CASK (NM_003688) Human Untagged Clone

CAT#: SC117810

CASK (untagged)-Human calcium/calmodulin-dependent serine protein kinase (MAGUK family) (CASK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASK
• Synonyms: CAGH39; CAMGUK; CMG; FGS4; hCASK; LIN2; MICPCH; MRXSNA; TNRC8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003688 edited
  ATGGCCGACGACGACGTGCTGTTCGAGGATGTGTACGAGCTGTGCGAGGTGATCGGAAAG
  GGTCCCTTCAGTGTTGTACGACGATGTATCAACAGAGAAACTGGGCAACAATTTGCTGTA
  AAAATTGTTGATGTAGCCAAGTTCACATCAAGTCCAGGGTTAAGTACAGAAGATCTAAAG
  CGGGAAGCCAGTATCTGTCATATGCTGAAACATCCACACATTGTAGAGTTATTGGAGACA
  TATAGCTCAGATGGAATGCTTTACATGGTTTTCGAATTTATGGATGGAGCAGATCTGTGT
  TTTGAAATCGTAAAGCGAGCTGACGCTGGTTTTGTGTACAGTGAAGCTGTAGCCAGCCAT
  TATATGAGACAGATACTGGAAGCTCTACGCTACTGCCATGATAATAACATAATTCACAGG
  GATGTGAAGCCCCACTGTGTTCTCCTTGCCTCAAAAGAAAACTCGGCACCTGTTAAACTT
  GGAGGCTTTGGGGTAGCTATTCAATTAGGGGAGTCTGGACTTGTAGCTGGAGGACGTGTT
  GGAACACCTCATTTTATGGCACCAGAAGTGGTCAAAAGAGAGCCTTACGGAAAGCCTGTA
  GACGTCTGGGGGTGCGGTGTGATCCTTTTTATCCTGCTCAGTGGTTGTTTGCCTTTTTAC
  GGAACCAAGGAAAGATTGTTTGAAGGCATTATTAAAGGAAAATATAAGATGAATCCAAGG
  CAGTGGAGCCATATCTCTGAAAGTGCCAAAGACCTAGTACGTCGCATGCTGATGCTGGAT
  CCAGCTGAAAGGATCACTGTTTATGAAGCACTGAATCACCCATGGCTTAAGGAGCGGGAT
  CGTTACGCCTACAAGATTCATCTTCCAGAAACAGTAGAGCAGCTGAGGAAATTCAATGCA
  AGGAGGAAACTAAAGGGTGCAGTACTAGCCGCTGTGTCAAGTCACAAATTCAACTCATTC
  TATGGGGATCCCCCTGAAGAGTTACCAGATTTCTCCGAAGACCCTACCTCCTCAGGAGCA
  GTCTCACAGGTGCTGGACAGCCTGGAAGAGATTCATGCGCTTACAGACTGCAGTGAAAAG
  GACCTAGATTTTCTACACAGTGTTTTCCAGGATCAGCATCTTCACACACTACTAGATCTG
  TATGACAAAATTAACACAAAGTCTTCACCACAAATCAGGAATCCTCCAAGCGATGCAGTA
  CAGAGAGCCAAAGAGGTATTGGAAGAAATTTCATGTTACCCTGAGAATAACGACGCAAAG
  GAACTAAAGCGTATTTTAACACAACCTCATTTCATGGCCTTACTTCAGACTCACGACGTA
  GTGGCACATGAAGTTTACAGTGATGAAGCATTGAGGGTCACACCTCCTCCCACCTCTCCC
  TATTTAAACGGCGATTCTCCAGAAAGTGCTAACGGAGACATGGATATGGAGAATGTGACC
  AGAGTTCGGCTGGTACAGTTTCAAAAGAACACAGATGAACCAATGGGAATCACTTTAAAA
  ATGAATGAACTAAATCATTGTATTGTTGCAAGAATTATGCATGGGGGCATGATTCACAGG
  CAAGGTACACTTCATGTTGGTGATGAAATTCGAGAAATCAATGGCATCAGTGTGGCTAAC
  CAAACAGTGGAACAACTGCAAAAAATGCTTAGGGAAATGCGGGGGAGTATTACCTTCAAG
  ATTGTGCCAAGTTACCGCACTCAGTCTTCGTCCTGTGAGGACTTGCCATCAACTACCCAA
