Desmocollin 2 (DSC2) (NM_024422) Human Tagged ORF Clone

CAT#: RC209218

DSC2 (Myc-DDK-tagged)-Human desmocollin 2 (DSC2), transcript variant Dsc2a

Product Images

Circular map for RC209218

• 

  Coomassie blue staining of purified DSC2 protein (Cat# TP309218). The protein was produced from HEK293T cells transfected with DSC2 cDNA clone (Cat# RC209218) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Desmocollin 2
• Synonyms: ARVD11; CDHF2; DG2; DGII/III; DSC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209218 representing NM_024422
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCAGCCCGCCCCTCCGGCTCCTGGAACGGAGCCCTCTGCCGGCTGCTCCTGCTGACCCTCGCGA
  TCTTAATATTTGCCAGTGATGCCTGCAAAAATGTGACATTACATGTTCCCTCCAAACTAGATGCCGAGAA
  ACTTGTTGGTAGAGTTAACCTGAAAGAGTGCTTTACAGCTGCAAATCTAATTCATTCAAGTGATCCTGAC
  TTCCAAATTTTGGAGGATGGTTCAGTCTATACAACAAATACTATTCTATTGTCCTCGGAGAAGAGAAGTT
  TTACCATATTACTTTCCAACACTGAGAACCAAGAAAAGAAGAAAATATTTGTCTTTTTGGAGCATCAAAC
  AAAGGTCCTAAAGAAAAGACATACTAAAGAAAAAGTTCTAAGGCGCGCCAAGAGAAGATGGGCTCCAATT
  CCTTGTTCGATGCTAGAAAACTCCTTGGGTCCTTTTCCACTTTTCCTTCAACAGGTTCAATCTGACACGG
  CCCAAAACTATACCATATACTATTCCATAAGAGGTCCTGGAGTTGACCAAGAACCTCGGAATTTATTTTA
  TGTGGAGAGAGACACTGGAAACTTGTATTGTACTCGTCCTGTAGATCGTGAGCAGTATGAATCTTTTGAG
  ATAATTGCCTTTGCAACAACTCCAGATGGGTATACTCCAGAACTTCCACTGCCCCTAATAATCAAAATAG
  AGGATGAAAATGATAACTACCCAATTTTTACAGAAGAAACTTATACTTTTACAATTTTTGAAAATTGCAG
  AGTGGGCACTACTGTGGGACAAGTGTGTGCTACTGACAAAGATGAGCCTGACACGATGCACACACGCCTG
  AAGTACTCCATCATTGGGCAGGTGCCACCATCACCCACCCTATTTTCTATGCATCCAACTACAGGCGTGA
  TCACCACAACATCATCTCAGCTAGACAGAGAGTTAATTGACAAGTACCAGTTGAAAATAAAAGTACAAGA
  CATGGATGGTCAGTATTTTGGTCTACAGACAACTTCAACTTGTATCATTAACATTGATGATGTAAATGAC
  CACTTGCCAACATTTACTCGTACTTCTTATGTGACATCAGTGGAAGAAAATACAGTTGATGTGGAAATCT
  TACGAGTTACTGTTGAGGATAAGGACTTAGTGAATACTGCTAACTGGAGAGCTAATTATACCATTTTAAA
  GGGCAATGAAAATGGCAATTTTAAAATTGTAACAGATGCCAAAACCAATGAAGGAGTTCTTTGTGTAGTT
  AAGCCTTTGAATTATGAAGAAAAGCAACAGATGATCTTGCAAATTGGTGTAGTTAATGAAGCTCCATTTT
  CCAGAGAGGCTAGTCCAAGATCAGCCATGAGCACAGCAACAGTTACTGTTAATGTAGAAGATCAGGATGA
  GGGCCCTGAGTGTAACCCTCCAATACAGACTGTTCGCATGAAAGAAAATGCAGAAGTGGGAACAACAAGC
  AATGGATATAAAGCATATGACCCAGAAACAAGAAGTAGCAGTGGCATAAGGTATAAGAAATTAACTGATC
  CAACAGGGTGGGTCACCATTGATGAAAATACAGGATCAATCAAAGTTTTCAGAAGCCTGGATAGAGAGGC
  AGAGACCATCAAAAATGGCATATATAATATTACAGTCCTTGCATCAGACCAAGGAGGGAGAACATGTACG
  GGGACACTGGGCATTATACTTCAAGACGTGAATGATAACAGCCCATTCATACCTAAAAAGACAGTGATCA
  TCTGCAAACCCACCATGTCATCTGCGGAGATTGTTGCGGTTGATCCTGATGAGCCTATCCATGGCCCACC
  CTTTGACTTTAGTCTGGAGAGTTCTACTTCAGAAGTACAGAGAATGTGGAGACTGAAAGCAATTAATGAT
  ACAGCAGCACGTCTTTCCTATCAGAATGATCCTCCATTTGGCTCATATGTAGTACCTATAACAGTGAGAG
  ATAGACTTGGCATGTCTAGTGTCACTTCATTGGATGTTACACTGTGTGACTGCATTACCGAAAATGACTG
  CACACATCGTGTAGATCCAAGGATTGGCGGTGGAGGAGTACAACTTGGAAAGTGGGCCATCCTTGCAATA
  TTGTTGGGCATAGCATTGCTCTTTTGCATCCTGTTTACGCTGGTCTGTGGGGCTTCTGGGACGTCTAAAC
  AACCAAAAGTAATTCCTGATGATTTAGCCCAGCAGAACCTAATTGTATCAAACACAGAAGCTCCTGGAGA
  TGACAAAGTGTATTCTGCGAATGGCTTCACAACCCAAACTGTGGGCGCTTCTGCTCAGGGAGTTTGTGGC
  ACCGTGGGATCAGGAATCAAAAACGGAGGTCAGGAGACCATCGAAATGGTGAAAGGAGGACACCAGACCT
  CGGAATCCTGCCGGGGGGCTGGCCACCATCACACCCTGGACTCCTGCAGGGGAGGACACACGGAGGTGGA
  CAACTGCAGATACACTTACTCGGAGTGGCACAGTTTTACTCAGCCCCGTCTTGGTGAAAAAGTGTATCTG
  TGTAATCAAGATGAAAATCACAAGCATGCCCAAGACTATGTCCTGACATATAACTATGAAGGAAGAGGAT
  CGGTGGCTGGGTCTGTAGGTTGTTGCAGTGAACGACAAGAAGAAGATGGGCTTGAATTTTTGGATAATTT
  GGAGCCCAAATTTAGGACACTAGCAGAAGCATGCATGAAGAGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209218 representing NM_024422
  Red=Cloning site Green=Tags(s)
  
