DDX3Y (NM_004660) Human Untagged Clone

CAT#: SC117258

DDX3Y (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, Y-linked (DDX3Y), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX3Y
• Synonyms: DBY
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117258 sequence for NM_004660 edited (data generated by NextGen Sequencing)
  ATGAGTCATGTGGTGGTGAAAAATGACCCTGAACTGGACCAGCAGCTTGCTAATCTGGAC
  CTGAACTCTGAAAAACAGAGTGGAGGAGCAAGTACAGCGAGCAAAGGGCGCTATATACCT
  CCTCACTTAAGGAACAGAGAAGCATCTAAAGGATTCCATGATAAAGACAGTTCAGGTTGG
  AGTTGCAGCAAAGATAAGGATGCATATAGCAGTTTTGGGTCTCGAGATTCTAGAGGAAAG
  CCTGGTTATTTCAGTGAACGTGGAAGTGGATCAAGGGGAAGATTTGATGATCGTGGACGG
  AGTGACTATGATGGTATTGGCAATCGTGAAAGACCTGGCTTTGGCAGATTTGAACGGAGT
  GGACATAGTCGTTGGTGTGACAAGTCAGTTGAAGATGATTGGTCAAAACCACTTCCACCA
  AGTGAACGCTTGGAGCAAGAACTGTTTTCTGGAGGAAACACGGGGATTAACTTTGAGAAA
  TATGATGATATACCAGTAGAGGCAACCGGCAGTAACTGTCCTCCACATATTGAGAATTTT
  AGCGATATTGACATGGGAGAAATTATCATGGGGAACATTGAACTTACTCGCTATACTCGT
  CCTACTCCAGTGCAAAAACATGCCATTCCTATTATTAAGGGAAAAAGAGACTTAATGGCT
  TGTGCCCAAACAGGATCTGGGAAAACTGCAGCATTTCTTTTACCCATACTGAGTCAGATA
  TATACAGATGGTCCAGGAGAAGCTTTGAAGGCTGTGAAGGAAAATGGAAGGTATGGGCGC
  CGCAAACAATATCCAATATCCTTGGTTTTAGCCCCAACAAGAGAATTGGCTGTACAGATC
  TATGAGGAAGCCAGAAAATTTTCCTACCGATCTAGAGTTCGTCCTTGTGTAGTTTATGGT
  GGTGCTGATATTGGTCAGCAGATTCGGGACTTAGAACGTGGATGCCACTTGTTAGTAGCC
  ACTCCAGGACGTCTAGTGGATATGATGGAAAGAGGAAAGATTGGATTAGACTTCTGCAAG
  TACTTAGTGTTGGATGAAGCTGATAGGATGCTGGATATGGGATTTGAACCTCAGATACGT
  CGTATAGTTGAACAAGATACTATGCCACCAAAGGGCGTTCGTCACACCATGATGTTTAGT
  GCTACTTTTCCTAAGGAAATACAGATGCTTGCTCGTGACTTTTTGGATGAATATATCTTT
  TTGGCTGTAGGCAGAGTAGGCTCTACCTCTGAGAACATCACACAGAAAGTAGTTTGGGTG
  GAAGACTTAGATAAACGGTCATTTCTACTGGACATTTTAGGTGCAACAGGGAGTGATTCA
  CTTACTTTAGTGTTTGTGGAGACCAAAAAGGGAGCAGATTCCCTGGAGGATTTCTTATAC
  CATGAAGGATATGCTTGTACTAGTATTCATGGAGACCGGTCACAGAGAGATCGAGAGGAG
  GCCCTTCACCAGTTTCGCTCAGGAAAAAGCCCAATTCTAGTGGCTACAGCTGTGGCAGCA
  CGAGGACTAGACATTTCAAATGTGAGACATGTTATCAATTTTGATTTGCCAAGTGATATT
  GAAGAATATGTGCATCGTATTGGCCGTACAGGACGTGTAGGAAACCTGGGCCTTGCCACC
  TCATTCTTTAATGAAAAAAATATGAATATTACAAAGGATTTGTTGGATCTTCTTGTAGAA
  GCTAAACAAGAAGTGCCTTCTTGGTTGGAAAATATGGCTTATGAACACCACTACAAGGGT
  GGCAGTCGTGGACGATCTAAAAGTAATAGATTCAGTGGAGGATTTGGTGCCAGAGACTAT
  CGACAAAGTAGTGGTTCCAGCAGTTCTGGCTTTGGTGCTAGTCGCGGAAGCAGCAGCCGC
  AGTGGTGGAGGTGGTTACGGCAACAGCAGAGGATTTGGTGGAGGTGGCTATGGAGGCTTC
  TACAATAGTGATGGATATGGAGGAAATTATAACTCCCAGGGGGTTGACTGGTGGGGCAAC
  TGA
  
  Clone variation with respect to NM_004660.3
  
• Restriction Sites: Please inquire
• ACCN: NM_004660
• Insert Size: 4110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004660.2, NP_004651.2
• RefSeq Size: 4416 bp
• RefSeq ORF: 1983 bp
• Locus ID: 8653
• UniProt ID: O15523
• Cytogenetics: Yq11.221
• Domains: DEAD, helicase_C
• Protein Pathways: RIG-I-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (2) differs in the 5' and 3' UTRs compared to variant 1. Both variants 1 and 2 encode the same isoform (1).