RFX3 (NM_134428) Human Untagged Clone

CAT#: SC120494

RFX3 (untagged)-Human regulatory factor X, 3 (influences HLA class II expression) (RFX3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFX3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_134428, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGACATCAGAGACTGGGTCGGACACAGGCTCGACAGTGACCTTACAAACATCTGTGGCTAGTCAAG
  CAGCAGTGCCTACGCAGGTGGTACAGCAAGTACCAGTACAACAACAGGTACAGCAGGTACAGACTGTGCA
  GCAGGTACAACATGTCTATCCCGCTCAGGTGCAGTATGTGGAAGGAAGCGATACTGTCTATACCAATGGA
  GCAATCCGAACAACAACGTATCCTTACACAGAGACACAGATGTACAGCCAAAATACTGGAGGGAATTACT
  TTGATACTCAAGGGAGTTCCGCCCAGGTGACTACCGTGGTCTCATCCCACAGTATGGTGGGCACTGGTGG
  GATTCAGATGGGCGTCACAGGAGGACAACTCATCAGCAGCTCTGGAGGAACCTATCTGATCGGCAACTCA
  ATGGAGAATTCTGGTCACTCAGTGACACACACAACTCGGGCCTCCCCAGCGACAATTGAAATGGCGATTG
  AGACGCTGCAAAAGTCTGACGGTCTGTCCACTCACAGAAGCTCTCTTCTCAACAGCCATCTCCAGTGGCT
  GTTGGACAATTATGAGACAGCAGAAGGAGTGAGCCTTCCCAGAAGCACTCTGTACAACCACTACCTTCGA
  CACTGTCAGGAACACAAACTGGACCCAGTCAATGCTGCCTCTTTTGGAAAATTAATAAGATCAATTTTTA
  TGGGGCTACGAACCAGGAGATTGGGCACTAGAGGAAACTCCAAATACCACTACTATGGGATTCGTGTCAA
  GCCAGATTCCCCTCTTAATCGTCTGCAAGAAGACATGCAGTATATGGCTATGAGACAACAACCCATGCAA
  CAGAAACAAAGGTACAAGCCTATGCAGAAAGTGGATGGGGTTGCAGATGGTTTCACAGGAAGTGGTCAAC
  AGACAGGCACATCTGTTGAGCAAACTGTAATTGCCCAAAGCCAACATCATCAACAGTTTTTAGATGCATC
  TCGAGCACTTCCAGAGTTTGGAGAAGTTGAAATCTCTTCTCTGCCAGATGGTACTACCTTTGAGGATATC
  AAGTCACTGCAGAGTCTTTATAGAGAGCACTGTGAGGCAATATTGGACGTTGTTGTGAATCTTCAATTTA
  GCCTGATAGAAAAATTGTGGCAAACATTCTGGCGCTATTCTCCCTCTACTCCAACTGATGGCACTACCAT
  TACCGAATCGAGCAATCTGAGTGAAATAGAAAGTCGACTTCCGAAAGCAAAGCTGATAACTCTGTGCAAA
  CATGAGTCTATCCTGAAATGGATGTGTAACTGTGACCATGGGATGTACCAGGCTTTGGTGGAGATTCTCA
  TCCCCGACGTCCTTAGACCTATTCCTAGTGCCTTGACCCAAGCCATTCGAAATTTTGCAAAAAGCCTTGA
  AGGTTGGCTTTCCAATGCCATGAACAATATTCCACAGAGAATGATACAAACCAAGGTTGCCGCTGTAAGT
  GCCTTTGCCCAGACTCTGCGAAGATACACGTCGCTTAATCACCTGGCCCAGGCAGCTCGTGCAGTGCTTC
  AGAACACTTCCCAAATCAACCAGATGCTTAGTGACCTCAACCGTGTCGACTTTGCCAATGTCCAGGAGCA
  GGCTTCCTGGGTGTGCCAGTGTGATGACAACATGGTTCAGAGACTAGAAACAGACTTCAAGATGACTCTT
  CAGCAGCAGAGCACCCTGGAGCAGTGGGCTGCGTGGCTTGACAATGTGATGATGCAAGCACTGAAACCCT
  ATGAAGGAAGACCCAGTTTTCCTAAAGCCGCCAGGCAGTTTCTGCTAAAATGGTCTTTCTACAGCTCAAT
  GGTTATTCGGGACTTAACCTTACGCAGTGCTGCTAGCTTTGGCTCCTTCCACCTGATCCGTCTACTCTAC
  GACGAATATATGTTTTACTTAGTAGAACATCGTGTTGCTCAGGCAACAGGAGAGACTCCTATAGCAGTCA
  TGGGCGAGTTTGGTGATTTAAATGCCGTGTCTCCTGGAAATCTGGATAAAGATGAAGGCAGTGAAGTAGA
  AAGTGAAATGGATGAAGAACTGGATGACTCTTCAGAGCCTCAAGCCAAAAGAGAGAAAACAGAGCTGAGC
  CAGGCATTTCCAGTGGGCTGCATGCAGCCTGTTCTCGAGACTGGCGTGCAACCAAGCCTCCTGAATCCAA
  TTCACAGCGAGCACATTGTCACAAGTACTCAGACTATCAGACAGTGCAGCGCTACAGGAAATACCTACAC
  TGCAGTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_134428 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATCAAGCGCCATAGTCACCG
  TAGTCCTGGCGACTGTTACCCATCAACAACAACTACTCCTCTCCTCCTCCTCCTCCTCTT
  CCTCCTCCTCCTCCCCACCACCACCATCTCCATCACCCACCAACAACACCACAATAATCC
  ACAGCCAAGAGACCATCATGCAGACATCAGAGACTGGGTCGGACACAGGCTCGACAGTGA
  CCTTACAAACATCTGTGGCTAGTCAAGCAGCAGTGCCTACGCAGGTGGTACAGCAAGTAC
  CAGTACAACAACAGGTACAGCAGGTACAGACTGTGCAGCAGGTACAACATGTCTATCCCG
  CTCAGGTGCAGTATGTGGAAGGAAGCGATACTGTCTATACCAATGGAGCAATCCGAACAA
  CAACGTATCCTTACACAGAGACACAGATGTACAGCCAAAATACTGGAGGGAATTACTTTG
  ATACTCAAGGGAGTTCCGCCCAGGTGACTACCGTGGTCTCATCCCACAGTATGGTGGGCA
  CTGGTGGGATTCAGATGGGCGTCACAGGAGGACAACTCATCAGCAGCTCTGGAGGAACCT
  ATCTGATCGGCAACTCAATGGAGAATTCTGGTCACTCAGTGACACACACAACTCGGGCCT
  CCCCAGCGACAATTGAAATGGCGATTGAGACGCTGCANAAGTCTGACGGTCTGTCCACTC
  ACAGAAGCTCTCTTCTCAACAGCCATCTCCAGTGGGCTGTTGGACAANTATGAGACAGCA
  GAAGGAGTGAGCCTTCCCAGAGCACTCTGTACACCACTACCTTCGACACTGTCAGGACAC
  AAACTGGACCCCAGTCATGCTGCCTNNCTTTGGNAAATNATAAGATCAATTTTTATGGGG
  CTACGACCCAGNAGATGGGGCCTAGAGGAAACTCAATACACTACTATTGGGATCGGGTCA
  GCCAGATCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_134428 unedited
  NGGACTCTCTGNNACCGCGGCCGCAATCTANNATCGAGTTTTTTTTTTTTTTTTTTTTTT
  TTTTTAAATGTAAATTACTTTTTAATTATAAGAAAACAAAACATTGACATTACTTGTTGT
  AAAAATCCTTCTTGACACCTGAAACTAAGGGAAAAAATTCATTATTAAGAAGCAAATAAT
  TTGTTTACGTTAAAAAAAAAGATCTGGCAAAATACATACACCCATTCCATTTCACAACTC
  CAAAAAGTTAATGTTCAGCACAGATAGAATTTGACAACAGTCGACCTTCAGGCTTATTAA
  ATTTTCAACTTAAGCCCAATATCAACAGGGTTAATGTAAGCTGGAAAAATACGCTTTAAT
  ATTCTTTAGACTGCAGGGTAGGTATTTCCTGTAGCGCTGCACTGTCTGATAGTCTGAGTA
  CTTGTGACAATGTGCTCGCTGTGAATTGGATTCAGGAGGCTTGGTTGCACGCCAGTCTCG
  AGAACAGGCTGCATGCAGCCCACTGGAAATGCCCTGGCTCAGCTCTGTTTTCTCTCTTTT
  GGCTTGAGGCTCTGAAGAGTCATCCAGTTCTTCATCCATTTCACTTTCTACTTCACTGCC
  TTTATCTTTATCCAGATTTCCAGGAGACCCGGCATTTACATCACCAAACTCGCCCATGAC
  TGCTATAGGAGTTTCTCCTGTTGCCTGAGCAACACGATGTTCTACTAAGTAAAACATATA
  TTCCCCCGTAAAGAAACGGATCCGGGGGGAAGGACCCAAGCTTCCACCCCTGGGAAAGGT
  AATCCCCCAAAACCATTGGGCCGGATACGAACCTTTAACAAAAACTGCCTGGCGGCTTTA
  AGAAAAACGGGTCTCCCCTTTTAGGGTTTCAGGGGCTTGCCTCTCCCTTGTCAAGCCCCC
  CCACCCATGGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_134428
• Insert Size: 2720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134428.1, NP_602304.1
• RefSeq Size: 3018 bp
• RefSeq ORF: 2250 bp
• Locus ID: 5991
• UniProt ID: P48380
• Cytogenetics: 9p24.2
• Domains: RFX_DNA_binding, RFX1_trans_act
• Protein Families: Transcription Factors
• Gene Summary: This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X2, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (b). Both variants 2 and 3 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.