C10orf90 (NM_001004298) Human Untagged Clone

CAT#: SC300618

C10orf90 (untagged)-Human chromosome 10 open reading frame 90 (C10orf90)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C10orf90
• Synonyms: bA422P15.2; FATS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001004298 edited
  ATGCTCAAACTGTCTGGAGAAGGATTACGGGACAGCTACCACAGTAGAAGAGATCAAATT
  GCCCTTAAAAATCTCCAGTCTGATGTCACAGAGGCAAAATCTGACTTCACCAAGGAGACC
  CTGGCATCACAAAACACAAAAATGATTTCATCCATAGTCATCTCCCAGATGATTGATGAG
  AATAAGTCAAGAGAAAACAGGGCCTCCTTGCCCCTACCGTGTGCCATTGCTCAGTCTCGT
  GCACATCACGCCAAGCAATCTCTGGCTAACCGCAGCGGAGTCAACATTCACAGAGCATTT
  GCGTTACTTCCGGGCAGATTAGGAATCCCGGCACCGTCAGATGAGCGAGGACCTGAGGCA
  GAGCTGCCGCCCAAAGAGGAGAGACCCTGTGGGGGCCCCCGCAGAGGGTTTGCATCCATC
  ACCATCACGGCCAGACGCGTGGGCCCCCCAGCCCGCGCCCTGGTGTGGGGGACTGCTGGG
  GACTCTCTGTGCCCCAAGTGCAGGGCTGAGGACACACTGTTCCAGGCGCCCCCGGCTCTG
  GCCAATGGCGCCCATCCAGGTCGGCATCAGAGATCTTTTGCCTGCACAGAGTTCTCCAGA
  AACAGCTCCGTGGTGCGGCTGAAGGTTCCCGAGGCCCACACTGGGTTGTGTGAGAGACGC
  AAGTACTGGGTCACCCATGCAGACGACAAAGAGACCAGTTTTTCTCCAGACACCCCACTG
  TCAGGAAAGAGCCCGCTGGTGTTCAGTTCCTGTGTCCACCTCAGGGTGTCTCAGCAGTGT
  CCAGATTCAATCTATTACGTAGACAAGTCTCTCTCCGTCCCCATTGAGCCACCTCAAATT
  GCCAGCCCCAAAATGCACAGATCCGTCCTGTCGCTCAACCTCAATTGTAGTTCCCACAGA
  TTAACAGCAGATGGAGTAGATGGCCTAGTGAACAGAGAGCCAATAAGCGAAGCCCTGAAG
  CAGGAGCTCCTGGAGGGAGACCAGGACCTCGTAGGCCAGCGCTGGAACCCAGGTTTACAA
  GAAAGTCACTTGAAGGAAACCCCATCGTTGAGGCGGGTGCATTTGGGGACCGGCGCTTGT
  CCTTGGAGTGGTTCTTTTCCATTGGAAAACACAGAATTGGCAAATGTGGGAGCTAACCAA
  GTCACTGTAAGAAAAGGGGAAAAGGACCATACAACTCATTGTCACGCCAGCGATCATGCC
  AACCAACTGTCCATTCACATTCCTGGCTGGAGTTACAGGGCAGTACACACAAAAGTATTT
  TCTGGAAGCAGCAAGAGGCAACAAGGAGAAGTATGTATGACTGTGTCTGCTCCTCCAGTG
  GAACAGAAGCCCACTAGACATTTCCTTCCCATTGGGGATAGCTCTCCAAGCGATGACTGT
  CTGTCTAGAGACCTTTCTGAGCCCACAGAGAGACGACATCAAAGCTTCCTGAAACCCAGA
  ATCCTTTTTCCTGGGTTTCTTTGCCCCTTACAAGATGTATGTGCATCTCTGCAGGAAGAC
  AATGGTGTTCAGATAGAAAGCAAGTTCCCCAAAGGAGATTACACATGCTGTGACTTGGTT
  GTAAAAATAAAGGAATGTAAGAAGAGTGAGGACCCCACCACACCTGAGCCCTCCCCAGCA
  GCACCCTCGCCAGCACCCCGCGATGGAGCAGGGAGCCCTGGCCTGTCCGAAGACTGTTCT
  GAGTCTCAGCAAACGCCTGCAAGATCTTTGACCTTGCAAGAAGCACTGGAAGTTCGTAAG
  CCTCAGTTCATTTCTCGCTCACAAGAACGGCTGAAGAAGCTTGAACACATGGTCCAGCAG
  AGGAAAGCCCAGCGGAAGGAGGACCTGAGGCAGAAGCAGAGCCTCCTTCCCATCCGCACC
  AGCAAGAAGCAGTTCACGATTCCCCATCCTCTGAGTGATAACTTGTTCAAACCCAAAGAA
  CGGTGCATTTCAGAGAAGGAGATGCATATGCGATCTAAGAGAATCTATGATAACTTGCCG
  GAAGTTAAAAAGAAAAAAGAAGAACAAAGGAAAAGGGTGATCTTACAAAGTAACAGACTC
  CGTGCCGAAGTCTTCAAAAAGCAATTACTGGACCAGCTCCTTCAAAGGAATGCGGTCTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001004298
• Insert Size: 2100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004298.2, NP_001004298.2
• RefSeq Size: 3097 bp
• RefSeq ORF: 2100 bp
• Locus ID: 118611
• UniProt ID: Q96M02
• Cytogenetics: 10q26.2
• Gene Summary: Tumor suppressor that is required to sustain G2/M checkpoint after DNA damage. Acts as a p53/TP53 activator by inhibiting MDM2 binding to p53/TP53 and stimulating non-proteolytic polyubiquitination of p53/TP53. Exhibits ubiquitin ligase (E3) activity and assemble ubiquitin polymers through 'Lys-11'- (K11-), 'Lys-29'- (K29-) and 'Lys-63'- (K63)-linkages, independently of the ubiquitin-conjugating enzyme (E2). Promotes p53/TP53-dependent transcription of CDKN1A/p21, leading to robust checkpoint response. Mediates CDKN1A/p21 protein stability in a ubiquitin-independent manner. Interacts with HDAC1 and prevents binding of HDAC1 to CDKN1A/p21 and facilitates the acetylation and stabilization of CDKN1A/p21 (By similarity). May have a role in the assembly of primary cilia (Probable).[UniProtKB/Swiss-Prot Function]