RAC3 (NM_005052) Human Untagged Clone

CAT#: SC116963

RAC3 (untagged)-Human ras-related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) (RAC3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAC3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116963 sequence for NM_005052 edited (data generated by NextGen Sequencing)
  ATGCAGGCCATCAAGTGCGTGGTGGTCGGCGACGGCGCCGTGGGGAAGACATGCTTGCTG
  ATCAGCTACACGACCAACGCCTTCCCCGGAGAGTACATCCCCACCGTTTTTGACAACTAC
  TCTGCCAACGTGATGGTGGACGGGAAACCAGTCAACTTGGGGCTGTGGGACACAGCGGGT
  CAGGAGGACTACGATCGGCTGCGGCCACTCTCCTACCCCCAAACTGACGTCTTTCTGATC
  TGCTTCTCTCTGGTGAGCCCGGCCTCCTTCGAGAATGTTCGTGCCAAGTGGTACCCGGAG
  GTGCGGCACCACTGCCCCCACACGCCCATCCTCCTGGTGGGCACCAAGCTGGACCTCCGC
  GACGACAAGGACACCATTGAGCGGCTGCGGGACAAGAAGCTGGCACCCATCACCTACCCA
  CAGGGCCTGGCCATGGCCCGGGAGATTGGCTCTGTGAAATACCTGGAGTGCTCAGCCCTG
  ACCCAGCGGGGCCTGAAGACAGTGTTTGACGAGGCGATCCGCGCGGTGCTCTGCCCGCCC
  CCAGTGAAGAAGCCGGGGAAGAAGTGCACCGTCTTCTAG
  
  Clone variation with respect to NM_005052.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005052 unedited
  NGGTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCCGCTGCG
  GCGCCGGGCATTTCTCCGCAGCTCGGCTCGCGGCCGCGCCCGCCGCCGCCCGGCCCGCGC
  CCATGCAGGCCATCAAGTGCGTGGTGGTCGGCGACGGCGCCGTGGGGAAGACATGCTTGC
  TGATCAGCTACACGACCAACGCCTTCCCCGGAGAGTACATCCCCACCGTTTTTGACAACT
  ACTCTGCCAACGTGATGGTGGACGGGAAACCAGTCAACTTGGGGCTGTGGGACACAGCGG
  GTCAGGAGGACTACGATCGGCTGCGGCCACTCTCCTACCCCCAAACTGACGTCTTTCTGA
  TCTGCTTCTCTCTGGTGAGCCCGGCCTCCTTCGAGAATGTTCGTGCCAAGTGGTACCCGG
  AGGTGCGGCACCACTGCCCCCACACGCCCATCCTCCTGGTGGGCACCAAGCTGGACCTCC
  GCGACGACAAGGACACCATTGAGCGGCTGCGGGACAAGAAGCTGGCACCCATCACCTACC
  CACAGGGCCTGGCCATGGCCCGGGAGATTGGCTCTGTGAAATACCTGGAGTGCTCAGCCC
  TGACCCAGCGGGGCCTGAAGACAGTGTTTGACGAGGCGATCCGCGCGGTGCTCTGCCCGC
  CCCCAGTGAAGAAGCCGGNGAAGAAGTGCACCGTCTTCTAGAGCCCTGGCCCCACCCGAG
  CCTGAGGCTGGCGGNGAGCAGCCCTGGACGTGTCCGCTGTTGTGTTGAGACGTGTGGTGT
  CCCTGNAGTCGCTGTGGNGAACGTGGGGGGTTGGCCCGGNGGGAANCATGGGGATGAAGC
  TGGNTTGGCAGATCCTGTCCTCTCTGCCGCCTCATCTTGGGGTGTGGCTCCAGCCTTCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005052 unedited
  TATGTTAAAAGAGAGGGGCTGCCCCACCTCGGGGCACAAGGAACAGAACTCCCCCCCACC
  CTCCCCCTCCAGGGAAGCCCATCCTGGGGCTTCCCTGCACCTGCCCCTGCAGCCCTGAGG
  GAGACCCTGCTCCCTCCCGGCCTCCGGCGGGGGCCAGGGAAGGCTGGAGCCACACCCCAG
  AATGAGGCGGCAGAGAGGACAGGATCCTCCCACCCAGCCTCATCCCCATGCTTCCCCCCG
  GCCCACCCCCACCGCTCCCCACAGCCGACTCAGGGACACCACACGTCTCAACACAACAGC
  GGACACGTCCAGGGCTGCTCCCCGCCAGCCCTCAGGCTCGGGTGGGCCAGGGCTCTAGAA
  GACGGTGCACTTCTTCCCCGGCTTCTTCACTGGGGGCGGGCAGAGCACCGCGCGGATCGC
  CTCGTCAAACACTGTCTTCAGGCCCCGCTGGGTCAGGGCTGAGCACTCCAGGTATTTCAC
  AGAGCCAATCTCCCGGGCCATGGCCAGGCCCTGTGGGTAGGTGATGGGTGCCAGCTTCTT
  GTCCCGCAGCCGCTCAATGGTGTCCTTGTCGTCCCGGAGTCCAGCTTGGTGCCACCAGGA
  GGATGGCGTGTGGCGCAGTGGTGCCCCACTCCGGGACCACTGGGACGAACATTCTCAAGA
  GGCCGGCTCACATAGAGACCATATCAAAAGACCTACTTGGGGGGAGAGAGGGCCCGAACC
  ATCTGATCCTCCTGCCCGCTGCGGCCACAGCCCAAGTGACTGGTTCCCGCCCCCTACGTT
  GGCAAGTCCCGCAAAACCGGGCGATTACCTCCGGGGAAGCGCGGCCCGCAGCGATCAAAC
  CAGTTCTCCCCGGGGCGTCGCACCCCCCCACTTAGGTCCCATGGCCCGGCCGGCGGTGGG
  CGCCCGCCCCACCAACTGGGAAAATCCCGCGCCAAGCGCTCCGTGAATTCCGCGCCCTTT
  TGGGCGTTTAAAATTCCCGGACCTAACAGCTCGCTATAACCTCACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005052
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005052.2, NP_005043.1
• RefSeq Size: 1077 bp
• RefSeq ORF: 579 bp
• Locus ID: 5881
• UniProt ID: P60763
• Cytogenetics: 17q25.3
• Domains: ras, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Axon guidance, B cell receptor signaling pathway, Colorectal cancer, Fc epsilon RI signaling pathway, Focal adhesion, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Pancreatic cancer, Pathways in cancer, Regulation of actin cytoskeleton, VEGF signaling pathway, Viral myocarditis, Wnt signaling pathway
• Gene Summary: The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
  Transcript Variant: This variant (1) encodes the longer isoform (1).