Lysosomal acid lipase (LIPA) (NM_000235) Human Untagged Clone

CAT#: SC120024

LIPA (untagged)-Human lipase A, lysosomal acid, cholesterol esterase (LIPA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Lysosomal acid lipase
• Synonyms: CESD; LAL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120024 sequence for NM_000235 edited (data generated by NextGen Sequencing)
  ATGAAAATGCGGTTCTTGGGGTTGGTGGTCTGTTTGGTTCTCTGGACCCTGCATTCTGAG
  GGGTCTAGAGGGAAACTGACAGCTGTGGATCCTGAAACAAACATGAATGTGAGTGAAATT
  ATCTCTTACTGGGGATTCCCTAGTGAGGAATACCTAGTTGAGACAGAAGATGGATATATT
  CTGTGCCTTAACCGAATTCCTCATGGGAGGAAGAACCATTCTGACAAAGGTCCCAAACCA
  GTTGTCTTCCTGCAACATGGCTTGCTGGCAGATTCTAGTAACTGGGTCACAAACCTTGCC
  AACAGCAGCCTGGGCTTCATTCTTGCTGATGCTGGTTTTGACGTGTGGATGGGCAACAGC
  AGAGGAAATACCTGGTCTCGGAAACATAAGACACTCTCAGTTTCTCAGGATGAATTCTGG
  GCTTTCAGTTATGATGAGATGGCAAAATATGACCTACCAGCTTCCATTAACTTCATTCTG
  AATAAAACTGGCCAAGAACAAGTGTATTATGTGGGTCATTCTCAAGGCACCACTATAGGT
  TTTATAGCATTTTCACAGATCCCTGAGCTGGCTAAAAGGATTAAAATGTTTTTTGCCCTG
  GGTCCTGTGGCTTCCGTCGCCTTCTGTACTAGCCCTATGGCCAAATTAGGACGATTACCA
  GATCATCTCATTAAGGACTTATTTGGAGACAAAGAATTTCTTCCCCAGAGTGCGTTTTTG
  AAGTGGCTGGGTACCCACGTTTGCACTCATGTCATACTGAAGGAGCTCTGTGGAAATCTC
  TGTTTTCTTCTGTGTGGATTTAATGAGAGAAATTTAAATATGTCTAGAGTGGATGTATAT
  ACAACACATTCTCCTGCTGGAACTTCTGTGCAAAACATGTTACACTGGAGCCAGGCTGTT
  AAATTCCAAAAGTTTCAAGCCTTTGACTGGGGAAGCAGTGCCAAGAATTATTTTCATTAC
  AACCAGAGTTATCCTCCCACATACAATGTGAAGGACATGCTTGTGCCGACTGCAGTCTGG
  AGCGGGGGTCACGACTGGCTTGCAGATGTCTACGACGTCAATATCTTACTGACTCAGATC
  ACCAACTTGGTGTTCCATGAGAGCATTCCGGAATGGGAGCATCTTGACTTCATTTGGGGC
  CTGGATGCCCCTTGGAGGCTTTATAATAAAATTATTAATCTAATGAGGAAATATCAGTGA
  
