TOP3B (NM_003935) Human Untagged Clone

CAT#: SC127952

TOP3B (untagged)-Human topoisomerase (DNA) III beta (TOP3B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TOP3B
• Synonyms: TOP3B1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003935, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGACTGTGCTCATGGTTGCTGAAAAGCCGTCCTTGGCACAGTCAATTGCCAAAATCCTCTCTAGAG
  GGAGCCTGTCCTCACACAAAGGGCTGAACGGGGCCTGCTCAGTCCACGAGTACACTGGGACCTTTGCTGG
  CCAGCCAGTGCGCTTCAAGATGACGTCTGTCTGTGGTCACGTGATGACCCTGGATTTCCTGGGAAAATAC
  AACAAATGGGACAAAGTGGACCCCGCAGAACTGTTCAGCCAAGCTCCCACGGAGAAGAAAGAAGCTAACC
  CCAAGCTGAACATGGTGAAGTTCCTGCAGGTGGAGGGCAGAGGCTGCGACTACATCGTGCTGTGGCTGGA
  CTGCGACAAGGAGGGGGAGAACATCTGCTTTGAGGTTCTTGATGCTGTTCTGCCCGTCATGAACAAGGCC
  CATGGTGGCGAGAAGACCGTGTTCCGGGCCAGGTTTAGCTCCATCACGGACACAGACATCTGTAATGCCA
  TGGCCTGCCTAGGCGAGCCTGACCACAACGAGGCGCTCTCAGTGGATGCTCGCCAGGAGCTGGACCTGCG
  AATCGGCTGTGCATTCACCAGGTTTCAGACTAAATATTTCCAGGGGAAATACGGTGATTTAGACAGCTCT
  CTCATCTCCTTTGGGCCGTGTCAGACTCCAACCCTGGGATTCTGTGTGGAGAGACATGATAAAATCCAGT
  CCTTCAAACCAGAGACCTACTGGGTGCTGCAGGCCAAGGTTAACACTGACAAAGACAGATCTCTCCTTTT
  GGACTGGGACCGAGTAAGAGTGTTTGACCGGGAGATCGCACAGATGTTTTTAAACATGACAAAGCTGGAG
  AAGGAAGCCCAGGTGGAGGCCACAAGCAGGAAAGAAAAGGCCAAGCAGAGGCCCCTGGCCCTGAACACTG
  TGGAGATGCTGCGTGTGGCCAGCTCTTCTCTGGGCATGGGGCCGCAGCACGCCATGCAGACGGCTGAGCG
  GCTCTACACGCAAGGCTACATCAGCTACCCACGGACAGAGACCACCCACTACCCTGAGAACTTTGACCTG
  AAGGGCTCTCTGCGGCAGCAGGCCAACCACCCCTACTGGGCCGACACGGTGAAGCGGTTGTTAGCAGAAG
  GTATCAACCGCCCGCGGAAAGGCCATGACGCCGGCGACCATCCCCCCATCACCCCCATGAAGTCTGCCAC
  AGAGGCCGAATTAGGGGGTGACGCGTGGCGGCTCTATGAGTACATCACCAGACACTTCATCGCCACGGTC
  AGCCATGACTGCAAGTACCTGCAGAGCACCATCTCCTTCAGAATTGGGCCCGAGCTCTTCACCTGCTCCG
  GGAAGACCGTCCTCTCACCAGGCTTCACGGAGGTCATGCCCTGGCAGAGCGTGCCCCTGGAGGAGAGCCT
  GCCCACTTGCCAGCGGGGTGATGCCTTCCCTGTGGGCGAGGTGAAGATGCTGGAGAAGCAGACGAACCCA
  CCCGACTACCTGACGGAGGCCGAGCTCATCACGCTCATGGAGAAGCATGGCATCGGCACGGATGCCAGCA
  TCCCTGTGCATATCAACAACATCTGCCAGCGCAACTATGTCACGGTGGAGAGCGGGCGCCGGCTCAAGCC
  CACCAACCTCGGCATCGTCCTGGTGCACGGCTACTATAAGATTGATGCAGAGCTGGTGCTCCCCACCATC
  CGCAGTGCAGTGGAGAAGCAGCTGAACCTGATCGCCCAGGGCAAGGCCGACTACCGCCAGGTCCTGGGCC
  ACACCCTGGACGTGTTCAAGAGGAAGTTCCACTACTTTGTCGACTCCATTGCTGGCATGGATGAGTTGAT
  GGAGGTGTCTTTCTCGCCCCTGGCGGCCACAGGCAAGCCCCTCTCACGCTGTGGGAAGTGCCACCGCTTC
  ATGAAGTACATCCAGGCCAAGCCAAGCCGCCTGCACTGCTCCCACTGCGATGAGACCTACACGCTCCCCC
  AGAACGGCACCATCAAGCTCTACAAGGAGCTCCGCTGCCCTCTGGATGACTTCGAGCTGGTCCTGTGGTC
  ATCAGGCTCTCGGGGCAAGAGCTACCCGCTGTGCCCCTACTGCTACAACCACCCACCCTTCCGAGACATG
  AAGAAAGGCATGGGCTGCAACGAGTGTACGCACCCCTCCTGCCAGCACTCGCTGAGCATGCTGGGCATCG
  GCCAGTGCGTGGAATGTGAGAGCGGGGTGCTGGTGCTGGACCCCACCTCGGGCCCCAAGTGGAAGGTGGC
  CTGCAACAAGTGCAACGTGGTAGCGCACTGCTTCGAGAACGCCCACCGCGTGCGGGTGTCCGCCGACACC
