RHBDF2 (NM_024599) Human Untagged Clone

CAT#: SC122961

RHBDF2 (untagged)-Human rhomboid 5 homolog 2 (Drosophila) (RHBDF2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RHBDF2
• Synonyms: iRhom2; RHBDL5; RHBDL6; TEC; TOC; TOCG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_024599 edited
  GCCGCTCGCAGGCGCCGCACGGAGTTGCGTCCCGGGGACTTGGGGCCGCAGGGAGCTGTG
  AGTACCCAGGAAGCTGCACCGTGTGGCCTGGAGCTGTCTATCTGTCCTTCCAGCCACCTG
  TCTGTCCAGCCACCCTTCCACAGACTGAGGCTTGACACCGGAGCATCTGTACAGAGCAAG
  GAGAAGACAAGAACATGCTCTAAAGCCCTTCACAGCAAGACCCAGGAAGCCGCGGGCAAA
  CTCAGACTCGAAGCCCTCCCGCCTCCTGCCCACAATGGCCTCTGCTGACAAGAATGGCGG
  GAGCGTGTCCTCTGTGTCCAGCAGCCGCCTGCAGAGCCGGAAGCCACCCAACCTCTCCAT
  CACCATCCCGCCACCCGAGAAAGAGACCCAGGCCCCTGGCGAGCAGGACAGCATGCTGCC
  TGAGGGTTTTCAGAATAGGAGGCTAAAGAAAAGCCAGCCCAGGACCTGGGCTTCACACAC
  CACCGCCTGCCCTCCCTCCTTCCTCCCCAAGAGGAAGAACCCAGCCTACTTGAAGAGCGT
  CAGCCTCCAGGAGCCACGCAGCCGATGGCAGGAGAGTTCAGAGAAGCGCCCTGGCTTCCG
  CCGCCAGGCCTCACTGTCCCAGAGCATCCGCAAGGGCGCAGCCCAGTGGTTTGGAGTCAG
  CGGCGACTGGGAGGGGCAGCGGCAGCAGTGGCAGCGCCGCAGCCTGCACCACTGCAGCAT
  GCGCTACGGCCGCCTGAAGGCCTCGTGCCAGCGTGACCTGGAGCTCCCCAGCCAGGAGGC
  ACCGTCCTTCCAGGGCACTGAGTCCCCAAAGCCCTGCAAGATGCCCAAGATTGTGGATCC
  GCTGGCCCGGGGCCGGGCCTTCCGCCACCCGGAGGAGATGGACAGGCCCCACGCCCTGCA
  CCCACCGCTGACCCCCGGAGTCCTGTCCCTCACCTCCTTCACCAGTGTCCGTTCTGGCTA
  CTCCCACCTGCCACGCCGCAAGAGAATGTCTGTGGCCCACATGAGCTTGCAAGCTGCCGC
  TGCCCTCCTCAAGGGGCGCTCGGTGCTGGATGCCACCGGACAGCGGTGCCGGGTGGTCAA
  GCGCAGCTTTGCCTTCCCGAGCTTCCTGGAGGAGGATGTGGTCGATGGGGCAGACACGTT
  TGACTCCTCCTTTTTTAGTAAGGAAGAAATGAGCTCCATGCCTGATGATGTCTTTGAGTC
  CCCCCCACTCTCTGCCAGCTACTTCCGAGGGATCCCACACTCAGCCTCCCCTGTCTCCCC
  CGATGGGGTGCAAATCCCTCTGAAGGAGTATGGCCGAGCCCCAGTCCCCGGGCCCCGGCG
  CGGCAAGCGCATCGCCTCCAAGGTGAAGCACTTTGCCTTTGATCGGAAGAAGCGGCACTA
  CGGCCTCGGCGTGGTGGGCAACTGGCTGAACCGCAGCTACCGCCGCAGCATCAGCAGCAC
  TGTGCAGCGGCAGCTGGAGAGCTTCGACAGCCACCGGCCCTACTTCACCTACTGGCTGAC
