PRPK (TP53RK) (NM_033550) Human Untagged Clone

CAT#: SC120376

TP53RK (untagged)-Human TP53 regulating kinase (TP53RK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRPK
• Synonyms: BUD32; C20orf64; dJ101A2; GAMOS4; Nori-2; Nori-2p; PRPK; TPRKB
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_033550 edited
  ATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGGAGGCTGAG
  GCTCTGGCCGCAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGGTGAAG
  CAGGGTGCCGAGGCGCGCGTGTTCCGTGGCCGCTTCCAGGGCCGCGCGGCGGTGATCAAG
  CACCGCTTCCCCAAGGGCTACCGGCACCCGGCGCTGGAGGCGCGGCTTGGCAGACGGCGG
  ACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCGCGCTGGAATATCTGCCCCAGTT
  GTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATTGAAGGCTCAGTG
  ACTGTTCGAGATTATATTCAGTCCACTATGGAGACTGAAAAAACTCCCCAGGGTCTCTCC
  AACTTAGCCAAGACAATTGGGCAGGTTTTGGCTCGAATGCACGATGAAGACCTCATTCAT
  GGTGATCTCACCACCTCCAACATGCTCCTGAAACCCCCCCTGGAACAGCTGAACATTGTG
  CTCATAGACTTTGGGCTGAGTTTCATTTCAGCACTTCCAGAGGATAAGGGAGTAGACCTC
  TATGTCCTGGAGAAGGCCTTCCTCAGTACCCATCCCAACACTGAAACTGTGTTTGAAGCC
  TTTCTGAAGAGCTACTCCACCTCCTCCAAAAAGGCCAGGCCAGTGCTAAAAAAATTAGAT
  GAAGTGCGCCTGAGAGGAAGAAAGAGGTCCATGGTTGGGTAG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_033550 unedited
  CTATNAGGNCAGGCCGCGCAATTCGGCACGAGGCTGATCGGTTGGAGCTGTTGCGCCGAG
  CAGTCATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGGAGG
  CTGAGGCTCTGGCCGCAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGG
  TGAAGCAGGGTGCCGAGGCGCGCGTGTTCCGTGGCCGCTTCCAGGGCCGCGCGGCGGTGA
  TCAAGCACCGCTTCCCCAAGGGCTACCGGCACCCGGCGCTGGAGGCGCGGCTTGGCAGAC
  GGCGGACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCGCGCTGGAATATCTGCCC
  CAGTTGTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATTGAAGGCT
  CAGTGACTGTTCGAGATTATATTCAGTCCACTATGGAGACTGAAAAAACTCCCCAGGGTC
  TCTCCAACTTAGCCAAGACAATTGGGCAGGTTTTGGCTCGAATGCACGATGAAGACCTCA
  TTCATGGTGATCTCACCACCTCCAACATGCTCCTGAAACCCCCCCTGGAACAGCTGAACA
  TTGTGCTCATAGACTTTGGGCTGAGTTTCATTTCAGCACTTCCAGAGGATAAGGGAGTAG
  ACCTCTATGTCCTGGAGAAGGCCTTCCTCAGTACCCATCCCAACACTGAAACTGTGTTTG
  AAGCCTTTCTGAAGAGCTACTCCACCTCCTCCAAAAAGCCAGGCCAGTGCTAAAAAAATA
  GATGAAGTGCGCCTGANAGGAAGAAAGAGGTCATGGTTGGGTAGAAGATGTGTATGACAC
  CCCACACAGTGAAGCTCTTTTTTCAAAGTAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_033550 unedited
  NTTTTAGCTCTGNACCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAT
  TTATAAAGGAAAAGTTTAATAACTCACAGTTCAGCATGGCTGGGGATGCCTCAGAAAACT
  TATAATCATGGCAGATGGGGAAACAAACACACCCTTCTCCACGTGGCAGCAGGAAGTGCC
  GAACAAGAGGGAAAAGCCCCTTATAAAACCATCAGATCTCGTGAGAACTCACTCGCTATC
  ACGAGAACAGCACGGAGGTAGCCGCCCCGAGGATTAAATTACCTCCCACTGGGTCCCTCC
  CATGTCACGTGGGGATTACGGGAACAATTCAAGATGAGATTTGGATGGGGACACAGCCAA
  ACCCTATCAACTAGGGATTTGTTGACTTAATGCACACGAGCCAACCATACCCCAACAAAG
  CAGGTACTAGCCTTATAGTACACGTGAGGAAACTGAGGAATGAGGCATCTCATTCATGTT
  GGGAATATTAATTGAAAAACCTGTCAGCTCCTGTGGCCCTTGGAGTTCCTTGAAAATAAT
  GTTATACGCCAATACTTATTATAGTACTGTAAAGATGCACCATATCCCATATCTCATTTG
  TCAGTGGAAAGAACACAGATTTGGAGCCAGGGTAAAGTACCTCTTGCTACCTGTGACGTC
  TTGGACAAGTTCCCACCCTTTTGGGGCAAGTTCCTCCATCTATAAAATGGGGATAATGCC
  TAACTTAACGAGTATTTTAGATCCCATATAAAAATTCCCAGCCCAATGCCTGGCATGCAG
  NTAGCCAGCCATTATACTATTTTCCCTCTCTACTAGCCCAACTACCCAGCAAGGGAGTTT
  TCACTCATTCCTGCCATCCGCCCATGCTGGGCACTTGGGGCTGCTGACTGGGGTGAAGAA
  CAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_033550
• Insert Size: 3200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: pass
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033550.3, NP_291028.3
• RefSeq Size: 3384 bp
• RefSeq ORF: 762 bp
• Locus ID: 112858
• UniProt ID: Q96S44
• Cytogenetics: 20q13.12
• Domains: S_TKc, KOW
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806).[UniProtKB/Swiss-Prot Function]