VAV1 (NM_005428) Human Untagged Clone

CAT#: SC116729

VAV1 (untagged)-Human vav 1 guanine nucleotide exchange factor (VAV1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAV1
• Synonyms: VAV
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116729 sequence for NM_005428 edited (data generated by NextGen Sequencing)
  ATGGAGCTGTGGCGCCAATGCACCCACTGGCTCATCCAGTGCCGGGTGCTGCCGCCCAGC
  CACCGCGTGACCTGGGATGGGGCTCAGGTGTGTGAACTGGCCCAGGCCCTCCGGGATGGT
  GTCCTTCTGTGTCAGCTGCTTAACAACCTGCTACCCCATGCCATCAACCTGCGTGAGGTC
  AACCTGCGCCCCCAGATGTCCCAGTTCCTGTGCCTTAAGAACATTAGAACCTTCCTGTCC
  ACCTGCTGTGAGAAGTTCGGCCTCAAGCGGAGCGAGCTCTTCGAAGCCTTTGACCTCTTC
  GATGTGCAGGATTTTGGCAAGGTCATCTACACCCTGTCTGCTCTGTCCTGGACCCCGATC
  GCCCAGAACAGGGGGATCATGCCCTTCCCCACCGAGGAGGAGAGTGTAGGTGATGAAGAC
  ATCTACAGTGGCCTGTCCGACCAGATCGACGACACGGTGGAGGAGGATGAGGACCTGTAT
  GACTGCGTGGAGAATGAGGAGGCGGAAGGCGACGAGATCTATGAGGACCTCATGCGCTCG
  GAGCCCGTGTCCATGCCGCCCAAGATGACAGAGTATGACAAGCGCTGCTGCTGCCTGCGG
  GAGATCCAGCAGACGGAGGAGAAGTACACTGACACGCTGGGCTCCATCCAGCAGCATTTC
  TTGAAGCCCCTGCAACGGTTCCTGAAACCTCAAGACATTGAGATCATCTTTATCAACATT
  GAGGACCTGCTTCGTGTTCATACTCACTTCCTAAAGGAGATGAAGGAAGCCCTGGGCACC
  CCTGGCGCAGCCAATCTCTACCAGGTCTTCATCAAATACAAGGAGAGGTTCCTCGTCTAT
  GGCCGCTACTGCAGCCAGGTGGAGTCAGCCAGCAAACACCTGGACCGTGTGGCCGCAGCC
  CGGGAGGACGTGCAGATGAAGCTGGAGGAATGTTCTCAGAGAGCCAACAACGGGAGGTTC
  ACCCTGCGGGACCTGCTGATGGTGCCTATGCAGCGAGTTCTCAAATATCACCTCCTTCTC
  CAGGAGCTGGTGAAACACACGCAGGAGGCGATGGAGAAGGAGAACCTGCGGCTGGCCCTG
  GATGCCATGAGGGACCTGGCTCAGTGCGTGAACGAGGTCAAGCGAGACAACGAGACACTG
  CGACAGATCACCAATTTCCAGCTGTCCATTGAGAACCTGGACCAGTCTCTGGCTCACTAT
  GGCCGGCCCAAGATCGACGGGGAACTCAAGATCACCTCGGTGGAACGGCGCTCCAAGATG
  GACAGGTATGCCTTCCTGCTCGACAAAGCTCTACTCATCTGTAAGCGCAGGGGAGACTCC
  TATGACCTCAAGGACTTTGTAAACCTGCACAGCTTCCAGGTTCGGGATGACTCTTCAGGA
  GACCGAGACAACAAGAAGTGGAGCCACATGTTCCTCCTGATCGAGGACCAAGGTGCCCAG
  GGCTATGAGCTGTTCTTCAAGACAAGAGAATTGAAGAAGAAGTGGATGGAGCAGTTTGAG
  ATGGCCATCTCCAACATCTATCCGGAGAATGCCACCGCCAACGGGCATGACTTCCAGATG
  TTCTCCTTTGAGGAGACCACATCCTGCAAGGCCTGTCAGATGCTGCTTAGAGGTACCTTC
  TATCAGGGCTACCGCTGCCATCGGTGCCGGGCATCTGCACACAAGGAGTGTCTGGGGAGG
  GTCCCTCCATGTGGCCGACATGGGCAAGATTTCCCAGGAACTATGAAGAAGGACAAACTA
  CATCGCAGGGCTCAGGACAAAAAGAGGAATGAGCTGGGTCTGCCCAAGATGGAGGTGTTT
  CAGGAATACTACGGGCTTCCTCCACCCCCTGGAGCCATTGGACCCTTTCTACGGCTCAAC
  CCTGGAGACATTGTGGAGCTCACGAAGGCTGAGGCTGAACAGAACTGGTGGGAGGGCAGA
  AATACATCTACTAATGAAATTGGCTGGTTTCCTTGTAACAGGGTGAAGCCCTATGTCCAT
  GGCCCTCCTCAGGACCTGTCTGTTCATCTCTGGTACGCAGGCCCCATGGAGCGGGCAGGG
  GCAGAGAGCATCCTGGCCAACCGCTCGGACGGGACTTTCTTGGTGCGGCAGAGGGTGAAG
  GATGCAGCAGAATTTGCCATCAGCATTAAATATAACGTCGAGGTCAAGCACATTAAAATC
  ATGACAGCAGAAGGACTGTACCGGATCACAGAGAAAAAGGCTTTCCGGGGGCTTACGGAG
  CTGGTGGAGTTTTACCAGCAGAACTCTCTAAAGGATTGCTTCAAGTCTCTGGACACCACC
  TTGCAGTTCCCCTTCAAGGAGCCTGAAAAGAGAACCATCAGCAGGCCAGCAGTGGGAAGC
  ACAAAGTATTTTGGCACAGCCAAAGCCCGCTATGACTTCTGCGCCCGAGACCGATCAGAG
  CTGTCGCTCAAGGAGGGTGACATCATCAAGATCCTTAACAAGAAGGGACAGCAAGGCTGG
  TGGCGAGGGGAGATCTATGGCCGGGTTGGCTGGTTCCCTGCCAACTACGTGGAGGAAGAT
  TATTCTGAATACTGCTGA
  