  CTTCGTCCATGTCATCATCACGCTGCTGGTGATTTGCACGTATGGCATCGCACCCGTGGG
  CTTTGCCCAGCACGTCACCACCCAGCTGGTGCTGCGGAACAAAGGTGTGTACGAGAGCGT
  GAAGTACATCCAGCAGGAGAACTTCTGGGTTGGCCCCAGCTCGATTGACCTGATCCACCT
  GGGGGCCAAGTTCTCACCCTGCATCCGGAAGGACGGGCAGATCGAGCAGCTGGTGCTGCG
  CGAGCGAGACCTGGAGCGGGACTCAGGCTGCTGTGTCCAGAATGACCACTCCGGATGCAT
  CCAGACCCAGCGGAAGGACTGCTCGGAGACTTTGGCCACTTTTGTCAAGTGGCAGGATGA
  CACTGGGCCCCCCATGGACAAGTCTGATCTGGGCCAGAAGCGGACTTCGGGGGCTGTCTG
  CCACCAGGACCCCAGGACCTGCGAGGAGCCAGCCTCCAGCGGTGCCCACATCTGGCCCGA
  TGACATCACTAAGTGGCCGATCTGCACAGAGCAGGCCAGGAGCAACCACACAGGCTTCCT
  GCACATGGACTGCGAGATCAAGGGCCGCCCCTGCTGCATCGGCACCAAGGGCAGCTGTGA
  GATCACCACCCGGGAATACTGTGAGTTCATGCACGGCTATTTCCATGAGGAAGCAACACT
  CTGCTCCCAGGTGCACTGCTTGGACAAGGTGTGTGGGCTGCTGCCCTTCCTCAACCCTGA
  GGTCCCAGATCAGTTCTACAGGCTCTGGCTGTCTCTCTTCCTACATGCTGGCGTGGTGCA
  CTGCCTCGTGTCTGTGGTCTTTCAAATGACCATCCTGAGGGACCTGGAGAAGCTGGCCGG
  CTGGCACCGTATCGCCATCATCTTCATCCTCAGTGGCATCACAGGCAACCTCGCCAGTGC
  CATCTTTCTCCCATACCGGGCAGAGGTGGGCCCGGCCGGCTCACAGTTCGGCCTCCTCGC
  CTGCCTCTTCGTGGAGCTCTTCCAGAGCTGGCCGCTGCTGGAGAGGCCCTGGAAGGCCTT
  CCTCAACCTCTCGGCCATCGTGCTCTTCCTGTTCATCTGTGGCCTCCTGCCCTGGATCGA
  CAACATCGCCCACATCTTCGGCTTCCTCAGTGGCCTGCTGCTGGCCTTCGCCTTCCTGCC
  CTACATCACCTTCGGCACCAGCGACAAGTACCGCAAGCGGGCACTCATCCTGGTGTCACT
  GCTGGCCTTTGCCGGCCTCTTCGCCGCCCTCGTGCTGTGGCTGTACATCTACCCCATTAA
  CTGGCCCTGGATCGAGCACCTCACCTGCTTCCCCTTCACCAGCCGCTTCTGCGAGAAGTA
  TGAGCTGGACCAGGTGCTGCACTGACCGCTGGGCCACACGGCTGCCCCTCAGCCCTGCTG
  GAACAGGGTCTGCCTGCGAGGGCTGCCCTCTGCAGAGCGCTCTCTGTGTGCCAGAGAGCC
  AGAGACCCAAGACAGGGCCCGGGCTCTGGACCTGGGTGCCCCCCTGCCAGGCGAGGCTGA
  CTCCGCGTGAGATGGTTGGTTAAGGCGGGGTTTTTCTGGGGCGTGAGGCCTGTGAGATCC
  TGACCCAAGCTCAGGCACACCCAAGGCACCTGCCTCTCTGAGTCTTGGGTCTCAGTTCCT
  AATATCCCGCTCCTTGCTGAGACCATCTCCTGGGGCAGGGTCCTTTTCTTCCCAGGTCCT
  CAGCGCTGCCTCTGCTGGTGCCTTCTCCCCCACTACTACTGGAGCGTGCCCTTGCTGGGG
  ACGTGGCTGTGCCCTCAGTTGCCCCCAGGGCTGGGTGCCCACCATGCCCCTTCCTCTTTC
