VASP (NM_003370) Human Untagged Clone

CAT#: SC122656

VASP (untagged)-Human vasodilator-stimulated phosphoprotein (VASP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VASP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003370 edited
  GCAAGGGGTGCGCGCTGGGGAGCGGACGCTGCATCCCCTTTCTGCTGCAGGAACCTCTCA
  TCAGACCGCCTGAGGGAAGCGGCGCCCGGAGACCCGCCCCGGCCCGGTCCACATTCTCCC
  CAGGAAGCCGGACTCTATGGGGCGGGACCCTGGGGGAGCCTGAGCCGAGCCCGGAGCCAG
  CCCCGAACCCCTGAACCTCCAGCCAGGGGCGCCCCGGGAGCAGCCAGCCCGTGGGCGAGC
  CGCCCGCCCGCCGAGCAGCCATGAGCGAGACGGTCATCTGTTCCAGCCGGGCCACTGTGA
  TGCTTTATGATGATGGCAACAAGCGATGGCTCCCTGCTGGCACGGGTCCCCAGGCCTTCA
  GCCGCGTCCAGATCTACCACAACCCCACGGCCAATTCCTTTCGCGTCGTGGGCCGGAAGA
  TGCAGCCCGACCAGCAGGTGGTCATCAACTGTGCCATCGTCCGGGGTGTCAAGTATAACC
  AGGCCACCCCCAACTTCCATCAGTGGCGCGACGCTCGCCAGGTCTGGGGCCTCAACTTCG
  GCAGCAAGGAGGATGCGGCCCAGTTTGCCGCCGGCATGGCCAGTGCCCTAGAGGCGTTGG
  AAGGAGGTGGGCCCCCTCCACCCCCAGCACTTCCCACCTGGTCGGTCCCGAACGGCCCCT
  CCCCGGAGGAGGTGGAGCAGCAGAAAAGGCAGCAGCCCGGCCCGTCGGAGCACATAGAGC
  GCCGGGTCTCCAATGCAGGAGGCCCACCTGCTCCCCCCGCTGGGGGTCCACCCCCACCAC
  CAGGACCTCCCCCTCCTCCAGGTCCCCCCCCACCCCCAGGTTTGCCCCCTTCGGGGGTCC
  CAGCTGCAGCGCACGGAGCAGGGGGAGGACCACCCCCTGCACCCCCTCTCCCGGCAGCAC
  AGGGCCCTGGTGGTGGGGGAGCTGGGGCCCCAGGCCTGGCCGCAGCTATTGCTGGAGCCA
  AACTCAGGAAAGTCAGCAAGCAGGAGGAGGCCTCAGGGGGGCCCACAGCCCCCAAAGCTG
  AGAGTGGTCGAAGCGGAGGTGGGGGACTCATGGAAGAGATGAACGCCATGCTGGCCCGGA
  GAAGGAAAGCCACGCAAGTTGGGGAGAAAACCCCCAAGGATGAATCTGCCAATCAGGAGG
  AGCCAGAGGCCAGAGTCCCGGCCCAGAGTGAATCTGTGCGGAGACCCTGGGAGAAGAACA
  GCACAACCTTGCCAAGGATGAAGTCGTCTTCTTCGGTGACCACTTCCGAGACCCAACCCT
  GCACGCCCAGCTCCAGTGATTACTCGGACCTACAGAGGGTGAAACAGGAGCTTCTGGAAG
  AGGTGAAGAAGGAATTGCAGAAAGTGAAAGAGGAAATCATTGAAGCCTTCGTCCAGGAGC
  TGAGGAAGCGGGGTTCTCCCTGACCACAGGGACCCAGAAGACCCGCTTCTCCTTTCCGCA
  CACCCGGCCTGTCACCCTGCTTTCCCTGCCTCTACTTGACTTGGAATTGGCTGAAGACTA
  CACAGGAATGCATCGTTCCCACTCCCCATCCCACTTGGAAAACTCCAAGGGGGTGTGGCT
  TCCCTGCTCACACCCACACTGGCTGCTGATTGGCTGGGGAGGCCCCCGCCCTTTTCTCCC
  TTTGGTCCTTCCCCTCTGCCATCCCCTTGGGGCCGGTCCCTCTGCTGGGGATGCACCAAT
