S1P (MBTPS1) (NM_003791) Human Untagged Clone

CAT#: SC117763

MBTPS1 (untagged)-Human membrane-bound transcription factor peptidase, site 1 (MBTPS1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: S1P
• Synonyms: PCSK8; S1P; SEDKF; SKI-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003791, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGCTTGTCAACATCTGGCTGCTTCTGCTCGTGGTTTTGCTCTGTGGGAAGAAACATCTGGGCGACA
  GACTGGAAAAGAAATCTTTTGAAAAGGCCCCATGCCCTGGCTGTTCCCACCTGACTTTGAAGGTGGAATT
  CTCATCAACAGTTGTGGAATATGAATATATTGTGGCTTTCAATGGATACTTTACAGCCAAAGCTAGAAAT
  TCATTTATTTCAAGTGCCCTGAAGAGCAGTGAAGTAGACAATTGGAGAATTATACCTCGAAACAATCCAT
  CCAGTGACTACCCTAGTGATTTTGAGGTGATTCAGATAAAAGAAAAACAGAAAGCGGGGCTGCTAACACT
  TGAAGATCATCCAAACATCAAACGGGTCACGCCCCAACGAAAAGTCTTTCGTTCCCTCAAGTATGCTGAA
  TCTGACCCCACAGTACCCTGCAATGAAACCCGGTGGAGCCAGAAGTGGCAATCATCACGTCCCCTGCGAA
  GAGCCAGCCTCTCCCTGGGCTCTGGCTTCTGGCATGCTACGGGAAGGCATTCGAGCAGACGGCTGCTGAG
  AGCCATCCCGCGCCAGGTTGCCCAGACACTGCAGGCAGATGTGCTCTGGCAGATGGGATATACAGGTGCT
  AATGTAAGAGTTGCTGTTTTTGACACTGGGCTGAGCGAGAAGCATCCCCACTTCAAAAATGTGAAGGAGA
  GAACCAACTGGACCAACGAGCGAACGCTGGACGATGGGTTGGGCCATGGCACATTCGTGGCAGGTGTGAT
  AGCCAGCATGAGGGAGTGCCAAGGATTTGCTCCAGATGCAGAACTTCACATTTTCAGGGTCTTTACCAAT
  AATCAGGTATCTTACACATCTTGGTTTTTGGACGCCTTCAACTATGCCATTTTAAAGAAGATCGACGTGT
  TAAACCTCAGCATCGGCGGCCCGGACTTCATGGATCATCCGTTTGTTGACAAGGTGTGGGAATTAACAGC
  TAACAATGTAATCATGGTTTCTGCTATTGGCAATGACGGACCTCTTTATGGCACTCTGAATAACCCTGCT
  GATCAAATGGATGTGATTGGAGTAGGCGGCATTGACTTTGAAGATAACATCGCCCGCTTTTCTTCAAGGG
  GAATGACTACCTGGGAGCTACCAGGAGGCTACGGTCGCATGAAACCTGACATTGTCACCTATGGTGCTGG
  CGTGCGGGGTTCTGGCGTGAAAGGGGGGTGCCGGGCCCTCTCAGGGACCAGTGTTGCTTCTCCAGTGGTT
  GCAGGTGCTGTCACCTTGTTAGTGAGCACAGTCCAGAAGCGTGAGCTGGTGAATCCCGCCAGTATGAAGC
  AGGCCCTGATCGCGTCAGCCCGGAGGCTCCCCGGGGTCAACATGTTTGAGCAAGGCCACGGCAAGCTCGA
  TCTGCTCAGAGCCTATCAGATCCTCAACAGCTACAAGCCACAGGCAAGTTTGAGCCCCAGCTACATAGAT
  CTGACTGAGTGTCCCTACATGTGGCCCTACTGCTCCCAGCCCATCTACTATGGAGGAATGCCGACAGTTG
  TTAATGTCACCATCCTCAACGGCATGGGAGTCACAGGAAGAATTGTAGATAAGCCTGACTGGCAGCCCTA
  TTTGCCACAGAACGGAGACAACATTGAAGTTGCCTTCTCCTACTCCTCGGTCTTATGGCCTTGGTCGGGC
  TACCTGGCCATCTCCATTTCTGTGACCAAGAAAGCGGCTTCCTGGGAAGGCATTGCTCAGGGCCATGTCA
  TGATCACTGTGGCTTCCCCAGCAGAGACAGAGTCAAAAAATGGTGCAGAACAGACTTCAACAGTAAAGCT
