NAGLU (NM_000263) Human Untagged Clone

CAT#: SC120003

NAGLU (untagged)-Human N-acetylglucosaminidase, alpha (NAGLU)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NAGLU
• Synonyms: CMT2V; MPS-IIIB; MPS3B; NAG; UFHSD
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000263, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGCGGTGGCGGTGGCCGCGGCGGTGGGGGTCCTTCTCCTGGCCGGGGCCGGGGGCGCGGCAGGCG
  ACGAGGCCCGGGAGGCGGCGGCCGTGCGGGCGCTCGTGGCCCGGCTGCTGGGGCCAGGCCCCGCGGCCGA
  CTTCTCCGTGTCGGTGGAGCGCGCTCTGGCTGCCAAGCCGGGCTTGGACACCTACAGCCTGGGCGGCGGC
  GGCGCGGCGCGCGTGCGGGTGCGCGGCTCCACGGGCGTGGCGGCCGCCGCGGGGCTGCACCGCTACCTGC
  GCGACTTCTGTGGCTGCCACGTGGCCTGGTCCGGCTCTCAGCTGCGCCTGCCGCGGCCACTGCCAGCCGT
  GCCGGGGGAGCTGACCGAGGCCACGCCCAACAGGTACCGCTATTACCAGAATGTGTGCACGCAAAGCTAC
  TCTTTCGTGTGGTGGGACTGGGCCCGCTGGGAGCGAGAGATAGACTGGATGGCGCTGAATGGCATCAACC
  TGGCACTGGCCTGGAGCGGCCAGGAGGCCATCTGGCAGCGGGTGTACCTGGCCTTGGGCCTGACCCAGGC
  AGAGATCAATGAGTTCTTTACTGGTCCTGCCTTCCTGGCCTGGGGGCGAATGGGCAACCTGCACACCTGG
  GATGGCCCCCTGCCCCCCTCCTGGCACATCAAGCAGCTTTACCTGCAGCACCGGGTCCTGGACCAGATGC
  GCTCCTTCGGCATGACCCCAGTGCTGCCTGCATTCGCGGGGCATGTTCCCGAGGCTGTCACCAGGGTGTT
  CCCTCAGGTCAATGTCACGAAGATGGGCAGTTGGGGCCACTTTAACTGTTCCTACTCCTGCTCCTTCCTT
  CTGGCTCCGGAAGACCCCATATTCCCCATCATCGGGAGCCTCTTCCTGCGAGAGCTGATCAAAGAGTTTG
  GCACAGACCACATCTATGGGGCCGACACTTTCAATGAGATGCAGCCACCTTCCTCAGAGCCCTCCTACCT
  TGCCGCAGCCACCACTGCCGTCTATGAGGCCATGACTGCAGTGGATACTGAGGCTGTGTGGCTGCTCCAA
  GGCTGGCTCTTCCAGCACCAGCCGCAGTTCTGGGGGCCCGCCCAGATCAGGGCTGTGCTGGGAGCTGTGC
  CCCGTGGCCGCCTCCTGGTTCTGGACCTGTTTGCTGAGAGCCAGCCTGTGTATACCCGCACTGCCTCCTT
  CCAGGGCCAGCCCTTCATCTGGTGCATGCTGCACAACTTTGGGGGAAACCATGGTCTTTTTGGAGCCCTA
  GAGGCTGTGAACGGAGGCCCAGAAGCTGCCCGCCTCTTCCCCAACTCCACCATGGTAGGCACGGGCATGG
  CCCCCGAGGGCATCAGCCAGAACGAAGTGGTCTATTCCCTCATGGCTGAGCTGGGCTGGCGAAAGGACCC
  AGTGCCAGATTTGGCAGCCTGGGTGACCAGCTTTGCCGCCCGGCGGTATGGGGTCTCCCACCCGGACGCA
  GGGGCAGCGTGGAGGCTACTGCTCCGGAGTGTGTACAACTGCTCCGGGGAGGCCTGCAGGGGCCACAATC
  GTAGCCCGCTGGTCAGGCGGCCGTCCCTACAGATGAATACCAGCATCTGGTACAACCGATCTGATGTGTT
  TGAGGCCTGGCGGCTGCTGCTCACATCTGCTCCCTCCCTGGCCACCAGCCCCGCCTTCCGCTACGACCTG
  CTGGACCTCACTCGGCAGGCAGTGCAGGAGCTGGTCAGCTTGTACTATGAGGAGGCAAGAAGCGCCTACC
  TGAGCAAGGAGCTGGCCTCCCTGTTGAGGGCTGGAGGCGTCCTGGCCTATGAGCTGCTGCCGGCACTGGA
  CGAGGTGCTGGCTAGTGACAGCCGCTTCTTGCTGGGCAGCTGGCTAGAGCAGGCCCGAGCAGCGGCAGTC
  AGTGAGGCCGAGGCCGATTTCTACGAGCAGAACAGCCGCTACCAGCTGACCTTGTGGGGGCCAGAAGGCA
  ACATCCTGGACTATGCCAACAAGCAGCTGGCGGGGTTGGTGGCCAACTACTACACCCCTCGCTGGCGGCT
  TTTCCTGGAGGCGCTGGTTGACAGTGTGGCCCAGGGCATCCCTTTCCAACAGCACCAGTTTGACAAAAAT
  GTCTTCCAACTGGAGCAGGCCTTCGTTCTCAGCAAGCAGAGGTACCCCAGCCAGCCGCGAGGAGACACTG
