VAV3 (NM_006113) Human Untagged Clone

CAT#: SC116335

VAV3 (untagged)-Human vav 3 guanine nucleotide exchange factor (VAV3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAV3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006113 edited
  ATGGAGCCGTGGAAGCAGTGCGCGCAGTGGCTCATCCATTGCAAGGTGCTGCCCACCAAC
  CACCGGGTGACCTGGGACTCGGCTCAGGTGTTCGACCTTGCGCAGACCCTCCGCGATGGA
  GTCCTGCTCTGCCAGCTGCTTAACAACCTCCGGGCGCACTCCATCAACCTGAAGGAGATC
  AACCTGAGGCCGCAGATGTCCCAGTTTCTCTGTTTGAAGAACATAAGGACATTTCTCACG
  GCCTGTTGTGAGACGTTTGGAATGAGGAAAAGTGAACTTTTCGAGGCATTTGACTTGTTT
  GATGTTCGTGACTTTGGAAAGGTTATAGAAACATTATCACGACTTTCTCGAACACCTATA
  GCATTGGCCACAGGAATCAGGCCCTTCCCAACAGAAGAAAGCATTAATGATGAAGACATC
  TACAAAGGCCTTCCTGATTTAATAGATGAAACCCTTGTGGAAGATGAAGAAGATCTCTAT
  GACTGTGTTTATGGGGAAGATGAAGGTGGAGAAGTCTATGAGGACTTAATGAAGGCAGAG
  GAAGCACATCAGCCCAAATGTCCAGAAAATGATATACGAAGTTGTTGTCTAGCAGAAATT
  AAGCAGACAGAAGAAAAATATACAGAAACTTTGGAGTCAATAGAAAAGTATTTCATGGCA
  CCACTAAAAAGATTTCTGACAGCAGCAGAATTTGATTCAGTATTCATCAACATTCCTGAA
  CTTGTAAAACTTCATCGGAACCTAATGCAAGAGATTCATGATTCCATTGTAAATAAAAAT
  GACCAGAACTTGTACCAAGTTTTTATTAACTACAAGGAAAGATTGGTTATTTACGGGCAG
  TACTGCAGTGGAGTGGAGTCAGCCATCTCTAGTTTAGACTACATTTCTAAGACAAAAGAA
  GATGTCAAACTGAAATTAGAGGAATGTTCCAAAAGAGCAAATAATGGGAAATTTACTCTT
  CGAGACTTGCTTGTGGTTCCTATGCAACGTGTTTTAAAGTACCACCTTCTCCTCCAGGAA
  CTGGTCAAACATACCACTGATCCGACTGAGAAGGCAAATCTGAAACTGGCTCTTGATGCC
  ATGAAGGACTTGGCACAATATGTGAATGAAGTGAAAAGAGATAATGAGACCCTTCGTGAA
  ATTAAACAGTTTCAGCTATCTATAGAGAATTTGAACCAACCAGTTTTGCTTTTTGGACGA
  CCTCAGGGAGATGGTGAAATTCGAATAACCACTCTAGACAAGCATACCAAACAAGAAAGG
  CATATCTTCTTATTTGATTTGGCAGTGATCGTATGTAAGAGAAAAGGTGATAACTATGAA
  ATGAAGGAAATAATAGATCTTCAGCAGTACAAGATAGCCAATAATCCTACAACCGATAAA
  GAAAACAAAAAGTGGTCTTATGGCTTCTACCTCATCCATACCCAAGGACAAAATGGGTTA
  GAATTTTATTGCAAAACAAAAGATTTAAAGAAGAAATGGCTAGAACAGTTTGAAATGGCT
  TTGTCTAACATAAGACCAGACTATGCAGACTCCAATTTCCACGACTTCAAGATGCATACC
  TTCACTCGAGTCACATCCTGCAAAGTCTGCCAGATGCTCCTGAGGGGAACATTTTATCAA
  GGCTATTTATGTTTTAAGTGTGGAGCGAGAGCACACAAAGAATGTTTGGGAAGAGTAGAC
  AATTGTGGCAGAGTTAATTCTGGTGAACAAGGGACACTCAAACTACCAGAGAAACGGACC
  AATGGACTGCGAAGAACTCCTAAACAGGTGGATCCAGGTTTACCAAAGATGCAGGTCATT
  AGGAACTATTCTGGAACACCACCCCCAGCTCTGCATGAAGGACCCTCTTTACACCTCCAG
  GCCGGGGATACCGTTGAACTTCTGAAAGGAGATGCACACAGTCTGTTTTGGCAGGGCAGA
  AATTTAGCATCTGGAGAGGTTGGATTTTTTCCAAGTGATGCAGTCAAGCCTTGCCCATGT
  GTGCCCAAACCAGTAGATTATTCTTGCCAACCCTGGTATGCTGGAGCAATGGAAAGATTG
  CAAGCAGAGACCGAACTTATTAATAGGGTAAATAGTACTTACCTTGTGAGGCACAGGACC
  AAAGAGTCAGGAGAATATGCAATTAGCATTAAGTACAATAATGAAGCAAAGCACATCAAG
  ATTTTAACAAGAGATGGCTTTTTTCACATTGCAGAAAATAGAAAATTTAAAAGTTTAATG
  GAACTTGTGGAGTACTACAAGCATCATTCTCTCAAGGAAGGGTTCAGAACCTTAGATACA
  ACTCTGCAGTTTCCATACAAGGAGCCAGAACATTCAGCTGGACAGAGGGGTAATAGAGCA
  GGCAACAGCTTGTTAAGTCCAAAAGTGCTGGGCATTGCCATCGCTCGGTATGACTTCTGT
  GCAAGAGATATGAGAGAGTTGTCCTTGTTGAAAGGAGATGTGGTGAAGATTTACACAAAG