  CCCCATTAAGGTGAAGATAATTCCTACTCCCCCGCGAAGCAAGAGAGTTCTCTGGGATCAGTACCACAAC
  CTCCGCTATCCACCTGGCTATTTCCCCAGGGATAATTTAAGGATGAAGAATGACCCTTTAGACTGGAATG
  GTGATCACATCCACACCAATTTCAGGGATATGTACCAGCATCTGAGAAGCATGGGCTACTTTGTAGAGGT
  CCTCGGGGCCCCCTTCACGTGTTTTGATGCCAGTCAGTATGGCACTTTGCTGATGGTGGACAGTGAGGAG
  GAGTACTTCCCTGAAGAGATCGCCAAGCTCCGGAGGGACGTGGACAACGGCCTCTCGCTCGTCATCTTCA
  GTGACTGGTACAACACTTCTGTTATGAGAAAAGTGAAGTTTTATGATGAAAACACAAGGCAGTGGTGGAT
  GCCGGATACCGGAGGAGCTAACATCCCAGCTCTGAATGAGCTGCTGTCTGTGTGGAACATGGGGTTCAGC
  GATGGCCTGTATGAAGGGGAGTTCACCCTGGCCAACCATGACATGTATTATGCGTCAGGGTGCAGCATCG
  CGAAGTTTCCAGAAGATGGCGTCGTGATAACACAGACTTTCAAGGACCAAGGATTGGAGGTTTTAAAGCA
  GGAAACAGCAGTTGTTGAAAACGTCCCCATTTTGGGACTTTATCAGATTCCAGCTGAGGGTGGAGGCCGG
  ATTGTACTGTATGGGGACTCCAATTGCTTGGATGACAGTCACCGACAGAAGGACTGCTTTTGGCTTCTGG
  ATGCCCTCCTCCAGTACACATCGTATGGGGTGACACCGCCTAGCCTCAGTCACTCTGGGAACCGCCAGCG
  CCCTCCCAGTGGAGCAGGCTCAGTCACTCCAGAGAGGATGGAAGGAAACCATCTTCATCGGTACTCCAAG
  GTTCTGGAGGCCCATTTGGGAGACCCAAAACCTCGGCCTCTACCAGCCTGTCCACGCTTGTCTTGGGCCA
  AGCCACAGCCTTTAAACGAGACGGCGCCCAGTAACCTTTGGAAACATCAGAAGCTACTCTCCATTGACCT
  GGACAAGGTGGTGTTACCCAACTTTCGATCGAATCGCCCTCAAGTGAGGCCCTTGTCCCCTGGAGAGAGC
  GGCGCCTGGGACATTCCTGGAGGGATCATGCCTGGCCGCTACAACCAGGAGGTGGGCCAGACCATTCCTG
  TCTTTGCCTTCCTGGGAGCCATGGTGGTCCTGGCCTTCTTTGTGGTACAAATCAACAAGGCCAAGAGCAG
  GCCGAAGCGGAGGAAGCCCAGGGTGAAGCGCCCGCAGCTCATGCAGCAGGTTCACCCGCCAAAGACCCCT
  TCGGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003791 unedited
  GGGCACATTTGTATACGACTCCTATAGGCGGCNCGCGATTCGGCACGAGGCGGCGGAGCT
  GAGGCTCCCAGCTGTGGGCCTCGCTGGCCCGGTCGCCCAGTCTCGCGAGAGTTGGGAGTA
  AACAGCCCCGAATGGAGTGCCCAGGCGTGTTCGCCGCGGAGGCGCCGTTATCCCGGGCCC
  GCCGGCCCTGAGCTCCCGGCGGCGCAGATTGGCTCACAGTGGTTGATTGATCAACCCCAT
  TGGACGTTGGTTCTGTGGTACAAATGGAGTACAGGACTCAGTCGTCACGGCCTGAGTGAG
  AGAAGCCTTATTTCCAAGATGGAGAAGAAGCGGAGAAAGAAATGAAAGCCTCTCTTCAGG
  CTGAACCACAAAAGGCCATGGGATTTAACTTTTATTTATGTTGGGCAAGACTGTAAGATG
  GCTGATTAGTAATGTTGCAGCTTTTAGCTGAAACAAAAATTCACTTTTAATCAAGAAGAA
  AAAAGTGTGATTTGAATATATGCAATTTTATGATCATATTCGCTTGTGACCATGAAGCTT
  GTCAACATCTGGCTGCTTCTGCTCGTGGTTTTGCTCTGTGGGAAGAAACATCTGGGCGAC
  AGACTGGAAAAGAAATCTTTTGAAAAGGCCCCATGCCCTGNCTGTTCCCACCTGACTNTG
  AAGGTGGAATTCTCATCAACAGTTGTGGAATATGAATATATTGTGGCTTTCAATGGATAC
  TNTACAGCCAAAGCTAGAAATTCATTTATTTCAAGTGCCCCTGAGAGCAGTGAAGTAGAC