  TCCTCCTACCTCTGCCCTGTGAGCCCATCCATAAGGCTCTCAGATGGGACATTGTGGGAA
  AGGCTTTGGCCATGGTCTGGGGGCAGAGAACAAGGGGGGAGACACAAGTAGACCTCAGGT
  AGAACGACACTGGGCGGAGCCACCCCAGGGCCTGCTCCCAGGGAGTGCTCGAGGCGCATC
  AGGCCCGTTTTTTACCAGTTTATATCACGGTCTTCATTTTTAAAAGTAACGCTAACTTTG
  TAGGGACGATGTCTCATGGATTAAATAATATTCTTTATGGCAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_024599 unedited
  TTTTNGGGGACCTTGATTTGTAATACGACTTTACTATAGGGCGGCCGCGATTCCCGGGAT
  ATCGTCGACCCACGCGTCCGGCCGCTCGCAGGCGCCGCACGGAGTTGCGTCCCGGGGACT
  TGGGGCCGCAGGGAGCTGTGAGTACCCAGGAAGCTGCACCGTGTGGCCTGGAGCTGTCTA
  TCTGTCCTTCCAGCCACCTGTCTGTCCAGCCACCCTTCCACAGACTGAGGCTTGACACCG
  GAGCATCTGTACAGAGCAAGGAGAAGACAAGAACATGCTCTAAAGCCCTTCACAGCAAGA
  CCCAGGAAGCCGCGGGCAAACTCAGACTCGAAGCCCTCCCGCCTCCTGCCCACAATGGCC
  TCTGCTGACAAGAATGGCGGGAGCGTGTCCTCTGTGTCCAGCAGCCGCCTGCAGAGCCGG
  AAGCCACCCAACCTCTCCATCACCATCCCGCCACCCGAGAAAGAGACCCAGGCCCCTGGC
  GAGCAGGACAGCATGCTGCCTGAGGGTTTTCAGAATAGGAGGCTAAAGAAAAGCCAGCCC
  AGGACCTGNGCTTCACACACCACCGCCTGCCCTCCCTCCTTCCTCCCCAAGAGGAAGAAC
  CCAGCCTACTTGAAGAGCGTCAGCCTCCAGGAGCCACGCAGCCGATGGCAGGAGAGTTCA
  GAGAAGCGCCCTGGCTTCCGCCGCCAGGCCTCACTGTCCCAGAGCATCCGCAAGGGCGCA
  GCCCAGTGGTTTGGAGTCAGCGGCGACTGGGAGGGGGCAGCGCAGCAGTGGCAGCGCCGC
  AGCCTGCACCACTGCAGCATGCGCTACGGCCGCCTGAAGGCCTCGTGCCAGCGTGACCTG
  GAGCTCCNCAGCCAGGAGGCACCGTCCTTCCAGGCACTGAGTCCCCAAGCCCTGCAGATG
  
• Restriction Sites: Please inquire
• ACCN: NM_024599
• Insert Size: 3596 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024599.2, NP_078875.2
• RefSeq Size: 3596 bp
• RefSeq ORF: 2571 bp
• Locus ID: 79651
• UniProt ID: Q6PJF5
• Cytogenetics: 17q25.1
• Protein Families: Protease, Transmembrane
• Gene Summary: Regulates ADAM17 protease, a sheddase of the epidermal growth factor (EGF) receptor ligands and TNF, thereby plays a role in sleep, cell survival, proliferation, migration and inflammation. Does not exhibit any protease activity on its own.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).