CREB1 (NM_134442) Human Untagged Clone

CAT#: SC120496

CREB1 (untagged)-Human cAMP responsive element binding protein 1 (CREB1), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CREB1
• Synonyms: CREB; CREB-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120496 sequence for NM_134442 edited (data generated by NextGen Sequencing)
  
  
  ATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCAGCTGTAACAGAAGCTGAAAACCAAC
  AAATGACAGTTCAAGCCCAGCCACAGATTGCCACATTAGCCCAGGTATCTATGCCAGCAGCTCATGCAAC
  ATCATCTGCTCCCACCGTAACTCTAGTACAGCTGCCCAATGGGCAGACAGTTCAAGTCCATGGAGTCATT
  CAGGCGGCCCAGCCATCAGTTATTCAGTCTCCACAAGTCCAAACAGTTCAGTCTTCCTGTAAGGACTTAA
  AAAGACTTTTCTCCGGAACACAGATTTCAACTATTGCAGAAAGTGAAGATTCACAGGAGTCAGTGGATAG
  TGTAACTGATTCCCAAAAGCGAAGGGAAATTCTTTCAAGGAGGCCTTCCTACAGGAAAATTTTGAATGAC
  TTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAGAAGTCTGAAGAGGAGACTTCAGCACCTG
  CCATCACCACTGTAACGGTGCCAACTCCAATTTACCAAACTAGCAGTGGACAGTATATTGCCATTACCCA
  GGGAGGAGCAATACAGCTGGCTAACAATGGTACCGATGGGGTACAGGGCCTGCAAACATTAACCATGACC
  AATGCAGCAGCCACTCAGCCGGGTACTACCATTCTACAGTATGCACAGACCACTGATGGACAGCAGATCT
  TAGTGCCCAGCAACCAAGTTGTTGTTCAAGCTGCCTCTGGAGACGTACAAACATACCAGATTCGCACAGC
  ACCCACTAGCACTATTGCCCCTGGAGTTGTTATGGCATCCTCCCCAGCACTTCCTACACAGCCTGCTGAA
  GAAGCAGCACGAAAGAGAGAGGTCCGTCTAATGAAGAACAGGGAAGCAGCTCGAGAGTGTCGTAGAAAGA
  AGAAAGAATATGTGAAATGTTTAGAAAACAGAGTGGCAGTGCTTGAAAATCAAAACAAGACATTGATTGA
  GGAGCTAAAAGCACTTAAGGACCTTTACTGCCACAAATCAGATTAA
  
  Clone variation with respect to NM_134442.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_134442 unedited
  ATTTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGGCTGGCTCCCT
  GGCTGCGGCTCCTCAGTCGGCGGCGGCTGCTGCTGCCTGTGGCCCGGGCGGCTGGGAGAA
  GCGGAGTGTTGGTGAGTGACGCGGCGGAGGTGTAGTTTGACGCGGTGTGTTACGTGGGGG
  AGAGAATAAAACTCCAGCGAGATCCGGGCCGTGAACGAAAGCAGTGACGGAGGAGCTTGT
  ACCACCGGTAACTAAATGACCATGGAATCTGGAGCCGAGAACCAGCAGAGTGGAGATGCA
  GCTGTAACAGAAGCTGAAAACCAACAAATGACAGTTCAAGCCCAGCCACAGATTGCCACA
  TTAGCCCAGGTATCTATGCCAGCAGCTCATGCAACATCATCTGCTCCCACCGTAACTCTA
  GTACAGCTGCCCAATGGGCAGACAGTTCAAGTCCATGGAGTCATTCAGGCGGCCCAGCCA
  TCAGTTATTCAGTCTCCACAAGTCCAAACAGTTCAGTCTTCCTGTAAGGACTTAAAAAGA
  CTTTTCTCCGGAACACAGATTTCAACTATTGCAGAAAGTGAAGATTCACAGGAGTCAGTG
  GATAGTGTAACTGATTCCCAAAAGCGAAGGGAAATTCTTTCAGGAGGCCTTCCTACAGAN
  AATTTTGAATGACTTATCTTCTGATGCACCAGGAGTGCCAAGGATTGAAGAAGAGAAGTC
  TGAAGAGGAGACTTCAGCACCTGCCATCACCACTGTAACGGTGCCNACTCCAATTTACCA
  AACTAGCAGTGGACAGTATATTGCCATTACCCAGGGGAGAGGCATACAGCTGGCTAACAT
  GGTACCGATGGGGTACAGGGGCCTGCAACATTAACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_134442 unedited
  CGGCCGCTTTCTANATCGAGTTTTTTTTTTTTTTTTTTGAGTAACTGTTAGATTTATCGA
  GCTTAGTGAATAAAATGAGTAACAATTAAAAGTGGTATTGAAAACAATGTATGATATACA
  CAAATACAGACTGTTTTAATCAGGAATTAAAATTATAAAATGACAAAGGCTATACAGCAT
  ATTGGAAAGCTAAAGCAGAACTAATAACTACTCTTTTTACAAGAGAACAAAAAAATGTTT
  TGTAAGAATGACAATCCAAATATTGCAGTACAGCAGTCATTCAAATATGTCTGAAAATAA
  CTTTCCTTGTGCACATCTAACACTGTACTTTTTATATCCTTACAACTTCTTTCATTTAAA
  AAATTCACTAGAGATTCTCTGTTAAATTGTTAATATGGAAAATATTTAAAATCAGTATTT
  TTCTACATGTAACATTTCATAAACAAATATATATCACTTTGCTTTATTTGACATCTTGAA
  GGCTTTCTTGGTGGTGGTATGTAAGTGCAATGGGCAACAGTAAATTAGAAATACCTTTTT
  ACGTAAATGAGTGACAATATAAATATTTTCGAAGGTGAGGACAATAACATTCATTTAAAA
  ATCTTGTTATATAACTCAAGGGATTGGTTTACTGATCAGCATTCTACTAAGAATTTACCA
  CTAACTAAAACACGCTCTGTCCTTTTCTTTACTCTGCTCTAACTAAAAACTCAAATACCA
  CGCTTTGCGGCTCAGTCGCCACTTCCCTTCATGTATCGCAAACGGTGGACCCCTTCCCAA
  CTGGATTACGCACCACCCACTGCCCTCCGGAAAGAGCGGTTCACCCCATACTGGCTTTTA
  CCACCACGGCACATCCTCTCCGTTTGAATACAACACCTTCACTTCACGCCCCTGACCCCC
  TCTCCACTTCCCCCTACCCGCCAAACTTCTTCCCCATGTTCCGCCCCTTAACCCCCATAT
  TGCTACCGCCAGCCTC
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_134442
• Insert Size: 3180 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134442.2, NP_604391.1
• RefSeq Size: 10221 bp
• RefSeq ORF: 1026 bp
• Locus ID: 1385
• UniProt ID: P16220
• Cytogenetics: 2q33.3
• Domains: pKID, BRLZ
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Antigen processing and presentation, Huntington's disease, Melanogenesis, Prostate cancer
• Gene Summary: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
  Transcript Variant: This variant (2, also known as B) represents the longest transcript and encodes the longest isoform (B). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.