Desmocollin 2 (DSC2) (NM_024422) Human Untagged Clone

CAT#: SC124977

DSC2 (untagged)-Human desmocollin 2 (DSC2), transcript variant Dsc2a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Desmocollin 2
• Synonyms: ARVD11; CDHF2; DG2; DGII/III; DSC3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124977 sequence for NM_024422 edited (data generated by NextGen Sequencing)
  ATGGAGGCAGCCCGCCCCTCCGGCTCCTGGAACGGAGCCCTCTGCCGGCTGCTCCTGCTG
  ACCCTCGCGATCTTAATATTTGCCAGTGATGCCTGCAAAAATGTGACATTACATGTTCCC
  TCCAAACTAGATGCCGAGAAACTTGTTGGTAGAGTTAACCTGAAAGAGTGCTTTACAGCT
  GCAAATCTAATTCATTCAAGTGATCCTGACTTCCAAATTTTGGAGGATGGTTCAGTCTAT
  ACAACAAATACTATTCTATTGTCCTCGGAGAAGAGAAGTTTTACCATATTACTTTCCAAC
  ACTGAGAACCAAGAAAAGAAGAAAATATTTGTCTTTTTGGAGCATCAAACAAAGGTCCTA
  AAGAAAAGACATACTAAAGAAAAAGTTCTAAGGCGCGCCAAGAGAAGATGGGCTCCAATT
  CCTTGTTCGATGCTAGAAAACTCCTTGGGTCCTTTTCCACTTTTCCTTCAACAGGTTCAA
  TCTGACACGGCCCAAAACTATACCATATACTATTCCATAAGAGGTCCTGGAGTTGACCAA
  GAACCTCGGAATTTATTTTATGTGGAGAGAGACACTGGAAACTTGTATTGTACTCGTCCT
  GTAGATCGTGAGCAGTATGAATCTTTTGAGATAATTGCCTTTGCAACAACTCCAGATGGG
  TATACTCCAGAACTTCCACTGCCCCTAATAATCAAAATAGAGGATGAAAATGATAACTAC
  CCAATTTTTACAGAAGAAACTTATACTTTTACAATTTTTGAAAATTGCAGAGTGGGCACT
  ACTGTGGGACAAGTGTGTGCTACTGACAAAGATGAGCCTGACACGATGCACACACGCCTG
  AAGTACTCCATCATTGGGCAGGTGCCACCATCACCCACCCTATTTTCTATGCATCCAACT
  ACAGGCGTGATCACCACAACATCATCTCAGCTAGACAGAGAGTTAATTGACAAGTACCAG
  TTGAAAATAAAAGTACAAGACATGGATGGTCAGTATTTTGGTCTACAGACAACTTCAACT
  TGTATCATTAACATTGATGATGTAAATGACCACTTGCCAACATTTACTCGTACTTCTTAT
  GTGACATCAGTGGAAGAAAATACAGTTGATGTGGAAATCTTACGAGTTACTGTTGAGGAT
  AAGGACTTAGTGAATACTGCTAACTGGAGAGCTAATTATACCATTTTAAAGGGCAATGAA
  AATGGCAATTTTAAAATTGTAACAGATGCCAAAACCAATGAAGGAGTTCTTTGTGTAGTT
  AAGCCTTTGAATTATGAAGAAAAGCAACAGATGATCTTGCAAATTGGTGTAGTTAATGAA
  GCTCCATTTTCCAGAGAGGCTAGTCCAAGATCAGCCATGAGCACAGCAACAGTTACTGTT
  AATGTAGAAGATCAGGATGAGGGCCCTGAGTGTAACCCTCCAATACAGACTGTTCGCATG
  AAAGAAAATGCAGAAGTGGGAACAACAAGCAATGGATATAAAGCATATGACCCAGAAACA
  AGAAGTAGCAGTGGCATAAGGTATAAGAAATTAACTGATCCAACAGGGTGGGTCACCATT
  GATGAAAATACAGGATCAATCAAAGTTTTCAGAAGCCTGGATAGAGAGGCAGAGACCATC
  AAAAATGGCATATATAATATTACAGTCCTTGCATCAGACCAAGGAGGGAGAACATGTACG
  GGGACACTGGGCATTATACTTCAAGACGTGAATGATAACAGCCCATTCATACCTAAAAAG
  ACAGTGATCATCTGCAAACCCACCATGTCATCTGCGGAGATTGTTGCGGTTGATCCTGAT
  GAGCCTATCCATGGCCCACCCTTTGACTTTAGTCTGGAGAGTTCTACTTCAGAAGTACAG
  AGAATGTGGAGACTGAAAGCAATTAATGATACAGCAGCACGTCTTTCCTATCAGAATGAT
  CCTCCATTTGGCTCATATGTAGTACCTATAACAGTGAGAGATAGACTTGGCATGTCTAGT
  GTCACTTCATTGGATGTTACACTGTGTGACTGCATTACCGAAAATGACTGCACACATCGT
  GTAGATCCAAGGATTGGCGGTGGAGGAGTACAACTTGGAAAGTGGGCCATCCTTGCAATA
  TTGTTGGGCATAGCATTGCTCTTTTGCATCCTGTTTACGCTGGTCTGTGGGGCTTCTGGG
  ACGTCTAAACAACCAAAAGTAATTCCTGATGATTTAGCCCAGCAGAACCTAATTGTATCA
  AACACAGAAGCTCCTGGAGATGACAAAGTGTATTCTGCGAATGGCTTCACAACCCAAACT
  GTGGGCGCTTCTGCTCAGGGAGTTTGTGGCACCGTGGGATCAGGAATCAAAAACGGAGGT
  CAGGAGACCATCGAAATGGTGAAAGGAGGACACCAGACCTCGGAATCCTGCCGGGGGGCT
  GGCCACCATCACACCCTGGACTCCTGCAGGGGAGGACACACGGAGGTGGACAACTGCAGA
  TACACTTACTCGGAGTGGCACAGTTTTACTCAGCCCCGTCTTGGTGAAAAAGTGTATCTG
  TGTAATCAAGATGAAAATCACAAGCATGCCCAAGACTATGTCCTGACATATAACTATGAA
  GGAAGAGGATCGGTGGCTGGGTCTGTAGGTTGTTGCAGTGAACGACAAGAAGAAGATGGG
  CTTGAATTTTTGGATAATTTGGAGCCCAAATTTAGGACACTAGCAGAAGCATGCATGAAG
  AGATGA
  
