SOCS3 (NM_003955) Human Untagged Clone

CAT#: SC117660

SOCS3 (untagged)-Human suppressor of cytokine signaling 3 (SOCS3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SOCS3
• Synonyms: ATOD4; CIS3; Cish3; SOCS-3; SSI-3; SSI3
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003955 edited
  GGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTATA
  GAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGGCTGCCGCTT
  CGGCCCCGCACGCAGCCAGCCGCCAGCCGCCCGCCCGGCCCAGCTCCCGCCGCGGCCCCT
  TGCCGCGGTCCCTCTCCTGGTCCCCTCCCGGTTGGTCCGGGGGTGCGCAGGGGGCAGGGC
  GGGCGCCCAGGGGAAGCTCGAGGGACGCGCGCGCGAAGGCTCCTTTGTGGACTTCACGGC
  CGCCAACATCTGGGCGCAGCGCGGGCCACCGCTGGCCGTCTCGCCGCCGCGTCGCCTTGG
  GGACCCGAGGGGGCTCAGCCCCAAGGACGGAGACTTCGATTCGGGACCAGCCCCCCGGGA
  TGCGGTAGCGGCCGCTGTGCGGAGGCCGCGAAGCAGCTGCAGCCGCCGCCGCGCAGATCC
  ACGCTGGCTCCGTGCGCCATGGTCACCCACAGCAAGTTTCCCGCCGCCGGGATGAGCCGC
  CCCCTGGACACCAGCCTGCGCCTCAAGACCTTCAGCTCCAAGAGCGAGTACCAGCTGGTG
  GTGAACGCAGTGCGCAAGCTGCAGGAGAGCGGCTTCTACTGGAGCGCAGTGACCGGCGGC
  GAGGCGAACCTGCTGCTCAGTGCCGAGCCCGCCGGCACCTTTCTGATCCGCGACAGCTCG
  GACCAGCGCCACTTCTTCACGCTCAGCGTCAAGACCCAGTCTGGGACCAAGAACCTGCGC
  ATCCAGTGTGAGGGGGGCAGCTTCTCTCTGCAGAGCGATCCCCGGAGCACGCAGCCCGTG
  CCCCGCTTCGACTGCGTGCTCAAGCTGGTGCACCACTACATGCCGCCCCCTGGAGCCCCC
  TCCTTCCCCTCGCCACCTACTGAACCCTCCTCCGAGGTGCCCGAGCAGCCGTCTGCCCAG
  CCACTCCCTGGGAGTCCCCCCAGAAGAGCCTATTACATCTACTCCGGGGGCGAGAAGATC
  CCCCTGGTGTTGAGCCGGCCCCTCTCCTCCAACGTGGCCACTCTTCAGCATCTCTGTCGG
  AAGACCGTCAACGGCCACCTGGACTCCTATGAGAAAGTCACCCAGCTGCCGGGGCCCATT
  CGGGAGTTCCTGGACCAGTACGATGCCCCGCTTTAAGGGGTAAAGGGCGCAAAGGGCATG
  GGTCGGGAGAGGGGACGCAGGCCCCTCTCCTCCGTGGCACATGGCACAAGCACAAGAAGC
  CAACCAGGAGAGAGTCCTGTAGCTCTGGGGGGAAAGAGGGCGGACAGGCCCCTCCCTCTG
  CCCTCTCCCTGCAGAATGTGGCAAGCGGACCTGGAATGTGTTGGAGGGAAGGGGGAGTAC
  CACCTGAGTCTCCAGCTTCTCCGGAAGAGCCAGCTGTCCTGGTGGGACGATAGCAACCAC
  AAGTGGATTCTCCTTCAATTCCTCAGCTTCCCCTCTG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003955 unedited
  NNTTTCTTTCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTAT
  ATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCG
  GCCGCGAATTCGGCACGAGGGCTGCCGCTTCGGCCCCGCACGCAGCCAGCCGCCAGCCGC
  CCGCCCGGCCCAGCTCCCGCCGCGGCCCCTTGCCGCGGTCCCTCTCCTGGTCCCCTCCCG
  GTTGGTCCGGGGGTGCGCAGGGGGCAGGGCGGGCGCCCAGGGGAAGCTCGAGGGACGCGC
  GCGCGAAGGCTCCTTTGTGGACTTCACGGCCGCCAACATCTGGGCGCAGCGCGGGCCACC
  GCTGGCCGTCTCGCCGCCGCGTCGCCTTGGGGACCCGAGGGGGCTCAGCCCCAAGGACGG
  AGACTTCGATTCGGGACCAGCCCCCCGGGATGCGGTAGCGGCCGCTGTGCGGAGGCCGCG
  AAGCAGCTGCAGCCGCCGCCGCGCAGATCCACGCTGGCTCCGTGCGCCATGGTCACCCAC
  AGCAAGTTTCCCGCCGCCGGGATGAGCCGCCCCCTGGACACCAGCCTGCGCCTCAAGACC
  TTCAGCTCCAAGAGCGAGTACCAGCTGGTGGTGAACGCAGTGCGCAAGCTGCAGGAGAGC
  GGCTTCTACTGGAGCGCAGTGACCGGCGGCGAGGCGAACCTGCTGCTCAGTGCCGAGCCC
  GCCGGCACCTTTCTGATCCGCGACAGCTCGGACCAGCGCCACTTCTTCACGCTCAGCGTC
  AAGACCCAGTCTGGGACCAAGAACCTGCGCATCCAGTGTGGAGGGGGGCAGCTTCTCTCT
  GCAGAGCGATCCCCGGAGCACGCAGCCCGTGCCCCGCTTCGACTGCGTGCTCAAGCTGGT
  GCACCAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003955
• Insert Size: 2900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003955.3, NP_003946.3
• RefSeq Size: 2746 bp
• RefSeq ORF: 678 bp
• Locus ID: 9021
• UniProt ID: O14543
• Cytogenetics: 17q25.3
• Domains: SH2, SOCS
• Protein Families: Druggable Genome
• Protein Pathways: Adipocytokine signaling pathway, Insulin signaling pathway, Jak-STAT signaling pathway, Type II diabetes mellitus, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon (IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development. [provided by RefSeq, Jul 2008]