  TGCAGTGTCTGTGAGGCCGCCTTGCTTGATGTGGACTTCAACAAGGCCAAGTCCCCACTCCCGGGCGATG
  AGACGCAGCACATGGGCTGCGTCTTTTGTGACCCCGTCTTCCAGGAGCTGGTGGAGCTGAAGCATGCGGC
  CTCCTGCCACCCCATGCACCGCGGTGGACCAGGGAGAAGGCAGGGTCGAGGGCGGGGCCGGGCCAGGAGG
  CCCCCTGGGAAGCCCAACCCCAGACGGCCCAAGGACAAGATGTCAGCCCTGGCCGCCTACTTTGTATGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003935 unedited
  GAGTCTAAACTTTTTCATTGCCAGTGCCCCGAAACTGGAGTGTGAAGGACCGAGACAAAA
  TGAAGACTGTGCTCATGGTTGCTGAAAAGCCGTCCTTGGCACAGTCAATTGCCAAAATCC
  TCTCTAGAGGGAGCCTGTCCTCACACAAAGGGCTGAACGGGGCCTGCTCAGTCCACGAGT
  ACACTGGGACCTTTGCTGGCCAGCCAGTGCGCTTCAAGATGACGTCTGTCTGTGGTCACG
  TGATGACCCTGGATTTCCTGGGAAAATACAACAAATGGGACAAAGTGGACCCCGCAGAAC
  TGTTCAGCCAAGCTCCCACGGAGAAGAAAGAAGCTAACCCCAAGCTGAACATGGTGAAGT
  TCCTGCAGGTGGAGGGCAGAGGCTGCGACTACATCGTGCTGTGGCTGGACTGCGACAAGG
  AGGGGGAGAACATCTGCTTTGAGGTTCTTGATGCTGTTCTGCCCGTCATGAACAAGGCCC
  ATGGTGGCGAGAAGACCGTGTTCCGGGCCAGGTTTAGCTCCATCACGGACACAGACATCT
  GTAATGCCCTGGCCTGCCTAGGCGAGCCTGACCACAACGAGGCGCTCTCAGTGGATGCTC
  GCCAGGAGCTGGACCTGCGAATCGGCTGGGCATTCCCAAGTTTCAGACTAAATTTTTCAG
  GGGAAAATCGTGATTTAAACGGCTCTTCATTTCCTTTGGGCGTGTCAACTTCACCCTGGG
  ATCTGGGGGGAGAACATGAAAAATCCAGTCCTTAAACCAAAACCTATGGGTCTGCAGGCC
  AGGTAAACTGGCAAGACGATTCTTCTTGGACGGGCCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_003935 unedited
  ACCGCGGCCGCATCTAAATTTTTTTTTTTTTTTTTCAAAAAAAATGTTTTAATATATGAT
  ATTAATATATTCACCTTTATACCAATGCCGTTGGAAAGTATAATTTTTTAAATGGAGGAT
  GGGGCACCAGATCTTATGTGGCTCTGCCCACCTGACCTTCAGGTCCGCAGGCCTGTGTCC
  TTGCCAGGAATGAATGTCTCTGGGATGCTTCTCTAACAGGAGGAACCTGGAGTGAGACCA
  GGAACCTGGAGAACTAGCAGCTGGGTGACTGGAAAGGAAACCATCTGGGGTGGGGTTGGG
  TCTGCAAGGCAGGAGGTGTCTGGGCAGAGAAAGCAGGGCTGGGGCGGAGGGTCCTGCCTA
  ATCCCATTCTCCTGCTCACTCTTTCCTAGACCCCTGTCAGGGCATTATCCATGATCAAGG
  CCTGAGGCTACTTTTTATAAACAGCGTTCCTGGGCCCCTCCCTTAGCCTACCCTGACCCT
  ATTTCCTTGACGAACATACCCTTCTATTTACCTCCCCCTCCATCTCAGCGGTTCGCTCCC
  ATACCCCGCCTCCCGTCTCTCATCCCCTCCTGCTTTCTCTTCTACCCACTGTCTTCGCTT
  CTTCTGTCTACCCCTCAACCTGCCCGGTCTCTCGCGCCTAGCCTTTCCCTTCTCCATCTC
  CTTCCTCCTCCGTCCTTCTTCTCTATTCTTCCCTTTCCATCCCCCCACTTTTCTCCTTTC
  CTACCATCTCCCTTCTTCCCCCCTTCTATTCCCCTCTTTTTTTTTTCTCCCTTCCCCTCC
  CTTTTCTTTTTCTCCTTTCTCCCCCCCCCCACTTTCCCCCTCTCCTTTTCCCTTCCTTTA
  TTCCCCTCTCTCCCCGCCTTTTTCCCCCCCCATCCTCCTTTTTATTCACCTCCCCTCACC
  CCCCCTCCCGCTCCTGTTCCAGTCTACACTCACCCCCATTCTTCCCCTCTCTGTCTTTCC
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003935
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003935.3, NP_003926.1
• RefSeq Size: 3124 bp
• RefSeq ORF: 2589 bp
• Locus ID: 8940
• UniProt ID: O95985
• Cytogenetics: 22q11.22
• Domains: Topoisom_bac, TOP1Bc, TOP1Ac, TOPRIM, Toprim
• Protein Pathways: Homologous recombination
• Gene Summary: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1-5 encode the same protein (isoform 1).