  CCAAAAGGACGACAGATCTATGTAAGAGCACAATTTGAATATGATCCAGCCAAGGATGAC
  CTCATCCCCTGTAAAGAAGCTGGCATTCGATTCAGAGTTGGTGACATCATCCAGATTATT
  AGTAAGGATGATCATAATTGGTGGCAGGGTAAACTGGAAAACTCCAAAAATGGAACTGCA
  GGTCTCATTCCTTCTCCTGAACTTCAGGAATGGCGAGTAGCTTGCATTGCCATGGAGAAG
  ACCAAACAGGAGCAGCAGGCCAGCTGTACTTGGTTTGGCAAGAAAAAGAAGCAGTACAAA
  GATAAATATTTGGCAAAGCACAATGCAGTGTTTGATCAATTAGATCTTGTCACATATGAA
  GAAGTAGTAAAACTGCCAGCATTCAAGAGGAAAACACTAGTCTTATTAGGCGCACATGGT
  GTTGGGAGAAGACACATAAAAAACACTCTCATCACAAAGCACCCAGACCGGTTTGCGTAC
  CCTATTCCACATACAACCAGACCTCCAAAGAAAGACGAAGAAAATGGAAAGAATTATTAC
  TTTGTATCTCATGACCAAATGATGCAAGACATCTCTAATAACGAGTACTTGGAGTACGGC
  AGCCACGAGGATGCGATGTATGGGACAAAACTGGAGACCATCCGGAAGATCCACGAGCAG
  GGGCTGATTGCAATACTGGACGTGGAGCCTCAGGCACTGAAGGTCCTGAGAACTGCAGAG
  TTTGCTCCTTTTGTTGTTTTCATTGCTGCACCAACTATTACTCCAGGTTTAAATGAGGAT
  GAATCTCTTCAGCGTCTGCAGAAGGAGTCTGACATCTTACAGAGAACATATGCACACTAC
  TTCGATCTCACAATTATCAACAATGAAATTGATGAGACAATCAGACATCTGGAGGAAGCT
  GTTGAGCTCGTGTGCACAGCCCCACAGTGGGTCCCTGTCTCCTGGGTCTATTAGGCCTCT
  CCCCAGATATCTGAGCATAACTGGGAGCACCTCATTTGTGGAAAAGCCTCTTTGTTATCG
  GCCTTGTGTCAGCAGGTCATGGTCCCTAGAGACTACCTAGTTGTAGTGTGACCTACATTT
  ATAATTATTGTCATGTCCGAATAGATAGGAGGAGAAAAACAATTACACACTAATTTAAAG
  AGACAGTATCTTTTTTAATCAGTTCTCCTAAACTTTAATAAAATGTATCTTTAAATGTAT
  GTATTATTCAATCCTTTGGAATGTTATATTTTTGGAAATCATAGCTTTTTATTTCCAAGG
  CCCCTAAAAACTGCACAAAATAGATGCTGCTTTCTATAATCTATTTTAATAATAATAAAC
  AATGATTCTGTTACCTTGAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC
  GGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCC
  TCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003688 unedited
  ATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGTTTTCGAAGCCCTCCAC
  GCTGCGGCCGCTATCCCCTCCGGACCATGGCCGACGACGACGTGCTGTTCGAGGATGTGT
  ACGAGCTGTGCGAGGTGATCGGAAAGGGTCCCTTCAGTGTTGTACGACGATGTATCAACA
  GAGAAACTGGGCAACAATTTGCTGTAAAAATTGTTGATGTAGCCAAGTTCACATCAAGTC
  CAGGGTTAAGTACAGAAGATCTAAAGCGGGAAGCCAGTATCTGTCATATGCTGAAACATC
  CACACATTGTAGAGTTATTGGAGACATATAGCTCAGATGGAATGCTTTACATGGTTTTCG
  AATTTATGGATGGAGCAGATCTGTGTTTTGAAATCGTAAAGCGAGCTGACGCTGGTTTTG
  TGTACAGTGAAGCTGTAGCCAGCCATTATATGAGACAGATACTGGAAGCTCTACGCTACT
  GCCATGATAATAACATAATTCACAGGGATGTGAAGCCCCACTGTGTTCTCCTTGCCTCAA
