RDH12 (NM_152443) Human Untagged Clone

CAT#: SC126349

RDH12 (untagged)-Human retinol dehydrogenase 12 (all-trans/9-cis/11-cis) (RDH12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RDH12
• Synonyms: LCA13; RP53; SDR7C2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_152443 edited
  GGATTCTGGGCTCTGCTCAGAGTCAGGCTGCTGCTCAGCACCCAGGACGGAGAGGAGCAG
  AGAAGCAGCAGAAGCAGCCAAGAGCTGGAGCCAGACCAGGAACCTGAGCCAGAGCTGGGG
  TTGAAGCTGGAGCAGCAGCAAAAGCAACAGCAGCTACAGAAGTTGGAACGATGCTGGTCA
  CCTTGGGACTGCTCACCTCCTTCTTCTCGTTCCTGTATATGGTAGCTCCATCCATCAGGA
  AGTTCTTTGCTGGTGGAGTGTGTAGAACAAATGTGCAGCTTCCTGGCAAGGTAGTGGTGA
  TCACTGGCGCCAACACGGGCATTGGCAAGGAGACGGCCAGAGAGCTCGCTAGCCGAGGAG
  CCCGAGTCTATATTGCCTGCAGAGATGTACTGAAGGGGGAGTCTGCTGCCAGTGAAATCC
  GAGTGGATACAAAGAACTCCCAGGTGCTGGTGCGGAAATTGGACCTATCCGACACCAAAT
  CTATCCGAGCCTTTGCTGAGGGCTTTCTGGCAGAGGAAAAGCAGCTCCATATTCTGATCA
  ACAATGCGGGAGTAATGATGTGTCCATATTCCAAGACAGCTGATGGCTTTGAAACCCACC
  TGGGAGTCAACCACCTGGGCCACTTCCTCCTCACCTACCTGCTCCTGGAGCAGCTAAAGG
  TGTCTGCCCCTGCACGGGTGGTTAATGTGTCCTCGGTGGCTCACCACATTGGCAAGATTC
  CCTTCCACGACCTCCAGAGCGAGAAGCGCTACAGCAGGGGTTTTGCCTATTGCCACAGCA
  AGCTGGCCAATGTGCTTTTTACTCGTGAGCTGGCCAAGAGGCTCCAAGGCACCGGGGTCA
  CCACCTACGCAGTGCACCCAGGCGTCGTCCGCTCTGAGCTGGTCCGGCACTCCTCCCTGC
  TCTGCCTGCTCTGGCGGCTCTTCTCCCCCTTTGTCAAGACGGCACGGGAGGGGGCGCAGA
  CCAGCCTGCACTGCGCCCTGGCTGAGGGCCTGGAGCCCCTGAGTGGCAAGTACTTCAGTG
  ACTGCAAGAGGACCTGGGTGTCTCCAAGGGCCCGAAATAACAAAACAGCTGAGCGCCTAT
  GGAATGTCAGCTGTGAGCTTCTAGGAATCCGGTGGGAGTAGCTGGTGGAAGAGCTGCAGC
  TTTATCAGGCCCAATCCATGCCATAATGAACAGGGACCAAGGAGAAGGCCAACCCTAAAG
  GATTGTCCTCTTGGCCAGCTGGTGCTGCGAATCCTGCCTGCTCTGATCCTCTTGACCCTT
  CTGGGAATGTTTGCACACCTGACACTCTTGTGAGACTGGCTTATGGCATGAGTTGTGGAC
  ACCTATAGAGTGTTCTTCTCTAAGACCTGGAAAGTCAGCAACCCTCTGGGGGCAGCAGGA
  CTGGGCAGATCCCAGGCTGGGCATGGGGGTGGCAGAAGAGCCCGAGAAATTGGGTCAGTT
  CCCTCATCAGCACCAGAGGCTCAGCTGAGGCAAGAAGAGCACCATCACTGCCTATTTCTA
  GGGGCTATACACTCCAACTCTTGGTTGATCTCTTTCTTTTTAAAAATATTTGCCACCACC
  CTGGAGTCTAGACCAACACACAAAGATCCTGGCTAACCCTGGCCTATTTAGATTCCTTCC
  TCTCACCTGGACCTTCCCATTTCAATCATGCAGATGGTTTCTTTTTGTAAAGAGTTCCGT
  TTGCCTTTCAATTTTTAGAGAAAATAAAGACTGCATTCATCTCAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152443 unedited
  GGCTTTGGATTTGTATACGACTTACTATAGGCGGCCGCGTATTCANATCTGGTACCGGTC
  CGGAATTCCCGGGATAGGATTCTGGGCTCTGCTCAGAGTCAGGCTGCTGCTCAGCACCCA
  GGACGGAGAGGAGCAGAGAAGCAGCAGAAGCAGCCAAGAGCTGGAGCCAGACCAGGAACC
  TGAGCCAGAGCTGGGGTTGAAGCTGGAGCAGCAGCAAAAGCAACAGCAGCTACAGAAGTT
  GGAACGATGCTGGTCACCTTGGGACTGCTCACCTCCTTCTTCTCGTTCCTGTATATGGTA
  GCTCCATCCATCAGGAAGTTCTTTGCTGGTGGAGTGTGTAGAACAAATGTGCAGCTTCCT
  GGCAAGGTAGTGGTGATCACTGGCGCCAACACGGGCATTGGCAAGGAGACGGCCAGAGAG
  CTCGCTAGCCGAGGAGCCCGAGTCTATATTGCCTGCAGAGATGTACTGAAGGGGGAGTCT
  GCTGCCAGTGAAATCCGAGTGGATACAAAGAACTCCCAGGTGCTGGTGCGGAAATTGGAC
  CTATCCGACACCAAATCTATCCGAGCCTTTGCTGAGGGCTTTCTGGCAGAGGAAAAGCAG
  CTCCATATTCTGATCAACAATGCGGGAGTAATGATGTGTCCATATTCCAAGACAGCTGAT
  GGCTTTGAAACCCACCTGGGAGTCAACCACCTGGGCCACTTCCTCCTCACCTACCTGCTC
  CTGGAGCAGCTAAAGGTGTCTGCCCCTGCACGGGTGGTTAATGTGTCCTCGGTGGCTCAC
  CACATTGGCAAGATTCCCTTCCACGACCTNCAGAGCGAGAAGCGCTACAGCAGGGGTTTT
  GCCTATTGCCACAGCAAGCTGGCCAATGTGCTTTTTACTCGTGAGCTGGCCAAGAGGGCT
  CAAGGCACCGGGGTCACCACCTACGCAGTGCC
  
• Restriction Sites: Please inquire
• ACCN: NM_152443
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152443.1, NP_689656.1
• RefSeq Size: 1780 bp
• RefSeq ORF: 951 bp
• Locus ID: 145226
• UniProt ID: Q96NR8
• Cytogenetics: 14q24.1
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Retinol metabolism
• Gene Summary: The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in this gene are a cause of Leber congenital amaurosis type 13 and Retinitis Pigmentosa 53. [provided by RefSeq, Sep 2015]