PRPK (TP53RK) (NM_033550) Human Tagged ORF Clone

CAT#: RC209748

TP53RK (Myc-DDK-tagged)-Human TP53 regulating kinase (TP53RK)

Product Images

Circular map for RC209748

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TP53RK (Cat# RC209748, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53RK (Cat# TA808232)(1:2000). Positive lysates LY403262 (100ug) and LC403262 (20ug) can be purchased separately from OriGene.

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  Western blot validation of overexpression lysate (Cat# LY403262) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209748 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRPK
• Synonyms: BUD32; C20orf64; dJ101A2; GAMOS4; Nori-2; Nori-2p; PRPK; TPRKB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC209748 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGGAGGCTGAGGCTCTGGCCG
  CAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGGTGAAGCAGGGTGCCGAGGCGCGCGT
  GTTCCGTGGCCACTTCCAGGGCCGCGCGGCGGTGATCAAGCACCGCTTCCCCAAGGGCTACCGGCACCCG
  GCGCTGGAGGCGCGGCTTGGCAGACGGCGGACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCGCG
  CTGGAATATCTGCCCCAGTTGTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATTGA
  AGGCTCAGTGACTGTTCGAGATTATATTCAGTCCACTATGGAGACTGAAAAAACTCCCCAGGGTCTCTCC
  AACTTAGCCAAGACAATTGGGCAGGTTTTGGCTCGAATGCACGATGAAGACCTCATTCATGGTGATCTCA
  CCACCTCCAACATGCTCCTGAAACCCCCCCTGGAACAGCTGAACATTGTGCTCATAGACTTTGGGCTGAG
  TTTCATTTCAGCACTTCCAGAGGATAAGGGAGTAGACCTCTATGTCCTGGAGAAGGCCTTCCTCAGTACC
  CATCCCAACACTGAAACTGTGTTTGAAGCCTTTCTGAAGAGCTACTCCACCTCCTCCAAAAAGGCCAGGC
  CAGTGCTAAAAAAATTAGATGAAGTGCGCCTGAGAGGAAGAAAGAGGTCCATGGTTGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC209748 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAARATTPADGEEPAPEAEALAAARERSSRFLSGLELVKQGAEARVFRGHFQGRAAVIKHRFPKGYRHP
  ALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLS
  NLAKTIGQVLARMHDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLST
  HPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033550
• ORF Size: 759 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033550.4
• RefSeq Size: 3384 bp
• RefSeq ORF: 762 bp
• Locus ID: 112858
• UniProt ID: Q96S44
• Cytogenetics: 20q13.12
• Domains: S_TKc, KOW
• Protein Families: Druggable Genome, Protein Kinase
• MW: 28.1 kDa
• Gene Summary: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806).[UniProtKB/Swiss-Prot Function]