DDX3Y Rabbit Polyclonal Antibody

CAT#: TA375393

DDX3Y Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of various cell lines, using DDX3Y antibody (TA375393) at 1:3000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 1s.

• 

  Immunofluorescence analysis of C6 cells using DDX3Y Polyclonal Antibody (TA375393) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of L929 cells using DDX3Y Polyclonal Antibody (TA375393) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

• 

  Immunofluorescence analysis of U-2 OS cells using DDX3Y Polyclonal Antibody (TA375393) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, IHC, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IHC,1:50 - 1:100
  IF,1:50 - 1:100
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human DDX3Y (NP_001116137.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 32kDa/72kDa/73kDa
• Gene Name: DEAD-box helicase 3, Y-linked
• Database Link:
  Entrez Gene 8653 Human
  UniProt ID O15523
• Background: The protein encoded by this gene is a member of the DEAD-box RNA helicase family, characterized by nine conserved motifs, included the conserved Asp-Glu-Ala-Asp (DEAD) motif. These motifs are thought to be involved in ATP binding, hydrolysis, RNA binding, and in the formation of intramolecular interactions. This protein shares high similarity to DDX3X, on the X chromosome, but a deletion of this gene is not complemented by DDX3X. Mutations in this gene result in male infertility, a reduction in germ cell numbers, and can result in Sertoli-cell only sydrome. Pseudogenes sharing similarity to both this gene and the DDX3X paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Synonyms: DBY