  AATTGGAGAAATATACCTCGAANCAATNCATCCAGTGACTACCCTAGTGATTTTGAGGGT
  GATCAGATAAAGAAAAACAGAAAGCGGGCTGCTAACACTTGAAGACATNCAANCATCAAC
  CGGTCACGCCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003791 unedited
  TGCACCGCGGCACGCAATCTAGCATCGAGTTTTTTTTTTTTTTTTTTAAGCTTCAAACGG
  GTAATACATATTTATTGAAAATTTTCTTCACCGACAATGGTGAAATCAAGACCTCAAATT
  ACAAAACATGGCGGCAGGTGATACTTACAAAAATAAAGCGAAGGTCTATGTTTTACAGAT
  TAGTGCATGTTACCTTTAAATCTCAGCCTGTCCTGCCATTAAAAAGCATCATGGTATCTA
  TGTTGTCTCTTATGATGGATTACCCAAAAAACTGCATTTCACTGACAAAAAAAATATCTT
  TGCTTGTAAATAGCACAATTTTTTAAAGCGACTTTTTTCATCAATAAATATAGAAAATAT
  CCTTCACCACTCGCCCTTAACCTTGCTCACGTTTGTCTTATTTGTCCGCAAAGCCATCCC
  TTTCCCTGTTGTATCACACTGCTCACACTCTCTTTTCTACTTCCACCTATTCTTTGGTGT
  CATCACTCTTTCGACCGACAATTCTTACCGGTGGCTCAATCACTAAATTCCTTCTGTCGG
  TATCTTTAAGATTCACTCCTCATGTTCATCTACCCCTCTGTACTTTAAAGATGCTCCTTC
  CTGCTTAATATATCTTATCTATTTTTATCACATAAACTGTTTTATTTTAATACTTTCTAT
  AACCACGCTCCGTTACACCATTTTTTGTTCCTATCTTATATGCCACATCTTTGGTTCCTC
  CCCGCCCACTCCTTCTTCCCTTTTTATCTCTCTAGGGTATTATTTTCAGTCGTACCTCAT
  TCATTTGCACCCTCCCCTCCTCCTCCCATTGATTTTCCTCTCTTATCCCCTTCCCCCGAC
  TTCTCAATATTTTTATTCCCGCATTAGCCCTTATTTCTTTCGTTTTTTCTTCACTCTCTC
  ACCGTATATAATCGACTTCCCTTCTCTTTTTTTATTTATTCTCAACTCCCCACACGTGAA
  TACCCTGTCCCTACCACTGGTCATAGTTTATTCCCTTGTTTATTATCTTTCTCTCCCCAA
  TTCACATATTCATTCTACTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003791
• Insert Size: 4650 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003791.2, NP_003782.1
• RefSeq Size: 4354 bp
• RefSeq ORF: 3159 bp
• Locus ID: 8720
• UniProt ID: Q14703
• Cytogenetics: 16q23.3-q24.1
• Domains: Peptidase_S8
• Protein Families: Druggable Genome, Protease, Transcription Factors, Transmembrane
• Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an initial autocatalytic processing event in the ER to generate a heterodimer which exits the ER and sorts to the cis/medial-Golgi where a second autocatalytic event takes place and the catalytic activity is acquired. It encodes a type 1 membrane bound protease which is ubiquitously expressed and regulates cholesterol or lipid homeostasis via cleavage of substrates at non-basic residues. Mutations in this gene may be associated with lysosomal dysfunction. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).