Claudin 5 (CLDN5) (NM_003277) Human Untagged Clone

CAT#: SC118095

CLDN5 (untagged)-Human claudin 5 (CLDN5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Claudin 5
• Synonyms: AWAL; BEC1; CPETRL1; TMDVCF; TMVCF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003277, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCCGCGCACGGATTGGCTGCTTCGGGCCGGGGGGCCGGGCCCGGGGGACAGAATCCGCCCCCGAAC
  CTTCAAAGAGGGTACCCCCCGGCAGGAGCTGGCAGACCCAGGAGGTGCGACAGACCCGCGGGGCAAACGG
  ACTGGGGCCAAGAGCCGGGAGCGCGGGCGCAAAGGCACCAGGGCCCGCCCAGGGCGCCGCGCAGCACGGC
  CTTGGGGGTTCTGCGGGCCTTCGGGTGCGCGTCTCGCCTCTAGCCATGGGGTCCGCAGCGTTGGAGATCC
  TGGGCCTGGTGCTGTGCCTGGTGGGCTGGGGGGGTCTGATCCTGGCGTGCGGGCTGCCCATGTGGCAGGT
  GACCGCCTTCCTGGACCACAACATCGTGACGGCGCAGACCACCTGGAAGGGGCTGTGGATGTCGTGCGTG
  GTGCAGAGCACCGGGCACATGCAGTGCAAAGTGTACGACTCGGTGCTGGCTCTGAGCACCGAGGTGCAGG
  CGGCGCGGGCGCTCACCGTGAGCGCCGTGCTGCTGGCGTTCGTTGCGCTCTTCGTGACCCTGGCGGGCGC
  GCAGTGCACCACCTGCGTGGCCCCGGGCCCGGCCAAGGCGCGTGTGGCCCTCACGGGAGGCGTGCTCTAC
  CTGTTTTGCGGGCTGCTGGCGCTCGTGCCACTCTGCTGGTTCGCCAACATTGTCGTCCGCGAGTTTTACG
  ACCCGTCTGTGCCCGTGTCGCAGAAGTACGAGCTGGGCGCAGCGCTGTACATCGGCTGGGCGGCCACCGC
  GCTGCTCATGGTAGGCGGCTGCCTCTTGTGCTGCGGCGCCTGGGTCTGCACCGGCCGTCCCGACCTCAGC
  TTCCCCGTGAAGTACTCAGCGCCGCGGCGGCCCACGGCCACCGGCGACTACGACAAGAAGAACTACGTCT
  GA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003277 unedited
  GTGGATTTTGTAATACGATTTACTATAGGGCGGCCCGCGATTCGGCACGAGGCGCGTCTC
  GCCTCTAGCCATGGGGTCCGCAGCGTTGGAGATCCTGGGCCTGGTGCTGTGCCTGGTGGG
  CTGGGGGGGTCTGATCCTGGCGTGCGGGCTGCCCATGTGGCAGGTGACCGCCTTCCTGGA
  CCACAACATCGTGACGGCGCAGACCACCTGTTAAGGGGCTGTGGATGTCGTGCGTGGTGC
  AGAGCACCGGGCACATGCAGTGCAAAGTGTACGACTCGGTGCTGGCTCTGAGCACCGAGG
  TGCAGGCGGCGCGGGCGCTCACCGTGAGCGCCGTGCTGCTGGCGTTCGTTGCGCTCTTCG
  TGACCCTGGCGGGCGCGCAGTGCACCACCTGCGTGGCCCCGGGCCCGGCCAAGGCGCGTG
  TGGCCCTCACGGGAGGCGTGCTCTACCTGTTTTGCGGGCTGCTGGCGCTCGTGCCACTCT
  GCTGGTTCGCCAACATTGTCGTCCGCGAGTTTTACGACCCGTCTGTGCCCGTGTCGCAGA
  AGTACGAGCTGGGCGCAGCGCTGTACATCGGCTGGGCGGCCACCGCGCTGCTCATGGTAG
  GCGGCTGCCTCTTGTGCTGCGGCGCCTGGGTCTGCACCGGCCGTCCCGACCTCAGCTTCC
  CCGTGAAGTACTCAGCGCCGCGGCGGCCCACGGCCACCGGCGACTACGACAAGAAGAACT
  ACGTCTGAGGGCGCTGGCACCGGCCGNGCCCCTCCTGCCAGCCACGCCTGCGAAGCCGTT
  GGATAAGCCTTGGGATCCCCGCATGAACCGCGGCTTCCGCCGGTTACCGCGCGCCCAAGC
  TCTTCGAACGTCCGGTCTGCGCCCCACGCGCTTCCGGAACCCCTCTGCCTGCCCCGCATT
  GACCTTTCCTGCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003277 unedited
  GCACTTCCAGGGCCAGGNANAGCACTGGGGAGGGGTCACAGGGTAGCCACCCGGGATCTG
  TTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTT
  GAGAGTTCAAACCATTTACTAAGCAGATTCTTAGCCTTCCCACTCCCGCCCTCTCTCAAG
  CTCCGGTGCCCACAAGCCTTGCCTGGGGAGATGCTGGAGTGAGACCGGGAGGTCCAGGCC
  AAGTCACTGGTCCCTGGGCTCGGGCCCTGCCGATGGAGTAAAGACCAGGTGTACACATCT
  TCCGGTGGGGGCCCTGGGCTCTGCATCCGCCCCTCCGAAGTCAGCAGGAGCCTCTGGGAA
  GTAAGGCAGCAGCCAAGACCCCCAGCGTCTTGGAGGGGAAGCGAAATCCTCAGTCTGACA
  CCCGCTCTGCCTATGGAAACAGCGCCGCGCACAGAAAAGGAAACTTCATTCCGTCTGTTA
  AGGGCAGGGCCGGGCTAGTGGAGGNAAAGGTCAGCTGCGGGCGCAGGCAGGAGCGGATCC
  CAGAGCCGCGTCGGGGCGCAAAGCCGGACGTTCCGAGGAGCCTGCGCGCCGCGCTACCCG
  GGGGGAGCCGCGGTCCATGCCGGGGATCCCCAGGCTTATCCAACGCCTCNCCAGCGTGGG
  CTGGAAAGAGAGGGGCCCCGCCCGGGCCCAACGCCCTTAAACGTTATTCTTCTTGGCCTA
  ATCCCCCGGGGGCCGGGGGCCGCCGCGGGCCTTAATCTTTACGGGGGAACCTGAGAGCGG
  GAAAGGCCGGGTGCAAACCAAGGCGCCAAGACAAAAAGGCAACCGCCTACATGAGACGAG
  GGGGGGGCCCGCCCACCAAAGTAAAGCCTGGGCCCCATTTGGTCTTTTGCGAACGGGCCC
  AAGAGGGTTGCAAAAATTGGCGCAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003277
• Insert Size: 1340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003277.2, NP_003268.1
• RefSeq Size: 1720 bp
• RefSeq ORF: 657 bp
• Locus ID: 7122
• UniProt ID: O00501
• Cytogenetics: 22q11.21
• Domains: PMP22_Claudin
• Protein Families: Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction
• Gene Summary: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1, and encodes a protein (isoform 1) of 303 aa. A common SNP (rs885985) encodes a stop codon in the 5' coding region and thus, for some haplotypes, translation is predicted to initiate from a downstream AUG to produce a protein of 218 aa (isoform 2).