  TGGACCTGGCCAAGAAGATCTTCCTCAAATATTACCCCCGCTGGGTGGCCGGCTCTTGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000263 unedited
  CCTGTCAGCATTTGTATACGACNACTATAGGCGGCACGCGAATTCGCACCAGCCGCAGGA
  CTGAGACCATGGAGGCGGTGGCGGTGGCCGCGGCGGTGGGGGTCCTTCTCCTGGCCGGGG
  CCGGGGGCGCGGCAGGCGACGATGCCCGGGAGGCGGCGGCCGTGCGGGCGCTCGTGGCCC
  GGCTGCTGGGGCCAGGCCCCGCGGCCGACTTCTCCGTGTCGGTGGAGCGCGCTCTGGCTG
  CCAAGCCGGGCTTGGACACCTACAGCCTGGGCGGCGGCGGCGCGGCGCGCGTGCGGGTGC
  GCGGCTCCACGGGCGTGGCGGCCGCCGCGGGGCTGCACCGCTACCTGCGCGACTTCTGTG
  GCTGCCACGTGGCCTGGTCCGGCTCTCAGCTGCGCCTGCCGCGGCCACTGCCAGCCGTGC
  CGGGGGAGCTGACCGAGGCCACGCCCAACAGGTACCGCTATTACCAGAATGTGTGCACGC
  AAAGCTACTCCTTCGTGTGGTGGGACTGGGCCCGCTGGGAGCGAGAGATAGACTGGATGG
  CGCTGAATGGCATCAACCTGGCACTGGCCTGGAGCGGCCAGGATGCCATCTGGCAGCGGG
  TGTACCTGGCCTTGGGCCTGACCCAGGCAGAGATCAATGAGTTCTTTACTGGTCCTGCCT
  TCCTGGCCTGGGGGCGAATGGGCAACCTGCACACCTGGGATGGCCCCCTGCCCCCCTCCT
  GGCACATCAAGCAGCTNTACCTGCAGCACCGGGTCCTGGACCATATGCGCTCCTTCGGCA
  TGACCCCAGTGCTGNCTGCATTCGCGGAGCATGTTCCCGATGCTGTCACCAGGGTGTTCC
  CTCAGTCAATGTCACGAAATGGGCAGTTGGGGCCACTTTACTGTNCTACNCTGCTCCTCC
  TTTGGCTCCGGATGACCCATATTCCAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000263 unedited
  TTTTGGTTAATTTCTATGNNACCGCGGCCGCATTCTAGNGATCGATTTTTTTTTTTTTTT
  TTTTAAGTGGAAAGAAAACAGTACTTTAATCCCACACTTTGGGTGGTGGTGGAGGGCAGG
  TCATTTCCCTCCAATCCCCCCAGGTCAGACCCCACCAGCAGGCCGTGGGGCCTCCTCCCA
  GGCCTGGGTTATCCTGTGATGTCTGTCCAGCTCTGGGCCCTGGAATCTGCCCTGGAATTA
  GCGGAAAACAAGGCCCAGTGGTGGCGAATCTATCACCAAGAGCCGGCCACCCAGCGGGGG
  TAATATTTGAGGAAGATCTTCTTGGCCAGGTCCACAGTGTCTCCTCGCGGCTGGCTGGGG
  TACCTCTGCTTGCTGAGAACGAAGGCCTGCTCCAGTTGGAAGACATTTTTGTCAAACTGG
  TGCTGTTGGAAAGGGATGCCCTGGGCCACACTGTCAACCAGCGCCTCCAGGAAAAGCCGC
  CAGCGAGGGGTGTAGTAGTTGGCCACCAACCCCGCCAGCTGCTTGTTGGCATAGTCCAGG
  ATGTTGCCTTCTGGCCCCCACAAGGTCAGCTGGTAGCGGCTGTTCTGCTCGTAGAAATCG
  GCCTCGGCCTCACTGACTGCCGCTGCTCGGGCCTGCTCTAGCCAGCTGCCCAACAAGAAG
  CGGCTGTTACTAGCCAGCACCTCGTCCAGTGCCGGCAGCAGCTCATAGGCCAAGACGCCT
  CCAGCCCTCACAAGGGAGGCCAGCTCCTTTGCTAAGTAGGCGCTTCTTGGCTCCCTATAA
  AACAAGCTTGACAACTCCTGCACTGGCTGGCGAATGAAGGTCCACCAGTTCTTACCGGAA
  GCGGGGGCTTGTTGCCACAGGAGGAAACAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000263
• Insert Size: 2300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000263.3, NP_000254.2
• RefSeq Size: 2798 bp
• RefSeq ORF: 2232 bp
• Locus ID: 4669
• UniProt ID: P54802
• Cytogenetics: 17q21.2
• Domains: NAGLU
• Protein Families: Druggable Genome
• Protein Pathways: Glycosaminoglycan degradation, Lysosome, Metabolic pathways
• Gene Summary: This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq, Jul 2008]