  Clone variation with respect to NM_024422.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024422 unedited
  GGCAGCAATTTGTAATACGACTCACTATAGGGCGGCCGCGAAATCGGCACCAGGNATGAG
  AGGCAGGCGCTTCAGAGAAGCTAAGAAAACACCTCTCCGCGCGCCCCACCTCCTCCGCCT
  CGCGCTCCTCCTGAGCAGCGGGCCCAGACTGCGCTCCGGCCGCGGCCCTCGCCCCGCGGA
  GCCCTCCTACCCCGGCCCGACGCTCGGCCCGCGACCTGCCCCGAGCCCTCTCCATGGAGG
  CAGCCCGCCCCTCCGGCTCCTGGAACGGAGCCCTCTGCCGGCTGCTCCTGCTGACCCTCG
  CGATCTTAATATTTGCCAGTGATGCCTGCAAAAATGTGACATTACATGTTCCCTCCAAAC
  TAGATGCCGAGAAACTTGTTGGTAGAGTTAACCTGAAAGAGTGCTTTACAGCTGCAAATC
  TAATTCATTCAAGTGATCCTGACTTCCAAATTTTGGAGGATGGTTCAGTCTATACAACAA
  ATACTATTCTATTGTCCTCGGAGAAGAGAAGTTTTACCATATTACTTTCCAACACTGAGA
  ACCAAGAAAAGAAGAAAATATTTGTCTTTTTGGAGCATCAAACAAAGGTCCTAAAGAAAA
  GACATACTAAAGAAAAAGTTCTAAGGCGCGCCAAGAGAAGATGGGCTCCAATTCCTTGTT
  CGATGCTAGAAAACTCCTTGGGTCCTTTTCCACTTTTCCTTCAACAGGTTCAATCTGACA
  CGGCCCAAAACTATACCATATACTATTCCATAAGAGGTCCTGGAGTTGACCAAGAACCTC
  GGAATTTATTTTATGTGGAGAGAGAACTGNNGAACTGTATTGTACTCGTCCTGTAGATCG
  TGAGCAGTATGAAATCTTTGAGATATNGCCCTTGCACAACTCAGATGGTATCTNCAGAAC
  TTCATGNCCNNTATATCAATAGAGATGAAATGATACTACCCATTTTACAGAGAAACTAAC
  TT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_024422 unedited
  GGGGCTACTCGGGTCCCGAACCCAAATAGTTTTTNCAATAACACCCAGCGCCAAACACCG
  GAATATATCGGAANGACACCAACAGTGGCCCTTAGTGGACCAAAAAGAATGACTGTCATG
  CTTCAAAAATAATTTTTATTGAAGTGACTTACACTGGAGCCTGATGAAACAAGTTCTGTT
  ATTATCCCCCATTTTAAAATGATGAAAATGGACTTATCAAAAGCAAGCAACTTAACCTTT
  AGGGGGGGGCCTCACAGCCTTTAGAATAGTCATATTATATAAATATGGCAATAACAATGC
  ACTGAAAATGTCTCCCAAACACACTCTACTCTTTTAAAAAATGTATAACAGGCAATCTAA
  GGAAGGGGTCTTACTTCTCTGATTCACGGAGTGCTAAATCCCTTAAACTCAAATCAGACC
  TGTGATGAGCAAGCTGCAACAAAGTTACAGAAACTGCTTAACTTACTTTATCATGGCCTA
  TTATTGTATACGAATTTCTTATTTACTTTACTAAACACAACTTTGGAAAACTAATTTAGG
  CTGTCCGGCATAAAACTTCCGGTAAGATAACCTGCTCACCACACAACTCCGTAGAGATAA
  CCTGGCCGACAATTGTTCGAACAGCGTCGGGGACCTTAACACCCGCCCCGTCGCCGCCGT
  TCAAATAAGTAGGAGCCGCTTTAACCTCTGCCTTATGTTCACTATAAATAGAAAACTCTT
  TGCACCACCATTCTCGCGAAACATCGCCCGGACCTATCGCGAGCCGCCACGCGAACGTTC
  GATTCACGCGCCCACCAGTCACAGCGTTGGCCGCCGTGCCCCCGCGTCTCTTCGCTGCAG
  TCTAAGACGAAATCCACAGCCGTCACCCCCGCCCCGGCCCCACACCACCCATCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_024422
• Insert Size: 4700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024422.2, NP_077740.1
• RefSeq Size: 5140 bp
• RefSeq ORF: 2706 bp
• Locus ID: 1824
• UniProt ID: Q02487
• Cytogenetics: 18q12.1
• Domains: CA
• Protein Families: Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• Gene Summary: This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]
  Transcript Variant: This variant (Dsc2a, also known as DGII) encodes the longer isoform (Dsc2a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.