  ATGAGTGCAAATGGCTGGTGGAGAGGAGAAGTAAATGGCAGGGTGGGCTGGTTTCCATCC
  ACATATGTGGAAGAGGATGAATAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006113 unedited
  CACCCATTCCGCACGAGGGCGGCAGCCGCCGCCGAGCCGGCCGGGGCGCACGGAGAGCGC
  GCGGGACTCGCTGCAGCGGCGGCCGGGTCGCGGCGCACCCGGGCCGGGACCGGAGCCGAG
  CCTAGCGCGGCGCCCGCGACCCGTCAGCCGCGGCTCCTGCTCCCTCGATCCCGCGCTGTT
  ATGGGCCGGCGGCTGTTGGCGTCGGCGGGGCGCGGAGGAACCGCGGCGGCGGCGGCGGCG
  GCCGCATCCTTGCCGCCCGCCCCGGCCCAGCCGCGTCCCGGAGCCGTCGGGCATGGAGCC
  GTGGAAGCAGTGCGCGCAGTGGCTCATCCATTGCAAGGTGCTGCCCACCAACCACCGGGT
  GACCTGGGACTCGGCTCAGGTGTTCGACCTTGCGCAGACCCTCCGCGATGGAGTCCTGCT
  CTGCCAGCTGCTTAACAACCTCCGGGCGCACTCCATCAACCTGAAGGAGATCAACCTGAG
  GCCGCAGATGTCCCAGTTTCTCTGTTTGAAGAACATAAGGACATTTCTCACGGCCTGTTG
  TGAGACGTTTGGAATGAGGAAAAGTGAACTTTTCGAGGCATTTGACTTGTTTGATGTTCG
  TGACTTTGGAAAGGTTATAGAAACATTATCACGACTTTCTCGAACACCTATAGCATTGGC
  CACAGGAATCAGGCCCTTCCCAACAGAAGAAAGCATTAATGATGAAGACATCTACAAAGG
  CCTTCCTGATTTAATAGATGAAACCCTTGTGGAAGATGAAGAAGATCTCTATGACTGTGT
  TTATGGGGAAAGATGAAGTGGAGGAGTCTATGAGGACTTNNATGAGGCAGAGGAAGCACA
  TCAGCCCANATGTNCCAGAAATGATATACGAAGTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006113 unedited
  CCGCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTCAATTTAGTCACT
  ATTTATTATATTGACATATTTACAAAATAATACAAAGTGAAATACCACTCTAATTCACCG
  TATTACACGAGGGCTGCATACAGGCAAGACAAAGTATATGGAAAACATTTACTTCTGTCT
  TTGGTATTAGAACTCTACACAAATCTGCAGCATTTAAATTTTCCAAAACAAAGTATTAAA
  CGTGGACAAAGATGTAATTGGTAATGTCACAAAAAGGGGCTCCAATATCCTCTGCTAGGA
  AACCCCCAGGCCCATGAAATGCAACAGGAAGACTAAACACCATTTATAAGGAGAGGGTCT
  ATTGACTAAAATAAACAATACATGCTACAATACCATCCACAGGAGTGTTTCTGCTTGTGT
  GAGGCTGCTCCCTCCATAACAAAGTTCGGCTGACGGCAACAGACAAAACATTCCCGAGAC
  AGAGTCGCAGATAAGACTTTACAGGAAAGAATCCATTCCAAGAGTACACTTTGAGGTGTA
  ACTTTATTGTACAGAACATTTTATAAAATACATTNTTGTGATCATTTACACATATACAAA
  GACTTAAATGGTTTTTAGCAAATATTATTTNTTCTAAACACAATCACAGTTTACATAATT
  CTGAGGTAAAGGTCTTGATTCTATCCTAGATGAAAAGTCCTACCCCATGAGGGTCTCTAC
  AGGGGGATGTGTCCACTGACCCTGAGTTCTGCCCCCANACCGATGATGACAGGGCAGCTA
  CACTGCTCATTTCAGGATGATCAAATTTCAAACTAAAGCTCTGTTCAGTCTACAAGTAAG
  CTTCTTTTTTCCAACAGAACTTCTAATCTGCAGACAAACTTTGTTTGGAGGAAATTCCAT
  CAGAAGCCCANTAGGGTTACTGACTGCTGATGGGCTCTGACCCAGAGATGTTTAGCTCTC
  AAAAAAAGAGGCTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006113
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: no
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006113.3, NP_006104.3
• RefSeq Size: 4768 bp
• RefSeq ORF: 2544 bp
• Locus ID: 10451
• UniProt ID: Q9UKW4
• Cytogenetics: 1p13.3
• Domains: RhoGEF, SH2, SH3, CH, PH, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, Chemokine signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, T cell receptor signaling pathway
• Gene Summary: This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1, also known as VAV-3alpha) encodes the longer isoform (1).