  MEAARPSGSWNGALCRLLLLTLAILIFASDACKNVTLHVPSKLDAEKLVGRVNLKECFTAANLIHSSDPD
  FQILEDGSVYTTNTILLSSEKRSFTILLSNTENQEKKKIFVFLEHQTKVLKKRHTKEKVLRRAKRRWAPI
  PCSMLENSLGPFPLFLQQVQSDTAQNYTIYYSIRGPGVDQEPRNLFYVERDTGNLYCTRPVDREQYESFE
  IIAFATTPDGYTPELPLPLIIKIEDENDNYPIFTEETYTFTIFENCRVGTTVGQVCATDKDEPDTMHTRL
  KYSIIGQVPPSPTLFSMHPTTGVITTTSSQLDRELIDKYQLKIKVQDMDGQYFGLQTTSTCIINIDDVND
  HLPTFTRTSYVTSVEENTVDVEILRVTVEDKDLVNTANWRANYTILKGNENGNFKIVTDAKTNEGVLCVV
  KPLNYEEKQQMILQIGVVNEAPFSREASPRSAMSTATVTVNVEDQDEGPECNPPIQTVRMKENAEVGTTS
  NGYKAYDPETRSSSGIRYKKLTDPTGWVTIDENTGSIKVFRSLDREAETIKNGIYNITVLASDQGGRTCT
  GTLGIILQDVNDNSPFIPKKTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWRLKAIND
  TAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTHRVDPRIGGGGVQLGKWAILAI
  LLGIALLFCILFTLVCGASGTSKQPKVIPDDLAQQNLIVSNTEAPGDDKVYSANGFTTQTVGASAQGVCG
  TVGSGIKNGGQETIEMVKGGHQTSESCRGAGHHHTLDSCRGGHTEVDNCRYTYSEWHSFTQPRLGEKVYL
  CNQDENHKHAQDYVLTYNYEGRGSVAGSVGCCSERQEEDGLEFLDNLEPKFRTLAEACMKR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024422
• ORF Size: 2703 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024422.6
• RefSeq Size: 5140 bp
• RefSeq ORF: 2706 bp
• Locus ID: 1824
• UniProt ID: Q02487
• Cytogenetics: 18q12.1
• Domains: CA
• Protein Families: Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)
• MW: 97 kDa
• Gene Summary: This gene encodes a member of the desmocollin protein subfamily. Desmocollins, along with desmogleins, are cadherin-like transmembrane glycoproteins that are major components of the desmosome. Desmosomes are cell-cell junctions that help resist shearing forces and are found in high concentrations in cells subject to mechanical stress. This gene is found in a cluster with other desmocollin family members on chromosome 18. Mutations in this gene are associated with arrhythmogenic right ventricular dysplasia-11, and reduced protein expression has been described in several types of cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2015]