WDR48 (NM_020839) Human Untagged Clone

CAT#: SC124415

WDR48 (untagged)-Human WD repeat domain 48 (WDR48)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WDR48
• Synonyms: Bun62; P80; SPG60; UAF1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124415 sequence for NM_020839 edited (data generated by NextGen Sequencing)
  ATGGCGGCCCATCACCGGCAGAACACAGCAGGGCGGAGGAAAGTGCAGGTTTCCTATGTT
  ATTCGAGATGAAGTGGAGAAGTACAACCGAAATGGAGTCAATGCTCTGCAGCTGGATCCA
  GCACTAAATAGACTTTTCACAGCCGGTCGAGACTCTATCATAAGAATATGGAGTGTCAAT
  CAGCACAAGCAAGATCCATATATAGCATCTATGGAACACCATACTGATTGGGTAAACGAC
  ATTGTACTCTGTTGTAATGGGAAAACATTAATATCTGCTTCTTCTGACACGACAGTAAAA
  GTATGGAATGCACACAAGGGATTTTGCATGTCAACATTAAGGACACATAAGGATTACGTA
  AAGGCCTTAGCATATGCCAAGGATAAAGAACTAGTAGCATCAGCTGGGTTGGACAGACAA
  ATATTCCTTTGGGATGTGAATACTCTAACAGCATTGACTGCCTCAAATAACACTGTCACA
  ACTTCTTCTTTAAGTGGAAACAAAGATTCCATTTATAGCCTGGCCATGAATCAACTGGGA
  ACAATCATTGTATCAGGGTCCACTGAAAAGGTGTTACGGGTATGGGATCCAAGAACATGT
  GCAAAACTAATGAAGCTTAAAGGGCACACGGATAATGTGAAGGCATTGCTATTAAACAGA
  GATGGCACGCAATGCCTGTCAGGCAGTTCTGATGGGACAATTCGCCTTTGGTCCCTTGGC
  CAGCAGAGATGTATAGCAACATACCGAGTCCATGATGAAGGTGTTTGGGCGCTGCAAGTC
  AATGATGCCTTCACACATGTGTATTCTGGTGGAAGGGACAGGAAGATTTATTGTACAGAC
  CTAAGAAACCCTGACATTCGGGTGCTAATTTGTGAAGAAAAAGCACCAGTTCTCAAGATG
  GAGCTTGATAGATCAGCTGATCCTCCTCCTGCAATTTGGGTTGCAACAACTAAGTCTACA
  GTAAATAAATGGACTTTGAAAGGAATTCATAATTTTAGAGCCTCTGGAGATTATGACAAT
  GACTGTACAAATCCTATAACACCTCTTTGTACACAACCTGACCAGGTTATTAAAGGGGGT
  GCTAGTATTATTCAGTGCCACATTCTTAATGATAAGAGACATATATTAACCAAAGATACC
  AATAATAATGTGGCATATTGGGATGTATTGAAGGCATGTAAAGTTGAAGATCTGGGCAAA
  GTGGATTTTGAAGATGAAATTAAGAAAAGATTTAAAATGGTGTATGTGCCAAATTGGTTC
  TCAGTAGACTTAAAAACAGGGATGTTAACTATTACTTTGGATGAAAGTGATTGTTTTGCT
  GCCTGGGTTTCTGCAAAAGATGCTGGTTTCAGCAGCCCTGATGGGTCAGATCCAAAATTG
  AATTTAGGAGGACTTTTACTCCAAGCACTCCTGGAATATTGGCCTAGAACACATGTGAAT
  CCAATGGATGAAGAGGAAAATGAAGTAAACCATGTAAATGGGGAGCAGGAGAACCGAGTG
  CAGAAGGGAAATGGATATTTTCAAGTGCCCCCACATACACCCGTGATCTTTGGTGAAGCT
  GGAGGTCGCACACTGTTCAGGCTGCTCTGCCGAGATTCCGGGGGTGAGACTGAGTCTATG
  CTTCTTAATGAAACAGTGCCACAATGGGTAATTGACATCACTGTGGATAAAAATATGCCC
  AAATTCAACAAAATTCCTTTCTACCTCCAACCTCATGCATCTTCAGGAGCAAAAACCTTA
  AAAAAAGATAGACTCTCTGCTAGTGACATGCTCCAAGTCCGAAAAGTTATGGAACATGTT
  TATGAAAAAATTATCAACTTGGATAATGAGTCTCAAACCACTAGCTCTTCTAATAATGAA
  AAACCAGGAGAACAGGAAAAAGAAGAAGATATTGCTGTGTTGGCAGAGGAGAAAATTGAA
  CTTTTGTGCCAGGACCAGGTTTTGGATCCAAATATGGACCTTCGAACAGTGAAACACTTC
  ATATGGAAGAGCGGTGGAGACCTCACCCTCCATTACCGTCAGAAGTCCACGTGA
  
