PRPK (TP53RK) (NM_033550) Human Tagged ORF Clone

CAT#: RC209748

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TP53RK (Myc-DDK-tagged)-Human TP53 regulating kinase (TP53RK)



  "NM_033550" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 118.00

USD 429.00


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Size
    • 10 ug


Product images

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TP53RK
Synonyms BUD32; C20orf64; dJ101A2; GAMOS4; Nori-2; Nori-2p; PRPK; TPRKB
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC209748 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGGCCAGAGCTACTACGCCGGCCGATGGCGAGGAGCCCGCCCCGGAGGCTGAGGCTCTGGCCG
CAGCCCGGGAGCGGAGCAGCCGCTTCTTGAGCGGCCTGGAGCTGGTGAAGCAGGGTGCCGAGGCGCGCGT
GTTCCGTGGCCACTTCCAGGGCCGCGCGGCGGTGATCAAGCACCGCTTCCCCAAGGGCTACCGGCACCCG
GCGCTGGAGGCGCGGCTTGGCAGACGGCGGACGGTGCAGGAGGCCCGGGCGCTCCTCCGCTGTCGCCGCG
CTGGAATATCTGCCCCAGTTGTCTTTTTTGTGGACTATGCTTCCAACTGCTTATATATGGAAGAAATTGA
AGGCTCAGTGACTGTTCGAGATTATATTCAGTCCACTATGGAGACTGAAAAAACTCCCCAGGGTCTCTCC
AACTTAGCCAAGACAATTGGGCAGGTTTTGGCTCGAATGCACGATGAAGACCTCATTCATGGTGATCTCA
CCACCTCCAACATGCTCCTGAAACCCCCCCTGGAACAGCTGAACATTGTGCTCATAGACTTTGGGCTGAG
TTTCATTTCAGCACTTCCAGAGGATAAGGGAGTAGACCTCTATGTCCTGGAGAAGGCCTTCCTCAGTACC
CATCCCAACACTGAAACTGTGTTTGAAGCCTTTCTGAAGAGCTACTCCACCTCCTCCAAAAAGGCCAGGC
CAGTGCTAAAAAAATTAGATGAAGTGCGCCTGAGAGGAAGAAAGAGGTCCATGGTTGGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC209748 protein sequence
Red=Cloning site Green=Tags(s)

MAAARATTPADGEEPAPEAEALAAARERSSRFLSGLELVKQGAEARVFRGHFQGRAAVIKHRFPKGYRHP
ALEARLGRRRTVQEARALLRCRRAGISAPVVFFVDYASNCLYMEEIEGSVTVRDYIQSTMETEKTPQGLS
NLAKTIGQVLARMHDEDLIHGDLTTSNMLLKPPLEQLNIVLIDFGLSFISALPEDKGVDLYVLEKAFLST
HPNTETVFEAFLKSYSTSSKKARPVLKKLDEVRLRGRKRSMVG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_033550
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033550.4
RefSeq Size 3384 bp
RefSeq ORF 762 bp
Locus ID 112858
UniProt ID Q96S44
Cytogenetics 20q13.12
Domains S_TKc, KOW
Protein Families Druggable Genome, Protein Kinase
MW 28.1 kDa
Gene Summary Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806).[UniProtKB/Swiss-Prot Function]

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