  GAACCCCACAGGAAGGGGGAAGGAAGGAGGGAATTTCACATTCCCTTGTTCTAGATTCAC
  TTTAACGCTTAATGCCTTCAAAGTTTTGGTTTTTTTAAGAAAAAAAAATATATATATATT
  TGGGTTTTGGGGGAAAAGGGAAATTTTTTTTTCTCTTTGGTTTTGATAAAATGGGATGTG
  GGAGTTTTTAAATGCTATAGCCCTGGGCTTGCCCCATTTGGGGCAGCTATTTAAGGGGAG
  GGGATGTCTCACCGGGCTGGGGGTGAGACATCCCCCCACCCCAGGGACTCCCCTTCCCTC
  TGGCTCCTTCCCCTTTTCTATGAGGAAATAAGATGCTGTAACTTTTTGGAACCTCAGTTT
  TTTGATTTTTTATTTGGGTAGGTTTTGGGGTCCAGGCCATTTTTTTTACCCCTTGGAGGA
  AATAAGATGAGGGAGAAAGGAAAAGGGGAGGAAACTTCTCCCCTCCCACCTTCACCTTTA
  GCTTCTTGAAAATGGGCCCCTGCAGAATAAATCTGCCAGTTTTTATAAAAAAAAAAAAAA
  AAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003370 unedited
  TTCGCATTAGTAATACGACTTACTATAGGGCGGCCGCGATTCCCGGATGCAAGGGTGCGC
  GCTGGGGAGCGGACGCTGCATCCCCTTTCTGCTGCAGGAACCTCTCATCAGACCGCCTGA
  GGGAAGCGGCGCCCGGAGACCCGCCCCGGCCCGGTCCACATTCTCCCCAGGAAGCCGGAC
  TCTATGGGGCGGGACCCTGGGGGAGCCTGAGCCGAGCCCGGAGCCAGCCCCGAACCCCTG
  AACCTCCAGCCAGGGGCGCCCCGGGAGCAGCCAGCCCGTGGGCGAGCCGCCCGCCCGCCG
  AGCAGCCATGAGCGAGACGGTCATCTGTTCCAGCCGGGCCACTGTGATGCTTTATGATGA
  TGGCAACAAGCGATGGCTCCCTGCTGGCACGGGTCCCCAGGCCTTCAGCCGCGTCCAGAT
  CTACCACAACCCCACGGCCAATTCCTTTCGCGTCGTGGGCCGGAAGATGCAGCCCGACCA
  GCAGGTGGTCATCAACTGTGCCATCGTCCGGGGTGTCAAGTATAACCAGGCCACCCCCAA
  CTTCCATCAGTGGCGCGACGCTCGCCAGGTCTGGGGCCTCAACTTCGGCAGCAAGGAGGA
  TGCGGCCCATTTTGCCGCCGGCATGGCCAGTGCCCTATAGGCGTTGGAAGGATGTGGGCC
  CCCTCCACCCCCAGCACTTCCCACCTGGTCGGTCCCGAACGGCCCCTCCCCGGANGATGT
  GGAGCAGCAGAAAGGCAGCAGCCCGGCCCGTCNGAGCACATAGAGCGCCGGGGTCTCAAT
  GCTATGAGCCCACCTGCTCCCCCCGCTGGGGGTCCACCCCCACCACCAGTACCTCCCCCT
  TCTCCAAGTTCCCCCCCACCCCCAGTTTGCCCCCTTCGGGGTTCCAACTGCAGCGCACGG
  GCAGGGGGAGGACCACCCCTGGCCCCCCTT
  
• Restriction Sites: Please inquire
• ACCN: NM_003370
• Insert Size: 2231 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003370.3, NP_003361.1
• RefSeq Size: 2298 bp
• RefSeq ORF: 1143 bp
• Locus ID: 7408
• UniProt ID: P50552
• Cytogenetics: 19q13.32
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration
• Gene Summary: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq, Jul 2008]