005474.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005474.1, NP_001005474.1
• RefSeq Size: 3782 bp
• RefSeq ORF: 1857 bp
• Locus ID: 64332
• UniProt ID: Q9BYH8
• Cytogenetics: 3q12.3
• Protein Families: Druggable Genome
• Gene Summary: This gene is a member of the ankyrin-repeat family and is induced by lipopolysaccharide (LPS). The C-terminal portion of the encoded product which contains the ankyrin repeats, shares high sequence similarity with the I kappa B family of proteins. The latter are known to play a role in inflammatory responses to LPS by their interaction with NF-B proteins through ankyrin-repeat domains. Studies in mouse indicate that this gene product is one of the nuclear I kappa B proteins and an activator of IL-6 production. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) contains alternative 5' non-coding exons compared to variant 1, and initiates translation from an in-frame downstream AUG, resulting in a shorter isoform (b) compared to isoform a.