  Clone variation with respect to NM_005428.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005428 unedited
  CACGAGAATGGACTGTGGCGCCAATGCACCCACTGGCTCATCCAGTGCCGGGTGCTGCCG
  CCCAGCCACCGCGTGACCTGGGATGGGGCTCAGGTGTGTGAACTGGCCCAGGCCCTCCGG
  GATGGTGTCCTTCTGTGTCAGCTGCTTAACAACCTGCTACCCCATGCCATCAACCTGCGT
  GAGGTCAACCTGCGCCCCCAGATGTCCCAGTTCCTGTGCCTTAAGAACATTAGAACCTTC
  CTGTCCACCTGCTGTGAGAAGTTCGGCCTCAAGCGGAGCGAGCTCTTCGAAGCCTTTGAC
  CTCTTCGATGTGCAGGATTTTGGCAAGGTCATCTACACCCTGTCTGCTCTGTCCTGGACC
  CCGATCGCCCAGAACAGGGGGATCATGCCCTTCCCCACCGAGGAGGAGAGTGTAGGTGAT
  GAAGACATCTACAGTGGCCTGTCCGACCAGATCGACGACACGGTGGAGGAGGATGAGGAC
  CTGTATGACTGCGTGGAGAATGAGGAGGCGGAAGGCGACGAGATCTATGAGGACCTCATG
  CGCTCGGAGCCCGTGTCCATGCCGCCCAAGATGACAGAGTATGACAAGCGCTGCTGCTGC
  CTGCGGGAGATCCAGCAGACGGAGGAGAAGTACACTGACACGCTGGGCTCCATCCAGCAG
  CATTTCTTGAAGCCCCTGNCACGGTTCCTGAAACCTCAAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005428 unedited
  TTTTTGGGTTTGACTATGNNACNCGCGGCCGCATACTAGNGATCGATTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATATCCACCTTCATCCTTTTATTAA
  ATGGAACCCCCTTGAGCCCCGTTTGCTTGAGGGGACCCAAAAGAGAAATCGGGGTGTTAT
  AAATTAACCATGTCAGGGCACATCCCGGGAGCACCGCCAGCTGGACACTGGCCTCCTCCG
  TCCATCCCAAAATCTCCACCCGCCGGGAGCTGTCACAACCCCTGGGTCCGCTGCCTGCCC
  ACCCCGGGCTCAAAACCTGGAGTTTCTCGTCTCTTTGCCAAGGCCCCAGGGCTCAACAGT
  TTTCAAAATAATCTTCCTCCACGTAGTTGGCAGGGAACCAGCCAACCCGGCCATAGATTT
  CCCCTTGCCACCAACCTTGGTGTCCCTTTTTGTTAAGGATCTTGAAGAAGTCCCCCTCCT
  TGAGCCACAGCTCTGATACGGTCTCGGGCGCAAAAATATAGAGGGCTTTGGCTGTGCCAA
  AATACTTTGGGCTTCCCACTGGTGGCCTGGTGAAGGTTCTCTTTTCAGGCTCCTTGAAGG
  GGAACAGGAAGGTGGGTGTCCAAAAACTTGGAGCAACCCTTTAAAGAGTTCTGCCGGTAA
  AACACCACCAACTCCGTAAGCCCCCGGAAAACCTTTTTCTCTGTGATCCGGGACAATCCC
  TTTGGAGCCAGAATTTAAAGGGCCTGGCCCCGAACTTTTTTTTAATGGTGAAGGGAAAAT
  TTGTGGGTTCCTTAACCTTTTGGCGGACCAGAAAAACCCCGTTCTAAAGGGTGGGCCAGA
  AGCTTTTGGCCCTGGCCCCCCCCAGGGGGGCCGGGTACACAAAAATAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005428
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005428.2, NP_005419.2
• RefSeq Size: 2888 bp
• RefSeq ORF: 2538 bp
• Locus ID: 7409
• UniProt ID: P15498
• Cytogenetics: 19p13.3
• Domains: RhoGEF, SH2, SH3, CH, PH, DAG_PE-bind
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: B cell receptor signaling pathway, Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, T cell receptor signaling pathway
• Gene Summary: This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. The encoded protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. The encoded protein has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).