SLC33A1 (NM_004733) Human Untagged Clone

CAT#: SC117182

SLC33A1 (untagged)-Human solute carrier family 33 (acetyl-CoA transporter), member 1 (SLC33A1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC33A1
• Synonyms: ACATN; AT-1; AT1; CCHLND; SPG42
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117182 sequence for NM_004733 edited (data generated by NextGen Sequencing)
  ATGTCACCCACCATCTCCCACAAGGACAGCAGCCGGCAACGGCGGCCAGGGAATTTCAGT
  CACTCTCTGGATATGAAGAGCGGTCCCCTGCCGCCAGGCGGTTGGGATGACAGTCATTTG
  GACTCAGCGGGCCGGGAAGGGGACAGAGAAGCTCTTCTGGGGGATACCGGCACTGGCGAC
  TTCTTAAAAGCCCCACAGAGCTTCCGGGCCGAACTAAGCAGCATTTTGCTACTACTCTTT
  CTTTACGTGCTTCAGGGTATTCCCCTGGGCTTGGCGGGAAGCATCCCACTCATTTTGCAA
  AGCAAAAATGTTAGCTATACAGACCAAGCTTTCTTCAGTTTTGTCTTTTGGCCCTTCAGT
  CTCAAATTACTCTGGGCCCCGTTGGTTGATGCGGTCTACGTTAAGAACTTCGGTCGTCGC
  AAATCTTGGCTTGTCCCGACACAGTATATACTAGGACTCTTCATGATCTATTTATCCACT
  CAGGTGGACCGTTTGCTTGGGAATACCGATGACAGAACACCCGACGTGATTGCTCTCACT
  GTGGCGTTCTTTTTGTTTGAATTCTTGGCCGCCACTCAGGACATTGCCGTCGATGGTTGG
  GCGTTAACTATGTTATCCAGGGAAAATGTGGGTTATGCTTCTACTTGCAATTCGGTGGGC
  CAAACAGCGGGTTACTTTTTGGGCAATGTTTTGTTTTTGGCCCTTGAATCTGCCGACTTT
  TGTAACAAATATTTGCGGTTTCAGCCTCAACCCAGAGGAATCGTTACTCTTTCAGATTTC
  CTTTTTTTCTGGGGAACTGTATTTTTAATAACAACAACATTGGTTGCCCTTCTGAAAAAA
  GAAAACGAAGTATCAGTAGTAAAAGAAGAAACACAAGGGATCACAGATACTTACAAGCTG
  CTTTTTGCAATTATAAAAATGCCAGCAGTTCTGACATTTTGCCTTCTGATTCTAACTGCA
  AAGATTGGTTTTTCAGCAGCAGATGCTGTAACAGGACTGAAATTGGTAGAAGAGGGAGTA
  CCCAAAGAACATTTAGCCTTATTGGCAGTTCCAATGGTTCCTTTGCAGATAATACTGCCT
  CTGATTATCAGCAAATACACTGCAGGTCCCCAGCCATTAAACACATTTTACAAAGCCATG
  CCCTACAGATTATTGCTTGGGTTAGAATATGCCCTACTGGTTTGGTGGACTCCTAAAGTA
  GAACATCAAGGGGGATTCCCTATATATTACTATATCGTAGTCCTGCTGAGTTATGCTTTA
  CATCAGGTTACAGTGTACAGCATGTATGTTTCTATAATGGCTTTCAATGCAAAGGTTAGT
  GATCCACTTATTGGAGGAACATACATGACCCTTTTAAATACCGTGTCCAATCTGGGAGGA
  AACTGGCCTTCTACAGTAGCTCTTTGGCTTGTAGATCCCCTCACAGTAAAAGAGTGTGTA
  GGAGCATCAAACCAGAATTGTCGAACACCTGATGCTGTTGAGCTTTGCAAAAAACTGGGT
  GGCTCATGTGTTACAGCCCTGGATGGTTATTATGTGGAGTCCATTATTTGTGTTTTCATT
  GGATTTGGTTGGTGGTTCTTTCTTGGTCCAAAATTTAAAAAGTTACAGGATGAAGGATCA
  TCTTCGTGGAAATGCAAAAGGAACAATTAA
  
  Clone variation with respect to NM_004733.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004733 unedited
  NGTTCACATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCAGC
  CGTTCGATCCCGCCGCAGGTAGGAGCTGGTTTCCATCCTGGCACCACGGCACACACCTCC
  AGCCTCGAGCCCGGCGCTGCTGCCCGGGGGTCTCCTTCAGGCTCTTTGACGCCGTTCCAG
  GGGGCACCTATCCAGGCATCCTCTGGGCCTCTAGCCAGAGGACTGGCTCCCGGCTTCAGC
  ACTCCGGGCTGCAGTAAGAAGTGCCCTTATCGCTCTGAGCCCTGCCACCATCCCGTGAAC
  CACCGAAACCCTGGTCCAGCGCGACAGCCTTGGACCTGGGACTGGACGGATCCAAAACGC
  TCAGCCTCGGCCCCCCACAGACGGGGCTCTGCATCGTCTCTGATATGTCACCCACCATCT
  CCCACAAGGACAGCAGCCGGCAACGGCGGCCAGGGAATTTCAGTCACTCTCTGGATATGA
  AGAGCGGTCCCCTGCCGCCAGGCGGTTGGGATGACAGTCATTTGGACTCAGCGGGCCGGG
  AAGGGGACAGAGAAGCTCTTCTGGGGGATACCGGCACTGGCGACTTCTTAAAAGCCCCAC
  AGAGCTTCCGGGCCGAACTAAGCAGCATTTTGCTACTACTCTTTCTTTACGTGCTTCAGG
  GTATTCCCCTGGGCTTGGCGGGAAGCATCCCACTCATTTTGCANAGCANAAATGTTAGCT
  ATACAGACCAAGCTTTCTTCAGTTTTGTCTTTTGGCCCTTCAGTCTCAAATTACTCTGGG
  CCCCGTTGGTTGATGNCGTCTACGTTAAGAACTTCGGTCGTCGCAATCTTGGCTTGTCCC
  GCACAGTATATACTANGACTCTTCATGATCTATTTATCCACTCAGGTGGACCGTTTGCTT
  GNGAATACCGATGACAGACACCCGACGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004733
• Insert Size: 2640 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004733.2, NP_004724.1
• RefSeq Size: 2659 bp
• RefSeq ORF: 1650 bp
• Locus ID: 9197
• UniProt ID: O00400
• Cytogenetics: 3q25.31
• Protein Families: Transmembrane
• Protein Pathways: Glycosphingolipid biosynthesis - ganglio series, Metabolic pathways
• Gene Summary: The protein encoded by this gene is required for the formation of O-acetylated (Ac) gangliosides. The encoded protein is predicted to contain 6 to 10 transmembrane domains, and a leucine zipper motif in transmembrane domain III. Defects in this gene have been reported to cause spastic paraplegia autosomal dominant type 42 (SPG42) in one Chinese family, but not in similar patients of European descent. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.