COX6A2 (NM_005205) Human Untagged Clone

CAT#: SC116905

COX6A2 (untagged)-Human cytochrome c oxidase subunit VIa polypeptide 2 (COX6A2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COX6A2
• Synonyms: COX6AH; COXVIAH; MC4DN18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005205 edited
  ATGGCTTTGCCTCTGAGGCCCCTGACCCGGGGCTTGGCCAGCGCTGCCAAAGGAGGCCAC
  GGAGGAGCAGGAGCTCGTACCTGGCGTCTGCTGACCTTCGTGCTGGCGCTGCCCAGCGTG
  GCCCTCTGCACCTTCAACTCCTATCTCCACTCGGGCCACCGCCCGCGCCCCGAGTTCCGT
  CCCTACCAACACCTCCGCATCCGCACCAAGCCCTACCCCTGGGGGGACGGCAACCACACT
  CTGTTCCACAATAGCCACGTGAACCCTCTGCCCACGGGCTACGAACACCCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005205 unedited
  NGTCAAAATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGATCATGGCT
  TTGCCTCTGAGGCCCCTGACCCGGGGCTTGGCCAGCGCTGCCAAAGGAGGCCACGGAGGA
  GCAGGAGCTCGTACCTGGCGTCTGCTGACCTTCGTGCTGGCGCTGCCCAGCGTGGCCCTC
  TGCACCTTCAACTCCTATCTCCACTCGGGCCACCGCCCGCGCCCCGAGTTCCGTCCCTAC
  CAACACCTCCGCATCCGCACCAAGCCCTACCCCTGGGGGGACGGCAACCACACTCTGTTC
  CACAATAGCCACGTGAACCCTCTGCCCACGGGCTACGAACACCCCTGAGGCCCCGGACGC
  CCCCGGACACAATAAAGGTGTGAAGCTTCGAAAAAAAAAAAAAAAAAACTCGACTCTAGA
  TTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCT
  CCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAA
  TAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGA
  GGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCT
  ATTGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCT
  GGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGNTGTTGGGGATTCCAGGCATGCATG
  ACCAGGCTCAGCTAATTTTTGGTTTTTTGGTAGAAACCGGGTTTCACCATATGGNCCAGG
  CTGGTCTCCAACTCCTATCTCAGGTGATCTACCCACCTTGGCCTNCCANNATGCTGGGAT
  ACAGGCGTGAACCCACTGCTCCTTNCCTGTNCCTCTGATTTTAAATACTTACCACGGGGA
  GGACGTTCAGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005205 unedited
  GAGTCGAGTTTTTTTTTTTTTTTTTTCGAAGCTTCACACCTTTATTGTGTCCGGGGGCGT
  CCGGGGCCTCAGGGGTGTTCGTAGCCCGTGGGCAGAGGGTTCACGTGGCTATTGTGGAAC
  AGAGTGTGGTTGCCGTCCCCCCAGGGGTAGGGCTTGGTGCGGATGCGGAGGTGTTGGTAG
  GGACGGAACTCGGGGCGCGGGCGGTGGCCCGAGTGGAGATAGGAGTTGAAGGTGCAAAGG
  GCCACGCTGGGCAGCGCCAGCACGAAGGTCAGCAGACGCCAGGTACGAGCTCCTGCTCCT
  CCGTGGCCTCCTTTGGCAGCGCTGGCCAAGCCCCGGGTCAGGGGCCTCAGAGGCAAAGCC
  ATGATCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAATTCTGACGG
  TTCACTAAACGAGCTCTGCTTATATAGACCTTCCACCGTACCGCCTACCGCCCATTTTGG
  TCAACGGGGCGGGGTTATTACACCTTTGGAAAGACCGTTGATTTTGTGGCAAAACAACTC
  CATTGACGCAATGGGGTGGCACTCGCAATTCTTTGAACAAACCCGTTAACCCCTTTGTGC
  CCTGGCAACCCCCTCTCTTGTGTTTACCTACTCCCCCCTATTTTTGCACTTACCAATCTC
  TCCGCCCCCTCTCGCTTATGCCCCACCCCTTTCTCCCTATTCTTTCCTCCGGTGCCCCAC
  CCCTTTTCCCCCCCCCTTTCCCCCTCCCCCCATTTTTCTCTTCCTCCCCCTCCCCTTACT
  TTATTTTTCTTTTTTCACCGTCCCCCCCCCTTTCCGCCCAACCGCCGAACGCTGGAGTAT
  TTTTTTTTTTTCCCTTCCTTAAACGCACTTTCANAAGTTCCCGCCCCGCACCTCACTCTC
  TTTCCTTCCTCCTTATTCACTCCCCTATCGCCCCCGCATCCTCAGCCCATGTTCTTCCCT
  TCACCCTCTCTCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005205
• Insert Size: 430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005205.2, NP_005196.1
• RefSeq Size: 425 bp
• RefSeq ORF: 294 bp
• Locus ID: 1339
• UniProt ID: Q02221
• Cytogenetics: 16p11.2
• Domains: COX6A
• Protein Pathways: Alzheimer's disease, Cardiac muscle contraction, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity. [provided by RefSeq, Jul 2008]