WDR48 (NM_020839) Human Tagged ORF Clone

CAT#: RC205497

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WDR48 (Myc-DDK-tagged)-Human WD repeat domain 48 (WDR48)



  "NM_020839" in other vectors (6)

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


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In Stock

Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol WDR48
Synonyms Bun62; P80; SPG60; UAF1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC205497 representing NM_020839
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCCATCACCGGCAGAACACAGCAGGGCGGAGGAAAGTGCAGGTTTCCTATGTTATTCGAGATG
AAGTGGAGAAGTACAACCGAAATGGAGTCAATGCTCTGCAGCTGGATCCAGCACTAAATAGACTTTTCAC
AGCCGGTCGAGACTCTATCATAAGAATATGGAGTGTCAATCAGCACAAGCAAGATCCATATATAGCATCT
ATGGAACACCATACTGATTGGGTAAACGACATTGTACTCTGTTGTAATGGGAAAACATTAATATCTGCTT
CTTCTGACACGACAGTAAAAGTATGGAATGCACACAAGGGATTTTGCATGTCAACATTAAGGACACATAA
GGATTACGTAAAGGCCTTAGCATATGCCAAGGATAAAGAACTAGTAGCATCAGCTGGGTTGGACAGACAA
ATATTCCTTTGGGATGTGAATACTCTAACAGCATTGACTGCCTCAAATAACACTGTCACAACTTCTTCTT
TAAGTGGAAACAAAGATTCCATTTATAGCCTGGCCATGAATCAACTGGGAACAATCATTGTATCAGGGTC
CACTGAAAAGGTGTTACGGGTATGGGATCCAAGAACATGTGCAAAACTAATGAAGCTTAAAGGGCACACG
GATAATGTGAAGGCATTGCTATTAAACAGAGATGGCACGCAATGCCTGTCAGGCAGTTCTGATGGGACAA
TTCGCCTTTGGTCCCTTGGCCAGCAGAGATGTATAGCAACATACCGAGTCCATGATGAAGGTGTTTGGGC
GCTGCAAGTCAATGATGCCTTCACACATGTGTATTCTGGTGGAAGGGACAGGAAGATTTATTGTACAGAC
CTAAGAAACCCTGACATTCGGGTGCTAATTTGTGAAGAAAAAGCACCAGTTCTCAAGATGGAGCTTGATA
GATCAGCTGATCCTCCTCCTGCAATTTGGGTTGCAACAACTAAGTCTACAGTAAATAAATGGACTTTGAA
AGGAATTCATAATTTTAGAGCCTCTGGAGATTATGACAATGACTGTACAAATCCTATAACACCTCTTTGT
ACACAACCTGACCAGGTTATTAAAGGGGGTGCTAGTATTATTCAGTGCCACATTCTTAATGATAAGAGAC
ATATATTAACCAAAGATACCAATAATAATGTGGCATATTGGGATGTATTGAAGGCATGTAAAGTTGAAGA
TCTGGGCAAAGTGGATTTTGAAGATGAAATTAAGAAAAGATTTAAAATGGTGTATGTGCCAAATTGGTTC
TCAGTAGACTTAAAAACAGGGATGTTAACTATTACTTTGGATGAAAGTGATTGTTTTGCTGCCTGGGTTT
CTGCAAAAGATGCTGGTTTCAGCAGCCCTGATGGGTCAGATCCAAAATTGAATTTAGGAGGACTTTTACT
CCAAGCACTCCTGGAATATTGGCCTAGAACACATGTGAATCCAATGGATGAAGAGGAAAATGAAGTAAAC
CATGTAAATGGGGAGCAGGAGAACCGAGTGCAGAAGGGAAATGGATATTTTCAAGTGCCCCCACATACAC
CCGTGATCTTTGGTGAAGCTGGAGGTCGCACACTGTTCAGGCTGCTCTGCCGAGATTCCGGGGGTGAGAC
TGAGTCTATGCTTCTTAATGAAACAGTGCCACAATGGGTAATTGACATCACTGTGGATAAAAATATGCCC
AAATTCAACAAAATTCCTTTCTACCTCCAACCTCATGCATCTTCAGGAGCAAAAACCTTAAAAAAAGATA
GACTCTCTGCTAGTGACATGCTCCAAGTCCGAAAAGTTATGGAACATGTTTATGAAAAAATTATCAACTT
GGATAATGAGTCTCAAACCACTAGCTCTTCTAATAATGAAAAACCAGGAGAACAGGAAAAAGAAGAAGAT
ATTGCTGTGTTGGCAGAGGAGAAAATTGAACTTTTGTGCCAAGACCAGGTTTTGGATCCAAATATGGACC
TTCGAACAGTGAAACACTTCATATGGAAGAGCGGTGGAGACCTCACCCTCCATTACCGTCAGAAGTCCAC
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC205497 representing NM_020839
Red=Cloning site Green=Tags(s)

MAAHHRQNTAGRRKVQVSYVIRDEVEKYNRNGVNALQLDPALNRLFTAGRDSIIRIWSVNQHKQDPYIAS
MEHHTDWVNDIVLCCNGKTLISASSDTTVKVWNAHKGFCMSTLRTHKDYVKALAYAKDKELVASAGLDRQ
IFLWDVNTLTALTASNNTVTTSSLSGNKDSIYSLAMNQLGTIIVSGSTEKVLRVWDPRTCAKLMKLKGHT
DNVKALLLNRDGTQCLSGSSDGTIRLWSLGQQRCIATYRVHDEGVWALQVNDAFTHVYSGGRDRKIYCTD
LRNPDIRVLICEEKAPVLKMELDRSADPPPAIWVATTKSTVNKWTLKGIHNFRASGDYDNDCTNPITPLC
TQPDQVIKGGASIIQCHILNDKRHILTKDTNNNVAYWDVLKACKVEDLGKVDFEDEIKKRFKMVYVPNWF
SVDLKTGMLTITLDESDCFAAWVSAKDAGFSSPDGSDPKLNLGGLLLQALLEYWPRTHVNPMDEEENEVN
HVNGEQENRVQKGNGYFQVPPHTPVIFGEAGGRTLFRLLCRDSGGETESMLLNETVPQWVIDITVDKNMP
KFNKIPFYLQPHASSGAKTLKKDRLSASDMLQVRKVMEHVYEKIINLDNESQTTSSSNNEKPGEQEKEED
IAVLAEEKIELLCQDQVLDPNMDLRTVKHFIWKSGGDLTLHYRQKST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_020839
ORF Size 2031 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_020839.2, NP_065890.1
RefSeq Size 3705 bp
RefSeq ORF 2034 bp
Locus ID 57599
UniProt ID Q8TAF3
Cytogenetics 3p22.2
Domains WD40
MW 76 kDa
Gene Summary The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016]

Other Versions

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