  AAGAAAACTCGGCACCTGTTAAACTTGGAGGCTTTGGGGTAGCTATTCAATTAGGGGAGT
  CTGGACTTGTAGCTGGAGGACGTGTTGGAACACCTCATTNTATGGCACCAGAAGTGGTCA
  AAAGAGAGCCTTACGGAAAGCCTGTANACGTCTGGGGGTGCGGTGTGATCCTTTNTATCC
  TGCTCAGTGGGNTGTTGCCTTTNTACNGNNACCAAGNAAGATTTGTTGAAGGCTTATTAA
  AGGGAAATATTAGATGAATCCCAGGCAGTGGAGCCATATCTCTGAAAGTGCCAAGACCTA
  NTACGTCGCATGCTGATGCTGGATCCANCTGAAGGATCACTGTNTATGAAGCCTGAATCA
  CCATGGCTTAGGAGCGGNNATCGTACGCTACAGATCATCTTCAGAACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003688 unedited
  CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTCAAGGAAACAGAATCA
  TTGTTTATTATTATTAAAATAGATTATAGAAAGCAGCATCTATTTTGTGCAGTTTTTAGG
  GGCCTTGGAAATAAAAAGCTATGATTTCCAAAAATATAACATTCCAAAGGATTGAATAAT
  ACATACATTTAAAGATACATTTTATTAAAGTTTAGGAGAACTGATTAAAAAAGATACTGT
  CTCTTTAAATTAGGGTGTAATTGTTTTTCTCCTCCTATCTATTCGGACATGACAATAATT
  ATAAATGTAGGTCACACTACAACTAGGTAGTCTCTAGGGACCATGACCTGCTGACACAAG
  GCCGATAACAAAGAGGCTTTTCCACAAATGAGGTGCTCCCAGTTATGCTCAGATATCTGG
  GGAGAGGCCTAATAGACCCAGGAGACAGGGACCCACTGTGGGGCTGTGCACACGAGCTCA
  ACAGCTTCCTCCAGATGTCTGATTGTCTCATCAATTTCATTGTTGATAATTGGGAGATCG
  AAGTAGTGTGCATATGTTCTCTGTAAGATGTCAGACTCCTTCTGCAGACGCTGAAGAGAT
  TCATCCTCATTTAAACCTGNAGTAATAGTTGGTGCCACAATGAAAACAACAAAAGGACCA
  AACTCTGCAATTCTCAAGACCTTTAGTGCCTGAAGCTCCACGTCCAGTATTGCAATCAGC
  CCCTGCTCGTGGATCTTCCGGATGGTCTCCAGTTTTGTCCCATACATGCATCCCTGTGGT
  TGCCGTACTCCAAGACTTCGTATAAGAAATGCCTTGCATCATTTGGCCATGAGAAACAAA
  GAATAATTCCTCTCCTTTTCTTCCCTTTTTTCTGAGTCCGGGTTGATGGGAAAAAGGCAC
  CCAAACCGGTCGGGTGCCTTGGGAAAAAAAGGTTTTAATTGGCTTTTCCAAACCCTGGGC
  N
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003688
• Insert Size: 3490 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003688.1, NP_003679.1
• RefSeq Size: 3122 bp
• RefSeq ORF: 2766 bp
• Locus ID: 8573
• UniProt ID: O14936
• Cytogenetics: Xp11.4
• Domains: pkinase, TyrKc, SH3, PDZ, S_TKc, L27, Guanylate_kin, GuKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Tight junction
• Gene Summary: This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, intellectual disability and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked intellectual disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.