  Clone variation with respect to NM_000235.2
  67 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000235 unedited
  CTTTTGTATACGACTAACTATAGGCGGCCGCGATTCGCCGAGCGCGGCCCGGCAGACACT
  CCGATGAAAAGCGGTTCTTGGGGTTGGTGGTCTGTTTGGTTCTCTGGACCCTGCATTCTG
  AGGGGTCTAGAGGGAAACTGACAGCTGTGGATCCTGAAACAAACATGAATGTGAGTGAAA
  TTATCTCTTACTGGGGATTCCCTAGTGAGGACATACCTAGTTGAGACAGAAGATGGATAT
  ATTCTGTGCCTTAACCGAATTCCTCATGGGAGGAAGAACCATTCTGACAAAGGTCCCAAA
  CCAGTTGTCTTCCTGCAACATGGCTTGCTGGCAGATTCTAGTAACTGGGTCACAAACCTT
  GCCAACAGCAGCCTGGGCTTCATTCTTGCTGATGCTGGTTTTGACGTGTGGATGGGCAAC
  AGCAGAGGAAATACCTGGTCTCGGAAACATAAGACACTCTCAGTTTCTCAGGATGAATTC
  TGGGCTTTCAGTTATGATGAGATGGCAAAATATGACCTACCAGCTTCCATTAACTTCATT
  CTGAATAAAACTGGCCAAGAACAAGTGTATTATGTGGGTCATTCTCAAGGCACCACTATA
  GGTTTTATAGCATTTTCACAGATCCCTGAGCTGGCTAAAAGGATTAAAATGTTTTTTGCC
  CTGNGTCCTGTGGCTTCCGTCGCCTTCTGTACTAGCCCTATGGCCAAATTANGACGATTA
  CCAGATCATCTCATTAAGGACTTATTTGGAGACAAAGAATTTCTTCCCCAGAGTGCNGTN
  TTGAAGTGGCTGGGTACCCACGTTTGCACTCATGTCATACTGAAGGAGCTCTGTGGAAAT
  CTCTGTTTCCTCCTGTGTGGATTAATGAGAGGAATTTAATATGTCTAGAGTGATGTATAT
  ACACACATTCTCTGCTGGAACTNCTGTGCAAACATGTACACTGGAGCCAGCTGTNTAATT
  CCAAGTTTCAGCCTTTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000235 unedited
  NNTTTTTTGCTTTGGACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTG
  CCTATAAGTCTCACTCAATTTTATTGAAAAGTTTAAGACTTTAAAAGTTACAAAGTAGTA
  TTCGCCCTGCCTATATTAACCATTAACAAGCATTGTACTGTTAGTGCAGCTAAAGTAATT
  ATATCAGAAAAACATATCAATTGTCATTTTGTCCACCGGACAGTATTGTAAGGAAATGAT
  GCTGCAAGAAAAGCAGACAAATATGGACTTCCCAACATAGGGACGTGAAGCAGGTGTATT
  AAAAATAAGGATGCATATAAAACTAAGACACAAAATGAATCCCTGAGCTGAGTTTGCATC
  CGAAAACATCACGTTCACACTTCATTTGGGTGAAAATGAACAAGGACACTTCTCATGGGC
  AACCACGGGGCTTTGGCTTCACACAGATGTGATCAATAACGACAGTAGCGGGCTCCAAGA
  GTACATTAATCTCAGAAAAAATAGTGGTATAGTCTCCACAGGGATTTGCTTCTCAGATAA
  GTAGAACTATGGCCTGCCATGAAATTGAGAGCTCATGAATATAGATGGTTGAGTGTATCA
  GTCTTCAAAGCATTTAAAACATACTTTTGTTTTTGTTTTATCAGTGCAATTTGTTTTTGA
  AGACGCCGGANAACTATTTCATTTAACCTGAGCAATTAAAACGTGTCAAATCACAGCTGT
  CACAGTTAGGTTTGATAAAATTAATTGTTAGCATCCTTATTACTGGCACAGCTGTGATAT
  TTCTGGATTTGACTATGCACCTGCTGCNAGAAACATGATAAATGTCATTTAGATCTCTAC
  TACAAGACCGTTAGACTAGAGAGCTCTTTAAGGCAGGACATGAAACTGCGTNACTTTATA
  CTCGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000235
• Insert Size: 2550 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000235.2, NP_000226.2
• RefSeq Size: 2654 bp
• RefSeq ORF: 1200 bp
• Locus ID: 3988
• UniProt ID: P38571
• Cytogenetics: 10q23.31
• Domains: abhydrolase, abhydro_lipase
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Steroid biosynthesis
• Gene Summary: This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (2) lacks an internal segment in the 5' UTR, as compared to variant 1. Variants 1 and 2 encode the same isoform (1).