  Clone variation with respect to NM_020839.2
  1932 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_020839 unedited
  GCGGAATTCGCACGAGGCAACATGCAAGATGGCGGCCCATCACCGGCAGAACACAGCAGG
  GCGGAGGAAAGTGCAGGTTTCCTATGTTATTCGAGATGAAGTGGAGAAGTACAACCGAAA
  TGGAGTCAATGCTCTGCAGCTGGATCCAGCACTAAATAGACTTTTCACAGCCGGTCGAGA
  CTCTATCATAAGAATATGGAGTGTCAATCAGCACAAGCAAGATCCATATATAGCATCTAT
  GGAACACCATACTGATTGGGTAAACGACATTGTACTCTGTTGTAATGGGAAAACATTAAT
  ATCTGCTTCTTCTGACACGACAGTAAAAGTATGGAATGCACACAAGGGATTTTGCATGTC
  AACATTAAGGACACATAAGGATTACGTAAAGGCCTTAGCATATGCCAAGGATAAAGAACT
  AGTAGCATCAGCTGGGTTGGACAGACAAATATTCCTTTGGGATGTGAATACTCTAACAGC
  ATTGACTGCCTCAAATAACACTGTCACAACTTCTTCTTTAAGTGGAAACAAAGATTCCAT
  TTATAGCCTGGCCATGAATCAACTGGGAACAATCATTGTATCAGGGTCCACTGAAAAGGT
  GTTACGGGTATGGGATCCAAGAACATGTGCAAAACTAATGAAGCTTANAGGGCACACGGA
  TAATGTGAAGGCATTGCTATTAAACAGAGATGGCACGCCATGCCTGTCANGCAGTTCTGA
  TGGGACAATTCGCCCTTGGGTCCCTTGGCCAGCAGAGATGTATAGCCACATACCGAGTCC
  ATGATGAAAGGTGTTTGGGCGCTGCCAGTCAATGATGCCTTCCCACATGTGTATTCTGGT
  GGAAAGGCACAGGAGTATTTATGGTCAGACCTTAGAAACCTGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_020839 unedited
  TACTACTAAGNAACCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTATTTT
  AACTAAAGCCTTTATTCTATACAACTGTNGAAATACAGATTTTTACCCTTTTGGCATTGC
  AGACTGTCGAATTTTCTGGAAACTGTATTACGGTTTGACTGGAGGGGAAAGAAATACAAT
  CATGCTGGGGGTCAGATCTATGACAGGAGGGGAATGCAGCCACCCAAGGCTTAGCACACA
  GGGCAAAATGACCCAGCAAAACCCATTTCTTTGCTCAAAGGCCCCTCGGGCAAACACAAC
  TGAGGGCTGGTAAATAATTTTTTTTTTCTTTGCCATTAATTCACAACTATTGCTAAAAAA
  TGTGCACCAAAAGCACACTGTTGTATCCAACAGATAGAAAGGACTTTTAAAAAAAAAGAA
  GCTGGTTGCTCTCTGAGGAAGGGAGAAAGACCATTTCAACTTATTAGAAATGTGGCCACG
  TCTTTGCTCACTTCTTCAAAGTGCAGGTGGCAATGGCCTGTCCAGTTTCCAAAAGAAGCT
  GACTAGCAGTGAGGGAACCCTGTCTCTGCAGTTGTGCGATCAGAGTATCTTAAATAGTAA
  GGTATGGCCTTAGGCCTATCAGCTGCCCCCAGCCGAACCTGTGAAGATGTAGAAAAGAAA
  AAACGTTCCCTGCCTCCCTCCCTAGTCCCTGCTATGGTGGAGGCAGGCCACACACAAGCT
  AGGGAGGCAACTGGACTGGAATCAACTGCCCTCTTGAAGCACTTGTGCTTCTGGCTTCAT
  ACAGNGAGCTTGTCTTACAGTTTTATGGGGGTGGACCACTGATTTACTCTGAATACAAAT
  GACCTTGGGNAGAATCCAAGTATTTTCTGCCCAAATTGAAGTGTTATCCCCATAAGTACA
  CTCTAACAAGATCCAATGATTCTTGAAAGTGTTAGATGGTAGAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_020839
• Insert Size: 4500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020839.2, NP_065890.1
• RefSeq Size: 3705 bp
• RefSeq ORF: 2034 bp
• Locus ID: 57599
• UniProt ID: Q8TAF3
• Cytogenetics: 3p22.2
• Domains: WD40
• Gene Summary: The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).