Trypsin (PRSS3) (NM_002771) Human Untagged Clone

CAT#: SC125583

PRSS3 (untagged)-Human protease, serine, 3 (PRSS3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Trypsin
• Synonyms: MTG; PRSS4; T9; TRY3; TRY4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002771, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAATCCATTCCTGATCCTTGCCTTTGTGGGAGCTGCTGTTGCTGTCCCCTTTGACGATGATGACAAGA
  TTGTTGGGGGCTACACCTGTGAGGAGAATTCTCTCCCCTACCAGGTGTCCCTGAATTCTGGCTCCCACTT
  CTGCGGTGGCTCCCTCATCAGCGAACAGTGGGTGGTATCAGCAGCTCACTGCTACAAGACCCGCATCCAG
  GTGAGACTGGGAGAGCACAACATCAAAGTCCTGGAGGGGAATGAGCAGTTCATCAATGCGGCCAAGATCA
  TCCGCCACCCTAAATACAACAGGGACACTCTGGACAATGACATCATGCTGATCAAACTCTCCTCACCTGC
  CGTCATCAATGCCCGCGTGTCCACCATCTCTCTGCCCACCGCCCCTCCAGCTGCTGGCACTGAGTGCCTC
  ATCTCCGGCTGGGGCAACACTCTGAGCTTTGGTGCTGACTACCCAGACGAGCTGAAGTGCCTGGATGCTC
  CGGTGCTGACCCAGGCTGAGTGTAAAGCCTCCTACCCTGGAAAGATTACCAACAGCATGTTCTGTGTGGG
  CTTCCTTGAGGGAGGCAAGGATTCCTGCCAGCGTGACTCTGGTGGCCCTGTGGTCTGCAACGGACAGCTC
  CAAGGAGTTGTCTCCTGGGGCCATGGCTGTGCCTGGAAGAACAGGCCTGGAGTCTACACCAAGGTCTACA
  ACTATGTGGACTGGATTAAGGACACCATCGCTGCCAACAGCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002771 unedited
  ACATGATTTTGTAATACGCACTCATCTATAGGGCGCGCCGCGTAATTCAGTTGCTGGCTA
  CCGGCTCCGGTAATTCCCGGGATATCGTCGACCCACGCGTCCGAGCGGCGCGGGATGCAG
  ACGGCTGCGAGGCGCTGGGCACAGTTGCTGTCCCCTTTGACGATGATGACAAGATTGTTG
  GGGGCTACACCTGTGAGGAGAATTCTCTCCCCTACCAGGTGTCCCTGAATTCTGGCTCCC
  ACTTCTGCGGTGGCTCCCTCATCAGCGAACAGTGGGTGGTATCAGCAGCTCACTGCTACA
  AGACCCGCATCCAGGTGAGACTGGGAGAGCACAACATCAAAGTCCTGGAGGGGAATGAGC
  AGTTCATCAATGCGGCCAAGATCATCCGCCACCCTAAATACAACAGGGACACTCTGGACA
  ATGACATCATGCTGATCAAACTCTCCTCACCTGCCGTCATCAATGCCCGCGTGTCCACCA
  TCTCTCTGCCCACCGCCCCTCCAGCTGCTGGCACTGAGTGCCTCATCTCCGGCTGGGGCA
  ACACTCTGAGCTTTGGTGCTGACTACCCAGACGAGCTGAAGTGCCTGGATGCTCCGGTGC
  TGACCACGGCTGAGTGTAAAGCCTCCTACCCTGGAAAGATTACCAACAGCATGTTCTGTG
  TGGGCTTCCCATGAGGGAGGCAAGGATTCCCTGCCCAGCGTGACCTCTGGTGGCCCTGTG
  GTGCTGCACCGGACAGCTCCAAGGAATTGTCTACCGGGGGACAGGCTTGTGCCTGGAAAA
  AACAGGCCTTGGGATCTACACCGAGGTCCTACAACTTAGTGGGACTGGGTTAAAGAAAAG
  CNTGCGTGCCCACAGGTTAAAGCCCCGGGTCCCTTTGAGTTTTTTAACCATAAAAGGGGC
  CCGGCTCTTAAAAAAAAAAAAAAGGGGGCCGCGGCAATAATTGTTTCTGGAAAATACCCG
  GAGGGGTCCCCTTTGGCCCCTCCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002771
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002771.2, NP_002762.2
• RefSeq Size: 823 bp
• RefSeq ORF: 744 bp
• Locus ID: 5646
• UniProt ID: P35030
• Cytogenetics: 9p13.3
• Protein Families: Druggable Genome, Protease, Secreted Protein
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2010]
  Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1.