TOP3B (NM_003935) Human Tagged ORF Clone

CAT#: RG223204

TOP3B (tGFP-tagged) - Human topoisomerase (DNA) III beta (TOP3B)

Product Images

Circular map for RG223204

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TOP3B
• Synonyms: TOP3B1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG223204 representing NM_003935
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGACTGTGCTCATGGTTGCTGAAAAGCCGTCCTTGGCACAGTCAATTGCCAAAATCCTCTCTAGAG
  GGAGCCTGTCCTCACACAAAGGGCTGAACGGGGCCTGCTCAGTCCACGAGTACACTGGGACCTTTGCTGG
  CCAGCCAGTGCGCTTCAAGATGACGTCTGTCTGTGGTCACGTGATGACCCTGGATTTCCTGGGAAAATAC
  AACAAATGGGACAAAGTGGACCCCGCAGAACTGTTCAGCCAAGCTCCCACGGAGAAGAAAGAAGCTAACC
  CCAAGCTGAACATGGTGAAGTTCCTGCAGGTGGAGGGCAGAGGCTGCGACTACATCGTGCTGTGGCTGGA
  CTGCGACAAGGAGGGGGAGAACATCTGCTTTGAGGTTCTTGATGCTGTTCTGCCCGTCATGAACAAGGCC
  CATGGTGGCGAGAAGACCGTGTTCCGGGCCAGGTTTAGCTCCATCACGGACACAGACATCTGTAATGCCA
  TGGCCTGCCTAGGCGAGCCTGACCACAACGAGGCGCTCTCAGTGGATGCTCGCCAGGAGCTGGACCTGCG
  AATCGGCTGTGCATTCACCAGGTTTCAGACTAAATATTTCCAGGGGAAATACGGTGATTTAGACAGCTCT
  CTCATCTCCTTTGGGCCGTGTCAGACTCCAACCCTGGGATTCTGTGTGGAGAGACATGATAAAATCCAGT
  CCTTCAAACCAGAGACCTACTGGGTGCTGCAGGCCAAGGTTAACACTGACAAAGACAGATCTCTCCTTTT
  GGACTGGGACCGAGTAAGAGTGTTTGACCGGGAGATCGCACAGATGTTTTTAAACATGACAAAGCTGGAG
  AAGGAAGCCCAGGTGGAGGCCACAAGCAGGAAAGAAAAGGCCAAGCAGAGGCCCCTGGCCCTGAACACTG
  TGGAGATGCTGCGTGTGGCCAGCTCTTCTCTGGGCATGGGGCCGCAGCACGCCATGCAGACGGCTGAGCG
  GCTCTACACGCAAGGCTACATCAGCTACCCACGGACAGAGACCACCCACTACCCTGAGAACTTTGACCTG
  AAGGGCTCTCTGCGGCAGCAGGCCAACCACCCCTACTGGGCCGACACGGTGAAGCGGTTGTTAGCAGAAG
  GTATCAACCGCCCGCGGAAAGGCCATGACGCCGGCGACCATCCCCCCATCACCCCCATGAAGTCTGCCAC
  AGAGGCCGAATTAGGGGGTGACGCGTGGCGGCTCTATGAGTACATCACCAGACACTTCATCGCCACGGTC
  AGCCATGACTGCAAGTACCTGCAGAGCACCATCTCCTTCAGAATTGGGCCCGAGCTCTTCACCTGCTCCG
  GGAAGACCGTCCTCTCACCAGGCTTCACGGAGGTCATGCCCTGGCAGAGCGTGCCCCTGGAGGAGAGCCT
  GCCCACTTGCCAGCGGGGTGATGCCTTCCCTGTGGGCGAGGTGAAGATGCTGGAGAAGCAGACGAACCCA
  CCCGACTACCTGACGGAGGCCGAGCTCATCACGCTCATGGAGAAGCATGGCATCGGCACGGATGCCAGCA
  TCCCTGTGCATATCAACAACATCTGCCAGCGCAACTATGTCACGGTGGAGAGCGGGCGCCGGCTCAAGCC
  CACCAACCTCGGCATCGTCCTGGTGCACGGCTACTATAAGATTGATGCAGAGCTGGTGCTCCCCACCATC
  CGCAGTGCAGTGGAGAAGCAGCTGAACCTGATCGCCCAGGGCAAGGCCGACTACCGCCAGGTCCTGGGCC
  ACACCCTGGACGTGTTCAAGAGGAAGTTCCACTACTTTGTCGACTCCATTGCTGGCATGGATGAGTTGAT
  GGAGGTGTCTTTCTCGCCCCTGGCGGCCACAGGCAAGCCCCTCTCACGCTGTGGGAAGTGCCACCGCTTC
  ATGAAGTACATCCAGGCCAAGCCAAGCCGCCTGCACTGCTCCCACTGCGATGAGACCTACACGCTCCCCC
  AGAACGGCACCATCAAGCTCTACAAGGAGCTCCGCTGCCCTCTGGATGACTTCGAGCTGGTCCTGTGGTC
  ATCAGGCTCTCGGGGCAAGAGCTACCCGCTGTGCCCCTACTGCTACAACCACCCACCCTTCCGAGACATG
  AAGAAAGGCATGGGCTGCAACGAGTGTACGCACCCCTCCTGCCAGCACTCGCTGAGCATGCTGGGCATCG
  GCCAGTGCGTGGAATGTGAGAGCGGGGTGCTGGTGCTGGACCCCACCTCGGGCCCCAAGTGGAAGGTGGC
  CTGCAACAAGTGCAACGTGGTAGCGCACTGCTTCGAGAACGCCCACCGCGTGCGGGTGTCCGCCGACACC
  TGCAGTGTCTGTGAGGCCGCCTTGCTTGATGTGGACTTCAACAAGGCCAAGTCCCCACTCCCGGGCGATG
  AGACGCAGCACATGGGCTGCGTCTTTTGTGACCCCGTCTTCCAGGAGCTGGTGGAGCTGAAGCATGCGGC
  CTCCTGCCACCCCATGCACCGCGGTGGACCAGGGAGAAGGCAGGGTCGAGGGCGGGGCCGGGCCAGGAGG
  CCCCCTGGGAAGCCCAACCCCAGACGGCCCAAGGACAAGATGTCAGCCCTGGCCGCCTACTTTGTA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG223204 representing NM_003935
  Red=Cloning site Green=Tags(s)
  
  MKTVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVMTLDFLGKY
  NKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEGENICFEVLDAVLPVMNKA
  HGGEKTVFRARFSSITDTDICNAMACLGEPDHNEALSVDARQELDLRIGCAFTRFQTKYFQGKYGDLDSS
  LISFGPCQTPTLGFCVERHDKIQSFKPETYWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLE
  KEAQVEATSRKEKAKQRPLALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDL
  KGSLRQQANHPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATV
  SHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVGEVKMLEKQTNP
  PDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGIVLVHGYYKIDAELVLPTI
  RSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDSIAGMDELMEVSFSPLAATGKPLSRCGKCHRF
  MKYIQAKPSRLHCSHCDETYTLPQNGTIKLYKELRCPLDDFELVLWSSGSRGKSYPLCPYCYNHPPFRDM
  KKGMGCNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFENAHRVRVSADT
  CSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCHPMHRGGPGRRQGRGRGRARR
  PPGKPNPRRPKDKMSALAAYFV
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003935
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003935.5
• RefSeq Size: 3124 bp
• RefSeq ORF: 2589 bp
• Locus ID: 8940
• UniProt ID: O95985
• Cytogenetics: 22q11.22
• Domains: Topoisom_bac, TOP1Bc, TOP1Ac, TOPRIM, Toprim
• Protein Pathways: Homologous recombination
• Gene Summary: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]