INSIG1 (NM_005542) Human Untagged Clone

CAT#: SC116677

INSIG1 (untagged)-Human insulin induced gene 1 (INSIG1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: INSIG1
• Synonyms: CL6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116677 sequence for NM_005542 edited (data generated by NextGen Sequencing)
  ATGCCCAGATTGCACGACCACTTCTGGAGCTGCTCCTGTGCGCACAGCGCGAGGCGCCGA
  GGCCCCCCGCGAGCCAGCGCCGCGGGGCTGGCGGCCAAGGTTGGGGAGATGATCAACGTT
  TCCGTGTCCGGGCCCTCCCTGCTGGCGGCCCACGGTGCCCCGGACGCTGACCCCGCGCCC
  AGGGGCCGCAGTGCTGCGATGAGCGGCCCCGAGCCCGGCAGCCCCTACCCCAACACCTGG
  CATCATCGCCTGTTGCAGAGGAGCCTCGTGCTCTTCTCGGTTGGGGTGGTCCTAGCCCTG
  GTGCTCAACCTGCTGCAGATCCAGAGGAATGTCACTCTCTTCCCCGAGGAGGTGATCGCC
  ACCATCTTTTCCTCCGCCTGGTGGGTCCCTCCCTGCTGCGGGACAGCAGCTGCTGTTGTT
  GGCCTACTGTACCCCTGTATCGACAGTCACCTCGGAGAACCCCACAAATTTAAGAGAGAA
  TGGGCCAGTGTCATGCGCTGCATAGCAGTTTTTGTTGGCATTAACCACGCCAGTGCTAAA
  TTGGATTTTGCCAATAATGTCCAGCTGTCCTTGACTTTAGCAGCCCTATCTTTGGGCCTT
  TGGTGGACATTTGATCGTTCCAGAAGTGGCCTTGGGCTGGGGATCACCATAGCTTTTCTA
  GCTACGCTGATCACGCAGTTTCTCGTGTATAATGGTGTCTATCAGTATACATCCCCAGAT
  TTCCTCTATATTCGTTCTTGGCTCCCTTGTATATTTTTCTCAGGAGGCGTCACGGTGGGG
  AACATAGGACGACAGTTAGCTATGGGTGTTCCTGAAAAGCCCCATAGTGATTGA
  
  Clone variation with respect to NM_005542.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005542 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGGCCGTGACTCCTCCTT
  TCCCCCGCCCCGCCTCCGTTCGGAGAGCCGGCGGGCGGGCGCCTCTCGGCCAGGAAGCGC
  CTCTTGGACGCGTGTGACCGATGCCCAGATTGCACGACCACTTCTGGAGCTGCTCCTGTG
  CGCACAGCGCGAGGCGCCGAGGCCCCCCGCGAGCCAGCGCCGCGGGGCTGGCGGCCAAGG
  TTGGGGAGATGATCAACGTTTCCGTGTCCGGGCCCTCCCTGCTGGCGGCCCACGGTGCCC
  CGGACGCTGACCCCGCGCCCAGGGGCCGCAGTGCTGCGATGAGCGGCCCCGAGCCCGGCA
  GCCCCTACCCCAACACCTGGCATCATCGCCTGTTGCAGAGGAGCCTCGTGCTCTTCTCGG
  TTGGGGTGGTCCTAGCCCTGGTGCTCAACCTGCTGCAGATCCAGAGGAATGTCACTCTCT
  TCCCCGAGGAGGTGATCGCCACCATCTTTTCCTCCGCCTGGTGGGTCCCTCCCTGCTGCG
  GGACAGCAGCTGCTGTTGTTGGCCTACTGTACCCCTGTATCGACAGTCACCTCGGGAGAC
  CCCACAAATTTAAGAGAGAATGGGCCAGTGTCATGCGCTGCATAGCAGTTTTTGGTGGCA
  TTAACCACGCCAGTGCTAANATGGATTTGCCAATATGTCCAGCTGTCCTTGACTTTAGCA
  GCCCTATCTTTGGGCCTTTGGTGGACATTTTGATCGTCCAGAAGTGGCCTTGGGGCTGGG
  ATCACCATAGCTTTTCTAGCTACGCTGATCACGCAGTTNCTCGGGTATAATGGGGTCTTC
  AGAATACATCCCCAAATTCCTCTATATTCCGTCTTGGCTTCCTTGGATATTTTCTCAGGA
  GGCGTCACGTGGGGAACCATAGACAACAGTACTTTGGTGGTTCTGAAAGCCCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005542 unedited
  CGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTTGAACCTTTAATAAAA
  GTAAAAAATGAATGCAAAAAGAACACAATGGTTGAAAACTTAGTATGAATGTGAACCTCA
  CTAGATGTTCAAATCTGGTAGAGTGCAAATTTTGTTCATACTATTTTACATTTTTACAAA
  CTCAAATCACTTTGGTTCATATATTTTCTATAAACTATTGGCAAAAAAATCCTCAAATTT
  ACATTCTTTTGGCTACATTATTTCTAACAGATATAGATTTACTTCCGGTTTCGGAGAGAA
  AGACTTATTGTGTGTGCGTGATCAAGTCTGTTTTAAAGATTCACTCGCTGCTTTCATCTA
  ATAACTTCTGGTTTTTCATAAAATGCTGACATCTTCATTGGAAATTTTTTTCATGTAACT
  GTTTTCATTTTCAGAAAATATATAAGGGGGTCATTCCAAAGTTCAGAATGATCCTATTTT
  TTTAAAAAACAAAATTCCTGTAAAACAAATTAACTCCAGGAACTTAAAATTTACTCCAAG
  ACATTTCCCTCAAAACAAAGCAAAAAACCCCAGCAAAGATCGTTACATCACAAAACCAAA
  CACAAAGACCAGCGCTCACAGGCAAGTTCCTCTAAGCTTCCATTCTGCTGACTGGTGGCT
  TCCATTTAAAAGGAGTCTTTTAATCAAGCCACTTTCACAGAATTTAAAACAAACCAAACA
  CATGTAAATTGCANAATACAAAAAGGTAAATTTATNAGTANAAATGACCCAAACCCACAA
  ACTGGAGTATTTCGAAGGTTGAGGGTTCAGTGGGAGGTTGTACACGAAAGGACTTTCACA
  CTGAAGAATCATGCCCGAGTTCCTCAGCAGACTGAATGAATGAGACCTTCTTCGACATTA
  CACGTAAAAATAATATTAGAATACAGCTANATGGNTTCTTTCCTGCACTGAGNTAGAACC
  AACTGCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005542
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005542.3, NP_005533.2
• RefSeq Size: 2978 bp
• RefSeq ORF: 834 bp
• Locus ID: 3638
• UniProt ID: O15503
• Cytogenetics: 7q36.3
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes an endoplasmic reticulum membrane protein that regulates cholesterol metabolism, lipogenesis, and glucose homeostasis. The encoded protein has six transmembrane helices which contain an effector protein binding site. It binds the sterol-sensing domains of sterol regulatory element-binding protein (SREBP) cleavage-activating protein (SCAP) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA reductase), and is essential for the sterol-mediated trafficking of these two proteins. It promotes the endoplasmic reticulum retention of SCAP and the ubiquitin-mediated degradation of HMG-CoA reductase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2016]
  Transcript Variant: This variant (1) encodes isoform 1. Variants 1 and 6 encode the same protein.